Gordonia phage Luker
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A514A4W6|A0A514A4W6_9CAUD Uncharacterized protein OS=Gordonia phage Luker OX=2591188 GN=59 PE=4 SV=1
MM1 pKa = 7.33 QGPPYY6 pKa = 10.04 YY7 pKa = 10.55 AGPEE11 pKa = 3.77 EE12 pKa = 4.42 LGYY15 pKa = 8.86 YY16 pKa = 8.06 TYY18 pKa = 10.35 PDD20 pKa = 3.34 SDD22 pKa = 3.95 MDD24 pKa = 3.71 EE25 pKa = 3.78 LAYY28 pKa = 10.42 RR29 pKa = 11.84 IRR31 pKa = 11.84 SRR33 pKa = 11.84 LIPIWDD39 pKa = 3.8 EE40 pKa = 4.39 LEE42 pKa = 4.32 VPLLPEE48 pKa = 3.8 EE49 pKa = 4.3 EE50 pKa = 4.73 EE51 pKa = 3.94 IVEE54 pKa = 4.09 ATEE57 pKa = 4.01 FF58 pKa = 3.55
Molecular weight: 6.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.865
IPC2_protein 3.808
IPC_protein 3.656
Toseland 3.503
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.516
Rodwell 3.503
Grimsley 3.427
Solomon 3.579
Lehninger 3.528
Nozaki 3.757
DTASelect 3.821
Thurlkill 3.554
EMBOSS 3.528
Sillero 3.77
Patrickios 0.299
IPC_peptide 3.592
IPC2_peptide 3.745
IPC2.peptide.svr19 3.703
Protein with the highest isoelectric point:
>tr|A0A514A4Y8|A0A514A4Y8_9CAUD Uncharacterized protein OS=Gordonia phage Luker OX=2591188 GN=79 PE=4 SV=1
MM1 pKa = 7.2 KK2 pKa = 10.46 HH3 pKa = 6.32 NINGHH8 pKa = 5.38 GYY10 pKa = 10.29 GVLRR14 pKa = 11.84 DD15 pKa = 3.8 VKK17 pKa = 8.93 VTARR21 pKa = 11.84 QSVTATRR28 pKa = 11.84 PTPLSANLGNTGAEE42 pKa = 4.15 LNQIDD47 pKa = 4.63 ANNDD51 pKa = 2.93 EE52 pKa = 5.76 AINKK56 pKa = 8.28 NNWHH60 pKa = 6.8 LGGDD64 pKa = 4.27 PIVSNDD70 pKa = 3.38 PDD72 pKa = 3.28 EE73 pKa = 4.5 LARR76 pKa = 11.84 AFYY79 pKa = 10.68 AKK81 pKa = 10.18 RR82 pKa = 11.84 EE83 pKa = 4.02 RR84 pKa = 11.84 EE85 pKa = 3.89 RR86 pKa = 11.84 MIEE89 pKa = 4.51 DD90 pKa = 3.3 IQDD93 pKa = 3.37 APVIRR98 pKa = 11.84 TRR100 pKa = 11.84 PVRR103 pKa = 11.84 PAPKK107 pKa = 10.16 KK108 pKa = 8.89 SDD110 pKa = 3.12 SRR112 pKa = 11.84 IARR115 pKa = 11.84 EE116 pKa = 3.52 RR117 pKa = 11.84 ADD119 pKa = 3.68 RR120 pKa = 11.84 RR121 pKa = 11.84 RR122 pKa = 11.84 ARR124 pKa = 11.84 NKK126 pKa = 9.79 ARR128 pKa = 11.84 LARR131 pKa = 11.84 RR132 pKa = 11.84 RR133 pKa = 11.84 AAA135 pKa = 3.82
Molecular weight: 15.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.29
IPC2_protein 9.692
IPC_protein 10.877
Toseland 11.096
ProMoST 11.316
Dawson 11.125
Bjellqvist 10.979
Wikipedia 11.477
Rodwell 11.111
Grimsley 11.169
Solomon 11.462
Lehninger 11.389
Nozaki 11.082
DTASelect 10.979
Thurlkill 11.096
EMBOSS 11.55
Sillero 11.096
Patrickios 10.847
IPC_peptide 11.477
IPC2_peptide 10.277
IPC2.peptide.svr19 9.094
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
93
0
93
22763
33
3323
244.8
26.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.823 ± 0.961
0.931 ± 0.14
6.062 ± 0.309
6.888 ± 0.344
3.523 ± 0.13
7.952 ± 0.295
1.634 ± 0.171
5.285 ± 0.213
5.078 ± 0.253
7.35 ± 0.232
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.5 ± 0.146
4.059 ± 0.141
5.228 ± 0.335
3.703 ± 0.221
5.733 ± 0.353
6.229 ± 0.193
6.142 ± 0.302
7.148 ± 0.216
1.713 ± 0.124
3.018 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here