Amino acid dipepetide frequency for Bacillus sp. P16(2019)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.084AlaAla: 6.084 ± 0.094	0.643AlaCys: 0.643 ± 0.023	3.739AlaAsp: 3.739 ± 0.063	5.198AlaGlu: 5.198 ± 0.068	3.406AlaPhe: 3.406 ± 0.061	5.573AlaGly: 5.573 ± 0.088	1.445AlaHis: 1.445 ± 0.035	5.822AlaIle: 5.822 ± 0.083	3.969AlaLys: 3.969 ± 0.063	7.636AlaLeu: 7.636 ± 0.093	2.031AlaMet: 2.031 ± 0.045	2.787AlaAsn: 2.787 ± 0.058	2.167AlaPro: 2.167 ± 0.052	2.492AlaGln: 2.492 ± 0.045	2.753AlaArg: 2.753 ± 0.048	4.63AlaSer: 4.63 ± 0.072	3.944AlaThr: 3.944 ± 0.137	5.509AlaVal: 5.509 ± 0.08	0.661AlaTrp: 0.661 ± 0.024	2.397AlaTyr: 2.397 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.451CysAla: 0.451 ± 0.021	0.077CysCys: 0.077 ± 0.008	0.337CysAsp: 0.337 ± 0.017	0.424CysGlu: 0.424 ± 0.02	0.301CysPhe: 0.301 ± 0.016	0.632CysGly: 0.632 ± 0.027	0.188CysHis: 0.188 ± 0.012	0.467CysIle: 0.467 ± 0.023	0.31CysLys: 0.31 ± 0.016	0.684CysLeu: 0.684 ± 0.024	0.181CysMet: 0.181 ± 0.013	0.241CysAsn: 0.241 ± 0.014	0.309CysPro: 0.309 ± 0.017	0.25CysGln: 0.25 ± 0.014	0.279CysArg: 0.279 ± 0.016	0.485CysSer: 0.485 ± 0.02	0.372CysThr: 0.372 ± 0.019	0.44CysVal: 0.44 ± 0.017	0.067CysTrp: 0.067 ± 0.007	0.234CysTyr: 0.234 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.477AspAla: 3.477 ± 0.051	0.314AspCys: 0.314 ± 0.017	2.506AspAsp: 2.506 ± 0.06	4.655AspGlu: 4.655 ± 0.083	2.309AspPhe: 2.309 ± 0.048	3.521AspGly: 3.521 ± 0.065	1.344AspHis: 1.344 ± 0.039	4.006AspIle: 4.006 ± 0.062	2.453AspLys: 2.453 ± 0.038	5.242AspLeu: 5.242 ± 0.067	1.387AspMet: 1.387 ± 0.032	1.67AspAsn: 1.67 ± 0.042	2.043AspPro: 2.043 ± 0.041	2.586AspGln: 2.586 ± 0.05	2.268AspArg: 2.268 ± 0.049	2.879AspSer: 2.879 ± 0.053	2.61AspThr: 2.61 ± 0.048	3.961AspVal: 3.961 ± 0.061	0.7AspTrp: 0.7 ± 0.021	2.084AspTyr: 2.084 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	6.059GluAla: 6.059 ± 0.082	0.363GluCys: 0.363 ± 0.019	4.201GluAsp: 4.201 ± 0.069	7.523GluGlu: 7.523 ± 0.105	2.447GluPhe: 2.447 ± 0.05	4.665GluGly: 4.665 ± 0.072	1.725GluHis: 1.725 ± 0.037	4.87GluIle: 4.87 ± 0.062	4.912GluLys: 4.912 ± 0.08	7.895GluLeu: 7.895 ± 0.105	2.243GluMet: 2.243 ± 0.048	3.139GluAsn: 3.139 ± 0.053	2.317GluPro: 2.317 ± 0.049	4.186GluGln: 4.186 ± 0.072	3.76GluArg: 3.76 ± 0.069	3.964GluSer: 3.964 ± 0.058	3.966GluThr: 3.966 ± 0.063	5.118GluVal: 5.118 ± 0.079	0.967GluTrp: 0.967 ± 0.031	2.266GluTyr: 2.266 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	3.007PheAla: 3.007 ± 0.06	0.321PheCys: 0.321 ± 0.017	2.334PheAsp: 2.334 ± 0.043	2.865PheGlu: 2.865 ± 0.053	2.237PhePhe: 2.237 ± 0.055	3.095PheGly: 3.095 ± 0.058	0.936PheHis: 0.936 ± 0.028	3.798PheIle: 3.798 ± 0.076	2.162PheLys: 2.162 ± 0.043	4.487PheLeu: 4.487 ± 0.077	1.131PheMet: 1.131 ± 0.033	1.744PheAsn: 1.744 ± 0.044	1.542PhePro: 1.542 ± 0.036	1.567PheGln: 1.567 ± 0.04	1.535PheArg: 1.535 ± 0.039	3.323PheSer: 3.323 ± 0.05	2.699PheThr: 2.699 ± 0.044	3.112PheVal: 3.112 ± 0.053	0.494PheTrp: 0.494 ± 0.019	1.705PheTyr: 1.705 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	5.31GlyAla: 5.31 ± 0.138	0.61GlyCys: 0.61 ± 0.024	3.218GlyAsp: 3.218 ± 0.053	4.655GlyGlu: 4.655 ± 0.073	3.412GlyPhe: 3.412 ± 0.061	4.84GlyGly: 4.84 ± 0.087	1.456GlyHis: 1.456 ± 0.04	5.465GlyIle: 5.465 ± 0.084	3.931GlyLys: 3.931 ± 0.074	7.027GlyLeu: 7.027 ± 0.09	2.173GlyMet: 2.173 ± 0.044	2.364GlyAsn: 2.364 ± 0.046	1.868GlyPro: 1.868 ± 0.058	2.478GlyGln: 2.478 ± 0.053	2.752GlyArg: 2.752 ± 0.049	4.146GlySer: 4.146 ± 0.066	4.09GlyThr: 4.09 ± 0.061	5.15GlyVal: 5.15 ± 0.067	0.842GlyTrp: 0.842 ± 0.031	2.744GlyTyr: 2.744 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	1.536HisAla: 1.536 ± 0.035	0.191HisCys: 0.191 ± 0.012	1.112HisAsp: 1.112 ± 0.03	1.553HisGlu: 1.553 ± 0.038	1.079HisPhe: 1.079 ± 0.032	1.451HisGly: 1.451 ± 0.033	0.767HisHis: 0.767 ± 0.024	1.586HisIle: 1.586 ± 0.037	1.036HisLys: 1.036 ± 0.031	2.341HisLeu: 2.341 ± 0.04	0.509HisMet: 0.509 ± 0.02	0.795HisAsn: 0.795 ± 0.028	1.134HisPro: 1.134 ± 0.032	1.1HisGln: 1.1 ± 0.03	0.937HisArg: 0.937 ± 0.028	1.574HisSer: 1.574 ± 0.038	1.326HisThr: 1.326 ± 0.031	1.653HisVal: 1.653 ± 0.035	0.253HisTrp: 0.253 ± 0.013	0.908HisTyr: 0.908 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.639IleAla: 5.639 ± 0.078	0.605IleCys: 0.605 ± 0.026	4.085IleAsp: 4.085 ± 0.063	5.475IleGlu: 5.475 ± 0.077	2.965IlePhe: 2.965 ± 0.066	5.762IleGly: 5.762 ± 0.076	1.785IleHis: 1.785 ± 0.038	5.416IleIle: 5.416 ± 0.089	3.815IleLys: 3.815 ± 0.059	6.955IleLeu: 6.955 ± 0.093	1.764IleMet: 1.764 ± 0.037	3.003IleAsn: 3.003 ± 0.054	3.243IlePro: 3.243 ± 0.053	3.088IleGln: 3.088 ± 0.052	3.174IleArg: 3.174 ± 0.05	4.991IleSer: 4.991 ± 0.067	4.137IleThr: 4.137 ± 0.06	5.108IleVal: 5.108 ± 0.069	0.726IleTrp: 0.726 ± 0.023	2.368IleTyr: 2.368 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	4.156LysAla: 4.156 ± 0.071	0.222LysCys: 0.222 ± 0.013	3.191LysAsp: 3.191 ± 0.062	5.723LysGlu: 5.723 ± 0.086	1.27LysPhe: 1.27 ± 0.035	3.689LysGly: 3.689 ± 0.056	1.324LysHis: 1.324 ± 0.037	3.077LysIle: 3.077 ± 0.061	4.542LysLys: 4.542 ± 0.076	5.014LysLeu: 5.014 ± 0.073	1.607LysMet: 1.607 ± 0.037	2.279LysAsn: 2.279 ± 0.05	2.127LysPro: 2.127 ± 0.041	3.471LysGln: 3.471 ± 0.062	3.219LysArg: 3.219 ± 0.055	3.065LysSer: 3.065 ± 0.055	2.845LysThr: 2.845 ± 0.053	3.823LysVal: 3.823 ± 0.054	0.665LysTrp: 0.665 ± 0.023	1.465LysTyr: 1.465 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	7.847LeuAla: 7.847 ± 0.088	0.695LeuCys: 0.695 ± 0.028	5.279LeuAsp: 5.279 ± 0.074	6.923LeuGlu: 6.923 ± 0.086	4.97LeuPhe: 4.97 ± 0.083	6.415LeuGly: 6.415 ± 0.08	2.237LeuHis: 2.237 ± 0.046	7.668LeuIle: 7.668 ± 0.104	5.643LeuLys: 5.643 ± 0.068	10.86LeuLeu: 10.86 ± 0.129	2.591LeuMet: 2.591 ± 0.046	4.11LeuAsn: 4.11 ± 0.057	4.076LeuPro: 4.076 ± 0.065	3.829LeuGln: 3.829 ± 0.06	3.953LeuArg: 3.953 ± 0.059	7.304LeuSer: 7.304 ± 0.096	6.197LeuThr: 6.197 ± 0.075	6.645LeuVal: 6.645 ± 0.078	0.867LeuTrp: 0.867 ± 0.027	3.286LeuTyr: 3.286 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.057MetAla: 2.057 ± 0.041	0.132MetCys: 0.132 ± 0.01	1.454MetAsp: 1.454 ± 0.034	1.815MetGlu: 1.815 ± 0.042	1.058MetPhe: 1.058 ± 0.034	1.696MetGly: 1.696 ± 0.038	0.48MetHis: 0.48 ± 0.02	2.131MetIle: 2.131 ± 0.044	2.058MetLys: 2.058 ± 0.044	2.618MetLeu: 2.618 ± 0.048	0.889MetMet: 0.889 ± 0.03	1.629MetAsn: 1.629 ± 0.035	0.991MetPro: 0.991 ± 0.032	0.987MetGln: 0.987 ± 0.027	1.15MetArg: 1.15 ± 0.029	1.852MetSer: 1.852 ± 0.037	1.744MetThr: 1.744 ± 0.036	1.781MetVal: 1.781 ± 0.043	0.201MetTrp: 0.201 ± 0.013	0.82MetTyr: 0.82 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.505AsnAla: 2.505 ± 0.041	0.216AsnCys: 0.216 ± 0.014	2.036AsnAsp: 2.036 ± 0.047	3.447AsnGlu: 3.447 ± 0.057	1.304AsnPhe: 1.304 ± 0.031	2.948AsnGly: 2.948 ± 0.056	1.082AsnHis: 1.082 ± 0.026	2.757AsnIle: 2.757 ± 0.05	2.206AsnLys: 2.206 ± 0.045	3.501AsnLeu: 3.501 ± 0.056	1.106AsnMet: 1.106 ± 0.031	1.662AsnAsn: 1.662 ± 0.048	1.889AsnPro: 1.889 ± 0.037	2.437AsnGln: 2.437 ± 0.048	1.981AsnArg: 1.981 ± 0.039	2.08AsnSer: 2.08 ± 0.041	2.035AsnThr: 2.035 ± 0.044	2.747AsnVal: 2.747 ± 0.05	0.546AsnTrp: 0.546 ± 0.022	1.355AsnTyr: 1.355 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	2.458ProAla: 2.458 ± 0.045	0.196ProCys: 0.196 ± 0.013	2.117ProAsp: 2.117 ± 0.047	3.168ProGlu: 3.168 ± 0.051	1.985ProPhe: 1.985 ± 0.043	2.398ProGly: 2.398 ± 0.047	0.864ProHis: 0.864 ± 0.025	2.844ProIle: 2.844 ± 0.043	1.784ProLys: 1.784 ± 0.041	3.708ProLeu: 3.708 ± 0.055	0.853ProMet: 0.853 ± 0.025	1.547ProAsn: 1.547 ± 0.031	1.096ProPro: 1.096 ± 0.035	1.12ProGln: 1.12 ± 0.03	1.169ProArg: 1.169 ± 0.029	2.404ProSer: 2.404 ± 0.039	2.162ProThr: 2.162 ± 0.058	2.766ProVal: 2.766 ± 0.049	0.406ProTrp: 0.406 ± 0.018	1.376ProTyr: 1.376 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.39GlnAla: 3.39 ± 0.056	0.21GlnCys: 0.21 ± 0.012	1.91GlnAsp: 1.91 ± 0.035	3.324GlnGlu: 3.324 ± 0.06	1.715GlnPhe: 1.715 ± 0.037	2.377GlnGly: 2.377 ± 0.043	0.957GlnHis: 0.957 ± 0.028	2.825GlnIle: 2.825 ± 0.041	2.586GlnLys: 2.586 ± 0.047	4.79GlnLeu: 4.79 ± 0.075	1.207GlnMet: 1.207 ± 0.032	1.523GlnAsn: 1.523 ± 0.037	1.467GlnPro: 1.467 ± 0.036	2.117GlnGln: 2.117 ± 0.048	1.842GlnArg: 1.842 ± 0.047	2.774GlnSer: 2.774 ± 0.05	2.603GlnThr: 2.603 ± 0.053	2.752GlnVal: 2.752 ± 0.048	0.491GlnTrp: 0.491 ± 0.023	1.33GlnTyr: 1.33 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.734ArgAla: 2.734 ± 0.05	0.253ArgCys: 0.253 ± 0.013	2.167ArgAsp: 2.167 ± 0.043	3.23ArgGlu: 3.23 ± 0.055	1.968ArgPhe: 1.968 ± 0.04	2.47ArgGly: 2.47 ± 0.044	0.876ArgHis: 0.876 ± 0.03	3.101ArgIle: 3.101 ± 0.054	2.657ArgLys: 2.657 ± 0.052	4.458ArgLeu: 4.458 ± 0.068	1.363ArgMet: 1.363 ± 0.034	1.753ArgAsn: 1.753 ± 0.037	1.401ArgPro: 1.401 ± 0.038	1.632ArgGln: 1.632 ± 0.041	2.015ArgArg: 2.015 ± 0.046	2.585ArgSer: 2.585 ± 0.052	2.319ArgThr: 2.319 ± 0.045	3.011ArgVal: 3.011 ± 0.051	0.478ArgTrp: 0.478 ± 0.021	1.605ArgTyr: 1.605 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.944SerAla: 3.944 ± 0.066	0.407SerCys: 0.407 ± 0.019	3.088SerAsp: 3.088 ± 0.05	4.41SerGlu: 4.41 ± 0.066	3.504SerPhe: 3.504 ± 0.055	4.686SerGly: 4.686 ± 0.068	1.396SerHis: 1.396 ± 0.033	5.189SerIle: 5.189 ± 0.066	3.563SerLys: 3.563 ± 0.066	6.601SerLeu: 6.601 ± 0.098	1.901SerMet: 1.901 ± 0.038	2.525SerAsn: 2.525 ± 0.048	2.199SerPro: 2.199 ± 0.043	2.21SerGln: 2.21 ± 0.043	2.502SerArg: 2.502 ± 0.049	4.377SerSer: 4.377 ± 0.066	3.597SerThr: 3.597 ± 0.062	4.446SerVal: 4.446 ± 0.065	0.751SerTrp: 0.751 ± 0.026	2.436SerTyr: 2.436 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	4.177ThrAla: 4.177 ± 0.06	0.371ThrCys: 0.371 ± 0.021	2.968ThrAsp: 2.968 ± 0.049	3.898ThrGlu: 3.898 ± 0.067	2.792ThrPhe: 2.792 ± 0.048	4.437ThrGly: 4.437 ± 0.198	1.321ThrHis: 1.321 ± 0.036	4.597ThrIle: 4.597 ± 0.064	2.966ThrLys: 2.966 ± 0.055	5.814ThrLeu: 5.814 ± 0.076	1.391ThrMet: 1.391 ± 0.034	2.377ThrAsn: 2.377 ± 0.045	2.278ThrPro: 2.278 ± 0.047	1.795ThrGln: 1.795 ± 0.038	2.038ThrArg: 2.038 ± 0.042	3.612ThrSer: 3.612 ± 0.056	3.111ThrThr: 3.111 ± 0.056	4.055ThrVal: 4.055 ± 0.065	0.589ThrTrp: 0.589 ± 0.023	2.062ThrTyr: 2.062 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	5.221ValAla: 5.221 ± 0.083	0.562ValCys: 0.562 ± 0.023	3.84ValAsp: 3.84 ± 0.063	5.099ValGlu: 5.099 ± 0.074	3.114ValPhe: 3.114 ± 0.055	4.774ValGly: 4.774 ± 0.063	1.532ValHis: 1.532 ± 0.035	5.36ValIle: 5.36 ± 0.071	3.762ValLys: 3.762 ± 0.064	6.927ValLeu: 6.927 ± 0.084	1.995ValMet: 1.995 ± 0.036	2.837ValAsn: 2.837 ± 0.055	2.646ValPro: 2.646 ± 0.044	2.567ValGln: 2.567 ± 0.05	2.722ValArg: 2.722 ± 0.059	4.765ValSer: 4.765 ± 0.069	4.418ValThr: 4.418 ± 0.068	4.946ValVal: 4.946 ± 0.075	0.711ValTrp: 0.711 ± 0.026	2.302ValTyr: 2.302 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.638TrpAla: 0.638 ± 0.026	0.068TrpCys: 0.068 ± 0.007	0.523TrpAsp: 0.523 ± 0.023	0.682TrpGlu: 0.682 ± 0.024	0.552TrpPhe: 0.552 ± 0.022	0.693TrpGly: 0.693 ± 0.026	0.252TrpHis: 0.252 ± 0.015	0.837TrpIle: 0.837 ± 0.024	0.649TrpLys: 0.649 ± 0.023	1.291TrpLeu: 1.291 ± 0.038	0.384TrpMet: 0.384 ± 0.017	0.537TrpAsn: 0.537 ± 0.019	0.316TrpPro: 0.316 ± 0.017	0.467TrpGln: 0.467 ± 0.021	0.491TrpArg: 0.491 ± 0.022	0.721TrpSer: 0.721 ± 0.028	0.625TrpThr: 0.625 ± 0.022	0.735TrpVal: 0.735 ± 0.025	0.153TrpTrp: 0.153 ± 0.013	0.354TrpTyr: 0.354 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.193TyrAla: 2.193 ± 0.039	0.272TyrCys: 0.272 ± 0.015	1.907TyrAsp: 1.907 ± 0.04	2.519TyrGlu: 2.519 ± 0.045	1.702TyrPhe: 1.702 ± 0.041	2.413TyrGly: 2.413 ± 0.042	0.876TyrHis: 0.876 ± 0.026	2.29TyrIle: 2.29 ± 0.042	1.772TyrLys: 1.772 ± 0.04	3.547TyrLeu: 3.547 ± 0.053	0.837TyrMet: 0.837 ± 0.025	1.319TyrAsn: 1.319 ± 0.036	1.313TyrPro: 1.313 ± 0.036	1.81TyrGln: 1.81 ± 0.044	1.533TyrArg: 1.533 ± 0.039	2.203TyrSer: 2.203 ± 0.044	1.848TyrThr: 1.848 ± 0.039	2.341TyrVal: 2.341 ± 0.042	0.4TyrTrp: 0.4 ± 0.019	1.428TyrTyr: 1.428 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4393 proteins (1227302 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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