Mycoplasma penetrans (strain HF-2)
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1028 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8EUZ1|Q8EUZ1_MYCPE Transcriptional regulator DeoR family protein OS=Mycoplasma penetrans (strain HF-2) OX=272633 GN=MYPE7770 PE=4 SV=1
MM1 pKa = 7.46 VEE3 pKa = 4.43 PNEE6 pKa = 3.88 FDD8 pKa = 3.46 QFEE11 pKa = 4.21 FDD13 pKa = 4.24 QSYY16 pKa = 10.51 DD17 pKa = 3.32 QNLFYY22 pKa = 10.32 PQEE25 pKa = 4.26 YY26 pKa = 9.86 VDD28 pKa = 3.81 TVQYY32 pKa = 8.05 YY33 pKa = 9.0 QPIEE37 pKa = 4.59 SIHH40 pKa = 6.24 DD41 pKa = 3.56 QQFEE45 pKa = 4.32 QPVEE49 pKa = 4.07 YY50 pKa = 11.03 VQDD53 pKa = 3.63 QQFEE57 pKa = 4.45 QPVEE61 pKa = 4.07 YY62 pKa = 11.04 VQDD65 pKa = 3.52 QQFQQPVEE73 pKa = 4.11 YY74 pKa = 10.74 VQTQFDD80 pKa = 3.78 QNIQQTPQNEE90 pKa = 3.83 NAEE93 pKa = 4.49 YY94 pKa = 10.18 IDD96 pKa = 3.6 TSSWTEE102 pKa = 3.4 QDD104 pKa = 3.75 FINYY108 pKa = 7.38 YY109 pKa = 10.4 AQVDD113 pKa = 4.42 FYY115 pKa = 11.72 NKK117 pKa = 9.49 YY118 pKa = 9.66 PNAFVYY124 pKa = 9.77 QGKK127 pKa = 9.89 IYY129 pKa = 10.89 NSDD132 pKa = 3.34 LDD134 pKa = 3.53 QYY136 pKa = 10.61 VPSKK140 pKa = 10.78 IGDD143 pKa = 3.86 LPNEE147 pKa = 4.23 PNLQIVKK154 pKa = 10.38 APEE157 pKa = 3.84 FEE159 pKa = 4.21 EE160 pKa = 5.27 KK161 pKa = 10.39 SIKK164 pKa = 10.75 RR165 pKa = 11.84 NDD167 pKa = 3.4 FLKK170 pKa = 11.24 NNN172 pKa = 3.9
Molecular weight: 20.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.852
IPC2_protein 3.757
IPC_protein 3.719
Toseland 3.528
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.554
Grimsley 3.439
Solomon 3.694
Lehninger 3.643
Nozaki 3.821
DTASelect 3.999
Thurlkill 3.567
EMBOSS 3.63
Sillero 3.834
Patrickios 0.159
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|Q8EUE0|Q8EUE0_MYCPE Aminopeptidase OS=Mycoplasma penetrans (strain HF-2) OX=272633 GN=MYPE9890 PE=3 SV=1
MM1 pKa = 7.44 ARR3 pKa = 11.84 ILGVDD8 pKa = 3.26 IPNNKK13 pKa = 9.09 RR14 pKa = 11.84 VVISLTYY21 pKa = 9.96 IFGIGKK27 pKa = 9.3 SRR29 pKa = 11.84 SQEE32 pKa = 3.73 ILSKK36 pKa = 11.22 AKK38 pKa = 9.61 IDD40 pKa = 3.76 EE41 pKa = 4.28 NKK43 pKa = 10.01 KK44 pKa = 9.98 VSALSEE50 pKa = 4.31 EE51 pKa = 4.22 EE52 pKa = 3.85 LATIRR57 pKa = 11.84 KK58 pKa = 8.47 IASEE62 pKa = 4.06 YY63 pKa = 10.55 VIEE66 pKa = 4.98 GDD68 pKa = 3.92 LRR70 pKa = 11.84 RR71 pKa = 11.84 EE72 pKa = 3.5 VAMNIKK78 pKa = 10.13 RR79 pKa = 11.84 LMEE82 pKa = 4.52 IGSYY86 pKa = 9.99 RR87 pKa = 11.84 GIRR90 pKa = 11.84 HH91 pKa = 5.92 RR92 pKa = 11.84 RR93 pKa = 11.84 NLPVRR98 pKa = 11.84 GQRR101 pKa = 11.84 TKK103 pKa = 11.11 CNARR107 pKa = 11.84 TRR109 pKa = 11.84 KK110 pKa = 9.89 GPRR113 pKa = 11.84 KK114 pKa = 7.53 TVANKK119 pKa = 10.33 KK120 pKa = 9.81 IEE122 pKa = 4.29 TKK124 pKa = 10.69
Molecular weight: 14.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.765
IPC_protein 10.482
Toseland 10.965
ProMoST 10.672
Dawson 11.023
Bjellqvist 10.716
Wikipedia 11.228
Rodwell 11.33
Grimsley 11.052
Solomon 11.169
Lehninger 11.14
Nozaki 10.935
DTASelect 10.716
Thurlkill 10.95
EMBOSS 11.374
Sillero 10.965
Patrickios 11.052
IPC_peptide 11.184
IPC2_peptide 9.472
IPC2.peptide.svr19 8.706
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1028
0
1028
397721
47
3317
386.9
43.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.711 ± 0.076
0.718 ± 0.023
5.623 ± 0.073
6.032 ± 0.13
5.345 ± 0.062
4.816 ± 0.082
1.098 ± 0.028
8.972 ± 0.079
9.528 ± 0.119
8.961 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.708 ± 0.031
8.754 ± 0.105
2.566 ± 0.042
2.935 ± 0.038
2.387 ± 0.041
8.474 ± 0.127
6.137 ± 0.117
5.824 ± 0.062
0.958 ± 0.024
4.453 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here