Thermococcus prieurii virus 1
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H6VZB6|H6VZB6_9VIRU IclR family transcription regulator OS=Thermococcus prieurii virus 1 OX=1115696 PE=4 SV=1
MM1 pKa = 8.11 VEE3 pKa = 4.45 VYY5 pKa = 10.31 CHH7 pKa = 6.9 LSAEE11 pKa = 4.26 EE12 pKa = 4.06 AEE14 pKa = 4.8 KK15 pKa = 10.57 IALEE19 pKa = 3.75 IYY21 pKa = 9.85 NRR23 pKa = 11.84 YY24 pKa = 8.49 KK25 pKa = 11.15 AEE27 pKa = 3.85 VDD29 pKa = 3.77 KK30 pKa = 11.19 KK31 pKa = 9.15 WEE33 pKa = 3.94 QFKK36 pKa = 11.3 KK37 pKa = 10.41 EE38 pKa = 4.07 MEE40 pKa = 4.37 GVPPEE45 pKa = 3.8 ILYY48 pKa = 10.09 DD49 pKa = 3.65 QEE51 pKa = 5.2 LWEE54 pKa = 4.79 DD55 pKa = 2.85 WMMDD59 pKa = 2.71 RR60 pKa = 11.84 WIQLVDD66 pKa = 4.31 EE67 pKa = 4.74 IDD69 pKa = 3.57 YY70 pKa = 11.35 LSILEE75 pKa = 4.28 EE76 pKa = 3.88 YY77 pKa = 9.43 RR78 pKa = 11.84 KK79 pKa = 10.23 RR80 pKa = 11.84 GISVFLEE87 pKa = 3.99 HH88 pKa = 7.14 EE89 pKa = 3.68 EE90 pKa = 4.38 DD91 pKa = 3.07 IRR93 pKa = 11.84 VVVEE97 pKa = 3.67 SSGFRR102 pKa = 11.84 IEE104 pKa = 4.37 VVNGEE109 pKa = 4.68 PNFEE113 pKa = 4.02 WGGFRR118 pKa = 11.84 SWKK121 pKa = 10.28 YY122 pKa = 9.81 SPNWNSARR130 pKa = 11.84 RR131 pKa = 11.84 IDD133 pKa = 5.76 LLRR136 pKa = 11.84 AWMGFARR143 pKa = 11.84 LFYY146 pKa = 10.52 PEE148 pKa = 4.52 GFDD151 pKa = 5.06 RR152 pKa = 11.84 GAFDD156 pKa = 3.86 HH157 pKa = 7.26 DD158 pKa = 3.59 LWLEE162 pKa = 4.16 LEE164 pKa = 4.95 VYY166 pKa = 10.01 WDD168 pKa = 3.37 MWANGAWKK176 pKa = 10.25 EE177 pKa = 4.19 GGGNSLWDD185 pKa = 3.53 VAA187 pKa = 4.87
Molecular weight: 22.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.567
IPC2_protein 4.533
IPC_protein 4.444
Toseland 4.304
ProMoST 4.546
Dawson 4.38
Bjellqvist 4.533
Wikipedia 4.228
Rodwell 4.291
Grimsley 4.215
Solomon 4.38
Lehninger 4.329
Nozaki 4.482
DTASelect 4.609
Thurlkill 4.291
EMBOSS 4.253
Sillero 4.558
Patrickios 3.834
IPC_peptide 4.393
IPC2_peptide 4.546
IPC2.peptide.svr19 4.499
Protein with the highest isoelectric point:
>tr|H6VZC4|H6VZC4_9VIRU Putative integral membrane protein OS=Thermococcus prieurii virus 1 OX=1115696 PE=4 SV=1
MM1 pKa = 7.45 GLLLEE6 pKa = 4.26 VLRR9 pKa = 11.84 RR10 pKa = 11.84 AWRR13 pKa = 11.84 DD14 pKa = 2.85 GFEE17 pKa = 4.11 RR18 pKa = 11.84 GKK20 pKa = 10.48 KK21 pKa = 9.57 LAMKK25 pKa = 10.15 RR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 GSLDD33 pKa = 3.04 PRR35 pKa = 11.84 IGMALAATFIGGFIALLVVAQVQPQVDD62 pKa = 3.44 QSIPANSTLYY72 pKa = 10.29 TSYY75 pKa = 9.28 QTFTGYY81 pKa = 9.9 VGKK84 pKa = 10.37 SFSMWGLAIFIGVLGMVVGAIYY106 pKa = 10.44 GFLGRR111 pKa = 11.84 GG112 pKa = 3.26
Molecular weight: 12.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 10.101
IPC_protein 11.096
Toseland 11.038
ProMoST 11.111
Dawson 11.111
Bjellqvist 10.95
Wikipedia 11.433
Rodwell 11.155
Grimsley 11.169
Solomon 11.374
Lehninger 11.316
Nozaki 11.008
DTASelect 10.95
Thurlkill 11.038
EMBOSS 11.477
Sillero 11.067
Patrickios 10.906
IPC_peptide 11.374
IPC2_peptide 10.145
IPC2.peptide.svr19 8.303
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28
0
28
6392
62
884
228.3
25.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.15 ± 0.327
0.407 ± 0.123
4.443 ± 0.265
7.65 ± 0.887
4.506 ± 0.283
7.275 ± 0.566
1.486 ± 0.187
6.054 ± 0.341
7.056 ± 0.826
9.903 ± 0.518
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.159 ± 0.238
3.88 ± 0.427
4.881 ± 0.626
3.16 ± 0.278
5.695 ± 0.752
6.399 ± 0.678
5.116 ± 0.72
7.462 ± 0.519
1.752 ± 0.236
3.567 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here