Thermococcus prieurii virus 1

Taxonomy: Viruses; unclassified archaeal viruses

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|H6VZB6|H6VZB6_9VIRU IclR family transcription regulator OS=Thermococcus prieurii virus 1 OX=1115696 PE=4 SV=1
MM1 pKa = 8.11VEE3 pKa = 4.45VYY5 pKa = 10.31CHH7 pKa = 6.9LSAEE11 pKa = 4.26EE12 pKa = 4.06AEE14 pKa = 4.8KK15 pKa = 10.57IALEE19 pKa = 3.75IYY21 pKa = 9.85NRR23 pKa = 11.84YY24 pKa = 8.49KK25 pKa = 11.15AEE27 pKa = 3.85VDD29 pKa = 3.77KK30 pKa = 11.19KK31 pKa = 9.15WEE33 pKa = 3.94QFKK36 pKa = 11.3KK37 pKa = 10.41EE38 pKa = 4.07MEE40 pKa = 4.37GVPPEE45 pKa = 3.8ILYY48 pKa = 10.09DD49 pKa = 3.65QEE51 pKa = 5.2LWEE54 pKa = 4.79DD55 pKa = 2.85WMMDD59 pKa = 2.71RR60 pKa = 11.84WIQLVDD66 pKa = 4.31EE67 pKa = 4.74IDD69 pKa = 3.57YY70 pKa = 11.35LSILEE75 pKa = 4.28EE76 pKa = 3.88YY77 pKa = 9.43RR78 pKa = 11.84KK79 pKa = 10.23RR80 pKa = 11.84GISVFLEE87 pKa = 3.99HH88 pKa = 7.14EE89 pKa = 3.68EE90 pKa = 4.38DD91 pKa = 3.07IRR93 pKa = 11.84VVVEE97 pKa = 3.67SSGFRR102 pKa = 11.84IEE104 pKa = 4.37VVNGEE109 pKa = 4.68PNFEE113 pKa = 4.02WGGFRR118 pKa = 11.84SWKK121 pKa = 10.28YY122 pKa = 9.81SPNWNSARR130 pKa = 11.84RR131 pKa = 11.84IDD133 pKa = 5.76LLRR136 pKa = 11.84AWMGFARR143 pKa = 11.84LFYY146 pKa = 10.52PEE148 pKa = 4.52GFDD151 pKa = 5.06RR152 pKa = 11.84GAFDD156 pKa = 3.86HH157 pKa = 7.26DD158 pKa = 3.59LWLEE162 pKa = 4.16LEE164 pKa = 4.95VYY166 pKa = 10.01WDD168 pKa = 3.37MWANGAWKK176 pKa = 10.25EE177 pKa = 4.19GGGNSLWDD185 pKa = 3.53VAA187 pKa = 4.87

Molecular weight:
22.51 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|H6VZC4|H6VZC4_9VIRU Putative integral membrane protein OS=Thermococcus prieurii virus 1 OX=1115696 PE=4 SV=1
MM1 pKa = 7.45GLLLEE6 pKa = 4.26VLRR9 pKa = 11.84RR10 pKa = 11.84AWRR13 pKa = 11.84DD14 pKa = 2.85GFEE17 pKa = 4.11RR18 pKa = 11.84GKK20 pKa = 10.48KK21 pKa = 9.57LAMKK25 pKa = 10.15RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84GSLDD33 pKa = 3.04PRR35 pKa = 11.84IGMALAATFIGGFIALLVVAQVQPQVDD62 pKa = 3.44QSIPANSTLYY72 pKa = 10.29TSYY75 pKa = 9.28QTFTGYY81 pKa = 9.9VGKK84 pKa = 10.37SFSMWGLAIFIGVLGMVVGAIYY106 pKa = 10.44GFLGRR111 pKa = 11.84GG112 pKa = 3.26

Molecular weight:
12.26 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28

0

28

6392

62

884

228.3

25.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.15 ± 0.327

0.407 ± 0.123

4.443 ± 0.265

7.65 ± 0.887

4.506 ± 0.283

7.275 ± 0.566

1.486 ± 0.187

6.054 ± 0.341

7.056 ± 0.826

9.903 ± 0.518

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.159 ± 0.238

3.88 ± 0.427

4.881 ± 0.626

3.16 ± 0.278

5.695 ± 0.752

6.399 ± 0.678

5.116 ± 0.72

7.462 ± 0.519

1.752 ± 0.236

3.567 ± 0.313

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski