Suid alphaherpesvirus 1
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5PP75|Q5PP75_9ALPH Immediate-early protein IE180 OS=Suid alphaherpesvirus 1 OX=10345 GN=IE180(TRS) PE=3 SV=1
MM1 pKa = 7.59 DD2 pKa = 5.22 RR3 pKa = 11.84 VWADD7 pKa = 2.66 WYY9 pKa = 10.38 EE10 pKa = 4.25 PVPSPPFSPVDD21 pKa = 3.55 PPGPRR26 pKa = 11.84 PTTPVPGSSPPSPASTPTPPKK47 pKa = 8.29 RR48 pKa = 11.84 GRR50 pKa = 11.84 YY51 pKa = 7.89 VVEE54 pKa = 4.11 HH55 pKa = 7.06 PEE57 pKa = 4.1 YY58 pKa = 10.82 GPPPDD63 pKa = 3.94 PEE65 pKa = 4.09 EE66 pKa = 4.2 VRR68 pKa = 11.84 VHH70 pKa = 6.28 GARR73 pKa = 11.84 GPGAFCAAPWRR84 pKa = 11.84 PDD86 pKa = 3.04 TRR88 pKa = 11.84 RR89 pKa = 11.84 LGADD93 pKa = 3.12 VNRR96 pKa = 11.84 LFRR99 pKa = 11.84 GIAVSAADD107 pKa = 3.48 VTGDD111 pKa = 3.15 TRR113 pKa = 11.84 ALRR116 pKa = 11.84 RR117 pKa = 11.84 ALFDD121 pKa = 4.26 FYY123 pKa = 11.86 AMGYY127 pKa = 6.58 TRR129 pKa = 11.84 QRR131 pKa = 11.84 PSAPCWQALLQLSPEE146 pKa = 3.76 QSAPLRR152 pKa = 11.84 SALRR156 pKa = 11.84 EE157 pKa = 3.93 LNEE160 pKa = 3.95 RR161 pKa = 11.84 DD162 pKa = 3.66 VYY164 pKa = 10.87 DD165 pKa = 4.16 PRR167 pKa = 11.84 VLSPPVIEE175 pKa = 4.94 GPLFGEE181 pKa = 4.35 EE182 pKa = 4.28 CDD184 pKa = 4.4 VDD186 pKa = 4.21 EE187 pKa = 6.2 DD188 pKa = 5.04 DD189 pKa = 5.38 AGSDD193 pKa = 3.3 TTVASEE199 pKa = 3.76 FSFRR203 pKa = 11.84 GSVCEE208 pKa = 5.61 DD209 pKa = 3.48 DD210 pKa = 6.26 GEE212 pKa = 5.66 DD213 pKa = 3.76 EE214 pKa = 5.57 DD215 pKa = 4.98 EE216 pKa = 4.74 EE217 pKa = 4.59 EE218 pKa = 4.97 DD219 pKa = 5.04 GEE221 pKa = 4.86 EE222 pKa = 3.99 EE223 pKa = 5.22 DD224 pKa = 3.89 EE225 pKa = 4.63 DD226 pKa = 4.38 EE227 pKa = 4.51 EE228 pKa = 5.48 GEE230 pKa = 4.16 EE231 pKa = 4.26 EE232 pKa = 3.96 EE233 pKa = 5.72 DD234 pKa = 3.77 EE235 pKa = 4.33 EE236 pKa = 4.92 EE237 pKa = 4.78 EE238 pKa = 4.62 EE239 pKa = 4.39 GDD241 pKa = 3.53 EE242 pKa = 5.3 DD243 pKa = 5.58 GEE245 pKa = 4.35 TDD247 pKa = 3.84 VYY249 pKa = 11.45 EE250 pKa = 4.72 EE251 pKa = 4.71 DD252 pKa = 5.59 DD253 pKa = 3.79 EE254 pKa = 7.03 AEE256 pKa = 4.27 DD257 pKa = 5.23 EE258 pKa = 4.18 EE259 pKa = 5.7 DD260 pKa = 5.22 EE261 pKa = 4.88 EE262 pKa = 6.68 DD263 pKa = 5.17 GDD265 pKa = 4.55 DD266 pKa = 4.41 FDD268 pKa = 4.74 GASVGDD274 pKa = 4.22 DD275 pKa = 3.59 DD276 pKa = 5.72 VFEE279 pKa = 4.61 PPEE282 pKa = 4.86 DD283 pKa = 4.11 GSDD286 pKa = 3.99 GEE288 pKa = 5.04 GSGSDD293 pKa = 3.8 DD294 pKa = 4.01 GGDD297 pKa = 4.28 GEE299 pKa = 6.4 DD300 pKa = 3.72 EE301 pKa = 5.91 DD302 pKa = 4.83 EE303 pKa = 6.67 DD304 pKa = 4.12 EE305 pKa = 6.69 DD306 pKa = 4.69 EE307 pKa = 6.73 DD308 pKa = 4.48 EE309 pKa = 6.34 DD310 pKa = 4.7 EE311 pKa = 6.26 DD312 pKa = 5.53 DD313 pKa = 6.14 GEE315 pKa = 4.74 DD316 pKa = 3.7 EE317 pKa = 4.2 EE318 pKa = 6.18 DD319 pKa = 4.28 EE320 pKa = 4.28 EE321 pKa = 6.54 GEE323 pKa = 4.64 DD324 pKa = 3.99 GGEE327 pKa = 4.13 DD328 pKa = 3.96 GEE330 pKa = 5.6 DD331 pKa = 3.73 GEE333 pKa = 4.74 EE334 pKa = 5.4 DD335 pKa = 3.54 EE336 pKa = 6.37 DD337 pKa = 4.29 EE338 pKa = 5.0 DD339 pKa = 5.64 GEE341 pKa = 4.51 GEE343 pKa = 4.44 EE344 pKa = 4.69 GGKK347 pKa = 10.07 DD348 pKa = 2.88 AARR351 pKa = 11.84 RR352 pKa = 11.84 GTRR355 pKa = 11.84 APTRR359 pKa = 11.84 PAAAPP364 pKa = 3.5
Molecular weight: 39.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.259
IPC2_protein 3.554
IPC_protein 3.579
Toseland 3.363
ProMoST 3.719
Dawson 3.554
Bjellqvist 3.706
Wikipedia 3.465
Rodwell 3.401
Grimsley 3.274
Solomon 3.554
Lehninger 3.503
Nozaki 3.668
DTASelect 3.859
Thurlkill 3.401
EMBOSS 3.478
Sillero 3.694
Patrickios 3.376
IPC_peptide 3.554
IPC2_peptide 3.681
IPC2.peptide.svr19 3.71
Protein with the highest isoelectric point:
>tr|G3G8W3|G3G8W3_9ALPH DNA primase OS=Suid alphaherpesvirus 1 OX=10345 GN=UL52 PE=3 SV=1
MM1 pKa = 7.81 EE2 pKa = 6.23 DD3 pKa = 3.58 SGNSSGSEE11 pKa = 3.65 ASRR14 pKa = 11.84 SGSEE18 pKa = 3.41 EE19 pKa = 3.47 RR20 pKa = 11.84 RR21 pKa = 11.84 PVRR24 pKa = 11.84 EE25 pKa = 3.98 RR26 pKa = 11.84 LGSRR30 pKa = 11.84 PPEE33 pKa = 4.01 RR34 pKa = 11.84 RR35 pKa = 11.84 PVRR38 pKa = 11.84 ARR40 pKa = 11.84 LGAIRR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 GGRR51 pKa = 11.84 GGRR54 pKa = 11.84 AARR57 pKa = 11.84 QALRR61 pKa = 11.84 QRR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 QQQQQQRR72 pKa = 11.84 QQQHH76 pKa = 3.04 QRR78 pKa = 11.84 RR79 pKa = 11.84 RR80 pKa = 11.84 QEE82 pKa = 3.26 ADD84 pKa = 3.57 RR85 pKa = 11.84 PDD87 pKa = 4.37 GGPDD91 pKa = 3.32 APPDD95 pKa = 3.74 RR96 pKa = 11.84 LSEE99 pKa = 4.18 SARR102 pKa = 11.84 AAVSATHH109 pKa = 6.17 ARR111 pKa = 11.84 VGATRR116 pKa = 11.84 VNEE119 pKa = 4.05 LFASARR125 pKa = 11.84 HH126 pKa = 5.78 DD127 pKa = 3.5 LSRR130 pKa = 11.84 PVFNDD135 pKa = 3.39 GFRR138 pKa = 11.84 AAGSSPWAAVLEE150 pKa = 4.87 FGAEE154 pKa = 3.86 QFTPDD159 pKa = 3.16 GRR161 pKa = 11.84 RR162 pKa = 11.84 VTWEE166 pKa = 3.44 TLMFHH171 pKa = 6.9 GADD174 pKa = 3.25 LHH176 pKa = 6.84 RR177 pKa = 11.84 LFEE180 pKa = 4.36 VRR182 pKa = 11.84 PHH184 pKa = 5.21 ATEE187 pKa = 3.54 AARR190 pKa = 11.84 VLRR193 pKa = 11.84 EE194 pKa = 3.8 MVLLNEE200 pKa = 4.7 GLTEE204 pKa = 4.17 SLASADD210 pKa = 4.06 EE211 pKa = 4.03 TLTWVKK217 pKa = 10.87 LILTKK222 pKa = 10.87 GLTLRR227 pKa = 11.84 TLDD230 pKa = 4.98 PIVATAGAVLQNLRR244 pKa = 11.84 LKK246 pKa = 10.67 LGPFLRR252 pKa = 11.84 CYY254 pKa = 10.85 LRR256 pKa = 11.84 DD257 pKa = 3.65 TPVDD261 pKa = 3.25 EE262 pKa = 4.21 LVRR265 pKa = 11.84 RR266 pKa = 11.84 RR267 pKa = 11.84 RR268 pKa = 11.84 LRR270 pKa = 11.84 DD271 pKa = 3.01 VRR273 pKa = 11.84 CIVTYY278 pKa = 8.89 TLVMLARR285 pKa = 11.84 IARR288 pKa = 11.84 VVEE291 pKa = 4.35 RR292 pKa = 11.84 GSSCVLPEE300 pKa = 4.69 DD301 pKa = 5.18 LGDD304 pKa = 3.79 SPVPLEE310 pKa = 4.14 EE311 pKa = 4.26 YY312 pKa = 11.14 VPGACLGGIMDD323 pKa = 5.53 ALDD326 pKa = 3.55 SHH328 pKa = 6.5 KK329 pKa = 9.4 TGCDD333 pKa = 3.09 APTCRR338 pKa = 11.84 LTCSYY343 pKa = 10.28 TLVPVYY349 pKa = 9.35 MHH351 pKa = 6.9 GKK353 pKa = 9.54 YY354 pKa = 10.31 FYY356 pKa = 10.45 CNHH359 pKa = 6.76 LFF361 pKa = 3.62
Molecular weight: 40.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.224
IPC_protein 10.189
Toseland 10.043
ProMoST 9.97
Dawson 10.277
Bjellqvist 10.131
Wikipedia 10.555
Rodwell 10.233
Grimsley 10.365
Solomon 10.394
Lehninger 10.335
Nozaki 10.204
DTASelect 10.087
Thurlkill 10.145
EMBOSS 10.482
Sillero 10.248
Patrickios 9.443
IPC_peptide 10.379
IPC2_peptide 9.575
IPC2.peptide.svr19 8.375
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
69
2
71
35751
63
3084
503.5
53.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.614 ± 0.533
1.589 ± 0.121
5.645 ± 0.203
5.77 ± 0.232
3.217 ± 0.202
8.492 ± 0.772
2.447 ± 0.155
1.885 ± 0.144
1.236 ± 0.097
9.818 ± 0.289
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.592 ± 0.126
1.698 ± 0.152
8.341 ± 0.546
2.45 ± 0.143
9.656 ± 0.221
5.046 ± 0.294
4.696 ± 0.211
7.544 ± 0.256
0.962 ± 0.066
2.302 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here