Hubei odonate virus 6
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KKX6|A0A1L3KKX6_9VIRU Uncharacterized protein OS=Hubei odonate virus 6 OX=1923001 PE=4 SV=1
MM1 pKa = 7.99 DD2 pKa = 5.56 LLAEE6 pKa = 4.01 VWEE9 pKa = 4.54 RR10 pKa = 11.84 DD11 pKa = 3.37 TTLVDD16 pKa = 3.47 VLDD19 pKa = 4.61 DD20 pKa = 3.96 SGIGEE25 pKa = 4.19 IPSIQSKK32 pKa = 10.4 INAIQEE38 pKa = 4.14 EE39 pKa = 4.39 QSQQKK44 pKa = 10.23 DD45 pKa = 3.34 QLDD48 pKa = 4.28 SLGHH52 pKa = 5.44 TVDD55 pKa = 4.54 NNQKK59 pKa = 10.21 SNEE62 pKa = 3.71 ARR64 pKa = 11.84 ILDD67 pKa = 4.64 LYY69 pKa = 10.93 TNVNSNTRR77 pKa = 11.84 DD78 pKa = 3.54 LQRR81 pKa = 11.84 LEE83 pKa = 4.67 QEE85 pKa = 4.57 VKK87 pKa = 10.83 DD88 pKa = 3.79 LTLSIGKK95 pKa = 8.54 IQLQMHH101 pKa = 5.93 EE102 pKa = 5.02 LEE104 pKa = 5.07 LKK106 pKa = 10.58 VSSNSQIIDD115 pKa = 3.4 DD116 pKa = 3.9 MTYY119 pKa = 10.95 DD120 pKa = 3.78 LEE122 pKa = 4.54 VVKK125 pKa = 11.16 GNVLTLAQDD134 pKa = 3.52 MNKK137 pKa = 10.28 LNNEE141 pKa = 4.12 LLLVKK146 pKa = 10.24 PDD148 pKa = 3.39 VSEE151 pKa = 4.95 LKK153 pKa = 9.91 SQNVFTYY160 pKa = 9.78 PFSVYY165 pKa = 11.19 ANIFLQDD172 pKa = 2.82 ICAVYY177 pKa = 10.08 EE178 pKa = 4.28 GSKK181 pKa = 8.68 VTIVDD186 pKa = 3.07 IGGSSIEE193 pKa = 4.18 YY194 pKa = 7.66 QTTTIGSGMGRR205 pKa = 11.84 KK206 pKa = 9.12 VSSPVKK212 pKa = 8.84 YY213 pKa = 9.34 VKK215 pKa = 10.19 RR216 pKa = 11.84 YY217 pKa = 8.27 EE218 pKa = 4.09 LVPSVVFNKK227 pKa = 10.02 KK228 pKa = 8.15 VYY230 pKa = 10.28 QYY232 pKa = 11.11 ISFVGINRR240 pKa = 11.84 TTSQPAFSDD249 pKa = 3.83 DD250 pKa = 4.88 PIVTWGYY257 pKa = 9.37 QVIKK261 pKa = 10.63 LSNNVNLDD269 pKa = 3.01 AMSIMLIKK277 pKa = 9.98 EE278 pKa = 3.8 AYY280 pKa = 9.87 VPVNFSKK287 pKa = 10.69 IKK289 pKa = 10.33
Molecular weight: 32.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.728
IPC2_protein 4.724
IPC_protein 4.647
Toseland 4.495
ProMoST 4.774
Dawson 4.609
Bjellqvist 4.762
Wikipedia 4.495
Rodwell 4.495
Grimsley 4.393
Solomon 4.609
Lehninger 4.558
Nozaki 4.711
DTASelect 4.902
Thurlkill 4.495
EMBOSS 4.507
Sillero 4.762
Patrickios 3.999
IPC_peptide 4.609
IPC2_peptide 4.762
IPC2.peptide.svr19 4.718
Protein with the highest isoelectric point:
>tr|A0A1L3KKV8|A0A1L3KKV8_9VIRU Uncharacterized protein OS=Hubei odonate virus 6 OX=1923001 PE=4 SV=1
MM1 pKa = 6.98 TTRR4 pKa = 11.84 KK5 pKa = 9.9 CDD7 pKa = 4.4 DD8 pKa = 3.51 ILRR11 pKa = 11.84 KK12 pKa = 9.37 EE13 pKa = 4.27 PNYY16 pKa = 10.58 SRR18 pKa = 11.84 QNTTLANDD26 pKa = 3.26 INIDD30 pKa = 3.46 LKK32 pKa = 10.25 RR33 pKa = 11.84 RR34 pKa = 11.84 KK35 pKa = 9.84 RR36 pKa = 11.84 ISPYY40 pKa = 10.67 LLDD43 pKa = 3.92 MLNEE47 pKa = 4.2 PVISDD52 pKa = 4.31 KK53 pKa = 10.72 PVKK56 pKa = 8.91 KK57 pKa = 10.53 CKK59 pKa = 7.95 VARR62 pKa = 11.84 MTGHH66 pKa = 5.39 TTRR69 pKa = 11.84 KK70 pKa = 9.54 YY71 pKa = 8.61 STKK74 pKa = 10.13 TRR76 pKa = 11.84 DD77 pKa = 3.64 LNSKK81 pKa = 6.71 SHH83 pKa = 6.55 KK84 pKa = 10.4 VMTVTRR90 pKa = 11.84 PEE92 pKa = 3.81 NRR94 pKa = 11.84 QVTVIQKK101 pKa = 9.69 VEE103 pKa = 4.02 EE104 pKa = 4.17 VQPKK108 pKa = 8.15 LTCKK112 pKa = 9.07 QRR114 pKa = 11.84 RR115 pKa = 11.84 KK116 pKa = 9.07 MRR118 pKa = 11.84 SSLRR122 pKa = 11.84 ICLYY126 pKa = 10.92 NKK128 pKa = 9.8 ANKK131 pKa = 10.16 GKK133 pKa = 8.0 TLLEE137 pKa = 4.04 NKK139 pKa = 8.63 TWVEE143 pKa = 4.26 KK144 pKa = 10.57 IAISDD149 pKa = 3.49 RR150 pKa = 11.84 DD151 pKa = 3.74 QVPEE155 pKa = 5.48 AIQQCYY161 pKa = 9.5 IPPPRR166 pKa = 11.84 KK167 pKa = 9.17 QSYY170 pKa = 8.65 EE171 pKa = 4.06 RR172 pKa = 11.84 IVPGRR177 pKa = 11.84 VNLNTIDD184 pKa = 4.66 YY185 pKa = 7.76 EE186 pKa = 4.65 VHH188 pKa = 7.32 DD189 pKa = 4.43 NWTKK193 pKa = 11.06 LSDD196 pKa = 3.36 QDD198 pKa = 3.97 VGVKK202 pKa = 9.76 FSKK205 pKa = 10.66 RR206 pKa = 11.84 MTHH209 pKa = 6.61 NSDD212 pKa = 3.14 EE213 pKa = 4.31 NALSKK218 pKa = 10.92 LFAEE222 pKa = 4.32 IRR224 pKa = 11.84 KK225 pKa = 8.78 EE226 pKa = 3.91 FSEE229 pKa = 4.53 TEE231 pKa = 3.94 LEE233 pKa = 4.19 RR234 pKa = 11.84 KK235 pKa = 9.54 VRR237 pKa = 11.84 EE238 pKa = 3.81 QVKK241 pKa = 10.6 DD242 pKa = 3.48 VTQTLLSTQNATVDD256 pKa = 3.59 RR257 pKa = 11.84 LIKK260 pKa = 10.26 EE261 pKa = 4.2 VVALRR266 pKa = 11.84 EE267 pKa = 3.92 EE268 pKa = 4.42 LNRR271 pKa = 11.84 ANAEE275 pKa = 4.11 VASLKK280 pKa = 10.53 RR281 pKa = 11.84 KK282 pKa = 10.28 LEE284 pKa = 4.14 VIPEE288 pKa = 4.02 VEE290 pKa = 4.33 QQCSVVVPSTTIPTRR305 pKa = 11.84 LLPLKK310 pKa = 10.19 KK311 pKa = 10.25 RR312 pKa = 11.84 IIAEE316 pKa = 3.85 VSKK319 pKa = 11.28 ADD321 pKa = 3.5 TTIVSEE327 pKa = 4.25 PEE329 pKa = 3.79 KK330 pKa = 10.76 VNPVIKK336 pKa = 10.43 KK337 pKa = 6.63 VTPKK341 pKa = 10.75 VIVSPKK347 pKa = 9.63 IDD349 pKa = 3.09 VKK351 pKa = 11.61 ANVQIDD357 pKa = 3.86 DD358 pKa = 4.62 EE359 pKa = 4.81 KK360 pKa = 11.24 PSTSKK365 pKa = 10.51 QALIKK370 pKa = 9.76 TNSEE374 pKa = 4.09 VASEE378 pKa = 3.94 IAKK381 pKa = 8.76 VQKK384 pKa = 10.14 PSEE387 pKa = 3.96 IVIKK391 pKa = 10.57 QKK393 pKa = 11.12 SDD395 pKa = 3.16 LTFEE399 pKa = 4.3 SRR401 pKa = 11.84 VIPKK405 pKa = 10.02 IKK407 pKa = 9.2 EE408 pKa = 4.15 TYY410 pKa = 8.01 TDD412 pKa = 3.65 RR413 pKa = 11.84 DD414 pKa = 2.76 IDD416 pKa = 3.41 MWIDD420 pKa = 3.62 FNLKK424 pKa = 10.42 NEE426 pKa = 4.18 DD427 pKa = 3.44 VRR429 pKa = 11.84 KK430 pKa = 10.35 KK431 pKa = 10.66 EE432 pKa = 4.01 ILDD435 pKa = 3.77 SLIEE439 pKa = 4.48 DD440 pKa = 4.15 DD441 pKa = 5.8 KK442 pKa = 12.0 SFASLFDD449 pKa = 3.4 KK450 pKa = 10.73 HH451 pKa = 6.86 ASPTQRR457 pKa = 11.84 LNFAKK462 pKa = 10.3 LGCEE466 pKa = 3.95 SSRR469 pKa = 11.84 ARR471 pKa = 11.84 INAQDD476 pKa = 3.38 INRR479 pKa = 11.84 FTWTKK484 pKa = 9.92 ICAKK488 pKa = 9.84 HH489 pKa = 5.45 KK490 pKa = 9.4 TDD492 pKa = 3.61 AEE494 pKa = 4.2 RR495 pKa = 11.84 KK496 pKa = 8.96 VEE498 pKa = 3.96 CDD500 pKa = 3.48 KK501 pKa = 11.14 IRR503 pKa = 11.84 ATLFRR508 pKa = 11.84 TLFFRR513 pKa = 11.84 KK514 pKa = 8.88 QAEE517 pKa = 4.35 FKK519 pKa = 10.54 KK520 pKa = 10.95 LNLGVVNPCLIGVNRR535 pKa = 11.84 WITKK539 pKa = 9.84 LLSLKK544 pKa = 9.16 TAEE547 pKa = 3.57 EE548 pKa = 4.17 RR549 pKa = 11.84 IYY551 pKa = 10.98 FRR553 pKa = 11.84 TSWNNKK559 pKa = 7.29 VDD561 pKa = 4.47 AITIRR566 pKa = 11.84 LQNLAKK572 pKa = 10.24 QKK574 pKa = 10.7 CC575 pKa = 3.45
Molecular weight: 66.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.924
IPC2_protein 8.96
IPC_protein 8.829
Toseland 9.999
ProMoST 9.487
Dawson 10.116
Bjellqvist 9.706
Wikipedia 10.204
Rodwell 10.76
Grimsley 10.131
Solomon 10.131
Lehninger 10.116
Nozaki 10.028
DTASelect 9.692
Thurlkill 9.999
EMBOSS 10.379
Sillero 10.028
Patrickios 10.438
IPC_peptide 10.131
IPC2_peptide 8.185
IPC2.peptide.svr19 7.881
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3826
289
1991
956.5
108.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.829 ± 0.55
1.464 ± 0.194
5.567 ± 0.395
6.325 ± 0.307
3.999 ± 0.533
5.018 ± 0.99
1.777 ± 0.5
6.926 ± 0.438
7.188 ± 1.33
9.2 ± 0.829
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.934 ± 0.164
5.227 ± 0.384
4.339 ± 0.926
4.182 ± 0.392
4.861 ± 0.732
7.371 ± 0.317
6.717 ± 0.656
7.527 ± 0.566
1.176 ± 0.138
3.372 ± 0.488
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here