Clostridium phage HM T
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L2BY98|A0A1L2BY98_9CAUD Head-tail adaptor OS=Clostridium phage HM T OX=1779843 GN=HMT_31 PE=4 SV=1
MM1 pKa = 8.11 DD2 pKa = 5.97 IIEE5 pKa = 5.34 KK6 pKa = 10.05 IDD8 pKa = 3.44 IALSNALDD16 pKa = 4.01 PLNIRR21 pKa = 11.84 SFYY24 pKa = 10.5 GWYY27 pKa = 10.23 DD28 pKa = 3.28 EE29 pKa = 5.94 DD30 pKa = 4.07 INGTHH35 pKa = 5.3 VTFTLISDD43 pKa = 4.37 LDD45 pKa = 3.55 EE46 pKa = 6.48 DD47 pKa = 4.4 YY48 pKa = 11.71 ADD50 pKa = 5.55 DD51 pKa = 4.02 EE52 pKa = 4.99 AEE54 pKa = 4.17 STTTLFQVDD63 pKa = 3.05 IWSKK67 pKa = 11.35 EE68 pKa = 3.64 NMEE71 pKa = 4.34 EE72 pKa = 3.89 LKK74 pKa = 9.69 KK75 pKa = 9.4 TIKK78 pKa = 10.51 SAMKK82 pKa = 9.64 TLDD85 pKa = 3.15 NCTYY89 pKa = 11.16 NSGADD94 pKa = 3.49 LYY96 pKa = 11.47 EE97 pKa = 4.78 NDD99 pKa = 3.13 TKK101 pKa = 10.6 IYY103 pKa = 10.53 HH104 pKa = 6.18 KK105 pKa = 10.57 ALRR108 pKa = 11.84 FNITQEE114 pKa = 4.09 VTT116 pKa = 3.02
Molecular weight: 13.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.218
IPC2_protein 4.139
IPC_protein 4.088
Toseland 3.897
ProMoST 4.24
Dawson 4.075
Bjellqvist 4.228
Wikipedia 3.986
Rodwell 3.923
Grimsley 3.808
Solomon 4.062
Lehninger 4.024
Nozaki 4.19
DTASelect 4.406
Thurlkill 3.935
EMBOSS 3.999
Sillero 4.215
Patrickios 3.478
IPC_peptide 4.062
IPC2_peptide 4.19
IPC2.peptide.svr19 4.13
Protein with the highest isoelectric point:
>tr|A0A1L2BY88|A0A1L2BY88_9CAUD Uncharacterized protein OS=Clostridium phage HM T OX=1779843 GN=HMT_21 PE=4 SV=1
MM1 pKa = 7.28 GRR3 pKa = 11.84 KK4 pKa = 9.0 RR5 pKa = 11.84 PAKK8 pKa = 9.79 PIVRR12 pKa = 11.84 TDD14 pKa = 3.34 QVLDD18 pKa = 3.22 IQDD21 pKa = 3.48 YY22 pKa = 10.6 LKK24 pKa = 10.78 YY25 pKa = 10.43 RR26 pKa = 11.84 NEE28 pKa = 3.56 RR29 pKa = 11.84 DD30 pKa = 3.38 YY31 pKa = 11.48 ILFVVGITTGYY42 pKa = 9.91 RR43 pKa = 11.84 AGDD46 pKa = 3.91 LVTLKK51 pKa = 10.87 ARR53 pKa = 11.84 DD54 pKa = 3.26 IRR56 pKa = 11.84 EE57 pKa = 4.05 ALKK60 pKa = 10.69 KK61 pKa = 10.6 SEE63 pKa = 3.83 FTIFEE68 pKa = 4.32 GKK70 pKa = 9.8 KK71 pKa = 10.15 KK72 pKa = 9.72 NSKK75 pKa = 10.3 NIRR78 pKa = 11.84 EE79 pKa = 4.39 KK80 pKa = 10.28 NRR82 pKa = 11.84 KK83 pKa = 7.61 PRR85 pKa = 11.84 TVEE88 pKa = 4.09 IIPKK92 pKa = 8.94 VAKK95 pKa = 10.1 LLKK98 pKa = 10.29 EE99 pKa = 4.08 YY100 pKa = 10.46 IRR102 pKa = 11.84 DD103 pKa = 3.73 KK104 pKa = 11.14 KK105 pKa = 10.59 DD106 pKa = 3.06 YY107 pKa = 10.59 EE108 pKa = 4.55 YY109 pKa = 10.65 IFQSRR114 pKa = 11.84 KK115 pKa = 9.7 GVNKK119 pKa = 10.24 HH120 pKa = 6.04 IGVQAVSNILKK131 pKa = 10.17 EE132 pKa = 3.97 AGEE135 pKa = 4.28 YY136 pKa = 10.29 FGLYY140 pKa = 10.02 DD141 pKa = 4.16 ISAHH145 pKa = 6.0 SMRR148 pKa = 11.84 KK149 pKa = 6.96 TYY151 pKa = 10.33 AYY153 pKa = 9.51 KK154 pKa = 10.17 IYY156 pKa = 10.3 IEE158 pKa = 4.09 SDD160 pKa = 2.78 KK161 pKa = 11.39 DD162 pKa = 3.19 IVAVQKK168 pKa = 11.1 LLGHH172 pKa = 6.56 RR173 pKa = 11.84 TTQEE177 pKa = 3.62 TEE179 pKa = 4.05 LYY181 pKa = 10.23 IGLDD185 pKa = 3.17 KK186 pKa = 11.01 EE187 pKa = 5.31 KK188 pKa = 9.94 YY189 pKa = 7.96 HH190 pKa = 6.49 QYY192 pKa = 10.95 SKK194 pKa = 11.12 SLSDD198 pKa = 3.62 FVRR201 pKa = 4.72
Molecular weight: 23.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.087
IPC2_protein 9.238
IPC_protein 9.151
Toseland 9.853
ProMoST 9.604
Dawson 10.087
Bjellqvist 9.736
Wikipedia 10.248
Rodwell 10.511
Grimsley 10.16
Solomon 10.101
Lehninger 10.058
Nozaki 9.809
DTASelect 9.736
Thurlkill 9.926
EMBOSS 10.262
Sillero 9.984
Patrickios 9.56
IPC_peptide 10.101
IPC2_peptide 8.2
IPC2.peptide.svr19 8.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
11199
42
1069
233.3
26.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.179 ± 0.495
1.072 ± 0.176
6.635 ± 0.295
7.572 ± 0.496
3.965 ± 0.249
5.733 ± 0.45
0.911 ± 0.128
9.09 ± 0.376
9.287 ± 0.497
8.153 ± 0.27
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.402 ± 0.165
8.144 ± 0.325
2.116 ± 0.193
3.018 ± 0.227
3.098 ± 0.393
6.76 ± 0.368
5.858 ± 0.534
5.474 ± 0.336
1.063 ± 0.184
4.474 ± 0.407
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here