Capsicum chinense (Scotch bonnet) (Bonnet pepper)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Solanoideae; Capsiceae; Capsicum

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34757 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G3BAY7|A0A2G3BAY7_CAPCH Receptor-like serine/threonine-protein kinase OS=Capsicum chinense OX=80379 GN=BC332_28891 PE=3 SV=1
MM1 pKa = 7.43SRR3 pKa = 11.84TFWGVASSIGASFDD17 pKa = 3.31EE18 pKa = 5.05CDD20 pKa = 3.33VEE22 pKa = 6.33RR23 pKa = 11.84IDD25 pKa = 5.7GDD27 pKa = 3.66GCEE30 pKa = 4.45KK31 pKa = 10.55SRR33 pKa = 11.84QIGYY37 pKa = 9.47EE38 pKa = 3.98CDD40 pKa = 3.21EE41 pKa = 4.49SRR43 pKa = 11.84PTGDD47 pKa = 3.02GCDD50 pKa = 3.4EE51 pKa = 4.19FRR53 pKa = 11.84PIGDD57 pKa = 4.45GYY59 pKa = 11.31DD60 pKa = 3.45EE61 pKa = 4.86FQPISDD67 pKa = 4.46GCDD70 pKa = 2.81EE71 pKa = 4.27SQQTGDD77 pKa = 3.2GCNDD81 pKa = 2.74SRR83 pKa = 11.84LIGDD87 pKa = 4.14GCDD90 pKa = 3.14VSQPTGDD97 pKa = 3.9GCDD100 pKa = 3.44EE101 pKa = 4.27SQLTRR106 pKa = 11.84DD107 pKa = 4.09GYY109 pKa = 10.27DD110 pKa = 2.99VKK112 pKa = 11.33

Molecular weight: 12.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G3ASV6|A0A2G3ASV6_CAPCH Reverse transcriptase domain-containing protein OS=Capsicum chinense OX=80379 GN=BC332_33756 PE=4 SV=1
MM1 pKa = 7.78LEE3 pKa = 4.37TPPMTNADD11 pKa = 3.56GHH13 pKa = 6.44IVACHH18 pKa = 6.4GGTAAPRR25 pKa = 11.84HH26 pKa = 6.26RR27 pKa = 11.84GTMAPSHH34 pKa = 6.93RR35 pKa = 11.84GTAAPSHH42 pKa = 6.89RR43 pKa = 11.84GTKK46 pKa = 10.19APWHH50 pKa = 6.58HH51 pKa = 6.05RR52 pKa = 11.84TAAPWHH58 pKa = 6.05QGTAARR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84GTMAPRR72 pKa = 11.84HH73 pKa = 5.75QGTVAPTHH81 pKa = 6.14QGTMAPSHH89 pKa = 6.92RR90 pKa = 11.84GTVAPRR96 pKa = 11.84HH97 pKa = 6.35HH98 pKa = 6.28GTAAPRR104 pKa = 11.84HH105 pKa = 6.29RR106 pKa = 11.84GTKK109 pKa = 10.03APSHH113 pKa = 6.85HH114 pKa = 5.96GTIAPRR120 pKa = 11.84HH121 pKa = 5.57QGTAARR127 pKa = 11.84RR128 pKa = 11.84RR129 pKa = 11.84GTMAPRR135 pKa = 11.84HH136 pKa = 6.04HH137 pKa = 6.67GAVAAWRR144 pKa = 11.84HH145 pKa = 4.55GTVAPTHH152 pKa = 6.49KK153 pKa = 10.48GAVAPWRR160 pKa = 11.84RR161 pKa = 11.84GTVAPWHH168 pKa = 6.89HH169 pKa = 6.1GTIAPWRR176 pKa = 11.84RR177 pKa = 11.84GAVAPRR183 pKa = 11.84HH184 pKa = 6.12RR185 pKa = 11.84GTKK188 pKa = 10.34ALTHH192 pKa = 6.25RR193 pKa = 11.84GKK195 pKa = 10.27IKK197 pKa = 10.53RR198 pKa = 11.84AA199 pKa = 3.33

Molecular weight: 21.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34757

0

34757

12923466

28

4980

371.8

41.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.291 ± 0.012

1.888 ± 0.006

5.439 ± 0.009

6.488 ± 0.016

4.26 ± 0.008

6.302 ± 0.014

2.361 ± 0.005

5.681 ± 0.009

6.445 ± 0.013

9.787 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 0.006

4.722 ± 0.009

4.704 ± 0.012

3.563 ± 0.009

5.054 ± 0.01

8.731 ± 0.015

4.897 ± 0.008

6.591 ± 0.009

1.277 ± 0.005

2.998 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski