Amino acid dipepetide frequency for bacterium HR29

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.95AlaAla: 21.95 ± 0.373
1.198AlaCys: 1.198 ± 0.042
5.962AlaAsp: 5.962 ± 0.092
9.614AlaGlu: 9.614 ± 0.136
4.613AlaPhe: 4.613 ± 0.094
11.619AlaGly: 11.619 ± 0.148
2.181AlaHis: 2.181 ± 0.056
6.004AlaIle: 6.004 ± 0.104
2.64AlaLys: 2.64 ± 0.06
14.646AlaLeu: 14.646 ± 0.212
2.826AlaMet: 2.826 ± 0.071
2.451AlaAsn: 2.451 ± 0.068
6.334AlaPro: 6.334 ± 0.122
3.31AlaGln: 3.31 ± 0.069
11.479AlaArg: 11.479 ± 0.136
5.879AlaSer: 5.879 ± 0.11
5.582AlaThr: 5.582 ± 0.09
11.45AlaVal: 11.45 ± 0.174
2.087AlaTrp: 2.087 ± 0.06
2.665AlaTyr: 2.665 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
0.873CysAla: 0.873 ± 0.039
0.095CysCys: 0.095 ± 0.012
0.513CysAsp: 0.513 ± 0.032
0.626CysGlu: 0.626 ± 0.031
0.275CysPhe: 0.275 ± 0.018
1.095CysGly: 1.095 ± 0.044
0.185CysHis: 0.185 ± 0.015
0.363CysIle: 0.363 ± 0.022
0.132CysLys: 0.132 ± 0.014
0.717CysLeu: 0.717 ± 0.032
0.138CysMet: 0.138 ± 0.016
0.184CysAsn: 0.184 ± 0.018
0.559CysPro: 0.559 ± 0.028
0.217CysGln: 0.217 ± 0.018
0.753CysArg: 0.753 ± 0.037
0.477CysSer: 0.477 ± 0.024
0.449CysThr: 0.449 ± 0.03
0.582CysVal: 0.582 ± 0.03
0.122CysTrp: 0.122 ± 0.015
0.256CysTyr: 0.256 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
6.028AspAla: 6.028 ± 0.098
0.374AspCys: 0.374 ± 0.025
2.591AspAsp: 2.591 ± 0.058
3.804AspGlu: 3.804 ± 0.081
1.696AspPhe: 1.696 ± 0.04
4.371AspGly: 4.371 ± 0.09
0.945AspHis: 0.945 ± 0.034
2.33AspIle: 2.33 ± 0.057
0.619AspLys: 0.619 ± 0.028
5.299AspLeu: 5.299 ± 0.087
0.719AspMet: 0.719 ± 0.033
0.628AspAsn: 0.628 ± 0.035
4.111AspPro: 4.111 ± 0.073
1.108AspGln: 1.108 ± 0.047
4.07AspArg: 4.07 ± 0.076
1.585AspSer: 1.585 ± 0.045
2.117AspThr: 2.117 ± 0.049
4.392AspVal: 4.392 ± 0.083
0.81AspTrp: 0.81 ± 0.042
1.126AspTyr: 1.126 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
10.424GluAla: 10.424 ± 0.151
0.478GluCys: 0.478 ± 0.028
2.605GluAsp: 2.605 ± 0.063
5.732GluGlu: 5.732 ± 0.118
2.185GluPhe: 2.185 ± 0.059
5.911GluGly: 5.911 ± 0.103
1.472GluHis: 1.472 ± 0.054
2.666GluIle: 2.666 ± 0.073
1.354GluLys: 1.354 ± 0.057
8.148GluLeu: 8.148 ± 0.108
1.218GluMet: 1.218 ± 0.044
1.184GluAsn: 1.184 ± 0.038
4.125GluPro: 4.125 ± 0.089
2.145GluGln: 2.145 ± 0.063
8.634GluArg: 8.634 ± 0.141
2.664GluSer: 2.664 ± 0.063
3.003GluThr: 3.003 ± 0.066
5.561GluVal: 5.561 ± 0.092
0.998GluTrp: 0.998 ± 0.038
1.514GluTyr: 1.514 ± 0.046
0.0GluXaa: 0.0 ± 0.0
Phe
4.825PheAla: 4.825 ± 0.101
0.35PheCys: 0.35 ± 0.021
2.192PheAsp: 2.192 ± 0.051
2.677PheGlu: 2.677 ± 0.062
1.233PhePhe: 1.233 ± 0.049
3.687PheGly: 3.687 ± 0.079
0.638PheHis: 0.638 ± 0.03
1.171PheIle: 1.171 ± 0.046
0.467PheLys: 0.467 ± 0.029
3.402PheLeu: 3.402 ± 0.094
0.431PheMet: 0.431 ± 0.025
0.635PheAsn: 0.635 ± 0.033
1.722PhePro: 1.722 ± 0.051
0.833PheGln: 0.833 ± 0.031
2.922PheArg: 2.922 ± 0.069
1.449PheSer: 1.449 ± 0.051
1.649PheThr: 1.649 ± 0.051
2.961PheVal: 2.961 ± 0.065
0.528PheTrp: 0.528 ± 0.03
0.752PheTyr: 0.752 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
10.007GlyAla: 10.007 ± 0.137
0.819GlyCys: 0.819 ± 0.04
4.168GlyAsp: 4.168 ± 0.083
5.851GlyGlu: 5.851 ± 0.101
3.275GlyPhe: 3.275 ± 0.075
7.718GlyGly: 7.718 ± 0.148
1.674GlyHis: 1.674 ± 0.056
4.394GlyIle: 4.394 ± 0.077
2.196GlyLys: 2.196 ± 0.054
8.614GlyLeu: 8.614 ± 0.135
1.984GlyMet: 1.984 ± 0.057
1.695GlyAsn: 1.695 ± 0.063
4.526GlyPro: 4.526 ± 0.082
2.441GlyGln: 2.441 ± 0.09
7.767GlyArg: 7.767 ± 0.106
4.361GlySer: 4.361 ± 0.099
4.868GlyThr: 4.868 ± 0.081
7.002GlyVal: 7.002 ± 0.102
1.583GlyTrp: 1.583 ± 0.06
2.419GlyTyr: 2.419 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
2.044HisAla: 2.044 ± 0.051
0.193HisCys: 0.193 ± 0.018
0.963HisAsp: 0.963 ± 0.035
1.196HisGlu: 1.196 ± 0.041
0.677HisPhe: 0.677 ± 0.03
1.795HisGly: 1.795 ± 0.052
0.523HisHis: 0.523 ± 0.027
0.82HisIle: 0.82 ± 0.04
0.225HisLys: 0.225 ± 0.018
2.059HisLeu: 2.059 ± 0.052
0.295HisMet: 0.295 ± 0.021
0.346HisAsn: 0.346 ± 0.021
1.544HisPro: 1.544 ± 0.044
0.505HisGln: 0.505 ± 0.027
1.718HisArg: 1.718 ± 0.054
0.644HisSer: 0.644 ± 0.028
0.888HisThr: 0.888 ± 0.034
1.513HisVal: 1.513 ± 0.049
0.345HisTrp: 0.345 ± 0.025
0.417HisTyr: 0.417 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.257IleAla: 6.257 ± 0.094
0.392IleCys: 0.392 ± 0.026
2.783IleAsp: 2.783 ± 0.065
3.487IleGlu: 3.487 ± 0.065
1.254IlePhe: 1.254 ± 0.051
3.708IleGly: 3.708 ± 0.08
0.815IleHis: 0.815 ± 0.033
1.717IleIle: 1.717 ± 0.062
0.641IleLys: 0.641 ± 0.033
3.678IleLeu: 3.678 ± 0.062
0.584IleMet: 0.584 ± 0.029
0.751IleAsn: 0.751 ± 0.032
2.373IlePro: 2.373 ± 0.057
1.001IleGln: 1.001 ± 0.036
3.61IleArg: 3.61 ± 0.071
1.653IleSer: 1.653 ± 0.052
1.87IleThr: 1.87 ± 0.063
4.1IleVal: 4.1 ± 0.069
0.464IleTrp: 0.464 ± 0.026
0.824IleTyr: 0.824 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
2.205LysAla: 2.205 ± 0.054
0.129LysCys: 0.129 ± 0.013
0.837LysAsp: 0.837 ± 0.042
1.442LysGlu: 1.442 ± 0.041
0.56LysPhe: 0.56 ± 0.028
1.553LysGly: 1.553 ± 0.048
0.367LysHis: 0.367 ± 0.026
0.759LysIle: 0.759 ± 0.032
0.569LysLys: 0.569 ± 0.035
2.081LysLeu: 2.081 ± 0.064
0.38LysMet: 0.38 ± 0.025
0.423LysAsn: 0.423 ± 0.027
1.237LysPro: 1.237 ± 0.052
0.564LysGln: 0.564 ± 0.03
1.778LysArg: 1.778 ± 0.046
0.792LysSer: 0.792 ± 0.031
0.915LysThr: 0.915 ± 0.039
1.732LysVal: 1.732 ± 0.054
0.196LysTrp: 0.196 ± 0.017
0.463LysTyr: 0.463 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
16.45LeuAla: 16.45 ± 0.24
0.863LeuCys: 0.863 ± 0.034
5.555LeuAsp: 5.555 ± 0.094
7.362LeuGlu: 7.362 ± 0.107
3.341LeuPhe: 3.341 ± 0.082
9.252LeuGly: 9.252 ± 0.135
1.827LeuHis: 1.827 ± 0.044
3.409LeuIle: 3.409 ± 0.066
1.845LeuLys: 1.845 ± 0.059
10.827LeuLeu: 10.827 ± 0.158
1.447LeuMet: 1.447 ± 0.043
1.62LeuAsn: 1.62 ± 0.049
6.52LeuPro: 6.52 ± 0.105
2.793LeuGln: 2.793 ± 0.063
10.225LeuArg: 10.225 ± 0.141
4.421LeuSer: 4.421 ± 0.088
4.717LeuThr: 4.717 ± 0.071
8.726LeuVal: 8.726 ± 0.125
1.378LeuTrp: 1.378 ± 0.049
1.987LeuTyr: 1.987 ± 0.05
0.0LeuXaa: 0.0 ± 0.0
Met
2.557MetAla: 2.557 ± 0.061
0.121MetCys: 0.121 ± 0.011
0.687MetAsp: 0.687 ± 0.037
1.126MetGlu: 1.126 ± 0.048
0.56MetPhe: 0.56 ± 0.032
1.457MetGly: 1.457 ± 0.046
0.328MetHis: 0.328 ± 0.022
0.553MetIle: 0.553 ± 0.028
0.473MetLys: 0.473 ± 0.029
1.841MetLeu: 1.841 ± 0.053
0.331MetMet: 0.331 ± 0.021
0.431MetAsn: 0.431 ± 0.023
1.221MetPro: 1.221 ± 0.043
0.498MetGln: 0.498 ± 0.028
1.713MetArg: 1.713 ± 0.055
1.055MetSer: 1.055 ± 0.036
1.262MetThr: 1.262 ± 0.045
1.44MetVal: 1.44 ± 0.048
0.188MetTrp: 0.188 ± 0.016
0.317MetTyr: 0.317 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
2.313AsnAla: 2.313 ± 0.06
0.192AsnCys: 0.192 ± 0.016
0.87AsnAsp: 0.87 ± 0.037
1.073AsnGlu: 1.073 ± 0.041
0.703AsnPhe: 0.703 ± 0.036
1.682AsnGly: 1.682 ± 0.054
0.332AsnHis: 0.332 ± 0.018
0.969AsnIle: 0.969 ± 0.038
0.299AsnLys: 0.299 ± 0.019
1.917AsnLeu: 1.917 ± 0.054
0.345AsnMet: 0.345 ± 0.022
0.392AsnAsn: 0.392 ± 0.026
1.661AsnPro: 1.661 ± 0.071
0.443AsnGln: 0.443 ± 0.026
1.504AsnArg: 1.504 ± 0.047
0.687AsnSer: 0.687 ± 0.033
0.834AsnThr: 0.834 ± 0.04
1.658AsnVal: 1.658 ± 0.052
0.264AsnTrp: 0.264 ± 0.023
0.452AsnTyr: 0.452 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
7.355ProAla: 7.355 ± 0.118
0.407ProCys: 0.407 ± 0.025
3.69ProAsp: 3.69 ± 0.073
5.372ProGlu: 5.372 ± 0.082
2.102ProPhe: 2.102 ± 0.054
5.946ProGly: 5.946 ± 0.111
1.143ProHis: 1.143 ± 0.044
2.121ProIle: 2.121 ± 0.06
1.293ProLys: 1.293 ± 0.049
5.743ProLeu: 5.743 ± 0.088
1.08ProMet: 1.08 ± 0.034
1.418ProAsn: 1.418 ± 0.044
4.59ProPro: 4.59 ± 0.115
1.432ProGln: 1.432 ± 0.036
4.685ProArg: 4.685 ± 0.074
3.316ProSer: 3.316 ± 0.084
3.277ProThr: 3.277 ± 0.105
4.793ProVal: 4.793 ± 0.079
1.019ProTrp: 1.019 ± 0.04
1.355ProTyr: 1.355 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
3.506GlnAla: 3.506 ± 0.064
0.179GlnCys: 0.179 ± 0.017
0.816GlnAsp: 0.816 ± 0.04
1.684GlnGlu: 1.684 ± 0.051
0.859GlnPhe: 0.859 ± 0.033
1.945GlnGly: 1.945 ± 0.071
0.56GlnHis: 0.56 ± 0.028
0.983GlnIle: 0.983 ± 0.04
0.57GlnLys: 0.57 ± 0.029
3.076GlnLeu: 3.076 ± 0.066
0.57GlnMet: 0.57 ± 0.03
0.516GlnAsn: 0.516 ± 0.03
1.856GlnPro: 1.856 ± 0.056
1.044GlnGln: 1.044 ± 0.045
2.979GlnArg: 2.979 ± 0.079
1.086GlnSer: 1.086 ± 0.043
1.105GlnThr: 1.105 ± 0.037
2.112GlnVal: 2.112 ± 0.053
0.363GlnTrp: 0.363 ± 0.02
0.641GlnTyr: 0.641 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
11.123ArgAla: 11.123 ± 0.149
0.901ArgCys: 0.901 ± 0.039
4.184ArgAsp: 4.184 ± 0.09
7.073ArgGlu: 7.073 ± 0.105
3.364ArgPhe: 3.364 ± 0.063
6.798ArgGly: 6.798 ± 0.105
1.768ArgHis: 1.768 ± 0.05
4.348ArgIle: 4.348 ± 0.08
1.702ArgLys: 1.702 ± 0.052
9.847ArgLeu: 9.847 ± 0.156
1.96ArgMet: 1.96 ± 0.048
1.685ArgAsn: 1.685 ± 0.047
5.295ArgPro: 5.295 ± 0.096
2.716ArgGln: 2.716 ± 0.068
9.532ArgArg: 9.532 ± 0.14
4.055ArgSer: 4.055 ± 0.071
4.35ArgThr: 4.35 ± 0.072
6.713ArgVal: 6.713 ± 0.098
1.786ArgTrp: 1.786 ± 0.054
2.392ArgTyr: 2.392 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
5.301SerAla: 5.301 ± 0.105
0.339SerCys: 0.339 ± 0.022
2.045SerAsp: 2.045 ± 0.051
2.785SerGlu: 2.785 ± 0.056
1.749SerPhe: 1.749 ± 0.047
4.41SerGly: 4.41 ± 0.104
0.719SerHis: 0.719 ± 0.033
1.934SerIle: 1.934 ± 0.046
0.767SerLys: 0.767 ± 0.034
4.684SerLeu: 4.684 ± 0.092
0.988SerMet: 0.988 ± 0.041
0.781SerAsn: 0.781 ± 0.04
3.224SerPro: 3.224 ± 0.078
1.148SerGln: 1.148 ± 0.043
3.416SerArg: 3.416 ± 0.069
2.184SerSer: 2.184 ± 0.081
2.073SerThr: 2.073 ± 0.059
3.573SerVal: 3.573 ± 0.077
0.737SerTrp: 0.737 ± 0.035
0.986SerTyr: 0.986 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
5.954ThrAla: 5.954 ± 0.092
0.36ThrCys: 0.36 ± 0.022
2.13ThrAsp: 2.13 ± 0.055
2.672ThrGlu: 2.672 ± 0.06
1.824ThrPhe: 1.824 ± 0.058
4.571ThrGly: 4.571 ± 0.084
0.845ThrHis: 0.845 ± 0.031
2.379ThrIle: 2.379 ± 0.066
0.981ThrLys: 0.981 ± 0.035
4.948ThrLeu: 4.948 ± 0.089
0.813ThrMet: 0.813 ± 0.037
1.019ThrAsn: 1.019 ± 0.039
3.863ThrPro: 3.863 ± 0.125
1.047ThrGln: 1.047 ± 0.042
3.115ThrArg: 3.115 ± 0.062
2.237ThrSer: 2.237 ± 0.062
2.194ThrThr: 2.194 ± 0.056
4.618ThrVal: 4.618 ± 0.105
0.698ThrTrp: 0.698 ± 0.033
1.14ThrTyr: 1.14 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
11.095ValAla: 11.095 ± 0.142
0.73ValCys: 0.73 ± 0.032
4.089ValAsp: 4.089 ± 0.076
5.814ValGlu: 5.814 ± 0.094
2.74ValPhe: 2.74 ± 0.063
6.62ValGly: 6.62 ± 0.087
1.54ValHis: 1.54 ± 0.046
3.817ValIle: 3.817 ± 0.073
1.501ValLys: 1.501 ± 0.053
8.729ValLeu: 8.729 ± 0.122
1.421ValMet: 1.421 ± 0.046
1.663ValAsn: 1.663 ± 0.057
5.259ValPro: 5.259 ± 0.093
1.992ValGln: 1.992 ± 0.053
7.846ValArg: 7.846 ± 0.108
3.645ValSer: 3.645 ± 0.074
4.507ValThr: 4.507 ± 0.09
8.034ValVal: 8.034 ± 0.123
1.154ValTrp: 1.154 ± 0.041
1.77ValTyr: 1.77 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
1.707TrpAla: 1.707 ± 0.058
0.188TrpCys: 0.188 ± 0.015
0.762TrpAsp: 0.762 ± 0.033
1.087TrpGlu: 1.087 ± 0.038
0.612TrpPhe: 0.612 ± 0.029
1.171TrpGly: 1.171 ± 0.049
0.343TrpHis: 0.343 ± 0.023
0.439TrpIle: 0.439 ± 0.025
0.335TrpLys: 0.335 ± 0.023
1.795TrpLeu: 1.795 ± 0.057
0.331TrpMet: 0.331 ± 0.022
0.364TrpAsn: 0.364 ± 0.02
0.827TrpPro: 0.827 ± 0.044
0.569TrpGln: 0.569 ± 0.027
1.533TrpArg: 1.533 ± 0.045
0.723TrpSer: 0.723 ± 0.034
0.716TrpThr: 0.716 ± 0.032
1.161TrpVal: 1.161 ± 0.038
0.356TrpTrp: 0.356 ± 0.025
0.42TrpTyr: 0.42 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.516TyrAla: 2.516 ± 0.071
0.302TyrCys: 0.302 ± 0.019
1.28TyrAsp: 1.28 ± 0.051
1.546TyrGlu: 1.546 ± 0.051
0.902TyrPhe: 0.902 ± 0.036
2.056TyrGly: 2.056 ± 0.053
0.503TyrHis: 0.503 ± 0.028
0.784TyrIle: 0.784 ± 0.036
0.373TyrLys: 0.373 ± 0.025
2.515TyrLeu: 2.515 ± 0.061
0.296TyrMet: 0.296 ± 0.02
0.407TyrAsn: 0.407 ± 0.029
1.289TyrPro: 1.289 ± 0.044
0.644TyrGln: 0.644 ± 0.032
2.313TyrArg: 2.313 ± 0.052
0.93TyrSer: 0.93 ± 0.035
1.033TyrThr: 1.033 ± 0.038
1.845TyrVal: 1.845 ± 0.058
0.361TyrTrp: 0.361 ± 0.02
0.577TyrTyr: 0.577 ± 0.031
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2317 proteins (719333 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski