Streptococcus satellite phage Javan279
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZI14|A0A4D5ZI14_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan279 OX=2558602 GN=JavanS279_0006 PE=4 SV=1
MM1 pKa = 7.82 ADD3 pKa = 4.14 KK4 pKa = 11.05 KK5 pKa = 11.09 DD6 pKa = 3.37 FDD8 pKa = 4.66 LANEE12 pKa = 3.94 RR13 pKa = 11.84 AKK15 pKa = 11.2 NFGIWLEE22 pKa = 4.04 EE23 pKa = 4.33 AYY25 pKa = 8.85 QTMLDD30 pKa = 4.45 FSLEE34 pKa = 4.24 DD35 pKa = 4.04 KK36 pKa = 10.56 FDD38 pKa = 4.03 CYY40 pKa = 11.03 SIEE43 pKa = 4.08 EE44 pKa = 4.21 RR45 pKa = 11.84 NQLEE49 pKa = 4.34 RR50 pKa = 11.84 VLEE53 pKa = 4.07 TLMDD57 pKa = 4.18 FCDD60 pKa = 3.07 MWEE63 pKa = 4.02 RR64 pKa = 11.84 GQIILASKK72 pKa = 9.62 EE73 pKa = 3.85 RR74 pKa = 11.84 EE75 pKa = 4.13 AIEE78 pKa = 3.87
Molecular weight: 9.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.45
IPC2_protein 4.304
IPC_protein 4.202
Toseland 4.037
ProMoST 4.266
Dawson 4.151
Bjellqvist 4.355
Wikipedia 4.024
Rodwell 4.037
Grimsley 3.948
Solomon 4.139
Lehninger 4.101
Nozaki 4.266
DTASelect 4.406
Thurlkill 4.05
EMBOSS 4.05
Sillero 4.304
Patrickios 3.897
IPC_peptide 4.151
IPC2_peptide 4.291
IPC2.peptide.svr19 4.236
Protein with the highest isoelectric point:
>tr|A0A4D5ZHP1|A0A4D5ZHP1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan279 OX=2558602 GN=JavanS279_0008 PE=4 SV=1
MM1 pKa = 7.78 KK2 pKa = 10.59 LPTLPEE8 pKa = 3.71 NYY10 pKa = 9.48 KK11 pKa = 10.42 RR12 pKa = 11.84 VLALIPQGAEE22 pKa = 3.44 RR23 pKa = 11.84 PRR25 pKa = 11.84 AGKK28 pKa = 10.2 EE29 pKa = 3.73 IVKK32 pKa = 8.96 LTGLDD37 pKa = 2.96 IRR39 pKa = 11.84 TVRR42 pKa = 11.84 GIIRR46 pKa = 11.84 GLVIKK51 pKa = 8.25 YY52 pKa = 9.67 HH53 pKa = 5.96 IAICGSRR60 pKa = 11.84 DD61 pKa = 3.31 LAGGYY66 pKa = 8.04 YY67 pKa = 9.75 IPANDD72 pKa = 3.72 SEE74 pKa = 4.4 RR75 pKa = 11.84 LAGIRR80 pKa = 11.84 ALDD83 pKa = 3.66 RR84 pKa = 11.84 QRR86 pKa = 11.84 QDD88 pKa = 2.9 EE89 pKa = 4.25 QKK91 pKa = 10.55 RR92 pKa = 11.84 ISALMYY98 pKa = 10.81 ADD100 pKa = 5.64 LNEE103 pKa = 3.71 QDD105 pKa = 4.95 KK106 pKa = 10.69 YY107 pKa = 11.77 LKK109 pKa = 10.82 GSS111 pKa = 3.38
Molecular weight: 12.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.169
IPC2_protein 9.224
IPC_protein 9.311
Toseland 9.78
ProMoST 9.56
Dawson 10.043
Bjellqvist 9.75
Wikipedia 10.248
Rodwell 10.306
Grimsley 10.131
Solomon 10.087
Lehninger 10.058
Nozaki 9.794
DTASelect 9.75
Thurlkill 9.882
EMBOSS 10.204
Sillero 9.955
Patrickios 7.614
IPC_peptide 10.087
IPC2_peptide 8.434
IPC2.peptide.svr19 8.223
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
0
18
2735
39
381
151.9
17.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.837 ± 0.539
0.585 ± 0.147
5.155 ± 0.527
7.861 ± 0.624
4.168 ± 0.407
4.534 ± 0.468
1.463 ± 0.29
7.313 ± 0.442
9.543 ± 0.368
11.517 ± 0.775
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.413 ± 0.368
5.631 ± 0.704
2.523 ± 0.261
4.973 ± 0.428
4.68 ± 0.523
5.046 ± 0.278
5.887 ± 0.675
4.424 ± 0.442
1.133 ± 0.219
4.314 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here