Roseobacter cerasinus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseobacter

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4343 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A640VQK1|A0A640VQK1_9RHOB Shikimate kinase OS=Roseobacter cerasinus OX=2602289 GN=aroK_1 PE=3 SV=1
MM1 pKa = 7.48SNIDD5 pKa = 3.97PSQSSQNPEE14 pKa = 3.88TNQDD18 pKa = 3.82DD19 pKa = 4.46LSLHH23 pKa = 7.0LSTCTCAACMADD35 pKa = 6.09DD36 pKa = 4.95RR37 pKa = 11.84FQDD40 pKa = 3.58DD41 pKa = 3.98VEE43 pKa = 4.69RR44 pKa = 11.84SGNPTSGGGTAAAATGKK61 pKa = 7.26TLRR64 pKa = 11.84EE65 pKa = 3.75MADD68 pKa = 3.87FLEE71 pKa = 4.22MGYY74 pKa = 9.48WGNEE78 pKa = 3.16VGLRR82 pKa = 11.84HH83 pKa = 6.02NVGTTGLDD91 pKa = 3.41PNNGVLHH98 pKa = 5.88YY99 pKa = 9.47NVSGFGPLTYY109 pKa = 10.63GGGSDD114 pKa = 3.86ANGVSSARR122 pKa = 11.84AALIRR127 pKa = 11.84DD128 pKa = 3.45AFDD131 pKa = 2.94VYY133 pKa = 10.9EE134 pKa = 4.47AVLGIQFVEE143 pKa = 4.6TTSTNDD149 pKa = 3.14SVVDD153 pKa = 4.84FFFSDD158 pKa = 3.5NQSGAFAGSTRR169 pKa = 11.84YY170 pKa = 10.38GDD172 pKa = 3.26GTIYY176 pKa = 10.68YY177 pKa = 9.89SYY179 pKa = 11.3INIAASWSGSSSTYY193 pKa = 11.06DD194 pKa = 3.7DD195 pKa = 3.89YY196 pKa = 11.86TLQTIFHH203 pKa = 7.27EE204 pKa = 4.72IGHH207 pKa = 6.51ALGLGHH213 pKa = 6.5QGPYY217 pKa = 10.01NGSASYY223 pKa = 11.6ANDD226 pKa = 4.42AIFALDD232 pKa = 3.13SWQASMMSYY241 pKa = 10.57FSQTEE246 pKa = 3.62NSAISASYY254 pKa = 10.81EE255 pKa = 3.91FLQTPMAVDD264 pKa = 3.78WLALDD269 pKa = 4.84SIYY272 pKa = 10.8GQQGYY277 pKa = 9.76GISNAFVGDD286 pKa = 3.46TVYY289 pKa = 11.14GFNTNIAASEE299 pKa = 3.96SRR301 pKa = 11.84IWNEE305 pKa = 3.12FSAYY309 pKa = 10.06AGRR312 pKa = 11.84TASTIVDD319 pKa = 3.39GGGIDD324 pKa = 3.45TLDD327 pKa = 3.51FSGYY331 pKa = 9.78SANQKK336 pKa = 9.74IDD338 pKa = 3.67LTVQTADD345 pKa = 3.13QTSQNTSNVGGRR357 pKa = 11.84TGNLTLAIGTVIEE370 pKa = 4.1NAIGGSGNDD379 pKa = 3.24YY380 pKa = 10.94FIGNEE385 pKa = 3.63ADD387 pKa = 3.25NVFRR391 pKa = 11.84GGAGNDD397 pKa = 3.16RR398 pKa = 11.84FIGQLGDD405 pKa = 3.63DD406 pKa = 4.17TFHH409 pKa = 7.0GNAGLDD415 pKa = 3.39TVIYY419 pKa = 9.92NLVFGAYY426 pKa = 9.16SFSVLSDD433 pKa = 3.53VIQVIGEE440 pKa = 4.56GIDD443 pKa = 3.57LVYY446 pKa = 10.06DD447 pKa = 4.17TIEE450 pKa = 3.98TFQFSDD456 pKa = 2.78ITYY459 pKa = 9.64SFTDD463 pKa = 3.28IFALVGNAVPTARR476 pKa = 11.84DD477 pKa = 3.23DD478 pKa = 3.74SATVEE483 pKa = 4.12EE484 pKa = 5.26GATITLSVLDD494 pKa = 4.91NDD496 pKa = 4.55TDD498 pKa = 4.31PDD500 pKa = 4.39GDD502 pKa = 4.1PLTITEE508 pKa = 4.54VNGQAIGVNEE518 pKa = 4.25TVDD521 pKa = 3.96LASGASVTLRR531 pKa = 11.84DD532 pKa = 5.0DD533 pKa = 3.57GTLSYY538 pKa = 10.5AQNGVFDD545 pKa = 4.09TLNTGEE551 pKa = 4.34SGGDD555 pKa = 3.21SFEE558 pKa = 4.41YY559 pKa = 10.53AVSDD563 pKa = 3.99GKK565 pKa = 11.26GGAATATVVMTVEE578 pKa = 4.45GAGDD582 pKa = 4.21DD583 pKa = 4.11SPSAAIGQSGTVTVAQSGSDD603 pKa = 2.97QWHH606 pKa = 5.27RR607 pKa = 11.84VVFDD611 pKa = 3.32TTISDD616 pKa = 3.66AVVVMGPISWNGLQAATTRR635 pKa = 11.84VRR637 pKa = 11.84NVTDD641 pKa = 3.26EE642 pKa = 4.15GFEE645 pKa = 3.95FQIDD649 pKa = 3.22EE650 pKa = 4.19WNYY653 pKa = 10.8LDD655 pKa = 4.96GAHH658 pKa = 5.17TTEE661 pKa = 4.54TIGWLAMSEE670 pKa = 4.41GSHH673 pKa = 5.5TLSNGQTIVAGTQIVGTEE691 pKa = 4.19FSSVSFGEE699 pKa = 4.08VLGDD703 pKa = 3.38AVVLAEE709 pKa = 4.21VSTANGKK716 pKa = 9.51DD717 pKa = 3.19AVMTRR722 pKa = 11.84LNDD725 pKa = 3.27VTASGFEE732 pKa = 4.01VQLQEE737 pKa = 4.2EE738 pKa = 4.69EE739 pKa = 4.3ALGAHH744 pKa = 5.92TDD746 pKa = 3.7EE747 pKa = 4.48TVSWIALEE755 pKa = 4.14TGVGDD760 pKa = 3.69GLDD763 pKa = 3.44VFRR766 pKa = 11.84TADD769 pKa = 3.58QLDD772 pKa = 3.98HH773 pKa = 6.83EE774 pKa = 4.8VDD776 pKa = 3.27SFQFNTSFAKK786 pKa = 10.73APVILGDD793 pKa = 3.94LQSKK797 pKa = 10.21DD798 pKa = 3.38GRR800 pKa = 11.84DD801 pKa = 3.44PATLRR806 pKa = 11.84LSQVDD811 pKa = 3.5RR812 pKa = 11.84SGVSLFVEE820 pKa = 4.93EE821 pKa = 4.21EE822 pKa = 4.03TSADD826 pKa = 3.4SEE828 pKa = 4.72VIHH831 pKa = 6.12TEE833 pKa = 3.58EE834 pKa = 3.79TAGYY838 pKa = 8.09VALVEE843 pKa = 4.13GLIFEE848 pKa = 5.4DD849 pKa = 4.13SFLFF853 pKa = 4.46

Molecular weight: 90.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A640VM94|A0A640VM94_9RHOB Uncharacterized protein OS=Roseobacter cerasinus OX=2602289 GN=So717_02830 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.34SLSAA44 pKa = 3.83

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4343

0

4343

1330952

39

3496

306.5

33.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.165 ± 0.051

0.927 ± 0.012

6.201 ± 0.044

5.778 ± 0.038

3.813 ± 0.022

8.518 ± 0.043

2.083 ± 0.021

5.189 ± 0.026

3.056 ± 0.031

10.032 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.79 ± 0.02

2.579 ± 0.024

5.015 ± 0.029

3.499 ± 0.02

6.478 ± 0.038

5.268 ± 0.025

5.682 ± 0.034

7.319 ± 0.028

1.38 ± 0.016

2.229 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski