Salmonella phage SE1 (in:P22virus)
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B8K1E1|B8K1E1_9CAUD Kil OS=Salmonella phage SE1 (in:P22virus) OX=329864 GN=orf17 PE=4 SV=1
MM1 pKa = 7.29 LHH3 pKa = 7.3 DD4 pKa = 4.58 FDD6 pKa = 7.13 DD7 pKa = 5.13 DD8 pKa = 3.82 EE9 pKa = 5.6 FIALISPEE17 pKa = 3.74 IEE19 pKa = 3.94 EE20 pKa = 4.61 EE21 pKa = 4.24 VEE23 pKa = 4.03 QQINLAAEE31 pKa = 4.08 RR32 pKa = 11.84 QNPIIGWDD40 pKa = 3.51 EE41 pKa = 3.66 FAGYY45 pKa = 10.64 YY46 pKa = 9.62 SS47 pKa = 3.45
Molecular weight: 5.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.905
IPC2_protein 3.605
IPC_protein 3.465
Toseland 3.3
ProMoST 3.656
Dawson 3.465
Bjellqvist 3.63
Wikipedia 3.401
Rodwell 3.325
Grimsley 3.236
Solomon 3.401
Lehninger 3.35
Nozaki 3.617
DTASelect 3.706
Thurlkill 3.389
EMBOSS 3.414
Sillero 3.592
Patrickios 1.761
IPC_peptide 3.401
IPC2_peptide 3.554
IPC2.peptide.svr19 3.65
Protein with the highest isoelectric point:
>tr|B8K1G1|B8K1G1_9CAUD NinX OS=Salmonella phage SE1 (in:P22virus) OX=329864 GN=orf37 PE=4 SV=1
MM1 pKa = 7.39 ARR3 pKa = 11.84 QRR5 pKa = 11.84 RR6 pKa = 11.84 SITDD10 pKa = 4.1 IICEE14 pKa = 3.96 NCKK17 pKa = 10.21 YY18 pKa = 10.77 LPTKK22 pKa = 10.48 RR23 pKa = 11.84 SRR25 pKa = 11.84 NKK27 pKa = 9.55 PKK29 pKa = 10.29 PIPKK33 pKa = 9.96 EE34 pKa = 3.93 SDD36 pKa = 3.19 VKK38 pKa = 9.6 TFNYY42 pKa = 7.51 TAHH45 pKa = 6.73 LWDD48 pKa = 3.61 IRR50 pKa = 11.84 WLRR53 pKa = 11.84 EE54 pKa = 3.64 RR55 pKa = 11.84 ARR57 pKa = 11.84 KK58 pKa = 8.87
Molecular weight: 7.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.663
IPC_protein 10.189
Toseland 10.745
ProMoST 10.423
Dawson 10.833
Bjellqvist 10.482
Wikipedia 10.994
Rodwell 11.169
Grimsley 10.862
Solomon 10.921
Lehninger 10.906
Nozaki 10.73
DTASelect 10.482
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.76
Patrickios 10.935
IPC_peptide 10.935
IPC2_peptide 9.443
IPC2.peptide.svr19 8.616
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
67
0
67
12626
25
725
188.4
21.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.203 ± 0.522
1.339 ± 0.165
5.948 ± 0.273
6.336 ± 0.359
3.667 ± 0.289
6.906 ± 0.39
1.766 ± 0.178
6.178 ± 0.369
6.17 ± 0.304
7.896 ± 0.28
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.867 ± 0.167
4.808 ± 0.237
3.96 ± 0.175
4.507 ± 0.354
5.901 ± 0.264
6.423 ± 0.309
5.402 ± 0.234
5.893 ± 0.252
1.6 ± 0.162
3.231 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here