Synechococcus phage S-SM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Thetisvirus; Synechococcus virus SSM1

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3SIN4|E3SIN4_9CAUD Uncharacterized protein OS=Synechococcus phage S-SM1 OX=444859 GN=SSM1_228 PE=4 SV=1
MM1 pKa = 7.74AYY3 pKa = 9.79YY4 pKa = 10.27YY5 pKa = 9.95PEE7 pKa = 4.45GAFGPICDD15 pKa = 5.26LPLTDD20 pKa = 4.82AQIIEE25 pKa = 4.3QGRR28 pKa = 11.84SAISIDD34 pKa = 3.29EE35 pKa = 4.14GLIEE39 pKa = 4.2QYY41 pKa = 11.21GVVPGIYY48 pKa = 10.17DD49 pKa = 3.46EE50 pKa = 6.08FLDD53 pKa = 4.42FWGEE57 pKa = 3.78FNFPVFLRR65 pKa = 11.84RR66 pKa = 11.84KK67 pKa = 8.33CKK69 pKa = 10.21EE70 pKa = 3.65RR71 pKa = 11.84VLDD74 pKa = 4.65DD75 pKa = 3.47GTIEE79 pKa = 5.33KK80 pKa = 9.97FDD82 pKa = 4.15CVDD85 pKa = 3.32EE86 pKa = 4.3YY87 pKa = 11.73SRR89 pKa = 11.84PLPTSQEE96 pKa = 4.33SFVSATDD103 pKa = 4.47DD104 pKa = 3.9IGLKK108 pKa = 10.7DD109 pKa = 4.57LFFEE113 pKa = 4.8PKK115 pKa = 8.91LTPEE119 pKa = 3.99TCSPYY124 pKa = 10.28QADD127 pKa = 3.42INIRR131 pKa = 11.84PLKK134 pKa = 10.33FFGPNGVLIKK144 pKa = 9.47RR145 pKa = 11.84TPVEE149 pKa = 3.83GSTPVTFPVTSEE161 pKa = 4.0NQSIPNNATITATFSEE177 pKa = 4.54DD178 pKa = 3.15RR179 pKa = 11.84QNLVIGGTGEE189 pKa = 4.76GIVQLRR195 pKa = 11.84FTWDD199 pKa = 3.58DD200 pKa = 3.53KK201 pKa = 11.52PSISGLAVGTLTVAGASFTQSGEE224 pKa = 4.01KK225 pKa = 10.81GSDD228 pKa = 3.28NDD230 pKa = 3.61STSIVTAGQSYY241 pKa = 9.44PITLSGNSGTSGSRR255 pKa = 11.84LQGNTQIEE263 pKa = 4.39YY264 pKa = 10.8DD265 pKa = 3.87DD266 pKa = 5.03DD267 pKa = 4.52VPGFDD272 pKa = 4.21VNATLSITEE281 pKa = 4.12VLPTTTSTNVAGYY294 pKa = 9.57WSDD297 pKa = 3.28EE298 pKa = 3.96GNKK301 pKa = 10.01YY302 pKa = 9.91GVWVNPAICTLPQQPQSVSYY322 pKa = 10.54KK323 pKa = 9.09ITIDD327 pKa = 3.42EE328 pKa = 4.48ADD330 pKa = 3.35TYY332 pKa = 11.46GFTFGCDD339 pKa = 3.2DD340 pKa = 4.35NATLTINDD348 pKa = 3.73EE349 pKa = 4.15TSAFLTAVGGIFEE362 pKa = 4.7GGSYY366 pKa = 7.55NTPYY370 pKa = 10.26TGTRR374 pKa = 11.84ALTAGTLILTVNCTNSDD391 pKa = 4.06AGFQDD396 pKa = 4.48ANGDD400 pKa = 4.03PTGLAYY406 pKa = 10.23DD407 pKa = 3.89WQRR410 pKa = 11.84NPGGWYY416 pKa = 9.74IKK418 pKa = 8.67MCKK421 pKa = 10.03GGLCASGTNINWVPVGPHH439 pKa = 6.26PAWSDD444 pKa = 3.58FMDD447 pKa = 4.81EE448 pKa = 4.32YY449 pKa = 11.67AVFPSNIDD457 pKa = 3.41PLLDD461 pKa = 3.32QTQQATWNINIPTTGNYY478 pKa = 8.38TFEE481 pKa = 4.57CSADD485 pKa = 3.48NNATFTLDD493 pKa = 3.08GTQIATSNSFTTTTSVSLTNISEE516 pKa = 4.57GPHH519 pKa = 5.19TLVVSVFNGTNSNGANEE536 pKa = 4.21WTTNPGGVAWRR547 pKa = 11.84IQYY550 pKa = 10.69GGGSIDD556 pKa = 5.95ANFQSNGRR564 pKa = 11.84LLVTGAGAARR574 pKa = 11.84LDD576 pKa = 5.42FDD578 pKa = 5.96FEE580 pKa = 4.43WDD582 pKa = 4.53DD583 pKa = 4.11NPSTYY588 pKa = 9.85DD589 pKa = 3.25TALGTVAYY597 pKa = 7.31PQLGVSFTQNTGSSSGSDD615 pKa = 2.92SDD617 pKa = 3.82NVNNVTAGDD626 pKa = 3.83YY627 pKa = 11.17GVTILNNSGGFTVEE641 pKa = 3.78NSGKK645 pKa = 9.95RR646 pKa = 11.84LCFKK650 pKa = 10.92DD651 pKa = 5.35LDD653 pKa = 4.46GNDD656 pKa = 4.12CNAQLDD662 pKa = 4.0LSIVQGDD669 pKa = 3.66ATIASSLDD677 pKa = 3.54LNTPGDD683 pKa = 4.26GNLFWHH689 pKa = 6.4TRR691 pKa = 11.84LATGYY696 pKa = 8.63TFLEE700 pKa = 4.92DD701 pKa = 3.27NN702 pKa = 4.34

Molecular weight: 75.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3SIE5|E3SIE5_9CAUD Uncharacterized protein OS=Synechococcus phage S-SM1 OX=444859 GN=SSM1_138 PE=4 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84GGKK5 pKa = 8.73MIPNSTYY12 pKa = 10.2IIVPSYY18 pKa = 10.56GVRR21 pKa = 11.84SCTIFPRR28 pKa = 11.84AINN31 pKa = 3.46

Molecular weight: 3.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

234

0

234

56423

31

6180

241.1

26.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.203 ± 0.278

0.884 ± 0.115

6.692 ± 0.141

6.107 ± 0.317

4.333 ± 0.101

7.823 ± 0.399

1.581 ± 0.165

6.072 ± 0.185

5.718 ± 0.394

7.378 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.263

5.601 ± 0.186

3.97 ± 0.159

3.711 ± 0.097

4.13 ± 0.236

6.919 ± 0.225

7.669 ± 0.558

6.698 ± 0.236

1.102 ± 0.092

4.218 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski