Clostridium pasteurianum BC1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium pasteurianum

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4K2P4|R4K2P4_CLOPA Uncharacterized protein OS=Clostridium pasteurianum BC1 OX=86416 GN=Clopa_2516 PE=4 SV=1
MM1 pKa = 8.16DD2 pKa = 4.19KK3 pKa = 11.15QNIIKK8 pKa = 9.98FLEE11 pKa = 4.15EE12 pKa = 3.73NQIEE16 pKa = 4.52DD17 pKa = 3.46IEE19 pKa = 4.42EE20 pKa = 3.5IKK22 pKa = 10.91YY23 pKa = 10.48RR24 pKa = 11.84EE25 pKa = 3.82EE26 pKa = 4.25VYY28 pKa = 10.03TLRR31 pKa = 11.84FYY33 pKa = 11.74YY34 pKa = 10.64DD35 pKa = 3.47FDD37 pKa = 4.83DD38 pKa = 5.4SEE40 pKa = 4.84IEE42 pKa = 3.94AARR45 pKa = 11.84AYY47 pKa = 10.72ANDD50 pKa = 3.4EE51 pKa = 4.26CEE53 pKa = 4.14EE54 pKa = 4.13EE55 pKa = 4.4EE56 pKa = 4.95EE57 pKa = 4.89GEE59 pKa = 4.08VWYY62 pKa = 10.99GEE64 pKa = 4.04FFKK67 pKa = 10.67PYY69 pKa = 10.34LNDD72 pKa = 3.21IVIDD76 pKa = 4.18NIGDD80 pKa = 3.67ILEE83 pKa = 4.63DD84 pKa = 3.54CMKK87 pKa = 9.91EE88 pKa = 3.98FNIGIQFISYY98 pKa = 10.66DD99 pKa = 3.57FDD101 pKa = 5.78DD102 pKa = 5.15EE103 pKa = 4.53EE104 pKa = 4.32QCSEE108 pKa = 4.29IIAIFYY114 pKa = 10.63EE115 pKa = 3.98SDD117 pKa = 3.12KK118 pKa = 11.43VVDD121 pKa = 3.75IEE123 pKa = 5.38NVIDD127 pKa = 4.5DD128 pKa = 4.65LKK130 pKa = 11.38LL131 pKa = 3.84

Molecular weight: 15.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4JY66|R4JY66_CLOPA Putative transcriptional regulator with cupin domain OS=Clostridium pasteurianum BC1 OX=86416 GN=Clopa_0718 PE=4 SV=1
MM1 pKa = 7.36SRR3 pKa = 11.84EE4 pKa = 4.11NGNSRR9 pKa = 11.84DD10 pKa = 3.68GRR12 pKa = 11.84DD13 pKa = 3.5GRR15 pKa = 11.84DD16 pKa = 3.24SRR18 pKa = 11.84KK19 pKa = 9.93GGPRR23 pKa = 11.84GRR25 pKa = 11.84RR26 pKa = 11.84MKK28 pKa = 10.65RR29 pKa = 11.84KK30 pKa = 9.0VCSFCMDD37 pKa = 3.42KK38 pKa = 10.98ADD40 pKa = 4.68HH41 pKa = 7.03IDD43 pKa = 3.49YY44 pKa = 11.12KK45 pKa = 11.11DD46 pKa = 3.21INKK49 pKa = 9.01LRR51 pKa = 11.84KK52 pKa = 9.78YY53 pKa = 8.01ITEE56 pKa = 4.01RR57 pKa = 11.84GKK59 pKa = 10.04ILPRR63 pKa = 11.84RR64 pKa = 11.84ISGNCAKK71 pKa = 9.64HH72 pKa = 5.56QRR74 pKa = 11.84MLTEE78 pKa = 4.16SIKK81 pKa = 10.25RR82 pKa = 11.84SRR84 pKa = 11.84NIALLPFTTEE94 pKa = 3.59

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4454

0

4454

1282591

29

3140

288.0

32.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.813 ± 0.04

1.182 ± 0.016

5.476 ± 0.032

6.813 ± 0.047

4.461 ± 0.028

6.362 ± 0.04

1.432 ± 0.015

10.067 ± 0.047

8.954 ± 0.046

8.93 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.515 ± 0.017

6.532 ± 0.04

2.929 ± 0.024

2.536 ± 0.022

3.385 ± 0.026

6.461 ± 0.03

4.99 ± 0.031

6.305 ± 0.03

0.768 ± 0.012

4.087 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski