Streptomyces albus (strain ATCC 21838 / DSM 41398 / FERM P-419 / JCM 4703 / NBRC 107858)
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7322 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5ETY6|A0A0B5ETY6_STRA4 Uncharacterized protein OS=Streptomyces albus (strain ATCC 21838 / DSM 41398 / FERM P-419 / JCM 4703 / NBRC 107858) OX=1081613 GN=SLNWT_5917 PE=4 SV=1
MM1 pKa = 7.48 HH2 pKa = 7.33 KK3 pKa = 10.05 LARR6 pKa = 11.84 RR7 pKa = 11.84 FAVVAAAGGFALGLTATSASAGAQATWTVAPGGAITAHH45 pKa = 6.81 ADD47 pKa = 3.42 VPTLTVPAATLTCDD61 pKa = 3.45 GSDD64 pKa = 2.92 ATGSVKK70 pKa = 10.57 SGSGLNGAGIGQIDD84 pKa = 4.38 SLTFDD89 pKa = 3.33 NCGLAGISFEE99 pKa = 4.36 VTASGTPWALNVSGVNSGDD118 pKa = 3.71 PDD120 pKa = 3.66 RR121 pKa = 11.84 VDD123 pKa = 3.37 GTITGITAHH132 pKa = 6.91 IEE134 pKa = 4.17 GTGCSADD141 pKa = 3.44 FSGDD145 pKa = 3.19 VTGYY149 pKa = 10.48 FDD151 pKa = 3.68 NATSEE156 pKa = 4.34 LVVDD160 pKa = 4.5 GGGDD164 pKa = 3.57 LTASNANCLGLINDD178 pKa = 3.81 GDD180 pKa = 4.06 VASFEE185 pKa = 3.96 AAYY188 pKa = 10.25 AVTPGQTITLDD199 pKa = 3.19
Molecular weight: 19.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.795
IPC_protein 3.795
Toseland 3.567
ProMoST 3.935
Dawson 3.808
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.617
Grimsley 3.478
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.228
Thurlkill 3.643
EMBOSS 3.795
Sillero 3.923
Patrickios 1.914
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A0B5EK14|A0A0B5EK14_STRA4 Putative transcriptional regulator OS=Streptomyces albus (strain ATCC 21838 / DSM 41398 / FERM P-419 / JCM 4703 / NBRC 107858) OX=1081613 GN=SLNWT_2267 PE=4 SV=1
MM1 pKa = 6.97 VRR3 pKa = 11.84 SLRR6 pKa = 11.84 VATRR10 pKa = 11.84 RR11 pKa = 11.84 ARR13 pKa = 11.84 MARR16 pKa = 11.84 TPRR19 pKa = 11.84 TGPGTRR25 pKa = 11.84 GSRR28 pKa = 11.84 RR29 pKa = 11.84 GAPRR33 pKa = 11.84 PAPVV37 pKa = 3.03
Molecular weight: 4.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.501
Grimsley 13.042
Solomon 13.495
Lehninger 13.393
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.237
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7322
0
7322
2436543
37
8023
332.8
35.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.005 ± 0.048
0.793 ± 0.007
5.579 ± 0.022
6.273 ± 0.028
2.646 ± 0.017
9.899 ± 0.032
2.353 ± 0.015
2.736 ± 0.021
2.053 ± 0.025
10.652 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.581 ± 0.012
1.547 ± 0.014
6.492 ± 0.031
2.805 ± 0.016
8.403 ± 0.034
5.163 ± 0.023
5.832 ± 0.025
7.807 ± 0.03
1.44 ± 0.012
1.941 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here