Hibiscus chlorotic ringspot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Betacarmovirus

Average proteome isoelectric point is 8.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9IR82|Q9IR82_9TOMB Uncharacterized protein OS=Hibiscus chlorotic ringspot virus OX=53181 PE=4 SV=1
MM1 pKa = 7.98ASPQSNDD8 pKa = 2.7SFIEE12 pKa = 4.06LNPHH16 pKa = 5.02NTGDD20 pKa = 3.65SKK22 pKa = 11.64GAGKK26 pKa = 10.66GNRR29 pKa = 11.84QAKK32 pKa = 10.2KK33 pKa = 9.9EE34 pKa = 4.02VALQAVHH41 pKa = 6.89KK42 pKa = 10.51ANKK45 pKa = 9.23HH46 pKa = 5.83DD47 pKa = 3.83EE48 pKa = 4.29AVSGGSFVFVADD60 pKa = 3.76KK61 pKa = 11.53VEE63 pKa = 3.94VTININFF70 pKa = 3.67

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9IR80|Q9IR80_9TOMB Putative movement protein P9 OS=Hibiscus chlorotic ringspot virus OX=53181 PE=4 SV=1
MM1 pKa = 6.95TQPFNVRR8 pKa = 11.84LMPIYY13 pKa = 10.44LGQSNLRR20 pKa = 11.84TMGGLLFLRR29 pKa = 11.84VKK31 pKa = 10.53RR32 pKa = 11.84EE33 pKa = 3.73QPIDD37 pKa = 3.57TRR39 pKa = 11.84VEE41 pKa = 3.96PVLLLLKK48 pKa = 10.19LSCQHH53 pKa = 6.73LRR55 pKa = 11.84LQLRR59 pKa = 11.84WPPRR63 pKa = 11.84CGLEE67 pKa = 4.09TLEE70 pKa = 4.1QTLGRR75 pKa = 11.84LEE77 pKa = 3.98NRR79 pKa = 11.84SLSSIASLWAISPGRR94 pKa = 11.84LVVGYY99 pKa = 8.31WWSRR103 pKa = 11.84GQSTPATYY111 pKa = 10.26SASPGFLYY119 pKa = 10.75LLPDD123 pKa = 3.6MRR125 pKa = 11.84STACHH130 pKa = 6.57HH131 pKa = 6.17YY132 pKa = 10.81LFDD135 pKa = 4.11ILQLAPQQRR144 pKa = 11.84RR145 pKa = 11.84ARR147 pKa = 11.84SSWHH151 pKa = 6.47LIKK154 pKa = 9.98MLQMLPLPPRR164 pKa = 11.84ATCITMMEE172 pKa = 4.19QLEE175 pKa = 4.47SRR177 pKa = 11.84PGIVPCCKK185 pKa = 9.75YY186 pKa = 10.56LAIMWIGLLMIPVVPTLSLLTLVRR210 pKa = 11.84WLLPIMANLKK220 pKa = 9.52GMWMM224 pKa = 4.03

Molecular weight: 25.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1875

70

735

267.9

29.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.533 ± 1.28

2.24 ± 0.322

3.84 ± 0.698

5.013 ± 1.091

4.053 ± 0.578

6.507 ± 0.59

2.72 ± 0.422

5.013 ± 0.226

5.067 ± 0.469

11.2 ± 2.12

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.449

3.2 ± 0.634

5.547 ± 0.528

3.093 ± 0.553

7.147 ± 0.736

7.573 ± 1.2

5.493 ± 0.943

6.933 ± 0.588

1.867 ± 0.328

2.4 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski