Alternaria brassicicola fusarivirus 1
Average proteome isoelectric point is 7.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U4D408|A0A0U4D408_9VIRU RNA-directed RNA polymerase OS=Alternaria brassicicola fusarivirus 1 OX=1766767 PE=4 SV=1
MM1 pKa = 7.01 MVKK4 pKa = 10.31 LVPSSRR10 pKa = 11.84 QKK12 pKa = 10.5 PEE14 pKa = 3.79 KK15 pKa = 10.41 EE16 pKa = 3.82 EE17 pKa = 4.08 LASSRR22 pKa = 11.84 QEE24 pKa = 4.1 QKK26 pKa = 10.47 CLSYY30 pKa = 11.14 VLSLSGTGFMSVQGGKK46 pKa = 7.39 PQCRR50 pKa = 11.84 RR51 pKa = 11.84 VEE53 pKa = 4.03 TCDD56 pKa = 3.54 NSEE59 pKa = 4.66 CNDD62 pKa = 3.55 CEE64 pKa = 4.56 LFVPVSS70 pKa = 3.38
Molecular weight: 7.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.654
IPC2_protein 6.046
IPC_protein 6.071
Toseland 5.677
ProMoST 6.097
Dawson 6.351
Bjellqvist 6.186
Wikipedia 6.211
Rodwell 6.3
Grimsley 5.588
Solomon 6.389
Lehninger 6.389
Nozaki 6.402
DTASelect 6.465
Thurlkill 6.376
EMBOSS 6.389
Sillero 6.664
Patrickios 4.024
IPC_peptide 6.402
IPC2_peptide 6.351
IPC2.peptide.svr19 6.477
Protein with the highest isoelectric point:
>tr|A0A0U4B4N9|A0A0U4B4N9_9VIRU Uncharacterized protein OS=Alternaria brassicicola fusarivirus 1 OX=1766767 PE=4 SV=1
MM1 pKa = 7.32 SANKK5 pKa = 9.68 IFEE8 pKa = 4.17 GKK10 pKa = 10.18 RR11 pKa = 11.84 IVKK14 pKa = 9.97 ARR16 pKa = 11.84 EE17 pKa = 3.52 QLASVEE23 pKa = 4.04 LTAQQVKK30 pKa = 10.47 SSTVWLVRR38 pKa = 11.84 EE39 pKa = 4.31 PGWIAGEE46 pKa = 4.08 EE47 pKa = 4.1 KK48 pKa = 10.97 LNGWFLEE55 pKa = 4.25 IQGSDD60 pKa = 2.43 IVLCVTTLFKK70 pKa = 11.58 GEE72 pKa = 4.18 VTSEE76 pKa = 3.5 RR77 pKa = 11.84 WFPPSPVFKK86 pKa = 10.0 WKK88 pKa = 10.39 KK89 pKa = 9.02 EE90 pKa = 3.85 YY91 pKa = 10.14 QSRR94 pKa = 11.84 DD95 pKa = 3.16 KK96 pKa = 11.09 EE97 pKa = 4.51 VASEE101 pKa = 3.8 EE102 pKa = 4.33 AYY104 pKa = 10.11 KK105 pKa = 10.64 KK106 pKa = 10.84 VIVARR111 pKa = 11.84 MADD114 pKa = 3.87 EE115 pKa = 4.52 NLLGYY120 pKa = 10.93 YY121 pKa = 10.95 DD122 pKa = 4.64 MLTPATPLVNRR133 pKa = 11.84 LSASFKK139 pKa = 8.0 WWKK142 pKa = 8.96 TGGNAPIIPFSPTNSKK158 pKa = 10.82 SEE160 pKa = 3.82 IVGRR164 pKa = 11.84 IAHH167 pKa = 6.61 FEE169 pKa = 3.99 TAFAALNNNPPFALDD184 pKa = 3.18 GLLALRR190 pKa = 11.84 VLKK193 pKa = 10.71 DD194 pKa = 3.27 QLPNMKK200 pKa = 10.27 DD201 pKa = 2.78 RR202 pKa = 11.84 GAQNVSRR209 pKa = 11.84 FLTTLVKK216 pKa = 10.09 AWAMSKK222 pKa = 10.14 QPSHH226 pKa = 7.01 FKK228 pKa = 11.08 GPLSKK233 pKa = 9.94 ISYY236 pKa = 7.0 PTEE239 pKa = 4.27 KK240 pKa = 10.35 EE241 pKa = 3.8 GLPADD246 pKa = 4.03 RR247 pKa = 11.84 AKK249 pKa = 10.49 IFHH252 pKa = 6.46 NLICGKK258 pKa = 10.15 GDD260 pKa = 3.8 EE261 pKa = 4.37 ITRR264 pKa = 11.84 GLGFCLWAPPRR275 pKa = 11.84 ITVTTDD281 pKa = 2.73 AEE283 pKa = 4.79 GFTSKK288 pKa = 9.24 QRR290 pKa = 11.84 KK291 pKa = 7.02 KK292 pKa = 10.2 HH293 pKa = 4.67 EE294 pKa = 4.32 EE295 pKa = 3.88 KK296 pKa = 10.76 KK297 pKa = 10.66 IMLDD301 pKa = 3.19 IGPDD305 pKa = 3.28 IKK307 pKa = 11.18 VGAALDD313 pKa = 3.9 AEE315 pKa = 4.25 PLEE318 pKa = 4.6 GSFAEE323 pKa = 4.66 SSAAGASRR331 pKa = 11.84 SHH333 pKa = 5.91 MKK335 pKa = 10.52 KK336 pKa = 10.5 NSDD339 pKa = 3.49 PEE341 pKa = 4.33 ANVLKK346 pKa = 10.66 EE347 pKa = 4.36 SGPKK351 pKa = 8.88 PDD353 pKa = 3.63 NVQEE357 pKa = 4.17 EE358 pKa = 4.98 VVDD361 pKa = 5.28 EE362 pKa = 4.34 AADD365 pKa = 3.44 WLKK368 pKa = 11.44 NSFTMVNFRR377 pKa = 11.84 RR378 pKa = 11.84 FYY380 pKa = 10.36 RR381 pKa = 11.84 RR382 pKa = 11.84 VKK384 pKa = 10.63 RR385 pKa = 11.84 SVTTIKK391 pKa = 10.68 EE392 pKa = 4.17 SVTYY396 pKa = 10.04 EE397 pKa = 3.7 GVGINRR403 pKa = 11.84 PFMEE407 pKa = 4.43 KK408 pKa = 10.55 AKK410 pKa = 8.77 NTVVFTVTSAVKK422 pKa = 10.28 ACATVPIAAAMGVWYY437 pKa = 9.82 GVKK440 pKa = 10.27 EE441 pKa = 4.04 PFVSIKK447 pKa = 10.64 DD448 pKa = 3.64 FPTPSGKK455 pKa = 9.77 VEE457 pKa = 4.15 MAKK460 pKa = 10.2 HH461 pKa = 5.11 VADD464 pKa = 3.75 CAVYY468 pKa = 10.33 SVTSVVTSTISGIFAGLASATRR490 pKa = 3.68
Molecular weight: 54.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.78
IPC2_protein 8.551
IPC_protein 8.419
Toseland 9.414
ProMoST 9.092
Dawson 9.604
Bjellqvist 9.297
Wikipedia 9.721
Rodwell 10.087
Grimsley 9.633
Solomon 9.648
Lehninger 9.619
Nozaki 9.531
DTASelect 9.238
Thurlkill 9.487
EMBOSS 9.823
Sillero 9.56
Patrickios 5.931
IPC_peptide 9.648
IPC2_peptide 7.761
IPC2.peptide.svr19 7.868
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2082
70
1522
694.0
78.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.916 ± 1.404
0.865 ± 1.023
4.803 ± 0.606
6.148 ± 0.874
5.091 ± 0.392
5.812 ± 0.241
1.873 ± 0.486
5.331 ± 0.928
7.301 ± 0.793
9.75 ± 1.597
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.594 ± 0.274
3.65 ± 0.141
4.947 ± 0.417
2.257 ± 0.755
5.764 ± 0.512
7.349 ± 1.173
6.34 ± 0.556
7.973 ± 0.487
2.354 ± 0.392
2.882 ± 0.605
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here