Siegesbeckia yellow vein betasatellite
Average proteome isoelectric point is 5.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5ZFG0|U5ZFG0_9VIRU Pathogenicity beta C1 protein OS=Siegesbeckia yellow vein betasatellite OX=1418029 GN=bC1 PE=4 SV=1
MM1 pKa = 7.65 TITYY5 pKa = 10.31 KK6 pKa = 10.6 NGKK9 pKa = 9.28 GVTFTINVRR18 pKa = 11.84 LSPNLKK24 pKa = 8.96 VHH26 pKa = 7.41 LSMLCTNEE34 pKa = 4.01 PVMSRR39 pKa = 11.84 YY40 pKa = 8.47 TYY42 pKa = 8.2 TLPYY46 pKa = 8.67 EE47 pKa = 4.64 HH48 pKa = 7.59 EE49 pKa = 5.34 DD50 pKa = 3.39 IIPPFDD56 pKa = 3.58 INGTEE61 pKa = 4.07 EE62 pKa = 4.08 AVKK65 pKa = 9.0 EE66 pKa = 4.19 TLRR69 pKa = 11.84 IMTEE73 pKa = 3.66 DD74 pKa = 4.14 VYY76 pKa = 11.39 FKK78 pKa = 10.64 DD79 pKa = 3.09 ITKK82 pKa = 10.82 EE83 pKa = 3.95 EE84 pKa = 4.18 VLDD87 pKa = 5.03 SIDD90 pKa = 2.84 TMMIEE95 pKa = 3.74 RR96 pKa = 11.84 FHH98 pKa = 8.36 FIEE101 pKa = 5.58 LDD103 pKa = 3.67 TKK105 pKa = 10.94 GVTSVRR111 pKa = 11.84 SRR113 pKa = 11.84 CTLL116 pKa = 3.21
Molecular weight: 13.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.05
IPC2_protein 5.156
IPC_protein 5.029
Toseland 4.978
ProMoST 5.169
Dawson 5.016
Bjellqvist 5.143
Wikipedia 4.876
Rodwell 4.94
Grimsley 4.914
Solomon 5.016
Lehninger 4.978
Nozaki 5.143
DTASelect 5.27
Thurlkill 4.978
EMBOSS 4.927
Sillero 5.219
Patrickios 3.961
IPC_peptide 5.029
IPC2_peptide 5.219
IPC2.peptide.svr19 5.188
Protein with the highest isoelectric point:
>tr|U5ZFG0|U5ZFG0_9VIRU Pathogenicity beta C1 protein OS=Siegesbeckia yellow vein betasatellite OX=1418029 GN=bC1 PE=4 SV=1
MM1 pKa = 7.65 TITYY5 pKa = 10.31 KK6 pKa = 10.6 NGKK9 pKa = 9.28 GVTFTINVRR18 pKa = 11.84 LSPNLKK24 pKa = 8.96 VHH26 pKa = 7.41 LSMLCTNEE34 pKa = 4.01 PVMSRR39 pKa = 11.84 YY40 pKa = 8.47 TYY42 pKa = 8.2 TLPYY46 pKa = 8.67 EE47 pKa = 4.64 HH48 pKa = 7.59 EE49 pKa = 5.34 DD50 pKa = 3.39 IIPPFDD56 pKa = 3.58 INGTEE61 pKa = 4.07 EE62 pKa = 4.08 AVKK65 pKa = 9.0 EE66 pKa = 4.19 TLRR69 pKa = 11.84 IMTEE73 pKa = 3.66 DD74 pKa = 4.14 VYY76 pKa = 11.39 FKK78 pKa = 10.64 DD79 pKa = 3.09 ITKK82 pKa = 10.82 EE83 pKa = 3.95 EE84 pKa = 4.18 VLDD87 pKa = 5.03 SIDD90 pKa = 2.84 TMMIEE95 pKa = 3.74 RR96 pKa = 11.84 FHH98 pKa = 8.36 FIEE101 pKa = 5.58 LDD103 pKa = 3.67 TKK105 pKa = 10.94 GVTSVRR111 pKa = 11.84 SRR113 pKa = 11.84 CTLL116 pKa = 3.21
Molecular weight: 13.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.05
IPC2_protein 5.156
IPC_protein 5.029
Toseland 4.978
ProMoST 5.169
Dawson 5.016
Bjellqvist 5.143
Wikipedia 4.876
Rodwell 4.94
Grimsley 4.914
Solomon 5.016
Lehninger 4.978
Nozaki 5.143
DTASelect 5.27
Thurlkill 4.978
EMBOSS 4.927
Sillero 5.219
Patrickios 3.961
IPC_peptide 5.029
IPC2_peptide 5.219
IPC2.peptide.svr19 5.188
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
116
116
116
116.0
13.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
0.862 ± 0.0
1.724 ± 0.0
6.034 ± 0.0
9.483 ± 0.0
4.31 ± 0.0
3.448 ± 0.0
2.586 ± 0.0
8.621 ± 0.0
6.034 ± 0.0
7.759 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.172 ± 0.0
4.31 ± 0.0
4.31 ± 0.0
0.0 ± 0.0
5.172 ± 0.0
5.172 ± 0.0
12.931 ± 0.0
7.759 ± 0.0
0.0 ± 0.0
4.31 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here