Altererythrobacter sp. FM1
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3033 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N2QFE4|A0A3N2QFE4_9SPHN TetR/AcrR family transcriptional regulator OS=Altererythrobacter sp. FM1 OX=2484537 GN=EB810_12525 PE=4 SV=1
MM1 pKa = 6.98 LTSIRR6 pKa = 11.84 SLAAATILSSSALIAAPALASDD28 pKa = 3.63 NGGAIVDD35 pKa = 3.83 TSVDD39 pKa = 3.6 VSSLDD44 pKa = 3.86 SVSLNATPAATGAIGAISFDD64 pKa = 3.6 SDD66 pKa = 3.37 ALSSVDD72 pKa = 3.34 TAASLEE78 pKa = 4.19 TSGAVIRR85 pKa = 11.84 TSQPVEE91 pKa = 4.13 DD92 pKa = 4.09 SNKK95 pKa = 10.4 AGEE98 pKa = 4.3 TGLTVSGSVTLVTDD112 pKa = 3.19 YY113 pKa = 11.07 RR114 pKa = 11.84 FRR116 pKa = 11.84 GASLSAGDD124 pKa = 4.17 PAIQGGITVTHH135 pKa = 7.25 DD136 pKa = 2.84 SGVYY140 pKa = 8.71 VGTWASSLAGGASYY154 pKa = 11.73 GNMEE158 pKa = 5.36 LDD160 pKa = 6.03 LIAGWSGDD168 pKa = 3.43 VSDD171 pKa = 5.75 GVTFDD176 pKa = 2.64 IGMVYY181 pKa = 9.96 FVYY184 pKa = 8.83 PTNDD188 pKa = 3.19 GAFDD192 pKa = 3.85 PVDD195 pKa = 3.34 YY196 pKa = 9.84 WEE198 pKa = 5.29 PYY200 pKa = 8.27 ATVSTTLGPVEE211 pKa = 4.21 ASLGINYY218 pKa = 9.19 AWKK221 pKa = 9.97 QDD223 pKa = 3.78 SLGGNDD229 pKa = 3.37 NLYY232 pKa = 10.92 LHH234 pKa = 7.21 GEE236 pKa = 4.17 LGVGIPNTPVSLSAHH251 pKa = 6.64 LGYY254 pKa = 9.52 TSGALAPDD262 pKa = 3.78 YY263 pKa = 10.08 TAGLSTDD270 pKa = 2.85 KK271 pKa = 10.68 TAWDD275 pKa = 3.57 WSIGASMTVLDD286 pKa = 4.29 KK287 pKa = 11.84 LEE289 pKa = 4.78 IGVSYY294 pKa = 10.5 IGVEE298 pKa = 4.08 APQAKK303 pKa = 10.21 ALGVDD308 pKa = 4.12 DD309 pKa = 5.51 GFTDD313 pKa = 4.03 GTIVGTLGVSFF324 pKa = 4.94
Molecular weight: 32.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.656
IPC_protein 3.681
Toseland 3.452
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.363
Solomon 3.694
Lehninger 3.643
Nozaki 3.808
DTASelect 4.101
Thurlkill 3.516
EMBOSS 3.668
Sillero 3.808
Patrickios 0.807
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|A0A3N2QGI9|A0A3N2QGI9_9SPHN Beta-lactamase OS=Altererythrobacter sp. FM1 OX=2484537 GN=EB810_11315 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 7.44 ATPGGRR28 pKa = 11.84 NVLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.02 KK41 pKa = 10.61 LSAA44 pKa = 4.03
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3033
0
3033
983523
29
4403
324.3
35.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.777 ± 0.064
0.822 ± 0.014
6.208 ± 0.039
5.713 ± 0.038
3.528 ± 0.027
8.766 ± 0.051
1.975 ± 0.025
5.097 ± 0.03
3.203 ± 0.037
9.632 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.465 ± 0.023
2.736 ± 0.034
5.165 ± 0.036
3.233 ± 0.026
6.991 ± 0.057
5.571 ± 0.038
5.325 ± 0.048
7.067 ± 0.037
1.433 ± 0.022
2.292 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here