Mycobacterium virus Bron
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 123 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E0YPN3|E0YPN3_9CAUD Uncharacterized protein OS=Mycobacterium virus Bron OX=861047 GN=100 PE=4 SV=1
MM1 pKa = 7.88 IEE3 pKa = 4.38 VYY5 pKa = 10.65 NSDD8 pKa = 2.83 MDD10 pKa = 4.13 ARR12 pKa = 11.84 CSVCDD17 pKa = 3.77 DD18 pKa = 4.02 MIRR21 pKa = 11.84 RR22 pKa = 11.84 GDD24 pKa = 3.76 EE25 pKa = 3.9 VVEE28 pKa = 4.06 FAGEE32 pKa = 4.01 LFHH35 pKa = 7.6 AEE37 pKa = 4.36 CVV39 pKa = 3.53
Molecular weight: 4.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.836
IPC2_protein 4.113
IPC_protein 3.961
Toseland 3.783
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.872
Rodwell 3.795
Grimsley 3.706
Solomon 3.923
Lehninger 3.872
Nozaki 4.075
DTASelect 4.24
Thurlkill 3.846
EMBOSS 3.884
Sillero 4.075
Patrickios 1.926
IPC_peptide 3.923
IPC2_peptide 4.062
IPC2.peptide.svr19 3.971
Protein with the highest isoelectric point:
>tr|E0YPH1|E0YPH1_9CAUD Uncharacterized protein OS=Mycobacterium virus Bron OX=861047 GN=38 PE=4 SV=1
MM1 pKa = 7.09 HH2 pKa = 6.87 TTHH5 pKa = 6.68 SVLDD9 pKa = 3.46 HH10 pKa = 5.25 TQIVDD15 pKa = 3.56 SALLGTVLAAGCTALYY31 pKa = 9.82 IRR33 pKa = 11.84 RR34 pKa = 11.84 HH35 pKa = 4.23 TWLVRR40 pKa = 11.84 WEE42 pKa = 4.23 TAATACIALLCVGTLCLSPQLIVFNPRR69 pKa = 11.84 LSPEE73 pKa = 3.64 IRR75 pKa = 11.84 TDD77 pKa = 3.41 ALPGQFLIGYY87 pKa = 7.59 LTLLLAAQMFLLDD100 pKa = 3.61 LAGRR104 pKa = 11.84 MVWEE108 pKa = 4.68 CAAKK112 pKa = 9.92 ARR114 pKa = 11.84 YY115 pKa = 7.76 MNLYY119 pKa = 9.51 VVLPGTIMLPTVIGLWTAQSRR140 pKa = 11.84 WVMEE144 pKa = 3.81 ILAVSLIYY152 pKa = 9.91 TLSHH156 pKa = 6.2 IGYY159 pKa = 9.01 LLWRR163 pKa = 11.84 IKK165 pKa = 10.14 RR166 pKa = 11.84 TDD168 pKa = 3.37 PRR170 pKa = 11.84 SGIVVDD176 pKa = 3.59 LHH178 pKa = 6.42 LAAFVLQVAAPLTRR192 pKa = 11.84 WFAPVSLWEE201 pKa = 3.8 PWGWRR206 pKa = 11.84 FRR208 pKa = 11.84 SFAALLFMAAASISWIRR225 pKa = 11.84 KK226 pKa = 6.94 RR227 pKa = 11.84 RR228 pKa = 11.84 YY229 pKa = 9.09 IKK231 pKa = 10.11 LHH233 pKa = 3.83 MWQRR237 pKa = 11.84 IRR239 pKa = 11.84 AEE241 pKa = 4.08 KK242 pKa = 9.08 PLKK245 pKa = 10.29 RR246 pKa = 11.84 KK247 pKa = 7.19 TSRR250 pKa = 11.84 RR251 pKa = 11.84 EE252 pKa = 3.64 HH253 pKa = 6.2 RR254 pKa = 11.84 RR255 pKa = 11.84 HH256 pKa = 5.14 KK257 pKa = 8.95 TAHH260 pKa = 5.7
Molecular weight: 29.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.809
IPC_protein 10.789
Toseland 10.73
ProMoST 10.57
Dawson 10.847
Bjellqvist 10.628
Wikipedia 11.111
Rodwell 10.935
Grimsley 10.906
Solomon 11.008
Lehninger 10.95
Nozaki 10.745
DTASelect 10.613
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.789
Patrickios 10.628
IPC_peptide 11.008
IPC2_peptide 9.897
IPC2.peptide.svr19 8.398
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
123
0
123
22305
29
1654
181.3
20.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.33 ± 0.381
1.161 ± 0.162
6.174 ± 0.168
6.362 ± 0.292
3.098 ± 0.118
8.258 ± 0.36
2.009 ± 0.16
4.34 ± 0.149
5.142 ± 0.244
8.456 ± 0.255
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.349 ± 0.145
3.735 ± 0.167
5.245 ± 0.167
3.421 ± 0.2
6.505 ± 0.278
5.425 ± 0.208
5.752 ± 0.251
7.097 ± 0.216
2.107 ± 0.157
3.035 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here