Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3085 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9RZT0|Q9RZT0_DEIRA Arylesterase/monoxygenase OS=Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) OX=243230 GN=DR_B0033 PE=3 SV=1
MM1 pKa = 7.85 DD2 pKa = 5.29 LSEE5 pKa = 4.12 LAHH8 pKa = 6.9 RR9 pKa = 11.84 ITYY12 pKa = 9.63 RR13 pKa = 11.84 AYY15 pKa = 10.05 EE16 pKa = 4.16 LDD18 pKa = 4.33 GDD20 pKa = 4.81 DD21 pKa = 5.1 LDD23 pKa = 5.42 SLAGLCGLMSWHH35 pKa = 6.33 TLIAPLTFQEE45 pKa = 5.14 FGTEE49 pKa = 3.9 DD50 pKa = 3.93 GRR52 pKa = 11.84 TLLCAADD59 pKa = 4.85 EE60 pKa = 4.47 SGLWITLTDD69 pKa = 3.98 GAAGVPTSPDD79 pKa = 3.11 TFQLSLAEE87 pKa = 4.81 DD88 pKa = 4.08 LLSEE92 pKa = 4.17 PVYY95 pKa = 10.53 TLDD98 pKa = 3.25 VVNGHH103 pKa = 5.84 VVQTAPGLNN112 pKa = 3.47
Molecular weight: 12.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.808
IPC_protein 3.757
Toseland 3.554
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.592
Grimsley 3.465
Solomon 3.745
Lehninger 3.694
Nozaki 3.884
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.719
Sillero 3.884
Patrickios 0.846
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|Q9RWZ6|Q9RWZ6_DEIRA Uncharacterized protein OS=Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) OX=243230 GN=DR_0519 PE=4 SV=1
MM1 pKa = 7.58 LCGGTRR7 pKa = 11.84 GVVSKK12 pKa = 11.03 AVGPFPGQGGEE23 pKa = 4.01 QARR26 pKa = 11.84 QRR28 pKa = 11.84 GPQRR32 pKa = 11.84 QGGSEE37 pKa = 4.33 MVWEE41 pKa = 4.49 TLPEE45 pKa = 4.18 AASLFACSSPGAKK58 pKa = 9.73 AGRR61 pKa = 11.84 TARR64 pKa = 11.84 QHH66 pKa = 4.98 STLARR71 pKa = 11.84 ALAAASPSDD80 pKa = 3.53 RR81 pKa = 11.84 TCTVLSQKK89 pKa = 10.49 AQARR93 pKa = 11.84 PAGQNFRR100 pKa = 11.84 WGPTQGNVKK109 pKa = 9.35 PRR111 pKa = 11.84 KK112 pKa = 8.39 RR113 pKa = 11.84 NVRR116 pKa = 11.84 NFRR119 pKa = 11.84 LLYY122 pKa = 8.44 GQAGAGIPVTANAISRR138 pKa = 11.84 QRR140 pKa = 11.84 GTIHH144 pKa = 6.49 TLSAAPNHH152 pKa = 6.55 RR153 pKa = 11.84 RR154 pKa = 11.84 FTMKK158 pKa = 9.45 NTTQGFTLIEE168 pKa = 4.17 LLIVIAIIGILAAVLIPNLLGARR191 pKa = 11.84 AKK193 pKa = 10.99 ANDD196 pKa = 3.96 SATQSYY202 pKa = 9.98 IRR204 pKa = 11.84 NCYY207 pKa = 8.03 TAVEE211 pKa = 4.5 VARR214 pKa = 11.84 GADD217 pKa = 3.33 GRR219 pKa = 11.84 LPAAGNCDD227 pKa = 3.02 STANLGTNAVATPGAITAAGAYY249 pKa = 9.94 SLDD252 pKa = 3.44 GTGSKK257 pKa = 10.3 FAVTATSVTNKK268 pKa = 8.3 TFCFDD273 pKa = 3.43 GAKK276 pKa = 9.61 MGEE279 pKa = 4.29 GTGATGACTFSTSTGTGTGTGTGTGTGTGTGTGSNN314 pKa = 3.8
Molecular weight: 31.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.589
IPC_protein 10.321
Toseland 10.687
ProMoST 10.365
Dawson 10.774
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.979
Grimsley 10.818
Solomon 10.891
Lehninger 10.862
Nozaki 10.701
DTASelect 10.467
Thurlkill 10.687
EMBOSS 11.082
Sillero 10.716
Patrickios 10.701
IPC_peptide 10.891
IPC2_peptide 9.75
IPC2.peptide.svr19 8.554
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3085
0
3085
949308
37
1940
307.7
33.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.205 ± 0.064
0.668 ± 0.013
5.069 ± 0.035
5.731 ± 0.046
3.156 ± 0.027
9.198 ± 0.053
2.085 ± 0.025
3.284 ± 0.037
2.717 ± 0.041
11.65 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.891 ± 0.023
2.407 ± 0.027
6.049 ± 0.041
4.118 ± 0.035
7.375 ± 0.042
5.2 ± 0.031
5.815 ± 0.041
7.687 ± 0.035
1.388 ± 0.017
2.303 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here