Prevotella bryantii B14

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella bryantii

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2748 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8DV14|D8DV14_PREBR Putative lipoprotein OS=Prevotella bryantii B14 OX=752555 GN=PBR_0386 PE=4 SV=1
HHH2 pKa = 7.04LFWPIPNSAITANNQGQLRR21 pKa = 11.84QNYYY25 pKa = 9.45YYY27 pKa = 10.57DD28 pKa = 3.77YYY30 pKa = 11.62DDD32 pKa = 6.04IPMWTNWEEE41 pKa = 3.99DD42 pKa = 3.68VADDD46 pKa = 4.08EE47 pKa = 4.42

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8DU91|D8DU91_PREBR Transcriptional regulator MraZ OS=Prevotella bryantii B14 OX=752555 GN=mraZ PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 9.6RR3 pKa = 11.84TFQPHH8 pKa = 3.81NRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84VNKK15 pKa = 8.88HH16 pKa = 4.1GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.97NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.44GRR39 pKa = 11.84KK40 pKa = 9.33KK41 pKa = 9.62LTVSSEE47 pKa = 4.03HH48 pKa = 6.64HH49 pKa = 6.27GKK51 pKa = 10.17

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2748

0

2748

1019909

30

4092

371.1

41.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.902 ± 0.037

1.228 ± 0.017

5.891 ± 0.029

6.007 ± 0.044

4.363 ± 0.03

6.766 ± 0.048

2.182 ± 0.022

7.279 ± 0.051

6.911 ± 0.041

8.589 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.749 ± 0.022

5.616 ± 0.052

3.453 ± 0.024

3.734 ± 0.025

4.164 ± 0.03

6.279 ± 0.044

5.761 ± 0.048

6.2 ± 0.035

1.313 ± 0.022

4.611 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski