Amino acid dipepetide frequency for Clostridium sp. DSM 8431

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.967AlaAla: 3.967 ± 0.081
0.742AlaCys: 0.742 ± 0.028
2.897AlaAsp: 2.897 ± 0.055
3.761AlaGlu: 3.761 ± 0.067
2.401AlaPhe: 2.401 ± 0.048
3.752AlaGly: 3.752 ± 0.068
0.779AlaHis: 0.779 ± 0.025
5.103AlaIle: 5.103 ± 0.077
4.876AlaLys: 4.876 ± 0.073
5.8AlaLeu: 5.8 ± 0.087
1.602AlaMet: 1.602 ± 0.044
2.53AlaAsn: 2.53 ± 0.054
1.392AlaPro: 1.392 ± 0.039
1.2AlaGln: 1.2 ± 0.033
1.8AlaArg: 1.8 ± 0.046
3.521AlaSer: 3.521 ± 0.063
2.516AlaThr: 2.516 ± 0.051
4.202AlaVal: 4.202 ± 0.067
0.365AlaTrp: 0.365 ± 0.021
2.213AlaTyr: 2.213 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.679CysAla: 0.679 ± 0.026
0.214CysCys: 0.214 ± 0.017
0.727CysAsp: 0.727 ± 0.026
0.842CysGlu: 0.842 ± 0.027
0.551CysPhe: 0.551 ± 0.024
1.143CysGly: 1.143 ± 0.037
0.244CysHis: 0.244 ± 0.016
1.229CysIle: 1.229 ± 0.038
1.135CysLys: 1.135 ± 0.034
0.955CysLeu: 0.955 ± 0.029
0.284CysMet: 0.284 ± 0.019
0.812CysAsn: 0.812 ± 0.034
0.486CysPro: 0.486 ± 0.022
0.202CysGln: 0.202 ± 0.013
0.441CysArg: 0.441 ± 0.022
0.874CysSer: 0.874 ± 0.028
0.685CysThr: 0.685 ± 0.03
0.668CysVal: 0.668 ± 0.026
0.074CysTrp: 0.074 ± 0.009
0.514CysTyr: 0.514 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
2.761AspAla: 2.761 ± 0.059
0.637AspCys: 0.637 ± 0.025
3.077AspAsp: 3.077 ± 0.06
5.292AspGlu: 5.292 ± 0.08
2.959AspPhe: 2.959 ± 0.053
3.63AspGly: 3.63 ± 0.082
0.569AspHis: 0.569 ± 0.024
6.214AspIle: 6.214 ± 0.087
5.652AspLys: 5.652 ± 0.083
5.055AspLeu: 5.055 ± 0.083
1.653AspMet: 1.653 ± 0.039
3.862AspAsn: 3.862 ± 0.073
1.269AspPro: 1.269 ± 0.037
0.733AspGln: 0.733 ± 0.026
1.941AspArg: 1.941 ± 0.047
3.668AspSer: 3.668 ± 0.074
2.694AspThr: 2.694 ± 0.054
3.818AspVal: 3.818 ± 0.066
0.386AspTrp: 0.386 ± 0.018
2.931AspTyr: 2.931 ± 0.065
0.0AspXaa: 0.0 ± 0.0
Glu
4.5GluAla: 4.5 ± 0.081
0.862GluCys: 0.862 ± 0.031
5.044GluAsp: 5.044 ± 0.089
7.836GluGlu: 7.836 ± 0.103
3.271GluPhe: 3.271 ± 0.06
4.428GluGly: 4.428 ± 0.073
0.839GluHis: 0.839 ± 0.03
7.011GluIle: 7.011 ± 0.099
8.103GluLys: 8.103 ± 0.094
6.75GluLeu: 6.75 ± 0.088
1.944GluMet: 1.944 ± 0.044
5.432GluAsn: 5.432 ± 0.077
1.382GluPro: 1.382 ± 0.041
1.421GluGln: 1.421 ± 0.041
2.634GluArg: 2.634 ± 0.051
4.025GluSer: 4.025 ± 0.062
2.979GluThr: 2.979 ± 0.062
5.486GluVal: 5.486 ± 0.091
0.466GluTrp: 0.466 ± 0.022
3.364GluTyr: 3.364 ± 0.059
0.0GluXaa: 0.0 ± 0.0
Phe
2.328PheAla: 2.328 ± 0.054
0.564PheCys: 0.564 ± 0.023
2.723PheAsp: 2.723 ± 0.053
2.879PheGlu: 2.879 ± 0.055
1.997PhePhe: 1.997 ± 0.051
2.683PheGly: 2.683 ± 0.057
0.485PheHis: 0.485 ± 0.026
4.584PheIle: 4.584 ± 0.093
4.147PheLys: 4.147 ± 0.07
3.897PheLeu: 3.897 ± 0.086
1.133PheMet: 1.133 ± 0.034
3.043PheAsn: 3.043 ± 0.056
1.059PhePro: 1.059 ± 0.032
0.848PheGln: 0.848 ± 0.028
1.257PheArg: 1.257 ± 0.036
3.148PheSer: 3.148 ± 0.062
2.253PheThr: 2.253 ± 0.048
2.676PheVal: 2.676 ± 0.061
0.292PheTrp: 0.292 ± 0.02
1.897PheTyr: 1.897 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
3.802GlyAla: 3.802 ± 0.075
0.951GlyCys: 0.951 ± 0.033
3.354GlyAsp: 3.354 ± 0.066
4.51GlyGlu: 4.51 ± 0.062
2.835GlyPhe: 2.835 ± 0.061
4.012GlyGly: 4.012 ± 0.074
0.946GlyHis: 0.946 ± 0.032
6.275GlyIle: 6.275 ± 0.085
5.585GlyLys: 5.585 ± 0.075
5.005GlyLeu: 5.005 ± 0.078
1.721GlyMet: 1.721 ± 0.044
3.369GlyAsn: 3.369 ± 0.069
1.057GlyPro: 1.057 ± 0.03
1.399GlyGln: 1.399 ± 0.039
2.34GlyArg: 2.34 ± 0.047
3.685GlySer: 3.685 ± 0.062
3.364GlyThr: 3.364 ± 0.062
4.73GlyVal: 4.73 ± 0.074
0.493GlyTrp: 0.493 ± 0.027
3.042GlyTyr: 3.042 ± 0.062
0.0GlyXaa: 0.0 ± 0.0
His
0.641HisAla: 0.641 ± 0.025
0.216HisCys: 0.216 ± 0.016
0.7HisAsp: 0.7 ± 0.029
0.816HisGlu: 0.816 ± 0.029
0.614HisPhe: 0.614 ± 0.023
0.962HisGly: 0.962 ± 0.033
0.267HisHis: 0.267 ± 0.016
1.2HisIle: 1.2 ± 0.031
1.087HisLys: 1.087 ± 0.033
1.067HisLeu: 1.067 ± 0.033
0.304HisMet: 0.304 ± 0.016
0.828HisAsn: 0.828 ± 0.028
0.537HisPro: 0.537 ± 0.023
0.266HisGln: 0.266 ± 0.015
0.497HisArg: 0.497 ± 0.024
0.827HisSer: 0.827 ± 0.033
0.632HisThr: 0.632 ± 0.025
0.732HisVal: 0.732 ± 0.03
0.091HisTrp: 0.091 ± 0.009
0.558HisTyr: 0.558 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
5.403IleAla: 5.403 ± 0.079
1.314IleCys: 1.314 ± 0.034
5.922IleAsp: 5.922 ± 0.078
7.115IleGlu: 7.115 ± 0.096
4.145IlePhe: 4.145 ± 0.093
5.661IleGly: 5.661 ± 0.082
1.185IleHis: 1.185 ± 0.033
8.633IleIle: 8.633 ± 0.151
8.731IleLys: 8.731 ± 0.108
8.928IleLeu: 8.928 ± 0.126
2.308IleMet: 2.308 ± 0.05
6.616IleAsn: 6.616 ± 0.089
2.894IlePro: 2.894 ± 0.054
1.947IleGln: 1.947 ± 0.047
2.948IleArg: 2.948 ± 0.058
6.991IleSer: 6.991 ± 0.097
4.733IleThr: 4.733 ± 0.071
5.874IleVal: 5.874 ± 0.087
0.47IleTrp: 0.47 ± 0.028
3.628IleTyr: 3.628 ± 0.068
0.0IleXaa: 0.0 ± 0.0
Lys
4.984LysAla: 4.984 ± 0.081
1.059LysCys: 1.059 ± 0.038
6.48LysAsp: 6.48 ± 0.092
9.581LysGlu: 9.581 ± 0.119
3.431LysPhe: 3.431 ± 0.064
5.171LysGly: 5.171 ± 0.07
1.119LysHis: 1.119 ± 0.035
8.232LysIle: 8.232 ± 0.1
8.823LysLys: 8.823 ± 0.112
7.66LysLeu: 7.66 ± 0.088
2.378LysMet: 2.378 ± 0.045
6.426LysAsn: 6.426 ± 0.087
1.977LysPro: 1.977 ± 0.047
1.873LysGln: 1.873 ± 0.044
3.293LysArg: 3.293 ± 0.057
5.49LysSer: 5.49 ± 0.081
4.12LysThr: 4.12 ± 0.072
6.501LysVal: 6.501 ± 0.087
0.637LysTrp: 0.637 ± 0.028
4.378LysTyr: 4.378 ± 0.07
0.0LysXaa: 0.0 ± 0.0
Leu
5.025LeuAla: 5.025 ± 0.092
1.172LeuCys: 1.172 ± 0.039
5.345LeuAsp: 5.345 ± 0.091
6.421LeuGlu: 6.421 ± 0.098
3.715LeuPhe: 3.715 ± 0.078
5.732LeuGly: 5.732 ± 0.086
1.059LeuHis: 1.059 ± 0.035
7.965LeuIle: 7.965 ± 0.105
8.847LeuLys: 8.847 ± 0.1
7.522LeuLeu: 7.522 ± 0.117
2.22LeuMet: 2.22 ± 0.045
6.016LeuAsn: 6.016 ± 0.075
2.418LeuPro: 2.418 ± 0.046
1.932LeuGln: 1.932 ± 0.047
3.142LeuArg: 3.142 ± 0.066
6.852LeuSer: 6.852 ± 0.088
4.302LeuThr: 4.302 ± 0.065
5.097LeuVal: 5.097 ± 0.078
0.553LeuTrp: 0.553 ± 0.026
3.278LeuTyr: 3.278 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
1.587MetAla: 1.587 ± 0.038
0.325MetCys: 0.325 ± 0.019
1.574MetAsp: 1.574 ± 0.033
1.713MetGlu: 1.713 ± 0.045
1.025MetPhe: 1.025 ± 0.029
1.704MetGly: 1.704 ± 0.046
0.363MetHis: 0.363 ± 0.021
2.287MetIle: 2.287 ± 0.045
2.635MetLys: 2.635 ± 0.05
2.133MetLeu: 2.133 ± 0.042
0.659MetMet: 0.659 ± 0.025
1.79MetAsn: 1.79 ± 0.046
0.896MetPro: 0.896 ± 0.033
0.646MetGln: 0.646 ± 0.024
0.85MetArg: 0.85 ± 0.031
1.664MetSer: 1.664 ± 0.041
1.132MetThr: 1.132 ± 0.031
1.469MetVal: 1.469 ± 0.037
0.168MetTrp: 0.168 ± 0.013
0.907MetTyr: 0.907 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
3.038AsnAla: 3.038 ± 0.06
0.721AsnCys: 0.721 ± 0.032
3.36AsnAsp: 3.36 ± 0.059
4.696AsnGlu: 4.696 ± 0.079
2.758AsnPhe: 2.758 ± 0.055
3.764AsnGly: 3.764 ± 0.075
0.793AsnHis: 0.793 ± 0.029
7.204AsnIle: 7.204 ± 0.1
6.404AsnLys: 6.404 ± 0.089
5.724AsnLeu: 5.724 ± 0.083
1.723AsnMet: 1.723 ± 0.044
4.891AsnAsn: 4.891 ± 0.096
1.815AsnPro: 1.815 ± 0.045
1.185AsnGln: 1.185 ± 0.033
1.994AsnArg: 1.994 ± 0.043
4.366AsnSer: 4.366 ± 0.072
3.067AsnThr: 3.067 ± 0.062
3.711AsnVal: 3.711 ± 0.068
0.423AsnTrp: 0.423 ± 0.021
2.941AsnTyr: 2.941 ± 0.062
0.0AsnXaa: 0.0 ± 0.0
Pro
1.294ProAla: 1.294 ± 0.035
0.354ProCys: 0.354 ± 0.018
1.412ProAsp: 1.412 ± 0.036
2.121ProGlu: 2.121 ± 0.05
1.199ProPhe: 1.199 ± 0.039
1.536ProGly: 1.536 ± 0.039
0.444ProHis: 0.444 ± 0.02
2.347ProIle: 2.347 ± 0.046
2.191ProLys: 2.191 ± 0.047
2.3ProLeu: 2.3 ± 0.057
0.648ProMet: 0.648 ± 0.026
1.307ProAsn: 1.307 ± 0.039
0.494ProPro: 0.494 ± 0.025
0.584ProGln: 0.584 ± 0.023
0.795ProArg: 0.795 ± 0.03
1.788ProSer: 1.788 ± 0.042
1.279ProThr: 1.279 ± 0.038
1.827ProVal: 1.827 ± 0.055
0.208ProTrp: 0.208 ± 0.015
1.222ProTyr: 1.222 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
1.178GlnAla: 1.178 ± 0.037
0.272GlnCys: 0.272 ± 0.014
1.125GlnAsp: 1.125 ± 0.035
1.496GlnGlu: 1.496 ± 0.04
0.854GlnPhe: 0.854 ± 0.029
1.364GlnGly: 1.364 ± 0.034
0.254GlnHis: 0.254 ± 0.016
1.84GlnIle: 1.84 ± 0.047
1.868GlnLys: 1.868 ± 0.047
1.662GlnLeu: 1.662 ± 0.044
0.567GlnMet: 0.567 ± 0.027
1.26GlnAsn: 1.26 ± 0.038
0.438GlnPro: 0.438 ± 0.02
0.505GlnGln: 0.505 ± 0.025
0.853GlnArg: 0.853 ± 0.031
1.214GlnSer: 1.214 ± 0.035
0.967GlnThr: 0.967 ± 0.033
1.437GlnVal: 1.437 ± 0.035
0.187GlnTrp: 0.187 ± 0.015
0.962GlnTyr: 0.962 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
1.782ArgAla: 1.782 ± 0.039
0.475ArgCys: 0.475 ± 0.022
2.002ArgAsp: 2.002 ± 0.041
2.975ArgGlu: 2.975 ± 0.054
1.469ArgPhe: 1.469 ± 0.038
2.038ArgGly: 2.038 ± 0.046
0.472ArgHis: 0.472 ± 0.022
3.115ArgIle: 3.115 ± 0.056
3.204ArgLys: 3.204 ± 0.06
2.745ArgLeu: 2.745 ± 0.063
0.89ArgMet: 0.89 ± 0.028
2.032ArgAsn: 2.032 ± 0.05
0.762ArgPro: 0.762 ± 0.029
0.832ArgGln: 0.832 ± 0.029
1.451ArgArg: 1.451 ± 0.046
1.599ArgSer: 1.599 ± 0.041
1.481ArgThr: 1.481 ± 0.037
2.302ArgVal: 2.302 ± 0.049
0.266ArgTrp: 0.266 ± 0.015
1.603ArgTyr: 1.603 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
3.302SerAla: 3.302 ± 0.063
0.756SerCys: 0.756 ± 0.027
3.594SerAsp: 3.594 ± 0.065
4.602SerGlu: 4.602 ± 0.08
3.22SerPhe: 3.22 ± 0.061
4.331SerGly: 4.331 ± 0.07
0.826SerHis: 0.826 ± 0.027
6.646SerIle: 6.646 ± 0.107
6.211SerLys: 6.211 ± 0.085
6.263SerLeu: 6.263 ± 0.095
1.656SerMet: 1.656 ± 0.042
4.228SerAsn: 4.228 ± 0.076
1.493SerPro: 1.493 ± 0.039
1.417SerGln: 1.417 ± 0.036
2.08SerArg: 2.08 ± 0.043
5.001SerSer: 5.001 ± 0.099
3.279SerThr: 3.279 ± 0.071
3.968SerVal: 3.968 ± 0.065
0.464SerTrp: 0.464 ± 0.023
2.973SerTyr: 2.973 ± 0.06
0.0SerXaa: 0.0 ± 0.0
Thr
3.04ThrAla: 3.04 ± 0.059
0.574ThrCys: 0.574 ± 0.025
2.471ThrAsp: 2.471 ± 0.063
3.105ThrGlu: 3.105 ± 0.065
2.187ThrPhe: 2.187 ± 0.053
3.627ThrGly: 3.627 ± 0.064
0.692ThrHis: 0.692 ± 0.027
4.501ThrIle: 4.501 ± 0.068
3.7ThrLys: 3.7 ± 0.061
4.668ThrLeu: 4.668 ± 0.065
1.095ThrMet: 1.095 ± 0.032
2.849ThrAsn: 2.849 ± 0.061
1.662ThrPro: 1.662 ± 0.048
0.93ThrGln: 0.93 ± 0.03
1.397ThrArg: 1.397 ± 0.037
3.342ThrSer: 3.342 ± 0.068
2.484ThrThr: 2.484 ± 0.06
3.255ThrVal: 3.255 ± 0.06
0.317ThrTrp: 0.317 ± 0.018
1.954ThrTyr: 1.954 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
3.742ValAla: 3.742 ± 0.068
0.843ValCys: 0.843 ± 0.031
3.846ValAsp: 3.846 ± 0.067
4.435ValGlu: 4.435 ± 0.068
2.94ValPhe: 2.94 ± 0.053
3.847ValGly: 3.847 ± 0.07
0.848ValHis: 0.848 ± 0.029
6.213ValIle: 6.213 ± 0.102
5.843ValLys: 5.843 ± 0.081
6.096ValLeu: 6.096 ± 0.093
1.559ValMet: 1.559 ± 0.039
3.928ValAsn: 3.928 ± 0.065
1.906ValPro: 1.906 ± 0.048
1.476ValGln: 1.476 ± 0.039
2.008ValArg: 2.008 ± 0.042
4.728ValSer: 4.728 ± 0.077
3.398ValThr: 3.398 ± 0.066
4.463ValVal: 4.463 ± 0.075
0.405ValTrp: 0.405 ± 0.023
2.569ValTyr: 2.569 ± 0.057
0.0ValXaa: 0.0 ± 0.0
Trp
0.346TrpAla: 0.346 ± 0.02
0.104TrpCys: 0.104 ± 0.011
0.415TrpAsp: 0.415 ± 0.022
0.381TrpGlu: 0.381 ± 0.02
0.291TrpPhe: 0.291 ± 0.019
0.473TrpGly: 0.473 ± 0.026
0.117TrpHis: 0.117 ± 0.01
0.648TrpIle: 0.648 ± 0.027
0.507TrpLys: 0.507 ± 0.025
0.516TrpLeu: 0.516 ± 0.025
0.198TrpMet: 0.198 ± 0.015
0.487TrpAsn: 0.487 ± 0.022
0.164TrpPro: 0.164 ± 0.013
0.201TrpGln: 0.201 ± 0.013
0.267TrpArg: 0.267 ± 0.014
0.463TrpSer: 0.463 ± 0.022
0.285TrpThr: 0.285 ± 0.018
0.41TrpVal: 0.41 ± 0.023
0.086TrpTrp: 0.086 ± 0.01
0.274TrpTyr: 0.274 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.021TyrAla: 2.021 ± 0.043
0.604TyrCys: 0.604 ± 0.024
2.735TyrAsp: 2.735 ± 0.065
3.198TyrGlu: 3.198 ± 0.059
2.031TyrPhe: 2.031 ± 0.054
2.619TyrGly: 2.619 ± 0.048
0.54TyrHis: 0.54 ± 0.022
4.199TyrIle: 4.199 ± 0.065
4.061TyrLys: 4.061 ± 0.07
3.865TyrLeu: 3.865 ± 0.068
0.981TyrMet: 0.981 ± 0.032
2.874TyrAsn: 2.874 ± 0.056
1.25TyrPro: 1.25 ± 0.036
0.708TyrGln: 0.708 ± 0.028
1.506TyrArg: 1.506 ± 0.041
3.066TyrSer: 3.066 ± 0.051
2.201TyrThr: 2.201 ± 0.054
2.466TyrVal: 2.466 ± 0.045
0.282TyrTrp: 0.282 ± 0.02
2.021TyrTyr: 2.021 ± 0.054
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3500 proteins (1035390 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski