Beihai toti-like virus 4
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KF81|A0A1L3KF81_9VIRU Uncharacterized protein OS=Beihai toti-like virus 4 OX=1922734 PE=4 SV=1
MM1 pKa = 7.43 GNAGAKK7 pKa = 10.29 GSGEE11 pKa = 4.31 GVGSAIGGALTGVSPMLPFPLNLVTGLGGTAANLVGKK48 pKa = 9.91 LVGDD52 pKa = 4.32 DD53 pKa = 3.34 PVVAQEE59 pKa = 4.4 AFQQILQQNPQLANQVQVLPPGPQVPPALHH89 pKa = 6.23 SQIQTTPSSVVTSGEE104 pKa = 4.0 TTPQSNQSGGVEE116 pKa = 3.98 GLLGHH121 pKa = 7.27 LKK123 pKa = 9.88 EE124 pKa = 4.29 ANKK127 pKa = 10.14 LQSFGPFTHH136 pKa = 7.42 APTQLNQQIDD146 pKa = 3.72 QLISHH151 pKa = 7.8 LGQGHH156 pKa = 5.59 LTKK159 pKa = 9.44 PTMAQVPTTSQIAPNTSIANKK180 pKa = 10.34 LGTTQLTADD189 pKa = 3.87 VAEE192 pKa = 4.0 QHH194 pKa = 5.26 YY195 pKa = 9.99 VKK197 pKa = 10.05 PIEE200 pKa = 4.26 SALVSGEE207 pKa = 3.81 AGKK210 pKa = 10.88 VGSTNPATNFALALQQGLFPQAQNTMKK237 pKa = 10.28 TLEE240 pKa = 4.19 NQNQEE245 pKa = 4.25 TNIPRR250 pKa = 11.84 PLMPGSQNQTEE261 pKa = 4.35 LTNPKK266 pKa = 10.16 VGTSTMLPLVPVNNARR282 pKa = 11.84 QLNLLNGMNTVGGEE296 pKa = 4.03 NNIVNSNLGTSTTSARR312 pKa = 11.84 PTSFGGILKK321 pKa = 8.87 DD322 pKa = 3.48 TALDD326 pKa = 3.73 VANVGAKK333 pKa = 9.85 EE334 pKa = 3.94 LGKK337 pKa = 8.49 TAGNFLRR344 pKa = 11.84 DD345 pKa = 4.0 RR346 pKa = 11.84 GPDD349 pKa = 3.29 MLKK352 pKa = 10.28 QGISFLGKK360 pKa = 8.17 TLGGLFRR367 pKa = 11.84 GKK369 pKa = 10.48 LVGDD373 pKa = 4.35 APLQPGTWSPGDD385 pKa = 3.88 FNLLPNTNEE394 pKa = 3.76 MFKK397 pKa = 10.49 PGAIGTDD404 pKa = 3.13 IVKK407 pKa = 9.87 MIGDD411 pKa = 3.96 APIDD415 pKa = 3.45 GVMSNDD421 pKa = 3.21 EE422 pKa = 3.64 ASAAVKK428 pKa = 10.81 AMTTSIAITLFAKK441 pKa = 10.35 LVIIEE446 pKa = 4.23 QTLEE450 pKa = 3.73 TALPIIFSNNIRR462 pKa = 11.84 QAAAQTARR470 pKa = 11.84 QITVQNGMVRR480 pKa = 11.84 LSQLLFNTDD489 pKa = 4.44 LIGNYY494 pKa = 9.32 YY495 pKa = 10.01 FYY497 pKa = 11.17 DD498 pKa = 5.09 GIPVYY503 pKa = 9.6 RR504 pKa = 11.84 TNHH507 pKa = 6.31 KK508 pKa = 9.41 DD509 pKa = 2.8 WHH511 pKa = 6.21 VEE513 pKa = 4.01 SPIYY517 pKa = 9.97 TPTYY521 pKa = 8.55 TGLVPISDD529 pKa = 4.32 SPPLLGNVTYY539 pKa = 10.31 EE540 pKa = 3.88 ALKK543 pKa = 10.19 PLLSGAPASEE553 pKa = 4.36 YY554 pKa = 10.37 PSTLRR559 pKa = 11.84 IKK561 pKa = 9.04 TRR563 pKa = 11.84 RR564 pKa = 11.84 TDD566 pKa = 2.81 IDD568 pKa = 3.5 ALLTISSQGTYY579 pKa = 10.36 EE580 pKa = 3.99 LTASAGYY587 pKa = 10.57 SFVCPLVKK595 pKa = 10.64 LLGYY599 pKa = 10.2 QSAIGQLPGDD609 pKa = 4.18 EE610 pKa = 4.23 MTQYY614 pKa = 10.46 MSGVYY619 pKa = 9.3 PVINFSQNPQPGATIFPWRR638 pKa = 11.84 SGSSDD643 pKa = 3.42 LVNHH647 pKa = 5.93 NTWKK651 pKa = 8.09 WLKK654 pKa = 9.54 VAWVTYY660 pKa = 8.98 TEE662 pKa = 4.1 FADD665 pKa = 4.86 AIVGMRR671 pKa = 11.84 APWLDD676 pKa = 3.13 VFQPINWGITCAVVFISRR694 pKa = 11.84 SEE696 pKa = 4.08 MQDD699 pKa = 2.54 GATNIVRR706 pKa = 11.84 MLSHH710 pKa = 6.77 IAYY713 pKa = 8.62 PFVIFPRR720 pKa = 11.84 EE721 pKa = 4.18 VKK723 pKa = 9.07 WVQAHH728 pKa = 6.3 KK729 pKa = 10.97 DD730 pKa = 3.19 EE731 pKa = 4.81 GFRR734 pKa = 11.84 EE735 pKa = 4.16 PVMNTTACWPHH746 pKa = 6.95 IFSAQIEE753 pKa = 4.77 GPDD756 pKa = 3.39 QAILFVVTDD765 pKa = 4.27 LSDD768 pKa = 3.45 RR769 pKa = 11.84 QAGFITLGIDD779 pKa = 3.25 NMVEE783 pKa = 3.92 VLTDD787 pKa = 4.0 VITPNTPWQLDD798 pKa = 3.88 EE799 pKa = 4.5 EE800 pKa = 4.53 QHH802 pKa = 4.85 EE803 pKa = 4.55 TRR805 pKa = 11.84 HH806 pKa = 6.26 PILMPIFSVINEE818 pKa = 4.06 PARR821 pKa = 11.84 FQAVIMSEE829 pKa = 3.36 IRR831 pKa = 11.84 RR832 pKa = 11.84 WEE834 pKa = 3.98 SYY836 pKa = 10.38 YY837 pKa = 11.37 GNNSDD842 pKa = 4.57 RR843 pKa = 11.84 SSAMRR848 pKa = 11.84 WCAEE852 pKa = 3.42 QSVRR856 pKa = 11.84 FGQPKK861 pKa = 9.77 NFCFTPTGTHH871 pKa = 6.74 NIYY874 pKa = 9.89 TGYY877 pKa = 9.18 TYY879 pKa = 11.07 NADD882 pKa = 3.67 PSVSPYY888 pKa = 11.26 GDD890 pKa = 3.4 LSKK893 pKa = 11.18 VFIMSEE899 pKa = 3.99 DD900 pKa = 3.4 GKK902 pKa = 10.61 KK903 pKa = 10.08 RR904 pKa = 11.84 WMFANMLSTTPLMITPNILDD924 pKa = 4.05 GYY926 pKa = 10.6 FRR928 pKa = 11.84 TPHH931 pKa = 6.05 NEE933 pKa = 3.57 YY934 pKa = 8.26 TTFATTHH941 pKa = 6.27 FLGQEE946 pKa = 3.98 DD947 pKa = 4.33 PVVNFLIEE955 pKa = 4.04 RR956 pKa = 11.84 GMVSPAEE963 pKa = 4.1 EE964 pKa = 4.48 YY965 pKa = 7.46 PTVRR969 pKa = 11.84 FTLAPKK975 pKa = 9.89 NYY977 pKa = 10.26 SAITEE982 pKa = 4.56 LAQLMAFIMDD992 pKa = 3.77 SSAQARR998 pKa = 11.84 SVTVKK1003 pKa = 10.56 DD1004 pKa = 3.79 YY1005 pKa = 11.55 YY1006 pKa = 11.06 SYY1008 pKa = 11.18 PNHH1011 pKa = 7.25 RR1012 pKa = 11.84 LNDD1015 pKa = 3.6 DD1016 pKa = 3.51 TNQNVVTRR1024 pKa = 11.84 MALEE1028 pKa = 4.14 KK1029 pKa = 10.75 VFFPVGHH1036 pKa = 6.58 AVCTNGIQKK1045 pKa = 10.38 PSFSVNYY1052 pKa = 9.67 QSLEE1056 pKa = 4.07 YY1057 pKa = 10.86 NHH1059 pKa = 6.75 TFPLYY1064 pKa = 10.47 FDD1066 pKa = 4.86 AVWSFLGDD1074 pKa = 3.37 GLTSAKK1080 pKa = 10.26 NSTVGVFGRR1089 pKa = 11.84 IPWALAEE1096 pKa = 4.07 RR1097 pKa = 11.84 WHH1099 pKa = 6.7 PSFGIRR1105 pKa = 11.84 DD1106 pKa = 3.95 TNTNVYY1112 pKa = 10.22 GLDD1115 pKa = 3.58 VKK1117 pKa = 9.97 ITEE1120 pKa = 4.24 HH1121 pKa = 6.11 MGVNGYY1127 pKa = 8.84 EE1128 pKa = 3.94 PHH1130 pKa = 6.64 EE1131 pKa = 5.14 LISMMSCDD1139 pKa = 3.81 YY1140 pKa = 10.79 DD1141 pKa = 3.84 VQFIASLFRR1150 pKa = 11.84 VSKK1153 pKa = 10.01 FLSGLMVSSLQVNNPLWLYY1172 pKa = 10.94 DD1173 pKa = 3.15 SSTNYY1178 pKa = 9.97 FYY1180 pKa = 11.59 YY1181 pKa = 10.79 LAGATLPLDD1190 pKa = 3.75 SVVGRR1195 pKa = 11.84 IIALSEE1201 pKa = 3.75 AVGVINIPNFNLTPLPICGFVPTYY1225 pKa = 10.24 NISGGGRR1232 pKa = 11.84 KK1233 pKa = 8.81 FRR1235 pKa = 11.84 FAAIQPVDD1243 pKa = 3.71 SFVNPSGSNDD1253 pKa = 3.31 YY1254 pKa = 11.15 QLFYY1258 pKa = 10.39 PVKK1261 pKa = 8.31 TQASLRR1267 pKa = 11.84 TTIGYY1272 pKa = 9.55 HH1273 pKa = 5.51 EE1274 pKa = 5.19 FGHH1277 pKa = 6.61 PLFSDD1282 pKa = 4.54 FTSNNPFAYY1291 pKa = 10.11
Molecular weight: 141.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.428
IPC2_protein 5.41
IPC_protein 5.436
Toseland 5.766
ProMoST 5.728
Dawson 5.614
Bjellqvist 5.664
Wikipedia 5.588
Rodwell 5.588
Grimsley 5.906
Solomon 5.614
Lehninger 5.601
Nozaki 5.842
DTASelect 6.033
Thurlkill 5.957
EMBOSS 5.919
Sillero 5.931
Patrickios 3.846
IPC_peptide 5.626
IPC2_peptide 5.944
IPC2.peptide.svr19 5.884
Protein with the highest isoelectric point:
>tr|A0A1L3KF81|A0A1L3KF81_9VIRU Uncharacterized protein OS=Beihai toti-like virus 4 OX=1922734 PE=4 SV=1
MM1 pKa = 7.82 AEE3 pKa = 3.9 NLKK6 pKa = 10.27 DD7 pKa = 3.68 IKK9 pKa = 10.91 VLYY12 pKa = 9.19 PDD14 pKa = 3.95 YY15 pKa = 11.49 EE16 pKa = 4.43 MTEE19 pKa = 4.3 SMWLDD24 pKa = 3.11 IVNIKK29 pKa = 10.14 LDD31 pKa = 3.94 KK32 pKa = 11.26 NDD34 pKa = 3.8 FLNDD38 pKa = 3.35 LTEE41 pKa = 4.62 DD42 pKa = 3.83 SNVPGISYY50 pKa = 9.93 FSSYY54 pKa = 9.84 HH55 pKa = 4.79 VNRR58 pKa = 11.84 IRR60 pKa = 11.84 QMPPMYY66 pKa = 10.44 FEE68 pKa = 5.16 QEE70 pKa = 4.1 FKK72 pKa = 11.01 NYY74 pKa = 9.92 PLTLKK79 pKa = 10.69 FCFSLLLQLHH89 pKa = 6.07 PVYY92 pKa = 10.41 RR93 pKa = 11.84 DD94 pKa = 3.3 IIISVLYY101 pKa = 10.26 AVNILPNCLTVTKK114 pKa = 10.49 YY115 pKa = 10.63 IEE117 pKa = 4.16 VCKK120 pKa = 10.77 KK121 pKa = 8.24 LTSWAKK127 pKa = 10.0 KK128 pKa = 9.74 CNSLASRR135 pKa = 11.84 LINNKK140 pKa = 9.09 FNCNVEE146 pKa = 4.3 DD147 pKa = 4.35 KK148 pKa = 10.84 FWMILVDD155 pKa = 3.37 WDD157 pKa = 3.54 IFSGYY162 pKa = 7.79 NFKK165 pKa = 11.22 YY166 pKa = 10.43 NEE168 pKa = 4.13 SKK170 pKa = 10.64 IIDD173 pKa = 4.21 SIKK176 pKa = 9.86 EE177 pKa = 3.83 WGSGDD182 pKa = 3.08 IYY184 pKa = 10.91 KK185 pKa = 10.96 GNNEE189 pKa = 3.98 VSSDD193 pKa = 3.32 KK194 pKa = 10.9 YY195 pKa = 9.7 YY196 pKa = 11.52 KK197 pKa = 10.89 LMEE200 pKa = 4.66 KK201 pKa = 10.58 YY202 pKa = 10.15 VDD204 pKa = 3.42 KK205 pKa = 11.01 LLEE208 pKa = 4.48 FGTLEE213 pKa = 4.09 KK214 pKa = 11.01 KK215 pKa = 10.69 NILSLRR221 pKa = 11.84 DD222 pKa = 3.55 WLSDD226 pKa = 3.08 PTKK229 pKa = 9.86 WVTAGSSNGVRR240 pKa = 11.84 VDD242 pKa = 2.96 IFSYY246 pKa = 10.84 DD247 pKa = 2.92 KK248 pKa = 11.11 NRR250 pKa = 11.84 NVKK253 pKa = 9.69 SAATKK258 pKa = 10.07 SVFSVKK264 pKa = 10.44 YY265 pKa = 10.63 DD266 pKa = 3.09 VDD268 pKa = 3.88 DD269 pKa = 4.4 LYY271 pKa = 11.38 KK272 pKa = 10.47 ILMTNTKK279 pKa = 9.43 PQDD282 pKa = 3.2 SRR284 pKa = 11.84 VNIKK288 pKa = 10.75 LEE290 pKa = 4.22 AGQKK294 pKa = 9.74 NRR296 pKa = 11.84 IIVSSGWVNQLRR308 pKa = 11.84 MAYY311 pKa = 9.3 ISSWLEE317 pKa = 3.51 KK318 pKa = 10.44 SLKK321 pKa = 9.16 NVPWTTLFSTNQDD334 pKa = 2.1 AWTRR338 pKa = 11.84 NVRR341 pKa = 11.84 KK342 pKa = 9.67 CSHH345 pKa = 6.47 IKK347 pKa = 10.15 SGSIMFPLDD356 pKa = 3.87 AKK358 pKa = 11.16 NFDD361 pKa = 3.73 QNVSKK366 pKa = 11.57 GEE368 pKa = 3.67 IDD370 pKa = 3.47 VCFNSIRR377 pKa = 11.84 KK378 pKa = 9.15 IINKK382 pKa = 9.54 SLAGEE387 pKa = 4.11 KK388 pKa = 10.5 RR389 pKa = 11.84 FDD391 pKa = 3.74 CLKK394 pKa = 10.9 LIDD397 pKa = 4.83 IIQNQFWNTFVVYY410 pKa = 10.87 KK411 pKa = 9.67 NMRR414 pKa = 11.84 MLWEE418 pKa = 4.66 HH419 pKa = 6.24 GMPSGIRR426 pKa = 11.84 WTAFLDD432 pKa = 3.66 TLINAARR439 pKa = 11.84 VCIINDD445 pKa = 3.37 ILSKK449 pKa = 11.0 GSTIPIKK456 pKa = 10.42 EE457 pKa = 3.91 YY458 pKa = 9.7 TAQGDD463 pKa = 3.86 DD464 pKa = 4.69 DD465 pKa = 4.7 DD466 pKa = 6.89 LEE468 pKa = 4.23 VSKK471 pKa = 10.67 WDD473 pKa = 4.66 DD474 pKa = 3.02 ILNIFNTYY482 pKa = 9.82 NSLGIVLAPQKK493 pKa = 10.87 NFVSTKK499 pKa = 5.83 MTEE502 pKa = 4.24 FLRR505 pKa = 11.84 KK506 pKa = 9.83 VYY508 pKa = 10.44 FSDD511 pKa = 5.82 LIMGYY516 pKa = 7.53 ITRR519 pKa = 11.84 KK520 pKa = 7.68 MVNVCFVNPEE530 pKa = 4.15 KK531 pKa = 11.19 NLVNYY536 pKa = 10.28 RR537 pKa = 11.84 DD538 pKa = 3.65 VDD540 pKa = 3.6 LHH542 pKa = 5.94 TIRR545 pKa = 11.84 DD546 pKa = 3.15 IWLEE550 pKa = 3.71 LMQRR554 pKa = 11.84 GGDD557 pKa = 3.72 FEE559 pKa = 4.64 YY560 pKa = 11.16 CEE562 pKa = 4.44 SKK564 pKa = 10.8 LIEE567 pKa = 4.98 HH568 pKa = 7.09 IALVLFNKK576 pKa = 10.07 LGKK579 pKa = 9.86 IEE581 pKa = 4.94 GYY583 pKa = 10.47 SKK585 pKa = 10.37 LTDD588 pKa = 3.08 RR589 pKa = 11.84 HH590 pKa = 5.42 LAIAKK595 pKa = 9.81 DD596 pKa = 3.95 VLSTPASLGGLGCLWFGTRR615 pKa = 11.84 TWRR618 pKa = 11.84 VLQEE622 pKa = 3.99 KK623 pKa = 10.13 RR624 pKa = 11.84 SEE626 pKa = 3.53 RR627 pKa = 11.84 HH628 pKa = 4.05 YY629 pKa = 10.64 QLRR632 pKa = 11.84 GPLGVWTKK640 pKa = 10.81 VLSATNSVTGNVRR653 pKa = 11.84 RR654 pKa = 11.84 EE655 pKa = 3.77 NEE657 pKa = 3.54 AVIRR661 pKa = 11.84 DD662 pKa = 4.25 KK663 pKa = 10.82 IVQTLIPKK671 pKa = 9.67 HH672 pKa = 5.54 EE673 pKa = 4.14 RR674 pKa = 11.84 VPRR677 pKa = 11.84 IIEE680 pKa = 4.27 CEE682 pKa = 3.77 LLEE685 pKa = 4.52 IPFSGLIQVPEE696 pKa = 4.17 ITPSLQRR703 pKa = 11.84 TLPVWRR709 pKa = 11.84 MRR711 pKa = 11.84 DD712 pKa = 3.49 DD713 pKa = 3.54 IGPVDD718 pKa = 4.19 VNCVLNACKK727 pKa = 10.25 EE728 pKa = 4.02 NRR730 pKa = 11.84 DD731 pKa = 3.34 WGLLRR736 pKa = 11.84 SITSEE741 pKa = 4.18 SVLGTFDD748 pKa = 4.02 NMLSSKK754 pKa = 10.27 HH755 pKa = 5.71 KK756 pKa = 10.56 RR757 pKa = 11.84 EE758 pKa = 4.34 LLITWVMDD766 pKa = 3.24 DD767 pKa = 3.36 WTVNAPSHH775 pKa = 5.98 RR776 pKa = 11.84 VWGKK780 pKa = 7.23 TALSAMFVGWKK791 pKa = 9.7 NYY793 pKa = 9.94 CINRR797 pKa = 11.84 WYY799 pKa = 10.12 EE800 pKa = 3.88 VNNKK804 pKa = 8.83 GRR806 pKa = 11.84 DD807 pKa = 3.9 YY808 pKa = 9.93 YY809 pKa = 11.17 TKK811 pKa = 10.37 LCVSSEE817 pKa = 4.22 YY818 pKa = 11.18 NFYY821 pKa = 11.02 KK822 pKa = 10.58 YY823 pKa = 11.18 VNTHH827 pKa = 5.02 EE828 pKa = 4.95 HH829 pKa = 5.53 PVVMRR834 pKa = 11.84 FF835 pKa = 3.16
Molecular weight: 97.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.769
IPC2_protein 8.039
IPC_protein 7.834
Toseland 7.717
ProMoST 8.434
Dawson 8.682
Bjellqvist 8.858
Wikipedia 8.668
Rodwell 8.756
Grimsley 7.732
Solomon 8.726
Lehninger 8.726
Nozaki 9.004
DTASelect 8.639
Thurlkill 8.726
EMBOSS 8.858
Sillero 8.99
Patrickios 4.406
IPC_peptide 8.726
IPC2_peptide 7.6
IPC2.peptide.svr19 7.894
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
2126
835
1291
1063.0
119.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.597 ± 1.408
1.176 ± 0.512
4.986 ± 0.811
4.421 ± 0.434
4.563 ± 0.292
6.585 ± 1.283
1.787 ± 0.137
6.397 ± 0.684
5.127 ± 1.797
8.984 ± 0.356
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.681 ± 0.028
6.726 ± 0.345
5.503 ± 1.424
4.045 ± 1.126
3.998 ± 0.686
7.291 ± 0.08
7.008 ± 1.107
7.338 ± 0.195
2.07 ± 0.622
3.716 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here