Hollyhock yellow vein mosaic Islamabad virus
Average proteome isoelectric point is 7.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G4DIL0|A0A0G4DIL0_9GEMI Replication enhancer OS=Hollyhock yellow vein mosaic Islamabad virus OX=1504732 GN=REn PE=3 SV=1
MM1 pKa = 7.42 PRR3 pKa = 11.84 AFKK6 pKa = 10.99 LNAKK10 pKa = 9.86 NYY12 pKa = 8.38 FLTYY16 pKa = 9.06 PKK18 pKa = 10.53 CSLTKK23 pKa = 10.72 EE24 pKa = 4.17 EE25 pKa = 4.86 TLSQLQNLHH34 pKa = 5.69 TPVNKK39 pKa = 10.11 KK40 pKa = 9.89 YY41 pKa = 10.82 IKK43 pKa = 9.68 ICRR46 pKa = 11.84 EE47 pKa = 3.53 LHH49 pKa = 6.26 EE50 pKa = 4.93 NGEE53 pKa = 4.28 PHH55 pKa = 6.66 LHH57 pKa = 6.22 VLIQFDD63 pKa = 4.6 GKK65 pKa = 8.92 YY66 pKa = 9.41 QCTNKK71 pKa = 10.06 RR72 pKa = 11.84 FFDD75 pKa = 3.89 LVSPTRR81 pKa = 11.84 SAHH84 pKa = 4.86 FHH86 pKa = 6.55 PNIQGAKK93 pKa = 9.25 SSSDD97 pKa = 3.11 VKK99 pKa = 11.17 SYY101 pKa = 11.26 LEE103 pKa = 4.24 KK104 pKa = 11.19 DD105 pKa = 3.24 GDD107 pKa = 3.91 TLDD110 pKa = 3.28 WGEE113 pKa = 3.86 FQIDD117 pKa = 3.21 GRR119 pKa = 11.84 SARR122 pKa = 11.84 GGQQSANDD130 pKa = 3.91 AYY132 pKa = 10.27 ATALNAGSKK141 pKa = 10.23 SEE143 pKa = 3.84 ALRR146 pKa = 11.84 VIKK149 pKa = 10.21 EE150 pKa = 4.18 LAPKK154 pKa = 10.3 DD155 pKa = 3.74 YY156 pKa = 11.2 VLQFHH161 pKa = 6.76 NLNANLDD168 pKa = 4.31 RR169 pKa = 11.84 IFTPPMEE176 pKa = 4.56 VYY178 pKa = 10.67 VSPFSSSSFDD188 pKa = 3.41 QVPEE192 pKa = 3.98 EE193 pKa = 4.13 LEE195 pKa = 3.58 EE196 pKa = 3.9 WAAEE200 pKa = 4.15 NVVSAAARR208 pKa = 11.84 PLRR211 pKa = 11.84 PISIVIEE218 pKa = 4.23 GDD220 pKa = 3.2 SRR222 pKa = 11.84 TGKK225 pKa = 8.55 TMWARR230 pKa = 11.84 ALGPHH235 pKa = 6.7 NYY237 pKa = 10.19 LCGHH241 pKa = 7.38 LDD243 pKa = 4.18 LSPKK247 pKa = 10.02 VYY249 pKa = 10.89 SNEE252 pKa = 3.02 AWYY255 pKa = 10.82 NVIDD259 pKa = 5.29 DD260 pKa = 4.41 VDD262 pKa = 3.61 PHH264 pKa = 5.85 YY265 pKa = 11.0 LKK267 pKa = 10.7 HH268 pKa = 6.2 FKK270 pKa = 10.7 EE271 pKa = 4.46 FMGAQRR277 pKa = 11.84 DD278 pKa = 3.81 WQSNTKK284 pKa = 9.6 YY285 pKa = 10.57 GKK287 pKa = 9.12 PVQIKK292 pKa = 10.41 GGIPTIFLCNPGPNSSYY309 pKa = 11.34 KK310 pKa = 10.62 EE311 pKa = 3.85 FLDD314 pKa = 3.79 EE315 pKa = 4.63 EE316 pKa = 4.37 KK317 pKa = 11.05 NAALKK322 pKa = 10.45 NWALKK327 pKa = 10.17 NATFITLEE335 pKa = 4.26 GPLYY339 pKa = 10.84 SGSNQSAAQASQEE352 pKa = 3.81 GDD354 pKa = 3.32 QASTCC359 pKa = 3.84
Molecular weight: 40.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.048
IPC2_protein 6.033
IPC_protein 6.122
Toseland 6.478
ProMoST 6.532
Dawson 6.453
Bjellqvist 6.427
Wikipedia 6.465
Rodwell 6.44
Grimsley 6.62
Solomon 6.453
Lehninger 6.453
Nozaki 6.722
DTASelect 6.898
Thurlkill 6.927
EMBOSS 6.898
Sillero 6.839
Patrickios 4.101
IPC_peptide 6.465
IPC2_peptide 6.707
IPC2.peptide.svr19 6.722
Protein with the highest isoelectric point:
>tr|A0A0G4DI24|A0A0G4DI24_9GEMI Protein V2 OS=Hollyhock yellow vein mosaic Islamabad virus OX=1504732 GN=V2 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.87 IIISTPASKK16 pKa = 10.3 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 2.99 SPYY27 pKa = 10.45 ASRR30 pKa = 11.84 AAVPIVRR37 pKa = 11.84 VTKK40 pKa = 10.49 ARR42 pKa = 11.84 AWANRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.87 PRR54 pKa = 11.84 MYY56 pKa = 10.68 RR57 pKa = 11.84 MYY59 pKa = 10.44 RR60 pKa = 11.84 SPDD63 pKa = 3.21 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.01 VQSFEE78 pKa = 4.1 SRR80 pKa = 11.84 HH81 pKa = 6.13 DD82 pKa = 3.26 IQHH85 pKa = 6.35 IGKK88 pKa = 8.97 VMCVSDD94 pKa = 3.5 VTRR97 pKa = 11.84 GIGLTHH103 pKa = 6.67 RR104 pKa = 11.84 VGKK107 pKa = 9.66 RR108 pKa = 11.84 FCVKK112 pKa = 9.94 SVYY115 pKa = 10.52 VLGKK119 pKa = 9.41 IWMDD123 pKa = 3.35 EE124 pKa = 4.04 NIKK127 pKa = 9.32 TKK129 pKa = 10.6 NHH131 pKa = 5.78 TNSVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.84 RR143 pKa = 11.84 RR144 pKa = 11.84 PVDD147 pKa = 3.13 KK148 pKa = 10.28 PQDD151 pKa = 3.56 FGEE154 pKa = 4.33 VFNMFDD160 pKa = 4.29 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.38 NVHH172 pKa = 6.5 RR173 pKa = 11.84 DD174 pKa = 3.15 RR175 pKa = 11.84 YY176 pKa = 8.56 QVLRR180 pKa = 11.84 KK181 pKa = 8.38 WHH183 pKa = 5.41 STVTGGLYY191 pKa = 10.35 ASKK194 pKa = 10.33 EE195 pKa = 3.73 QALVKK200 pKa = 10.65 KK201 pKa = 9.55 FIRR204 pKa = 11.84 VNNHH208 pKa = 3.56 VVYY211 pKa = 10.34 NQQEE215 pKa = 3.83 AGKK218 pKa = 10.1 YY219 pKa = 8.23 EE220 pKa = 4.02 NHH222 pKa = 6.47 TEE224 pKa = 3.99 NALMLYY230 pKa = 7.52 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.67 FYY251 pKa = 11.26 DD252 pKa = 3.51 SVSNN256 pKa = 3.9
Molecular weight: 29.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.269
IPC2_protein 9.545
IPC_protein 9.78
Toseland 10.218
ProMoST 9.897
Dawson 10.409
Bjellqvist 10.087
Wikipedia 10.584
Rodwell 10.774
Grimsley 10.467
Solomon 10.438
Lehninger 10.394
Nozaki 10.233
DTASelect 10.072
Thurlkill 10.262
EMBOSS 10.613
Sillero 10.321
Patrickios 10.394
IPC_peptide 10.438
IPC2_peptide 8.946
IPC2.peptide.svr19 8.517
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1104
100
359
184.0
21.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.25 ± 0.757
2.174 ± 0.536
4.801 ± 0.333
4.982 ± 0.732
4.167 ± 0.347
5.072 ± 0.35
4.076 ± 0.675
4.71 ± 0.698
5.707 ± 0.747
6.884 ± 0.865
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.536 ± 0.574
5.616 ± 0.641
5.978 ± 0.58
4.801 ± 0.481
6.975 ± 1.307
8.243 ± 1.318
5.254 ± 0.545
6.612 ± 1.285
1.359 ± 0.234
3.804 ± 0.394
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here