Amino acid dipepetide frequency for Salmonella phage BSP161

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.414AlaAla: 9.414 ± 1.156
0.841AlaCys: 0.841 ± 0.209
5.716AlaAsp: 5.716 ± 1.037
6.556AlaGlu: 6.556 ± 0.764
3.362AlaPhe: 3.362 ± 0.485
8.237AlaGly: 8.237 ± 0.947
1.513AlaHis: 1.513 ± 0.265
5.127AlaIle: 5.127 ± 0.69
7.649AlaLys: 7.649 ± 0.875
6.808AlaLeu: 6.808 ± 1.004
2.774AlaMet: 2.774 ± 0.618
5.211AlaAsn: 5.211 ± 1.06
2.942AlaPro: 2.942 ± 0.551
3.867AlaGln: 3.867 ± 0.446
5.043AlaArg: 5.043 ± 0.613
4.791AlaSer: 4.791 ± 0.851
3.194AlaThr: 3.194 ± 0.517
5.464AlaVal: 5.464 ± 0.697
1.513AlaTrp: 1.513 ± 0.407
3.278AlaTyr: 3.278 ± 0.625
0.0AlaXaa: 0.0 ± 0.0
Cys
0.925CysAla: 0.925 ± 0.336
0.0CysCys: 0.0 ± 0.0
0.588CysAsp: 0.588 ± 0.314
0.672CysGlu: 0.672 ± 0.263
0.672CysPhe: 0.672 ± 0.255
0.672CysGly: 0.672 ± 0.214
0.168CysHis: 0.168 ± 0.116
0.252CysIle: 0.252 ± 0.162
0.252CysLys: 0.252 ± 0.135
1.009CysLeu: 1.009 ± 0.328
0.084CysMet: 0.084 ± 0.094
0.336CysAsn: 0.336 ± 0.163
0.336CysPro: 0.336 ± 0.207
0.42CysGln: 0.42 ± 0.188
0.672CysArg: 0.672 ± 0.287
0.672CysSer: 0.672 ± 0.235
0.336CysThr: 0.336 ± 0.199
0.504CysVal: 0.504 ± 0.216
0.252CysTrp: 0.252 ± 0.184
0.42CysTyr: 0.42 ± 0.232
0.0CysXaa: 0.0 ± 0.0
Asp
4.959AspAla: 4.959 ± 0.623
0.588AspCys: 0.588 ± 0.241
4.287AspAsp: 4.287 ± 0.653
3.951AspGlu: 3.951 ± 0.571
2.606AspPhe: 2.606 ± 0.467
6.556AspGly: 6.556 ± 0.803
1.177AspHis: 1.177 ± 0.289
3.278AspIle: 3.278 ± 0.382
3.782AspLys: 3.782 ± 0.668
3.194AspLeu: 3.194 ± 0.513
2.858AspMet: 2.858 ± 0.628
2.185AspAsn: 2.185 ± 0.345
3.278AspPro: 3.278 ± 0.558
2.017AspGln: 2.017 ± 0.408
3.362AspArg: 3.362 ± 0.893
3.782AspSer: 3.782 ± 0.57
3.53AspThr: 3.53 ± 0.468
3.951AspVal: 3.951 ± 0.508
0.756AspTrp: 0.756 ± 0.327
1.765AspTyr: 1.765 ± 0.325
0.0AspXaa: 0.0 ± 0.0
Glu
7.565GluAla: 7.565 ± 0.859
0.756GluCys: 0.756 ± 0.276
4.203GluAsp: 4.203 ± 0.565
5.464GluGlu: 5.464 ± 1.103
2.69GluPhe: 2.69 ± 0.437
4.539GluGly: 4.539 ± 0.616
1.597GluHis: 1.597 ± 0.419
2.858GluIle: 2.858 ± 0.444
3.026GluLys: 3.026 ± 0.646
6.808GluLeu: 6.808 ± 0.792
2.858GluMet: 2.858 ± 0.443
3.278GluAsn: 3.278 ± 0.628
2.017GluPro: 2.017 ± 0.399
3.782GluGln: 3.782 ± 0.546
4.707GluArg: 4.707 ± 0.759
3.53GluSer: 3.53 ± 0.6
3.782GluThr: 3.782 ± 0.475
4.539GluVal: 4.539 ± 0.627
1.261GluTrp: 1.261 ± 0.329
3.11GluTyr: 3.11 ± 0.457
0.0GluXaa: 0.0 ± 0.0
Phe
2.942PheAla: 2.942 ± 0.648
0.504PheCys: 0.504 ± 0.211
2.269PheAsp: 2.269 ± 0.551
2.017PheGlu: 2.017 ± 0.365
0.925PhePhe: 0.925 ± 0.277
3.194PheGly: 3.194 ± 0.433
0.588PheHis: 0.588 ± 0.239
1.681PheIle: 1.681 ± 0.347
2.522PheLys: 2.522 ± 0.515
3.11PheLeu: 3.11 ± 0.448
1.177PheMet: 1.177 ± 0.282
1.681PheAsn: 1.681 ± 0.429
1.765PhePro: 1.765 ± 0.409
1.429PheGln: 1.429 ± 0.347
2.101PheArg: 2.101 ± 0.367
1.849PheSer: 1.849 ± 0.442
3.194PheThr: 3.194 ± 0.435
2.185PheVal: 2.185 ± 0.406
0.252PheTrp: 0.252 ± 0.148
1.177PheTyr: 1.177 ± 0.269
0.0PheXaa: 0.0 ± 0.0
Gly
7.061GlyAla: 7.061 ± 0.964
0.504GlyCys: 0.504 ± 0.248
5.8GlyAsp: 5.8 ± 0.746
5.38GlyGlu: 5.38 ± 0.716
2.858GlyPhe: 2.858 ± 0.581
5.8GlyGly: 5.8 ± 0.767
1.093GlyHis: 1.093 ± 0.345
3.867GlyIle: 3.867 ± 0.68
7.061GlyLys: 7.061 ± 0.912
6.892GlyLeu: 6.892 ± 0.958
2.606GlyMet: 2.606 ± 0.38
3.614GlyAsn: 3.614 ± 0.635
0.42GlyPro: 0.42 ± 0.154
3.11GlyGln: 3.11 ± 0.476
4.539GlyArg: 4.539 ± 0.726
4.959GlySer: 4.959 ± 0.611
3.614GlyThr: 3.614 ± 0.387
4.791GlyVal: 4.791 ± 0.756
2.101GlyTrp: 2.101 ± 0.477
2.858GlyTyr: 2.858 ± 0.546
0.0GlyXaa: 0.0 ± 0.0
His
1.765HisAla: 1.765 ± 0.441
0.252HisCys: 0.252 ± 0.13
1.093HisAsp: 1.093 ± 0.291
1.261HisGlu: 1.261 ± 0.343
1.093HisPhe: 1.093 ± 0.272
1.345HisGly: 1.345 ± 0.325
0.336HisHis: 0.336 ± 0.175
1.261HisIle: 1.261 ± 0.362
1.093HisLys: 1.093 ± 0.306
1.513HisLeu: 1.513 ± 0.409
0.504HisMet: 0.504 ± 0.151
0.925HisAsn: 0.925 ± 0.327
0.252HisPro: 0.252 ± 0.139
0.252HisGln: 0.252 ± 0.135
0.672HisArg: 0.672 ± 0.214
1.009HisSer: 1.009 ± 0.27
0.925HisThr: 0.925 ± 0.29
1.681HisVal: 1.681 ± 0.307
0.336HisTrp: 0.336 ± 0.17
0.336HisTyr: 0.336 ± 0.145
0.0HisXaa: 0.0 ± 0.0
Ile
4.287IleAla: 4.287 ± 0.527
0.588IleCys: 0.588 ± 0.248
4.119IleAsp: 4.119 ± 0.658
3.362IleGlu: 3.362 ± 0.482
1.093IlePhe: 1.093 ± 0.258
3.951IleGly: 3.951 ± 0.514
1.177IleHis: 1.177 ± 0.358
2.522IleIle: 2.522 ± 0.508
3.782IleLys: 3.782 ± 0.612
3.362IleLeu: 3.362 ± 0.47
0.925IleMet: 0.925 ± 0.3
2.354IleAsn: 2.354 ± 0.553
2.354IlePro: 2.354 ± 0.475
1.597IleGln: 1.597 ± 0.361
3.362IleArg: 3.362 ± 0.533
2.858IleSer: 2.858 ± 0.648
2.606IleThr: 2.606 ± 0.509
2.942IleVal: 2.942 ± 0.492
0.672IleTrp: 0.672 ± 0.186
1.765IleTyr: 1.765 ± 0.267
0.0IleXaa: 0.0 ± 0.0
Lys
8.069LysAla: 8.069 ± 0.852
0.588LysCys: 0.588 ± 0.264
4.119LysAsp: 4.119 ± 0.531
5.716LysGlu: 5.716 ± 0.61
2.101LysPhe: 2.101 ± 0.391
5.295LysGly: 5.295 ± 0.718
1.681LysHis: 1.681 ± 0.458
2.017LysIle: 2.017 ± 0.39
5.632LysLys: 5.632 ± 0.907
5.464LysLeu: 5.464 ± 0.785
2.438LysMet: 2.438 ± 0.376
2.101LysAsn: 2.101 ± 0.357
2.858LysPro: 2.858 ± 0.652
2.438LysGln: 2.438 ± 0.505
3.698LysArg: 3.698 ± 0.719
4.035LysSer: 4.035 ± 0.711
3.53LysThr: 3.53 ± 0.513
5.548LysVal: 5.548 ± 0.636
0.925LysTrp: 0.925 ± 0.272
2.269LysTyr: 2.269 ± 0.413
0.0LysXaa: 0.0 ± 0.0
Leu
8.994LeuAla: 8.994 ± 0.828
0.252LeuCys: 0.252 ± 0.16
3.951LeuAsp: 3.951 ± 0.505
6.052LeuGlu: 6.052 ± 1.107
2.354LeuPhe: 2.354 ± 0.459
3.782LeuGly: 3.782 ± 0.581
1.093LeuHis: 1.093 ± 0.262
4.119LeuIle: 4.119 ± 0.537
6.977LeuLys: 6.977 ± 0.892
4.707LeuLeu: 4.707 ± 0.881
2.606LeuMet: 2.606 ± 0.51
4.287LeuAsn: 4.287 ± 0.69
2.858LeuPro: 2.858 ± 0.53
3.362LeuGln: 3.362 ± 0.539
5.464LeuArg: 5.464 ± 0.564
4.203LeuSer: 4.203 ± 0.529
4.707LeuThr: 4.707 ± 0.814
5.464LeuVal: 5.464 ± 0.605
1.261LeuTrp: 1.261 ± 0.398
2.354LeuTyr: 2.354 ± 0.439
0.0LeuXaa: 0.0 ± 0.0
Met
2.774MetAla: 2.774 ± 0.482
0.252MetCys: 0.252 ± 0.138
1.597MetAsp: 1.597 ± 0.441
2.185MetGlu: 2.185 ± 0.469
1.429MetPhe: 1.429 ± 0.301
2.69MetGly: 2.69 ± 0.523
0.336MetHis: 0.336 ± 0.213
1.429MetIle: 1.429 ± 0.316
1.765MetLys: 1.765 ± 0.542
3.278MetLeu: 3.278 ± 0.476
0.672MetMet: 0.672 ± 0.215
1.597MetAsn: 1.597 ± 0.44
1.429MetPro: 1.429 ± 0.368
1.429MetGln: 1.429 ± 0.413
1.093MetArg: 1.093 ± 0.303
2.017MetSer: 2.017 ± 0.326
1.849MetThr: 1.849 ± 0.371
2.269MetVal: 2.269 ± 0.478
0.168MetTrp: 0.168 ± 0.133
0.841MetTyr: 0.841 ± 0.225
0.0MetXaa: 0.0 ± 0.0
Asn
3.446AsnAla: 3.446 ± 0.555
0.588AsnCys: 0.588 ± 0.204
3.11AsnAsp: 3.11 ± 0.682
3.194AsnGlu: 3.194 ± 0.551
1.933AsnPhe: 1.933 ± 0.299
5.295AsnGly: 5.295 ± 1.116
1.009AsnHis: 1.009 ± 0.307
2.522AsnIle: 2.522 ± 0.438
3.362AsnLys: 3.362 ± 0.56
3.446AsnLeu: 3.446 ± 0.469
1.513AsnMet: 1.513 ± 0.42
1.513AsnAsn: 1.513 ± 0.385
2.101AsnPro: 2.101 ± 0.337
1.429AsnGln: 1.429 ± 0.299
2.69AsnArg: 2.69 ± 0.849
2.69AsnSer: 2.69 ± 0.674
2.606AsnThr: 2.606 ± 0.651
2.69AsnVal: 2.69 ± 0.498
0.504AsnTrp: 0.504 ± 0.202
2.017AsnTyr: 2.017 ± 0.436
0.0AsnXaa: 0.0 ± 0.0
Pro
2.69ProAla: 2.69 ± 0.462
0.336ProCys: 0.336 ± 0.183
2.438ProAsp: 2.438 ± 0.487
3.698ProGlu: 3.698 ± 0.686
1.345ProPhe: 1.345 ± 0.352
1.429ProGly: 1.429 ± 0.279
0.672ProHis: 0.672 ± 0.236
1.513ProIle: 1.513 ± 0.296
2.69ProLys: 2.69 ± 0.513
2.269ProLeu: 2.269 ± 0.494
0.925ProMet: 0.925 ± 0.401
2.438ProAsn: 2.438 ± 0.442
0.756ProPro: 0.756 ± 0.225
0.756ProGln: 0.756 ± 0.247
1.429ProArg: 1.429 ± 0.29
2.101ProSer: 2.101 ± 0.427
2.354ProThr: 2.354 ± 0.44
3.11ProVal: 3.11 ± 0.519
0.504ProTrp: 0.504 ± 0.18
1.261ProTyr: 1.261 ± 0.341
0.0ProXaa: 0.0 ± 0.0
Gln
4.875GlnAla: 4.875 ± 0.561
0.084GlnCys: 0.084 ± 0.091
1.933GlnAsp: 1.933 ± 0.346
2.522GlnGlu: 2.522 ± 0.48
2.269GlnPhe: 2.269 ± 0.334
2.858GlnGly: 2.858 ± 0.615
0.588GlnHis: 0.588 ± 0.226
2.017GlnIle: 2.017 ± 0.463
1.513GlnLys: 1.513 ± 0.459
3.782GlnLeu: 3.782 ± 0.53
1.177GlnMet: 1.177 ± 0.251
1.177GlnAsn: 1.177 ± 0.284
1.597GlnPro: 1.597 ± 0.343
1.849GlnGln: 1.849 ± 0.401
1.429GlnArg: 1.429 ± 0.288
1.849GlnSer: 1.849 ± 0.393
1.849GlnThr: 1.849 ± 0.447
2.269GlnVal: 2.269 ± 0.372
0.588GlnTrp: 0.588 ± 0.181
1.345GlnTyr: 1.345 ± 0.293
0.0GlnXaa: 0.0 ± 0.0
Arg
4.287ArgAla: 4.287 ± 0.852
0.841ArgCys: 0.841 ± 0.256
3.362ArgAsp: 3.362 ± 0.533
4.035ArgGlu: 4.035 ± 0.758
1.933ArgPhe: 1.933 ± 0.414
4.035ArgGly: 4.035 ± 0.572
0.925ArgHis: 0.925 ± 0.248
2.354ArgIle: 2.354 ± 0.534
4.287ArgLys: 4.287 ± 0.739
4.791ArgLeu: 4.791 ± 0.729
1.597ArgMet: 1.597 ± 0.366
3.614ArgAsn: 3.614 ± 0.712
1.765ArgPro: 1.765 ± 0.301
1.597ArgGln: 1.597 ± 0.362
2.017ArgArg: 2.017 ± 0.328
3.951ArgSer: 3.951 ± 0.685
3.026ArgThr: 3.026 ± 0.385
3.867ArgVal: 3.867 ± 0.608
0.756ArgTrp: 0.756 ± 0.315
1.345ArgTyr: 1.345 ± 0.226
0.0ArgXaa: 0.0 ± 0.0
Ser
4.707SerAla: 4.707 ± 0.744
0.42SerCys: 0.42 ± 0.287
3.867SerAsp: 3.867 ± 0.539
3.782SerGlu: 3.782 ± 0.618
2.354SerPhe: 2.354 ± 0.465
5.38SerGly: 5.38 ± 0.639
1.345SerHis: 1.345 ± 0.49
2.942SerIle: 2.942 ± 0.421
3.53SerLys: 3.53 ± 0.502
3.698SerLeu: 3.698 ± 0.511
2.017SerMet: 2.017 ± 0.463
2.858SerAsn: 2.858 ± 0.603
2.017SerPro: 2.017 ± 0.338
2.354SerGln: 2.354 ± 0.495
3.11SerArg: 3.11 ± 0.471
2.522SerSer: 2.522 ± 0.494
3.026SerThr: 3.026 ± 0.434
3.951SerVal: 3.951 ± 0.6
0.504SerTrp: 0.504 ± 0.194
2.101SerTyr: 2.101 ± 0.63
0.0SerXaa: 0.0 ± 0.0
Thr
4.455ThrAla: 4.455 ± 0.812
0.588ThrCys: 0.588 ± 0.248
3.194ThrAsp: 3.194 ± 0.426
4.791ThrGlu: 4.791 ± 0.467
1.849ThrPhe: 1.849 ± 0.35
5.211ThrGly: 5.211 ± 0.738
1.009ThrHis: 1.009 ± 0.341
3.698ThrIle: 3.698 ± 0.669
3.53ThrLys: 3.53 ± 0.541
5.211ThrLeu: 5.211 ± 0.846
1.093ThrMet: 1.093 ± 0.311
1.765ThrAsn: 1.765 ± 0.4
2.354ThrPro: 2.354 ± 0.375
1.597ThrGln: 1.597 ± 0.374
2.606ThrArg: 2.606 ± 0.387
3.278ThrSer: 3.278 ± 0.545
3.026ThrThr: 3.026 ± 0.546
3.614ThrVal: 3.614 ± 0.547
0.588ThrTrp: 0.588 ± 0.199
1.261ThrTyr: 1.261 ± 0.317
0.0ThrXaa: 0.0 ± 0.0
Val
6.388ValAla: 6.388 ± 0.82
0.42ValCys: 0.42 ± 0.197
3.362ValAsp: 3.362 ± 0.38
4.791ValGlu: 4.791 ± 0.719
2.606ValPhe: 2.606 ± 0.565
4.623ValGly: 4.623 ± 0.589
1.009ValHis: 1.009 ± 0.258
3.53ValIle: 3.53 ± 0.581
4.539ValLys: 4.539 ± 0.599
5.548ValLeu: 5.548 ± 0.751
1.849ValMet: 1.849 ± 0.455
3.698ValAsn: 3.698 ± 0.814
2.354ValPro: 2.354 ± 0.353
2.438ValGln: 2.438 ± 0.557
4.203ValArg: 4.203 ± 0.778
4.035ValSer: 4.035 ± 0.552
4.287ValThr: 4.287 ± 0.553
4.119ValVal: 4.119 ± 0.797
1.093ValTrp: 1.093 ± 0.453
1.933ValTyr: 1.933 ± 0.534
0.0ValXaa: 0.0 ± 0.0
Trp
0.504TrpAla: 0.504 ± 0.204
0.42TrpCys: 0.42 ± 0.233
0.336TrpAsp: 0.336 ± 0.165
1.009TrpGlu: 1.009 ± 0.241
0.42TrpPhe: 0.42 ± 0.203
1.009TrpGly: 1.009 ± 0.258
0.252TrpHis: 0.252 ± 0.149
0.841TrpIle: 0.841 ± 0.284
1.681TrpLys: 1.681 ± 0.352
1.597TrpLeu: 1.597 ± 0.386
0.504TrpMet: 0.504 ± 0.173
1.009TrpAsn: 1.009 ± 0.329
0.252TrpPro: 0.252 ± 0.132
0.588TrpGln: 0.588 ± 0.209
0.588TrpArg: 0.588 ± 0.234
1.009TrpSer: 1.009 ± 0.33
1.093TrpThr: 1.093 ± 0.322
1.261TrpVal: 1.261 ± 0.367
0.252TrpTrp: 0.252 ± 0.132
0.252TrpTyr: 0.252 ± 0.153
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.194TyrAla: 3.194 ± 0.399
0.42TyrCys: 0.42 ± 0.196
2.522TyrAsp: 2.522 ± 0.413
1.933TyrGlu: 1.933 ± 0.452
0.672TyrPhe: 0.672 ± 0.231
3.11TyrGly: 3.11 ± 0.42
0.336TyrHis: 0.336 ± 0.16
1.849TyrIle: 1.849 ± 0.455
1.849TyrLys: 1.849 ± 0.404
2.354TyrLeu: 2.354 ± 0.421
0.841TyrMet: 0.841 ± 0.23
2.017TyrAsn: 2.017 ± 0.377
1.009TyrPro: 1.009 ± 0.317
1.261TyrGln: 1.261 ± 0.384
1.597TyrArg: 1.597 ± 0.316
1.345TyrSer: 1.345 ± 0.295
2.185TyrThr: 2.185 ± 0.436
2.606TyrVal: 2.606 ± 0.439
0.588TyrTrp: 0.588 ± 0.193
0.756TyrTyr: 0.756 ± 0.218
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (11898 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski