Streptococcus phage Javan583
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AJM9|A0A4D6AJM9_9CAUD Uncharacterized protein OS=Streptococcus phage Javan583 OX=2548273 GN=Javan583_0044 PE=4 SV=1
MM1 pKa = 7.17 QFDD4 pKa = 3.89 EE5 pKa = 5.04 VIEE8 pKa = 4.64 FNDD11 pKa = 3.32 EE12 pKa = 4.16 LYY14 pKa = 10.82 QKK16 pKa = 10.78 NIADD20 pKa = 3.7 HH21 pKa = 6.52 TFPEE25 pKa = 5.29 IDD27 pKa = 4.23 DD28 pKa = 4.62 LDD30 pKa = 4.41 GKK32 pKa = 11.21 GGTNEE37 pKa = 3.71
Molecular weight: 4.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.253
IPC2_protein 3.808
IPC_protein 3.681
Toseland 3.49
ProMoST 3.821
Dawson 3.681
Bjellqvist 3.948
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.414
Solomon 3.643
Lehninger 3.605
Nozaki 3.834
DTASelect 3.999
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.808
Patrickios 3.503
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.785
Protein with the highest isoelectric point:
>tr|A0A4D6AJX1|A0A4D6AJX1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan583 OX=2548273 GN=Javan583_0022 PE=4 SV=1
MM1 pKa = 7.71 AKK3 pKa = 10.24 NGAKK7 pKa = 10.32 GGGRR11 pKa = 11.84 HH12 pKa = 5.47 GAVKK16 pKa = 10.34 GRR18 pKa = 11.84 SQSYY22 pKa = 9.31 NPKK25 pKa = 8.86 TGLYY29 pKa = 7.82 TKK31 pKa = 10.46 RR32 pKa = 11.84 NTSTGKK38 pKa = 10.51 FMDD41 pKa = 3.49 VKK43 pKa = 8.92 TTGGKK48 pKa = 9.72 FKK50 pKa = 11.02 GVTSEE55 pKa = 4.15 KK56 pKa = 10.95
Molecular weight: 5.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.214
IPC2_protein 10.072
IPC_protein 10.335
Toseland 11.008
ProMoST 10.555
Dawson 11.067
Bjellqvist 10.672
Wikipedia 11.184
Rodwell 11.711
Grimsley 11.096
Solomon 11.125
Lehninger 11.111
Nozaki 10.965
DTASelect 10.672
Thurlkill 10.965
EMBOSS 11.374
Sillero 10.979
Patrickios 11.462
IPC_peptide 11.14
IPC2_peptide 9.151
IPC2.peptide.svr19 8.467
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
0
57
9404
37
1387
165.0
18.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.401 ± 0.556
0.5 ± 0.108
5.859 ± 0.266
7.21 ± 0.543
3.86 ± 0.216
6.933 ± 0.577
1.404 ± 0.173
6.699 ± 0.301
7.614 ± 0.487
8.55 ± 0.425
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.69 ± 0.242
5.296 ± 0.346
2.882 ± 0.289
4.381 ± 0.309
4.849 ± 0.368
6.114 ± 0.475
6.285 ± 0.406
6.402 ± 0.303
1.223 ± 0.121
3.849 ± 0.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here