Streptomyces phage Geostin

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Beephvirinae; Flowerpowervirus; unclassified Flowerpowervirus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8RNQ1|A0A5B8RNQ1_9CAUD Holin OS=Streptomyces phage Geostin OX=2596979 GN=44 PE=4 SV=1
MM1 pKa = 7.2STTISLFVQDD11 pKa = 3.75QDD13 pKa = 4.49GEE15 pKa = 4.36HH16 pKa = 6.26TEE18 pKa = 4.0IDD20 pKa = 4.6LDD22 pKa = 4.29DD23 pKa = 3.57VWIQSDD29 pKa = 4.23VIDD32 pKa = 3.96EE33 pKa = 4.37EE34 pKa = 4.78SFHH37 pKa = 6.34GALVAKK43 pKa = 9.91IATWALEE50 pKa = 4.16RR51 pKa = 11.84GSSFDD56 pKa = 5.03DD57 pKa = 3.35ISGLTVDD64 pKa = 5.04DD65 pKa = 5.74DD66 pKa = 4.17EE67 pKa = 4.95GQEE70 pKa = 4.3LEE72 pKa = 4.03IHH74 pKa = 6.31NEE76 pKa = 3.81TGEE79 pKa = 4.13NRR81 pKa = 11.84HH82 pKa = 4.77SWILEE87 pKa = 4.01VVQLYY92 pKa = 8.14MWLNCDD98 pKa = 2.66KK99 pKa = 10.67HH100 pKa = 7.3YY101 pKa = 11.44CEE103 pKa = 5.9DD104 pKa = 3.6EE105 pKa = 5.22AILARR110 pKa = 11.84VDD112 pKa = 3.95DD113 pKa = 5.27IGWKK117 pKa = 9.56WFDD120 pKa = 3.85FDD122 pKa = 6.0SDD124 pKa = 3.8LQEE127 pKa = 5.76AEE129 pKa = 4.62DD130 pKa = 4.33MYY132 pKa = 11.46SQEE135 pKa = 3.9FDD137 pKa = 3.03GDD139 pKa = 3.67YY140 pKa = 11.33AEE142 pKa = 4.46YY143 pKa = 10.62ARR145 pKa = 11.84EE146 pKa = 3.92YY147 pKa = 10.32MDD149 pKa = 3.9GCGEE153 pKa = 4.27SLSEE157 pKa = 3.78WAEE160 pKa = 3.91KK161 pKa = 10.58YY162 pKa = 10.28FDD164 pKa = 3.75YY165 pKa = 11.33EE166 pKa = 4.22EE167 pKa = 4.52YY168 pKa = 11.31GEE170 pKa = 4.8DD171 pKa = 4.73LVDD174 pKa = 4.55GYY176 pKa = 11.61DD177 pKa = 3.31RR178 pKa = 11.84VSWGSRR184 pKa = 11.84EE185 pKa = 3.92FLFSQQ190 pKa = 4.23

Molecular weight: 22.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8RU78|A0A5B8RU78_9CAUD Uncharacterized protein OS=Streptomyces phage Geostin OX=2596979 GN=80 PE=4 SV=1
MM1 pKa = 7.48IAFDD5 pKa = 4.23EE6 pKa = 4.7TFMSAGDD13 pKa = 3.67PMVTTRR19 pKa = 11.84IPGFGSGNRR28 pKa = 11.84TPQTSRR34 pKa = 11.84VHH36 pKa = 5.36VSRR39 pKa = 11.84AKK41 pKa = 10.33GVKK44 pKa = 8.97QGKK47 pKa = 8.36PVTKK51 pKa = 10.12GLARR55 pKa = 11.84SAVAVVRR62 pKa = 11.84VNPRR66 pKa = 11.84KK67 pKa = 9.95FEE69 pKa = 3.79IRR71 pKa = 11.84VKK73 pKa = 10.7GSRR76 pKa = 11.84ALLGFVTKK84 pKa = 10.75VKK86 pKa = 10.57GAGGKK91 pKa = 9.93AYY93 pKa = 9.82AYY95 pKa = 10.29QCLLPNTDD103 pKa = 3.44AKK105 pKa = 9.4VHH107 pKa = 6.34KK108 pKa = 10.57GFATQKK114 pKa = 8.84EE115 pKa = 4.44AVARR119 pKa = 11.84MLEE122 pKa = 4.12KK123 pKa = 10.82CC124 pKa = 4.05

Molecular weight: 13.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13348

40

1635

202.2

22.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.987 ± 0.379

0.682 ± 0.146

6.54 ± 0.293

5.604 ± 0.332

3.132 ± 0.157

8.458 ± 0.335

1.573 ± 0.153

4.195 ± 0.219

5.746 ± 0.369

7.424 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.21

4.083 ± 0.243

4.443 ± 0.257

4.458 ± 0.336

4.945 ± 0.274

6.435 ± 0.305

7.012 ± 0.473

7.012 ± 0.379

1.738 ± 0.125

3.813 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski