Synechococcus phage S-ShM2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ahtivirus; Synechococcus virus SShM2

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 230 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3SJT4|E3SJT4_9CAUD Uncharacterized protein OS=Synechococcus phage S-ShM2 OX=445683 GN=SShM2_033 PE=4 SV=1
MM1 pKa = 7.28ATQTGKK7 pKa = 10.35LASAKK12 pKa = 9.73PAATTWTALYY22 pKa = 8.84RR23 pKa = 11.84APIDD27 pKa = 3.72SSASGVLNMVSDD39 pKa = 4.32GTAANVRR46 pKa = 11.84VGVKK50 pKa = 10.06KK51 pKa = 10.81YY52 pKa = 10.91DD53 pKa = 3.17IAATLDD59 pKa = 3.14AATYY63 pKa = 9.62LLHH66 pKa = 6.93PGDD69 pKa = 4.27VITNKK74 pKa = 9.28TLTFDD79 pKa = 3.32TSIPIITDD87 pKa = 3.23QQDD90 pKa = 3.19TFTPGQLITTNDD102 pKa = 3.4GEE104 pKa = 4.86TTFKK108 pKa = 10.34WEE110 pKa = 4.1SYY112 pKa = 9.23YY113 pKa = 10.96VPPSTDD119 pKa = 3.27FYY121 pKa = 9.41VQKK124 pKa = 10.57IGILSYY130 pKa = 10.75SIEE133 pKa = 4.11NQTGSFTVGEE143 pKa = 4.51TVTGGTGSGTAVIYY157 pKa = 10.26DD158 pKa = 4.18VIDD161 pKa = 4.14GSLGGSTLYY170 pKa = 10.65LGPVTGTTFVEE181 pKa = 5.09GEE183 pKa = 4.35TLTGGTSSATGDD195 pKa = 3.09ISAGGVGVSRR205 pKa = 11.84DD206 pKa = 3.44EE207 pKa = 5.8LVFSDD212 pKa = 3.63NGSGGTYY219 pKa = 8.96SLRR222 pKa = 11.84RR223 pKa = 11.84STTLTLFLDD232 pKa = 3.69RR233 pKa = 11.84TYY235 pKa = 11.54KK236 pKa = 10.63FFVEE240 pKa = 4.54DD241 pKa = 3.33SSMSGVGFGLSTTINGTFGIDD262 pKa = 3.33QTAGTSDD269 pKa = 4.32DD270 pKa = 3.48GTEE273 pKa = 4.02YY274 pKa = 8.27TTGKK278 pKa = 8.52TSSGTAGSSGAYY290 pKa = 8.54VQYY293 pKa = 11.45DD294 pKa = 3.29MSANGGGDD302 pKa = 2.92ATYY305 pKa = 10.87YY306 pKa = 11.12YY307 pKa = 10.88FDD309 pKa = 4.45TLDD312 pKa = 3.65GTLGGGDD319 pKa = 3.33QSLQLSVDD327 pKa = 3.37YY328 pKa = 10.88SYY330 pKa = 10.68DD331 pKa = 3.24TIYY334 pKa = 10.3IYY336 pKa = 10.72DD337 pKa = 3.96LQGTLTNASDD347 pKa = 5.4AITLGQTTFTLTSNAGSKK365 pKa = 8.42WGYY368 pKa = 7.37VQEE371 pKa = 4.29IDD373 pKa = 3.59GTSVNIVTGLGSADD387 pKa = 3.35FAGADD392 pKa = 3.44TFYY395 pKa = 10.92DD396 pKa = 3.75VPKK399 pKa = 10.21IGTASRR405 pKa = 11.84SLATISSIVTAANAIDD421 pKa = 4.01SVDD424 pKa = 3.25TIMFDD429 pKa = 3.45NSVDD433 pKa = 3.25AAEE436 pKa = 4.64RR437 pKa = 11.84LTSLVIGPGQVLMVYY452 pKa = 10.24AATQNISFDD461 pKa = 3.46YY462 pKa = 11.21SGFQDD467 pKa = 3.21SSSDD471 pKa = 3.41FTVRR475 pKa = 11.84SFDD478 pKa = 3.66VNASIAADD486 pKa = 3.87GASGGGG492 pKa = 3.32

Molecular weight: 50.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3SJQ9|E3SJQ9_9CAUD Head completion protein OS=Synechococcus phage S-ShM2 OX=445683 GN=gp4 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.37KK3 pKa = 10.28VLLLIAGAAFFAAPVQAHH21 pKa = 5.31QAHH24 pKa = 6.88KK25 pKa = 10.27HH26 pKa = 5.54RR27 pKa = 11.84NHH29 pKa = 5.97HH30 pKa = 4.92HH31 pKa = 6.77HH32 pKa = 6.33YY33 pKa = 10.72NNVEE37 pKa = 4.07RR38 pKa = 11.84YY39 pKa = 6.93FTCHH43 pKa = 5.63DD44 pKa = 3.44HH45 pKa = 6.24LRR47 pKa = 11.84RR48 pKa = 11.84NIRR51 pKa = 11.84HH52 pKa = 5.71CHH54 pKa = 4.07GHH56 pKa = 5.26TNWGHH61 pKa = 4.9GRR63 pKa = 11.84QLRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84DD69 pKa = 3.47YY70 pKa = 9.66FAPSIIFDD78 pKa = 3.81FF79 pKa = 4.75

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

230

0

230

58486

24

6198

254.3

28.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.841 ± 0.214

0.983 ± 0.089

6.737 ± 0.145

5.995 ± 0.268

4.283 ± 0.091

7.904 ± 0.346

1.477 ± 0.105

6.319 ± 0.202

5.658 ± 0.406

7.099 ± 0.148

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.193

5.701 ± 0.175

4.045 ± 0.149

3.85 ± 0.096

4.275 ± 0.169

7.185 ± 0.165

7.34 ± 0.397

6.615 ± 0.176

1.185 ± 0.09

4.259 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski