Alligator mississippiensis (American alligator)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Crocodylia; Alligatoridae;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31974 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A151MTB3|A0A151MTB3_ALLMI Receptor protein serine/threonine kinase OS=Alligator mississippiensis OX=8496 GN=Y1Q_0005300 PE=3 SV=1
MM1 pKa = 7.38VAPQPAVEE9 pKa = 4.68DD10 pKa = 3.49VGEE13 pKa = 4.19AALVVNDD20 pKa = 4.17FLQDD24 pKa = 3.64DD25 pKa = 4.68GEE27 pKa = 4.6VAFAVYY33 pKa = 8.23EE34 pKa = 4.18HH35 pKa = 7.35PGALEE40 pKa = 3.81WTEE43 pKa = 4.6DD44 pKa = 3.54GDD46 pKa = 3.61VGAIHH51 pKa = 7.19GPPASGKK58 pKa = 10.22AYY60 pKa = 9.95VVEE63 pKa = 4.83AGDD66 pKa = 3.49YY67 pKa = 8.56FLWVGEE73 pKa = 4.27VYY75 pKa = 10.47KK76 pKa = 11.16VVGQDD81 pKa = 3.6ILDD84 pKa = 4.33DD85 pKa = 3.9QADD88 pKa = 4.2LLDD91 pKa = 4.15SLSGHH96 pKa = 6.59GFTHH100 pKa = 5.99TNWLAMM106 pKa = 4.31

Molecular weight: 11.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A151PG72|A0A151PG72_ALLMI IRG-type G domain-containing protein OS=Alligator mississippiensis OX=8496 GN=Y1Q_0020326 PE=3 SV=1
MM1 pKa = 7.35SKK3 pKa = 9.86WSRR6 pKa = 11.84LSSQEE11 pKa = 3.4HH12 pKa = 6.48SRR14 pKa = 11.84TQRR17 pKa = 11.84RR18 pKa = 11.84SGPGGAAAGQQPSPPASGGARR39 pKa = 11.84ALRR42 pKa = 11.84PPPSLPAAAGPGPQHH57 pKa = 7.17RR58 pKa = 11.84GFLLARR64 pKa = 11.84PATASPQRR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84EE77 pKa = 3.89RR78 pKa = 11.84RR79 pKa = 11.84WLLL82 pKa = 3.42

Molecular weight: 8.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24656

7318

31974

16476323

66

16830

515.3

57.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.764 ± 0.014

2.296 ± 0.015

4.95 ± 0.01

7.299 ± 0.021

3.545 ± 0.009

6.184 ± 0.018

2.57 ± 0.007

4.655 ± 0.012

6.12 ± 0.018

9.717 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.29 ± 0.007

3.843 ± 0.009

5.737 ± 0.021

4.926 ± 0.014

5.395 ± 0.012

8.243 ± 0.017

5.453 ± 0.011

6.003 ± 0.012

1.238 ± 0.005

2.742 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski