Hungateiclostridiaceae bacterium KB18

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; unclassified Oscillospiraceae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3593 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C7GNG5|A0A1C7GNG5_9FIRM ABC transporter permease OS=Hungateiclostridiaceae bacterium KB18 OX=1834198 GN=A4V00_03700 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.47KK3 pKa = 10.39LLALVLALCLVLGLGGCRR21 pKa = 11.84FINKK25 pKa = 6.24TTAPVEE31 pKa = 4.23FTSADD36 pKa = 3.29SGTLYY41 pKa = 8.68WHH43 pKa = 6.22SMEE46 pKa = 4.74LAGLIDD52 pKa = 5.69DD53 pKa = 4.07MLKK56 pKa = 8.67TPEE59 pKa = 4.13YY60 pKa = 9.52FEE62 pKa = 5.74LMGGSDD68 pKa = 3.62TVSEE72 pKa = 4.42VIIPLMRR79 pKa = 11.84SDD81 pKa = 3.69LSEE84 pKa = 4.4PEE86 pKa = 3.76SAYY89 pKa = 11.01VVTLPEE95 pKa = 4.64DD96 pKa = 3.82ALPTLMSAAEE106 pKa = 3.89VDD108 pKa = 3.71LDD110 pKa = 3.94GFSDD114 pKa = 4.09GLRR117 pKa = 11.84EE118 pKa = 3.99FVEE121 pKa = 4.15RR122 pKa = 11.84RR123 pKa = 11.84MASSVPTILNSRR135 pKa = 11.84QGAEE139 pKa = 3.89TLAASSILTVSGSWAGEE156 pKa = 3.96VLDD159 pKa = 4.38EE160 pKa = 4.26SCYY163 pKa = 10.49VLLMYY168 pKa = 8.04PYY170 pKa = 9.59CCPVMASFTGSNGVVTGTATMLLGEE195 pKa = 4.46PLEE198 pKa = 4.5EE199 pKa = 5.22DD200 pKa = 3.2SLDD203 pKa = 3.6AVEE206 pKa = 5.18EE207 pKa = 4.12LFGYY211 pKa = 10.9LEE213 pKa = 4.13MEE215 pKa = 4.31GPEE218 pKa = 4.08IEE220 pKa = 5.25EE221 pKa = 3.86IDD223 pKa = 3.46IDD225 pKa = 4.41ALII228 pKa = 4.48

Molecular weight: 24.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0QDZ3|A0A1V0QDZ3_9FIRM Uncharacterized protein OS=Hungateiclostridiaceae bacterium KB18 OX=1834198 GN=A4V00_19230 PE=4 SV=1
MM1 pKa = 7.53HH2 pKa = 7.92PLHH5 pKa = 6.58LQLPPLEE12 pKa = 4.47EE13 pKa = 3.99LHH15 pKa = 6.74LLRR18 pKa = 11.84PSGAGVRR25 pKa = 11.84RR26 pKa = 11.84QGDD29 pKa = 3.98LRR31 pKa = 11.84FMLCEE36 pKa = 3.79ASINQQSLTQFMDD49 pKa = 3.29RR50 pKa = 11.84LVRR53 pKa = 11.84TSLRR57 pKa = 11.84KK58 pKa = 9.95VFLILDD64 pKa = 3.83NLKK67 pKa = 10.19GHH69 pKa = 6.77HH70 pKa = 6.26GKK72 pKa = 9.97KK73 pKa = 9.9AAARR77 pKa = 11.84LDD79 pKa = 3.42RR80 pKa = 11.84HH81 pKa = 5.83RR82 pKa = 11.84KK83 pKa = 9.41KK84 pKa = 10.53IEE86 pKa = 3.89LFLLAAYY93 pKa = 8.74LRR95 pKa = 11.84PNVISAIKK103 pKa = 9.33PPPSCEE109 pKa = 4.07PYY111 pKa = 9.89NLLPIRR117 pKa = 11.84SPLSFCMIRR126 pKa = 11.84FPVFLFGGNTPPEE139 pKa = 4.73DD140 pKa = 3.27GFAPQGRR147 pKa = 11.84GCVYY151 pKa = 10.72LSGRR155 pKa = 11.84PGKK158 pKa = 10.58SGGRR162 pKa = 11.84VLTCHH167 pKa = 6.15CQGPLRR173 pKa = 11.84SLGGRR178 pKa = 11.84RR179 pKa = 11.84LLSS182 pKa = 3.49

Molecular weight: 20.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3593

0

3593

1066074

22

2513

296.7

33.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.456 ± 0.044

1.619 ± 0.018

5.491 ± 0.03

7.402 ± 0.043

4.006 ± 0.025

7.862 ± 0.044

1.749 ± 0.017

5.768 ± 0.033

5.4 ± 0.043

9.797 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.854 ± 0.02

3.595 ± 0.028

4.245 ± 0.03

3.313 ± 0.025

5.699 ± 0.048

5.941 ± 0.033

5.245 ± 0.042

6.747 ± 0.033

1.107 ± 0.015

3.705 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski