Torque teno midi virus 15
Average proteome isoelectric point is 7.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B6ZK29|B6ZK29_9VIRU Capsid protein OS=Torque teno midi virus 15 OX=2065056 PE=3 SV=1
MM1 pKa = 6.75 QHH3 pKa = 6.0 YY4 pKa = 7.81 PPPKK8 pKa = 9.36 LYY10 pKa = 10.5 KK11 pKa = 10.15 IPFLDD16 pKa = 3.2 AWGKK20 pKa = 6.09 QQQWISSVEE29 pKa = 4.11 SQHH32 pKa = 7.15 DD33 pKa = 3.83 LWCGCHH39 pKa = 5.72 HH40 pKa = 7.25 WFAHH44 pKa = 5.72 FLDD47 pKa = 4.22 SVVPLDD53 pKa = 3.3 SRR55 pKa = 11.84 NRR57 pKa = 11.84 GKK59 pKa = 9.17 TIQEE63 pKa = 4.05 IIEE66 pKa = 4.19 QSTHH70 pKa = 5.2 QLRR73 pKa = 11.84 ALPALPLKK81 pKa = 9.59 PWPGDD86 pKa = 3.36 TTGDD90 pKa = 3.53 EE91 pKa = 4.46 KK92 pKa = 11.46 DD93 pKa = 3.53 PSEE96 pKa = 4.46 DD97 pKa = 3.25 AGKK100 pKa = 10.53 EE101 pKa = 4.05 EE102 pKa = 4.27 PQNTEE107 pKa = 3.62 NQDD110 pKa = 3.37 FTDD113 pKa = 4.0 VMPEE117 pKa = 3.98 DD118 pKa = 3.94 EE119 pKa = 4.46 LAEE122 pKa = 4.1 ILEE125 pKa = 4.32 NAARR129 pKa = 11.84 DD130 pKa = 3.85 EE131 pKa = 4.27 NVPGG135 pKa = 3.84
Molecular weight: 15.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.581
IPC2_protein 4.558
IPC_protein 4.482
Toseland 4.342
ProMoST 4.609
Dawson 4.444
Bjellqvist 4.584
Wikipedia 4.329
Rodwell 4.329
Grimsley 4.253
Solomon 4.444
Lehninger 4.393
Nozaki 4.558
DTASelect 4.724
Thurlkill 4.342
EMBOSS 4.342
Sillero 4.609
Patrickios 3.846
IPC_peptide 4.444
IPC2_peptide 4.596
IPC2.peptide.svr19 4.539
Protein with the highest isoelectric point:
>tr|B6ZK29|B6ZK29_9VIRU Capsid protein OS=Torque teno midi virus 15 OX=2065056 PE=3 SV=1
MM1 pKa = 7.48 AWRR4 pKa = 11.84 YY5 pKa = 5.97 YY6 pKa = 8.56 WRR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.27 RR11 pKa = 11.84 PFRR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 WKK18 pKa = 10.42 RR19 pKa = 11.84 RR20 pKa = 11.84 ATKK23 pKa = 9.44 YY24 pKa = 10.06 RR25 pKa = 11.84 KK26 pKa = 7.96 PRR28 pKa = 11.84 LYY30 pKa = 9.92 RR31 pKa = 11.84 RR32 pKa = 11.84 YY33 pKa = 9.4 ARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 TRR39 pKa = 11.84 RR40 pKa = 11.84 NPRR43 pKa = 11.84 KK44 pKa = 9.15 RR45 pKa = 11.84 RR46 pKa = 11.84 QRR48 pKa = 11.84 RR49 pKa = 11.84 KK50 pKa = 8.74 RR51 pKa = 11.84 PRR53 pKa = 11.84 VRR55 pKa = 11.84 RR56 pKa = 11.84 KK57 pKa = 9.95 RR58 pKa = 11.84 QTLPLKK64 pKa = 9.48 QWQPEE69 pKa = 4.26 SIRR72 pKa = 11.84 RR73 pKa = 11.84 CKK75 pKa = 10.37 IIGHH79 pKa = 5.37 TPIILGCSDD88 pKa = 3.91 RR89 pKa = 11.84 QIHH92 pKa = 6.93 SYY94 pKa = 9.45 TYY96 pKa = 7.64 WQDD99 pKa = 3.53 YY100 pKa = 9.34 YY101 pKa = 10.67 IHH103 pKa = 6.89 PRR105 pKa = 11.84 CAAGGGFAVMKK116 pKa = 10.21 WSLAYY121 pKa = 10.39 LYY123 pKa = 10.82 EE124 pKa = 4.36 DD125 pKa = 4.1 FKK127 pKa = 11.11 RR128 pKa = 11.84 RR129 pKa = 11.84 KK130 pKa = 10.02 NIWTKK135 pKa = 9.86 SNCMYY140 pKa = 10.68 DD141 pKa = 3.59 LCRR144 pKa = 11.84 YY145 pKa = 7.7 TGTKK149 pKa = 9.77 LKK151 pKa = 10.26 LYY153 pKa = 10.15 KK154 pKa = 10.32 HH155 pKa = 5.67 EE156 pKa = 4.17 TVDD159 pKa = 4.55 FIVSFSRR166 pKa = 11.84 NYY168 pKa = 10.42 PMVVDD173 pKa = 4.52 EE174 pKa = 4.26 NTYY177 pKa = 8.91 MHH179 pKa = 6.52 THH181 pKa = 6.56 PSIMLLKK188 pKa = 9.3 KK189 pKa = 9.86 HH190 pKa = 6.74 RR191 pKa = 11.84 IIIPSKK197 pKa = 8.67 RR198 pKa = 11.84 TNPHH202 pKa = 4.57 GKK204 pKa = 8.9 NYY206 pKa = 10.62 KK207 pKa = 9.93 KK208 pKa = 9.4 ITVRR212 pKa = 11.84 PPRR215 pKa = 11.84 QLINKK220 pKa = 8.14 WFFQKK225 pKa = 10.64 NFNDD229 pKa = 3.08 QGLFLLTATAADD241 pKa = 3.76 LMYY244 pKa = 10.22 PHH246 pKa = 7.58 LGPYY250 pKa = 9.49 SKK252 pKa = 10.81 NRR254 pKa = 11.84 LVTITILNTQHH265 pKa = 7.55 FYY267 pKa = 10.38 QQPNWGTATGDD278 pKa = 3.55 YY279 pKa = 11.2 SFGTTNFSTWGGAKK293 pKa = 9.58 TYY295 pKa = 10.38 IDD297 pKa = 4.67 NKK299 pKa = 9.38 LTDD302 pKa = 4.46 YY303 pKa = 10.68 NPSMDD308 pKa = 4.16 MLKK311 pKa = 10.58 QNNKK315 pKa = 9.24 HH316 pKa = 5.63 NASNGIFNKK325 pKa = 9.73 EE326 pKa = 3.98 FPNIQYY332 pKa = 8.84 WKK334 pKa = 8.81 TITNPTHH341 pKa = 6.31 PTPTMQVQYY350 pKa = 11.2 NPARR354 pKa = 11.84 DD355 pKa = 3.43 TGNGNKK361 pKa = 9.63 VYY363 pKa = 10.32 FISTLQTSWAPSTTDD378 pKa = 5.25 KK379 pKa = 11.43 VMTWTGEE386 pKa = 4.14 PLWLQLYY393 pKa = 10.32 GLTDD397 pKa = 4.0 YY398 pKa = 10.79 ILKK401 pKa = 10.8 VKK403 pKa = 8.84 GTSSALQDD411 pKa = 4.53 AICVIEE417 pKa = 4.25 SPYY420 pKa = 10.31 IEE422 pKa = 4.6 PQTHH426 pKa = 5.21 NKK428 pKa = 9.74 KK429 pKa = 10.31 FVFVGNPFMNGLTEE443 pKa = 5.21 CGNQPPGWLLSKK455 pKa = 10.2 WFLTLEE461 pKa = 4.1 DD462 pKa = 3.99 QMPILNEE469 pKa = 4.08 IIKK472 pKa = 10.22 SGPYY476 pKa = 9.34 VARR479 pKa = 11.84 QDD481 pKa = 3.86 GPRR484 pKa = 11.84 CTWEE488 pKa = 3.6 LHH490 pKa = 5.4 CDD492 pKa = 3.22 YY493 pKa = 11.09 RR494 pKa = 11.84 SFFKK498 pKa = 10.3 WGGAQPPLQNICDD511 pKa = 3.76 PHH513 pKa = 7.56 LQEE516 pKa = 4.76 TFPVPDD522 pKa = 3.79 TVKK525 pKa = 9.29 QTIQIIDD532 pKa = 3.74 PQKK535 pKa = 10.58 QIPEE539 pKa = 4.37 TLFHH543 pKa = 5.97 SWDD546 pKa = 3.44 YY547 pKa = 11.26 RR548 pKa = 11.84 RR549 pKa = 11.84 GLITKK554 pKa = 7.58 TALKK558 pKa = 10.42 RR559 pKa = 11.84 MYY561 pKa = 10.8 KK562 pKa = 10.22 NISDD566 pKa = 4.49 ADD568 pKa = 3.78 SSSTDD573 pKa = 3.09 GSEE576 pKa = 5.06 KK577 pKa = 10.62 SDD579 pKa = 3.43 TPRR582 pKa = 11.84 KK583 pKa = 8.77 KK584 pKa = 10.15 RR585 pKa = 11.84 KK586 pKa = 9.9 RR587 pKa = 11.84 GDD589 pKa = 3.54 PPCPEE594 pKa = 4.28 EE595 pKa = 4.27 KK596 pKa = 10.4 DD597 pKa = 3.34 NYY599 pKa = 10.0 IIQNLQEE606 pKa = 4.12 ICQAPQEE613 pKa = 4.33 TSGDD617 pKa = 3.83 LEE619 pKa = 4.29 QQLHH623 pKa = 5.56 NLRR626 pKa = 11.84 EE627 pKa = 3.92 QQLNKK632 pKa = 10.51 NISLLKK638 pKa = 10.1 LLAMLKK644 pKa = 9.91 QKK646 pKa = 9.98 QLQMQLAMGMMEE658 pKa = 4.08
Molecular weight: 77.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.219
IPC2_protein 9.458
IPC_protein 9.502
Toseland 10.058
ProMoST 9.765
Dawson 10.262
Bjellqvist 9.955
Wikipedia 10.438
Rodwell 10.672
Grimsley 10.335
Solomon 10.277
Lehninger 10.248
Nozaki 10.072
DTASelect 9.94
Thurlkill 10.116
EMBOSS 10.467
Sillero 10.189
Patrickios 10.101
IPC_peptide 10.277
IPC2_peptide 8.712
IPC2.peptide.svr19 8.421
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1329
135
658
332.3
38.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.117 ± 0.87
1.505 ± 0.177
5.267 ± 0.62
5.342 ± 1.113
3.085 ± 0.382
4.515 ± 0.359
3.386 ± 0.441
5.267 ± 0.439
8.126 ± 0.585
7.449 ± 0.498
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.107 ± 0.433
4.59 ± 0.635
7.976 ± 1.051
6.922 ± 0.289
6.622 ± 0.837
6.02 ± 1.316
7.299 ± 0.61
3.01 ± 0.338
2.784 ± 0.231
3.612 ± 0.833
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here