Sweet potato leaf curl Georgia virus-[16]
Average proteome isoelectric point is 7.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8V5Z8|Q8V5Z8_9GEMI Protein V2 OS=Sweet potato leaf curl Georgia virus-[16] OX=223330 GN=AV2 PE=3 SV=1
MM1 pKa = 7.75 AEE3 pKa = 4.4 LWDD6 pKa = 4.26 PLQNPLPDD14 pKa = 3.26 TLYY17 pKa = 11.06 GFRR20 pKa = 11.84 CMLSVKK26 pKa = 9.83 YY27 pKa = 10.41 LQGILKK33 pKa = 9.89 KK34 pKa = 10.45 YY35 pKa = 10.35 EE36 pKa = 4.24 PGTLGFEE43 pKa = 4.51 LCSEE47 pKa = 5.38 LIRR50 pKa = 11.84 IFRR53 pKa = 11.84 VRR55 pKa = 11.84 QYY57 pKa = 11.69 DD58 pKa = 3.54 RR59 pKa = 11.84 ANSRR63 pKa = 11.84 FAEE66 pKa = 4.36 ISSLWGEE73 pKa = 4.2 TGKK76 pKa = 8.6 TEE78 pKa = 4.27 AEE80 pKa = 3.93 LRR82 pKa = 11.84 DD83 pKa = 4.14 SYY85 pKa = 11.36 RR86 pKa = 11.84 ALHH89 pKa = 6.22 WEE91 pKa = 4.38 CCPNCCPKK99 pKa = 10.53 LCPGFKK105 pKa = 9.88 RR106 pKa = 11.84 RR107 pKa = 11.84 PDD109 pKa = 3.47 EE110 pKa = 4.28 EE111 pKa = 4.75 KK112 pKa = 10.81 EE113 pKa = 4.18 GG114 pKa = 3.68
Molecular weight: 13.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.993
IPC2_protein 6.186
IPC_protein 6.275
Toseland 6.033
ProMoST 6.664
Dawson 6.722
Bjellqvist 6.576
Wikipedia 6.664
Rodwell 6.707
Grimsley 6.008
Solomon 6.751
Lehninger 6.766
Nozaki 6.912
DTASelect 6.971
Thurlkill 6.985
EMBOSS 7.015
Sillero 7.132
Patrickios 3.897
IPC_peptide 6.766
IPC2_peptide 6.781
IPC2.peptide.svr19 6.683
Protein with the highest isoelectric point:
>tr|Q8V5Z8|Q8V5Z8_9GEMI Protein V2 OS=Sweet potato leaf curl Georgia virus-[16] OX=223330 GN=AV2 PE=3 SV=1
MM1 pKa = 7.54 TGRR4 pKa = 11.84 IPVSRR9 pKa = 11.84 RR10 pKa = 11.84 FHH12 pKa = 6.81 PYY14 pKa = 8.37 GGRR17 pKa = 11.84 PVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 LNFEE26 pKa = 3.93 TAIVPYY32 pKa = 9.22 TGNAVPIAARR42 pKa = 11.84 SYY44 pKa = 8.64 VPVSRR49 pKa = 11.84 GVRR52 pKa = 11.84 MKK54 pKa = 10.51 RR55 pKa = 11.84 KK56 pKa = 9.94 RR57 pKa = 11.84 GDD59 pKa = 4.09 RR60 pKa = 11.84 IPKK63 pKa = 9.95 GCVGPCKK70 pKa = 10.08 VQDD73 pKa = 3.86 YY74 pKa = 8.72 EE75 pKa = 4.55 FKK77 pKa = 10.29 MDD79 pKa = 3.56 VPHH82 pKa = 7.05 TGTFVCVSDD91 pKa = 3.96 FTRR94 pKa = 11.84 GTGLTHH100 pKa = 7.28 RR101 pKa = 11.84 LGKK104 pKa = 9.86 RR105 pKa = 11.84 VCIKK109 pKa = 11.05 SMGIDD114 pKa = 3.27 GKK116 pKa = 11.25 VWMDD120 pKa = 3.75 DD121 pKa = 3.28 NVAKK125 pKa = 10.24 RR126 pKa = 11.84 DD127 pKa = 3.48 HH128 pKa = 6.56 TNIITYY134 pKa = 9.11 WLVRR138 pKa = 11.84 DD139 pKa = 4.39 RR140 pKa = 11.84 RR141 pKa = 11.84 PNMDD145 pKa = 4.52 PLTFAQAFTMYY156 pKa = 10.75 DD157 pKa = 3.66 NEE159 pKa = 4.15 PTTAKK164 pKa = 10.17 IRR166 pKa = 11.84 MDD168 pKa = 3.48 LRR170 pKa = 11.84 DD171 pKa = 3.42 RR172 pKa = 11.84 MQVLKK177 pKa = 10.39 KK178 pKa = 10.29 FSVTVSGGPYY188 pKa = 8.51 NHH190 pKa = 7.45 KK191 pKa = 10.06 EE192 pKa = 3.61 QALVRR197 pKa = 11.84 KK198 pKa = 8.79 FFKK201 pKa = 10.63 GLYY204 pKa = 8.96 NHH206 pKa = 5.46 VTYY209 pKa = 10.87 NHH211 pKa = 6.65 KK212 pKa = 10.86 EE213 pKa = 3.59 EE214 pKa = 4.51 AKK216 pKa = 10.79 YY217 pKa = 10.68 EE218 pKa = 4.05 NHH220 pKa = 7.03 LEE222 pKa = 4.07 NALMLYY228 pKa = 9.88 SASSHH233 pKa = 6.23 ASNPVYY239 pKa = 9.46 QTLRR243 pKa = 11.84 CRR245 pKa = 11.84 AYY247 pKa = 10.58 FYY249 pKa = 10.72 DD250 pKa = 3.51 SHH252 pKa = 7.95 NNN254 pKa = 3.3
Molecular weight: 29.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.269
IPC2_protein 9.502
IPC_protein 9.721
Toseland 10.028
ProMoST 9.794
Dawson 10.262
Bjellqvist 9.984
Wikipedia 10.467
Rodwell 10.555
Grimsley 10.35
Solomon 10.292
Lehninger 10.248
Nozaki 10.058
DTASelect 9.97
Thurlkill 10.101
EMBOSS 10.438
Sillero 10.189
Patrickios 9.882
IPC_peptide 10.292
IPC2_peptide 8.873
IPC2.peptide.svr19 8.457
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1111
85
364
185.2
21.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.581 ± 0.843
2.79 ± 0.888
4.95 ± 0.553
5.131 ± 0.815
4.41 ± 0.704
5.761 ± 0.567
3.42 ± 0.391
5.581 ± 0.927
6.301 ± 0.349
7.201 ± 1.314
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.07 ± 0.713
4.95 ± 0.636
6.391 ± 0.84
4.68 ± 0.841
6.391 ± 1.179
8.461 ± 1.395
5.221 ± 1.377
5.491 ± 0.96
1.8 ± 0.363
3.42 ± 0.657
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here