Torque teno midi virus 4
Average proteome isoelectric point is 7.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8DMP4|A8DMP4_9VIRU Capsid protein OS=Torque teno midi virus 4 OX=2065045 PE=3 SV=1
MM1 pKa = 6.84 STFYY5 pKa = 10.79 KK6 pKa = 10.09 PSVYY10 pKa = 10.47 NGPTKK15 pKa = 9.96 EE16 pKa = 4.04 QMWMSVVTDD25 pKa = 3.81 FHH27 pKa = 8.55 DD28 pKa = 3.71 SFCRR32 pKa = 11.84 CFHH35 pKa = 6.32 SFAHH39 pKa = 6.35 ILDD42 pKa = 5.26 LIFPDD47 pKa = 3.58 GHH49 pKa = 6.94 RR50 pKa = 11.84 DD51 pKa = 3.19 RR52 pKa = 11.84 DD53 pKa = 3.48 KK54 pKa = 10.78 TIRR57 pKa = 11.84 EE58 pKa = 3.94 IIEE61 pKa = 4.48 RR62 pKa = 11.84 DD63 pKa = 3.87 LKK65 pKa = 10.69 CHH67 pKa = 6.36 SGGDD71 pKa = 3.55 EE72 pKa = 3.96 EE73 pKa = 4.54 EE74 pKa = 4.41 RR75 pKa = 11.84 CGGVLAAAIEE85 pKa = 4.11 NTEE88 pKa = 4.35 NINQEE93 pKa = 4.01 EE94 pKa = 4.38 SQDD97 pKa = 3.46 IADD100 pKa = 3.68 ADD102 pKa = 3.69 IRR104 pKa = 11.84 EE105 pKa = 4.29 LLAAAEE111 pKa = 4.27 SAEE114 pKa = 3.98 RR115 pKa = 11.84 RR116 pKa = 3.81
Molecular weight: 13.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.714
IPC2_protein 4.736
IPC_protein 4.635
Toseland 4.52
ProMoST 4.762
Dawson 4.596
Bjellqvist 4.749
Wikipedia 4.469
Rodwell 4.495
Grimsley 4.431
Solomon 4.596
Lehninger 4.558
Nozaki 4.711
DTASelect 4.863
Thurlkill 4.52
EMBOSS 4.495
Sillero 4.774
Patrickios 3.872
IPC_peptide 4.609
IPC2_peptide 4.762
IPC2.peptide.svr19 4.725
Protein with the highest isoelectric point:
>tr|A8DMP5|A8DMP5_9VIRU ORF3 OS=Torque teno midi virus 4 OX=2065045 PE=4 SV=1
MM1 pKa = 7.27 TEE3 pKa = 3.96 IVLGNYY9 pKa = 10.05 LITIFSILSGEE20 pKa = 4.38 DD21 pKa = 3.31 QQHH24 pKa = 6.16 HH25 pKa = 5.59 NKK27 pKa = 9.92 KK28 pKa = 10.17 LATPTPKK35 pKa = 10.19 TSTLFPILSKK45 pKa = 10.6 EE46 pKa = 3.89 QYY48 pKa = 10.32 KK49 pKa = 10.31 YY50 pKa = 8.1 PTHH53 pKa = 6.68 SNKK56 pKa = 9.93 ALKK59 pKa = 10.15 AYY61 pKa = 7.41 STTGILEE68 pKa = 4.24 EE69 pKa = 5.26 GSLQKK74 pKa = 10.68 QLLKK78 pKa = 10.82 EE79 pKa = 4.0 CAKK82 pKa = 9.35 TSKK85 pKa = 10.51 LIQISSQIRR94 pKa = 11.84 KK95 pKa = 8.64 RR96 pKa = 11.84 HH97 pKa = 4.27 QQKK100 pKa = 10.68 DD101 pKa = 3.45 QDD103 pKa = 3.81 WQEE106 pKa = 3.62 NSYY109 pKa = 9.48 TPEE112 pKa = 3.76 EE113 pKa = 4.03 KK114 pKa = 10.31 KK115 pKa = 10.42 QRR117 pKa = 11.84 NQDD120 pKa = 3.49 MSPGSLQRR128 pKa = 11.84 RR129 pKa = 11.84 YY130 pKa = 10.36 LPRR133 pKa = 11.84 NTTDD137 pKa = 2.56 TRR139 pKa = 11.84 RR140 pKa = 11.84 NHH142 pKa = 6.65 PPHH145 pKa = 6.82 PAAARR150 pKa = 11.84 AAPPLRR156 pKa = 11.84 HH157 pKa = 6.03 RR158 pKa = 11.84 PRR160 pKa = 11.84 PRR162 pKa = 11.84 RR163 pKa = 11.84 HH164 pKa = 6.1 LGCGRR169 pKa = 11.84 AGAKK173 pKa = 8.62 MAGPNN178 pKa = 3.41
Molecular weight: 20.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.246
IPC2_protein 9.604
IPC_protein 9.794
Toseland 10.482
ProMoST 10.058
Dawson 10.599
Bjellqvist 10.233
Wikipedia 10.745
Rodwell 11.082
Grimsley 10.643
Solomon 10.643
Lehninger 10.613
Nozaki 10.452
DTASelect 10.233
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.511
Patrickios 10.789
IPC_peptide 10.643
IPC2_peptide 8.902
IPC2.peptide.svr19 8.64
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
952
116
658
317.3
36.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.357 ± 1.288
1.471 ± 0.568
3.676 ± 1.681
5.147 ± 1.853
4.307 ± 1.024
4.832 ± 0.155
2.941 ± 0.817
5.987 ± 0.501
8.613 ± 1.539
6.933 ± 0.688
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.366 ± 0.436
4.622 ± 0.383
7.563 ± 1.43
4.832 ± 0.984
7.668 ± 0.278
7.668 ± 0.314
7.038 ± 0.762
2.626 ± 0.652
1.891 ± 0.664
5.462 ± 1.606
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here