Paralcaligenes ureilyticus
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3977 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R3M6U0|A0A4R3M6U0_9BURK Microcin-processing peptidase 1 OS=Paralcaligenes ureilyticus OX=627131 GN=EDC26_104304 PE=3 SV=1
MM1 pKa = 7.03 QASNLPIVLIFGPFDD16 pKa = 3.84 PSGSSSLPADD26 pKa = 3.52 AVTCATLGCHH36 pKa = 6.64 ALSTLTAIRR45 pKa = 11.84 VQDD48 pKa = 3.47 TVGIEE53 pKa = 4.27 EE54 pKa = 4.43 IQPVAPEE61 pKa = 5.13 LIDD64 pKa = 4.11 DD65 pKa = 3.92 QARR68 pKa = 11.84 CLLEE72 pKa = 5.17 DD73 pKa = 3.78 MAVQAIKK80 pKa = 10.26 IGPLYY85 pKa = 10.12 TPEE88 pKa = 4.26 SVSALAQIAADD99 pKa = 3.87 YY100 pKa = 10.28 NQVPLVLHH108 pKa = 5.72 LTAWPDD114 pKa = 3.18 EE115 pKa = 4.23 AALEE119 pKa = 5.11 DD120 pKa = 4.07 IDD122 pKa = 5.78 SEE124 pKa = 4.62 DD125 pKa = 3.53 VLSATLEE132 pKa = 4.1 LLLPQTDD139 pKa = 3.47 IVIADD144 pKa = 4.08 HH145 pKa = 6.65 NLMVQWQAEE154 pKa = 4.43 GLLSTIDD161 pKa = 3.51 AASPAQALLQCGAKK175 pKa = 8.99 WVLSTAAPIRR185 pKa = 11.84 PGQSAYY191 pKa = 11.1 LLQGQEE197 pKa = 3.88 NEE199 pKa = 4.29 TFNWAWQAPAARR211 pKa = 11.84 LHH213 pKa = 6.95 DD214 pKa = 4.45 ADD216 pKa = 5.26 GPLACAVACQLAMGLDD232 pKa = 3.56 APQAVEE238 pKa = 4.12 AAIKK242 pKa = 10.12 QAMPLAANSFQPGMGHH258 pKa = 6.5 PLINRR263 pKa = 11.84 SPSS266 pKa = 2.98
Molecular weight: 28.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.791
IPC2_protein 3.999
IPC_protein 3.973
Toseland 3.77
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.872
Rodwell 3.808
Grimsley 3.681
Solomon 3.948
Lehninger 3.91
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.821
EMBOSS 3.884
Sillero 4.101
Patrickios 1.926
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 3.977
Protein with the highest isoelectric point:
>tr|A0A4R3LRY7|A0A4R3LRY7_9BURK Filamentous hemagglutinin family protein (Fragment) OS=Paralcaligenes ureilyticus OX=627131 GN=EDC26_1151 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.17 RR14 pKa = 11.84 THH16 pKa = 5.95 GFRR19 pKa = 11.84 IRR21 pKa = 11.84 MKK23 pKa = 9.4 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3977
0
3977
1301549
30
3014
327.3
35.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.573 ± 0.048
1.004 ± 0.014
5.12 ± 0.03
4.871 ± 0.039
3.701 ± 0.026
8.196 ± 0.049
2.349 ± 0.018
5.473 ± 0.03
3.633 ± 0.03
10.667 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.583 ± 0.019
3.003 ± 0.024
4.976 ± 0.024
3.984 ± 0.026
6.278 ± 0.033
5.931 ± 0.029
5.223 ± 0.031
7.533 ± 0.033
1.385 ± 0.018
2.516 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here