Capybara microvirus Cap1_SP_45
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVM4|A0A4V1FVM4_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_45 OX=2584778 PE=4 SV=1
MM1 pKa = 7.61 SNNNSQTINISKK13 pKa = 9.6 ASKK16 pKa = 9.78 YY17 pKa = 10.84 SRR19 pKa = 11.84 ISLDD23 pKa = 3.46 EE24 pKa = 3.98 AHH26 pKa = 6.81 LTTLDD31 pKa = 3.88 FGQIMPIYY39 pKa = 9.79 SRR41 pKa = 11.84 AIIPGDD47 pKa = 3.91 DD48 pKa = 3.68 LSIDD52 pKa = 3.7 TNVFTRR58 pKa = 11.84 TSPLVYY64 pKa = 9.73 PSFVNAHH71 pKa = 5.7 LTLVSVFVPYY81 pKa = 10.42 YY82 pKa = 9.17 QLSPFMEE89 pKa = 4.65 PFFAGVPNIGSKK101 pKa = 8.27 VVKK104 pKa = 10.52 LPLLTNHH111 pKa = 7.33 IIDD114 pKa = 4.81 HH115 pKa = 6.85 LFTDD119 pKa = 5.16 LAQTLNIGKK128 pKa = 9.59 IGQIGVNPDD137 pKa = 4.11 IIWTTTDD144 pKa = 2.81 NLKK147 pKa = 11.01 YY148 pKa = 9.68 GTQLNYY154 pKa = 10.4 KK155 pKa = 8.78 GRR157 pKa = 11.84 LMLKK161 pKa = 9.54 VLNSLGYY168 pKa = 9.38 QVSKK172 pKa = 10.6 NVSWKK177 pKa = 10.36 SSVDD181 pKa = 3.41 NEE183 pKa = 4.5 YY184 pKa = 11.14 KK185 pKa = 10.69 NSPVTLNPLPFISYY199 pKa = 10.0 IKK201 pKa = 10.43 VYY203 pKa = 10.75 KK204 pKa = 10.48 DD205 pKa = 2.82 WFSSNKK211 pKa = 9.88 SFNTSYY217 pKa = 10.82 VASILNRR224 pKa = 11.84 IQTEE228 pKa = 3.99 SDD230 pKa = 3.78 FEE232 pKa = 4.1 FTPQIINSLFDD243 pKa = 3.98 YY244 pKa = 10.91 LRR246 pKa = 11.84 LLYY249 pKa = 9.73 DD250 pKa = 3.18 TDD252 pKa = 4.06 YY253 pKa = 9.57 FTTAWQYY260 pKa = 11.58 NNYY263 pKa = 9.56 PSDD266 pKa = 3.7 SSKK269 pKa = 10.17 TSTDD273 pKa = 3.06 ILPDD277 pKa = 4.05 PSWSGLYY284 pKa = 10.29 DD285 pKa = 3.45 GNRR288 pKa = 11.84 FLINPNEE295 pKa = 3.98 NLADD299 pKa = 3.61 QSDD302 pKa = 4.27 PEE304 pKa = 4.31 FLGATTVRR312 pKa = 11.84 LLSVLDD318 pKa = 3.54 KK319 pKa = 10.72 YY320 pKa = 10.89 IRR322 pKa = 11.84 RR323 pKa = 11.84 NSYY326 pKa = 10.99 AGTKK330 pKa = 9.28 AAARR334 pKa = 11.84 VLSRR338 pKa = 11.84 FGIKK342 pKa = 8.54 STEE345 pKa = 4.11 FKK347 pKa = 10.69 SQYY350 pKa = 10.89 SDD352 pKa = 2.79 ILNVDD357 pKa = 4.6 SIRR360 pKa = 11.84 LNIGDD365 pKa = 3.8 VTSTAEE371 pKa = 4.11 TTEE374 pKa = 4.2 MPLGSYY380 pKa = 9.52 AGKK383 pKa = 10.47 AILGGKK389 pKa = 9.59 GGAKK393 pKa = 9.89 LKK395 pKa = 11.21 SNDD398 pKa = 3.18 FGHH401 pKa = 6.52 FFVIAYY407 pKa = 9.41 ISAKK411 pKa = 8.32 TCYY414 pKa = 9.5 PSGFDD419 pKa = 3.61 RR420 pKa = 11.84 EE421 pKa = 4.42 CLKK424 pKa = 10.91 SHH426 pKa = 7.18 LYY428 pKa = 10.7 DD429 pKa = 4.8 FYY431 pKa = 11.04 TPEE434 pKa = 3.94 FDD436 pKa = 5.07 GVGLQPISMQEE447 pKa = 3.81 LSSEE451 pKa = 4.16 NLNFTYY457 pKa = 10.45 SEE459 pKa = 4.22 PWHH462 pKa = 6.46 GNDD465 pKa = 3.07 VFGFTEE471 pKa = 4.84 RR472 pKa = 11.84 YY473 pKa = 8.5 NEE475 pKa = 3.72 YY476 pKa = 10.6 RR477 pKa = 11.84 FGKK480 pKa = 10.24 DD481 pKa = 2.76 RR482 pKa = 11.84 ITGDD486 pKa = 3.49 MLRR489 pKa = 11.84 DD490 pKa = 3.31 MDD492 pKa = 4.68 FKK494 pKa = 10.54 PWYY497 pKa = 9.94 LSRR500 pKa = 11.84 EE501 pKa = 4.16 DD502 pKa = 3.61 LTIRR506 pKa = 11.84 DD507 pKa = 3.69 QDD509 pKa = 3.49 VTAQSDD515 pKa = 3.66 PLLYY519 pKa = 10.79 YY520 pKa = 9.69 PINNEE525 pKa = 3.41 SSYY528 pKa = 11.07 TRR530 pKa = 11.84 IFANTSSTLYY540 pKa = 10.28 DD541 pKa = 3.52 HH542 pKa = 7.53 RR543 pKa = 11.84 DD544 pKa = 3.32 HH545 pKa = 7.27 FFMSCYY551 pKa = 10.09 FNVSGTRR558 pKa = 11.84 PILNLSDD565 pKa = 4.02 SLSLGHH571 pKa = 7.41 GDD573 pKa = 3.14 INLNKK578 pKa = 10.04 EE579 pKa = 4.17 GLVLSS584 pKa = 4.53
Molecular weight: 66.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.189
IPC2_protein 5.27
IPC_protein 5.27
Toseland 5.397
ProMoST 5.499
Dawson 5.372
Bjellqvist 5.461
Wikipedia 5.321
Rodwell 5.296
Grimsley 5.397
Solomon 5.372
Lehninger 5.347
Nozaki 5.55
DTASelect 5.766
Thurlkill 5.474
EMBOSS 5.486
Sillero 5.614
Patrickios 3.656
IPC_peptide 5.385
IPC2_peptide 5.614
IPC2.peptide.svr19 5.574
Protein with the highest isoelectric point:
>tr|A0A4P8W3U8|A0A4P8W3U8_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_45 OX=2584778 PE=3 SV=1
MM1 pKa = 7.72 SYY3 pKa = 9.2 TFKK6 pKa = 11.16 VEE8 pKa = 4.05 NKK10 pKa = 6.67 THH12 pKa = 6.01 YY13 pKa = 10.48 FGEE16 pKa = 4.16 NTKK19 pKa = 10.64 LYY21 pKa = 10.74 NNIVNPRR28 pKa = 11.84 SYY30 pKa = 11.26 AALHH34 pKa = 5.96 SKK36 pKa = 9.48 QLSYY40 pKa = 10.79 YY41 pKa = 8.23 VRR43 pKa = 11.84 SYY45 pKa = 12.0 YY46 pKa = 10.67 EE47 pKa = 3.81 FLKK50 pKa = 10.23 TKK52 pKa = 9.72 YY53 pKa = 10.69 LNGKK57 pKa = 6.9 TMFLTFTYY65 pKa = 10.02 CDD67 pKa = 3.18 NALPILYY74 pKa = 9.51 GIPTVDD80 pKa = 3.47 NNNIRR85 pKa = 11.84 YY86 pKa = 9.01 LLRR89 pKa = 11.84 NSYY92 pKa = 10.14 LPKK95 pKa = 9.92 FVASKK100 pKa = 9.81 GYY102 pKa = 9.97 KK103 pKa = 7.17 MSYY106 pKa = 9.23 MIVPEE111 pKa = 4.08 FGEE114 pKa = 4.79 GKK116 pKa = 10.21 GSRR119 pKa = 11.84 GVGNNPHH126 pKa = 5.96 FHH128 pKa = 6.89 CLLHH132 pKa = 6.47 FYY134 pKa = 10.67 KK135 pKa = 10.18 EE136 pKa = 4.21 TGEE139 pKa = 4.07 PFAYY143 pKa = 10.31 ADD145 pKa = 4.59 GVDD148 pKa = 3.86 IYY150 pKa = 11.9 NKK152 pKa = 9.89 ILNLWQGTNPDD163 pKa = 3.32 GTITRR168 pKa = 11.84 DD169 pKa = 3.11 PLKK172 pKa = 10.21 YY173 pKa = 10.23 HH174 pKa = 6.82 FGMVQASANGLYY186 pKa = 9.86 IDD188 pKa = 5.27 DD189 pKa = 4.75 YY190 pKa = 10.71 KK191 pKa = 11.34 ACSYY195 pKa = 9.92 VSKK198 pKa = 9.88 YY199 pKa = 7.85 TSKK202 pKa = 11.11 DD203 pKa = 2.7 INTRR207 pKa = 11.84 TKK209 pKa = 9.95 ISQIEE214 pKa = 3.99 KK215 pKa = 10.4 AIEE218 pKa = 4.01 LDD220 pKa = 3.62 CFHH223 pKa = 7.02 YY224 pKa = 9.95 CTDD227 pKa = 4.26 SSICDD232 pKa = 3.08 IMNSLNVSDD241 pKa = 4.64 SLGSYY246 pKa = 7.74 LHH248 pKa = 6.73 KK249 pKa = 10.59 KK250 pKa = 9.27 YY251 pKa = 10.91 LSDD254 pKa = 4.07 DD255 pKa = 3.42 SPYY258 pKa = 10.97 RR259 pKa = 11.84 KK260 pKa = 10.24 DD261 pKa = 3.02 FEE263 pKa = 4.53 KK264 pKa = 10.53 RR265 pKa = 11.84 VKK267 pKa = 10.54 QMSAVIFADD276 pKa = 3.0 IKK278 pKa = 10.96 NRR280 pKa = 11.84 YY281 pKa = 7.47 LPKK284 pKa = 9.77 VFMSKK289 pKa = 10.46 GYY291 pKa = 10.98 GDD293 pKa = 3.66 YY294 pKa = 11.37 ALNFVNEE301 pKa = 4.36 DD302 pKa = 3.28 FKK304 pKa = 11.83 LPIPQSNPNAKK315 pKa = 9.44 VQGTIFIDD323 pKa = 3.75 LPLYY327 pKa = 10.53 LYY329 pKa = 10.49 RR330 pKa = 11.84 KK331 pKa = 9.93 LFTDD335 pKa = 2.89 VHH337 pKa = 7.76 KK338 pKa = 10.81 DD339 pKa = 3.09 EE340 pKa = 5.26 KK341 pKa = 11.47 GNVIYY346 pKa = 10.74 VLNDD350 pKa = 3.18 TGKK353 pKa = 9.42 HH354 pKa = 4.25 YY355 pKa = 11.34 KK356 pKa = 7.94 NTRR359 pKa = 11.84 LLKK362 pKa = 10.77 DD363 pKa = 3.0 IDD365 pKa = 3.85 KK366 pKa = 9.62 TCNDD370 pKa = 3.8 VACLISDD377 pKa = 4.11 NFDD380 pKa = 3.36 MPLDD384 pKa = 3.77 QLSSLSYY391 pKa = 10.54 DD392 pKa = 3.32 YY393 pKa = 11.51 SLYY396 pKa = 9.61 KK397 pKa = 10.2 HH398 pKa = 6.66 IYY400 pKa = 7.11 EE401 pKa = 4.45 HH402 pKa = 6.24 HH403 pKa = 6.62 TFDD406 pKa = 3.84 IFQPHH411 pKa = 6.34 EE412 pKa = 4.49 LDD414 pKa = 3.4 PLGDD418 pKa = 3.53 YY419 pKa = 10.47 TRR421 pKa = 11.84 FLVSGFEE428 pKa = 4.18 TEE430 pKa = 4.26 SFEE433 pKa = 4.4 EE434 pKa = 4.96 FYY436 pKa = 10.77 FVKK439 pKa = 10.17 KK440 pKa = 10.47 RR441 pKa = 11.84 KK442 pKa = 9.17 LLQSRR447 pKa = 11.84 LTYY450 pKa = 10.1 EE451 pKa = 3.75 FHH453 pKa = 7.63 PYY455 pKa = 9.17 FAPLIPYY462 pKa = 9.08 FRR464 pKa = 11.84 IIDD467 pKa = 3.8 NFVDD471 pKa = 3.67 NLNSEE476 pKa = 4.23 NDD478 pKa = 3.12 KK479 pKa = 11.27 ALYY482 pKa = 10.42 DD483 pKa = 3.72 NYY485 pKa = 11.29 LEE487 pKa = 4.61 IIRR490 pKa = 11.84 LRR492 pKa = 11.84 KK493 pKa = 9.11 VHH495 pKa = 6.43 NKK497 pKa = 8.19 MLL499 pKa = 4.26
Molecular weight: 58.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.119
IPC2_protein 7.351
IPC_protein 7.263
Toseland 7.117
ProMoST 7.775
Dawson 7.936
Bjellqvist 8.141
Wikipedia 7.878
Rodwell 7.951
Grimsley 7.22
Solomon 7.995
Lehninger 8.009
Nozaki 8.2
DTASelect 8.024
Thurlkill 8.068
EMBOSS 8.156
Sillero 8.317
Patrickios 3.452
IPC_peptide 7.995
IPC2_peptide 7.234
IPC2.peptide.svr19 7.414
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1696
101
584
339.2
38.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.894 ± 1.149
1.179 ± 0.539
6.958 ± 0.319
4.127 ± 0.576
5.483 ± 0.645
5.425 ± 0.461
2.358 ± 0.376
6.309 ± 0.39
6.663 ± 0.944
10.436 ± 0.351
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.005 ± 0.44
7.724 ± 0.478
4.068 ± 0.837
3.243 ± 0.927
3.656 ± 0.134
9.198 ± 1.266
5.307 ± 0.864
4.422 ± 0.624
0.884 ± 0.286
5.66 ± 1.395
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here