Amino acid dipepetide frequency for Bacillus phage vB_BsuS-Goe11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.497AlaAla: 3.497 ± 0.493
0.496AlaCys: 0.496 ± 0.125
3.277AlaAsp: 3.277 ± 0.276
3.359AlaGlu: 3.359 ± 0.294
2.175AlaPhe: 2.175 ± 0.233
2.726AlaGly: 2.726 ± 0.348
0.798AlaHis: 0.798 ± 0.167
4.103AlaIle: 4.103 ± 0.368
5.286AlaLys: 5.286 ± 0.505
4.323AlaLeu: 4.323 ± 0.539
1.322AlaMet: 1.322 ± 0.211
2.395AlaAsn: 2.395 ± 0.298
1.184AlaPro: 1.184 ± 0.189
1.652AlaGln: 1.652 ± 0.353
1.735AlaArg: 1.735 ± 0.182
3.359AlaSer: 3.359 ± 0.379
2.946AlaThr: 2.946 ± 0.258
3.029AlaVal: 3.029 ± 0.346
0.551AlaTrp: 0.551 ± 0.122
2.753AlaTyr: 2.753 ± 0.316
0.0AlaXaa: 0.0 ± 0.0
Cys
0.193CysAla: 0.193 ± 0.076
0.22CysCys: 0.22 ± 0.071
0.385CysAsp: 0.385 ± 0.095
0.854CysGlu: 0.854 ± 0.159
0.606CysPhe: 0.606 ± 0.1
0.661CysGly: 0.661 ± 0.144
0.11CysHis: 0.11 ± 0.056
0.688CysIle: 0.688 ± 0.141
0.798CysLys: 0.798 ± 0.17
1.019CysLeu: 1.019 ± 0.202
0.193CysMet: 0.193 ± 0.076
0.661CysAsn: 0.661 ± 0.139
0.165CysPro: 0.165 ± 0.063
0.193CysGln: 0.193 ± 0.08
0.33CysArg: 0.33 ± 0.102
0.578CysSer: 0.578 ± 0.128
0.606CysThr: 0.606 ± 0.147
0.716CysVal: 0.716 ± 0.159
0.165CysTrp: 0.165 ± 0.062
0.303CysTyr: 0.303 ± 0.082
0.0CysXaa: 0.0 ± 0.0
Asp
2.946AspAla: 2.946 ± 0.273
0.716AspCys: 0.716 ± 0.149
3.91AspAsp: 3.91 ± 0.377
5.644AspGlu: 5.644 ± 0.416
3.304AspPhe: 3.304 ± 0.264
4.571AspGly: 4.571 ± 0.458
0.909AspHis: 0.909 ± 0.185
5.424AspIle: 5.424 ± 0.384
5.259AspLys: 5.259 ± 0.484
5.479AspLeu: 5.479 ± 0.332
1.652AspMet: 1.652 ± 0.207
3.387AspAsn: 3.387 ± 0.329
1.707AspPro: 1.707 ± 0.212
2.148AspGln: 2.148 ± 0.249
2.258AspArg: 2.258 ± 0.284
4.295AspSer: 4.295 ± 0.358
2.781AspThr: 2.781 ± 0.257
3.992AspVal: 3.992 ± 0.349
0.578AspTrp: 0.578 ± 0.132
3.249AspTyr: 3.249 ± 0.303
0.0AspXaa: 0.0 ± 0.0
Glu
4.295GluAla: 4.295 ± 0.499
0.661GluCys: 0.661 ± 0.138
4.929GluAsp: 4.929 ± 0.414
7.049GluGlu: 7.049 ± 0.581
3.304GluPhe: 3.304 ± 0.386
3.249GluGly: 3.249 ± 0.391
1.349GluHis: 1.349 ± 0.193
6.939GluIle: 6.939 ± 0.465
7.517GluLys: 7.517 ± 0.494
7.654GluLeu: 7.654 ± 0.502
1.955GluMet: 1.955 ± 0.232
4.516GluAsn: 4.516 ± 0.4
1.459GluPro: 1.459 ± 0.204
3.221GluGln: 3.221 ± 0.344
3.497GluArg: 3.497 ± 0.329
3.992GluSer: 3.992 ± 0.29
4.35GluThr: 4.35 ± 0.334
5.342GluVal: 5.342 ± 0.443
1.211GluTrp: 1.211 ± 0.241
3.745GluTyr: 3.745 ± 0.355
0.0GluXaa: 0.0 ± 0.0
Phe
2.093PheAla: 2.093 ± 0.234
0.468PheCys: 0.468 ± 0.137
3.469PheAsp: 3.469 ± 0.288
4.295PheGlu: 4.295 ± 0.398
2.01PhePhe: 2.01 ± 0.314
2.175PheGly: 2.175 ± 0.251
0.936PheHis: 0.936 ± 0.169
3.056PheIle: 3.056 ± 0.335
4.378PheLys: 4.378 ± 0.4
3.277PheLeu: 3.277 ± 0.33
1.129PheMet: 1.129 ± 0.197
3.469PheAsn: 3.469 ± 0.313
1.459PhePro: 1.459 ± 0.203
0.798PheGln: 0.798 ± 0.147
1.349PheArg: 1.349 ± 0.156
2.671PheSer: 2.671 ± 0.266
2.423PheThr: 2.423 ± 0.279
2.203PheVal: 2.203 ± 0.253
0.358PheTrp: 0.358 ± 0.103
1.817PheTyr: 1.817 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
2.093GlyAla: 2.093 ± 0.224
0.606GlyCys: 0.606 ± 0.156
3.387GlyAsp: 3.387 ± 0.293
3.855GlyGlu: 3.855 ± 0.434
2.478GlyPhe: 2.478 ± 0.233
3.111GlyGly: 3.111 ± 0.331
1.156GlyHis: 1.156 ± 0.225
4.295GlyIle: 4.295 ± 0.447
4.681GlyLys: 4.681 ± 0.358
4.984GlyLeu: 4.984 ± 0.323
1.239GlyMet: 1.239 ± 0.176
3.469GlyAsn: 3.469 ± 0.324
0.138GlyPro: 0.138 ± 0.058
1.9GlyGln: 1.9 ± 0.213
2.038GlyArg: 2.038 ± 0.263
3.304GlySer: 3.304 ± 0.323
3.579GlyThr: 3.579 ± 0.302
3.469GlyVal: 3.469 ± 0.316
0.606GlyTrp: 0.606 ± 0.144
3.056GlyTyr: 3.056 ± 0.273
0.0GlyXaa: 0.0 ± 0.0
His
0.413HisAla: 0.413 ± 0.114
0.11HisCys: 0.11 ± 0.047
1.046HisAsp: 1.046 ± 0.171
1.514HisGlu: 1.514 ± 0.208
0.964HisPhe: 0.964 ± 0.17
1.101HisGly: 1.101 ± 0.185
0.33HisHis: 0.33 ± 0.079
1.459HisIle: 1.459 ± 0.21
1.817HisLys: 1.817 ± 0.234
1.432HisLeu: 1.432 ± 0.221
0.551HisMet: 0.551 ± 0.142
1.267HisAsn: 1.267 ± 0.176
0.578HisPro: 0.578 ± 0.116
0.523HisGln: 0.523 ± 0.113
0.633HisArg: 0.633 ± 0.128
1.349HisSer: 1.349 ± 0.197
0.964HisThr: 0.964 ± 0.168
1.156HisVal: 1.156 ± 0.182
0.165HisTrp: 0.165 ± 0.071
0.661HisTyr: 0.661 ± 0.123
0.0HisXaa: 0.0 ± 0.0
Ile
4.13IleAla: 4.13 ± 0.399
0.633IleCys: 0.633 ± 0.152
5.369IleAsp: 5.369 ± 0.401
6.278IleGlu: 6.278 ± 0.346
2.451IlePhe: 2.451 ± 0.272
3.965IleGly: 3.965 ± 0.358
1.707IleHis: 1.707 ± 0.216
4.35IleIle: 4.35 ± 0.421
8.398IleLys: 8.398 ± 0.463
4.736IleLeu: 4.736 ± 0.372
1.349IleMet: 1.349 ± 0.193
5.617IleAsn: 5.617 ± 0.473
2.203IlePro: 2.203 ± 0.258
2.588IleGln: 2.588 ± 0.256
2.864IleArg: 2.864 ± 0.307
5.424IleSer: 5.424 ± 0.485
4.213IleThr: 4.213 ± 0.41
3.965IleVal: 3.965 ± 0.346
0.854IleTrp: 0.854 ± 0.209
2.561IleTyr: 2.561 ± 0.232
0.0IleXaa: 0.0 ± 0.0
Lys
5.342LysAla: 5.342 ± 0.548
0.909LysCys: 0.909 ± 0.192
6.057LysAsp: 6.057 ± 0.397
8.508LysGlu: 8.508 ± 0.641
3.332LysPhe: 3.332 ± 0.307
5.011LysGly: 5.011 ± 0.395
1.872LysHis: 1.872 ± 0.229
6.14LysIle: 6.14 ± 0.417
9.444LysLys: 9.444 ± 0.635
8.48LysLeu: 8.48 ± 0.527
2.065LysMet: 2.065 ± 0.29
5.727LysAsn: 5.727 ± 0.448
2.34LysPro: 2.34 ± 0.279
3.855LysGln: 3.855 ± 0.424
4.103LysArg: 4.103 ± 0.481
6.526LysSer: 6.526 ± 0.499
5.204LysThr: 5.204 ± 0.527
6.195LysVal: 6.195 ± 0.497
0.854LysTrp: 0.854 ± 0.17
4.46LysTyr: 4.46 ± 0.404
0.0LysXaa: 0.0 ± 0.0
Leu
4.433LeuAla: 4.433 ± 0.394
0.798LeuCys: 0.798 ± 0.16
5.204LeuAsp: 5.204 ± 0.339
5.865LeuGlu: 5.865 ± 0.481
3.855LeuPhe: 3.855 ± 0.432
4.213LeuGly: 4.213 ± 0.31
1.68LeuHis: 1.68 ± 0.216
6.608LeuIle: 6.608 ± 0.483
8.205LeuLys: 8.205 ± 0.588
7.544LeuLeu: 7.544 ± 0.463
1.569LeuMet: 1.569 ± 0.237
6.057LeuAsn: 6.057 ± 0.505
2.313LeuPro: 2.313 ± 0.246
3.277LeuGln: 3.277 ± 0.512
3.414LeuArg: 3.414 ± 0.354
6.911LeuSer: 6.911 ± 0.464
5.011LeuThr: 5.011 ± 0.395
4.681LeuVal: 4.681 ± 0.347
0.606LeuTrp: 0.606 ± 0.15
3.992LeuTyr: 3.992 ± 0.428
0.0LeuXaa: 0.0 ± 0.0
Met
1.377MetAla: 1.377 ± 0.198
0.193MetCys: 0.193 ± 0.079
1.652MetAsp: 1.652 ± 0.211
1.542MetGlu: 1.542 ± 0.247
0.991MetPhe: 0.991 ± 0.18
1.101MetGly: 1.101 ± 0.206
0.303MetHis: 0.303 ± 0.081
1.624MetIle: 1.624 ± 0.207
2.313MetLys: 2.313 ± 0.238
2.203MetLeu: 2.203 ± 0.275
0.771MetMet: 0.771 ± 0.149
1.156MetAsn: 1.156 ± 0.21
0.688MetPro: 0.688 ± 0.119
0.936MetGln: 0.936 ± 0.214
0.826MetArg: 0.826 ± 0.122
1.624MetSer: 1.624 ± 0.201
1.735MetThr: 1.735 ± 0.214
0.716MetVal: 0.716 ± 0.129
0.275MetTrp: 0.275 ± 0.103
0.771MetTyr: 0.771 ± 0.125
0.0MetXaa: 0.0 ± 0.0
Asn
3.304AsnAla: 3.304 ± 0.412
0.771AsnCys: 0.771 ± 0.125
3.607AsnAsp: 3.607 ± 0.263
5.534AsnGlu: 5.534 ± 0.432
2.203AsnPhe: 2.203 ± 0.193
3.717AsnGly: 3.717 ± 0.362
0.936AsnHis: 0.936 ± 0.127
4.158AsnIle: 4.158 ± 0.346
6.746AsnLys: 6.746 ± 0.451
4.626AsnLeu: 4.626 ± 0.34
1.046AsnMet: 1.046 ± 0.162
4.103AsnAsn: 4.103 ± 0.373
2.065AsnPro: 2.065 ± 0.285
1.817AsnGln: 1.817 ± 0.235
2.285AsnArg: 2.285 ± 0.24
3.91AsnSer: 3.91 ± 0.398
3.772AsnThr: 3.772 ± 0.421
3.442AsnVal: 3.442 ± 0.309
0.716AsnTrp: 0.716 ± 0.142
2.698AsnTyr: 2.698 ± 0.322
0.0AsnXaa: 0.0 ± 0.0
Pro
1.377ProAla: 1.377 ± 0.232
0.138ProCys: 0.138 ± 0.061
1.955ProAsp: 1.955 ± 0.206
2.423ProGlu: 2.423 ± 0.262
1.267ProPhe: 1.267 ± 0.235
0.854ProGly: 0.854 ± 0.13
0.826ProHis: 0.826 ± 0.147
1.597ProIle: 1.597 ± 0.234
2.148ProLys: 2.148 ± 0.264
2.23ProLeu: 2.23 ± 0.288
0.413ProMet: 0.413 ± 0.098
1.349ProAsn: 1.349 ± 0.218
0.578ProPro: 0.578 ± 0.137
0.798ProGln: 0.798 ± 0.134
0.991ProArg: 0.991 ± 0.147
1.845ProSer: 1.845 ± 0.289
1.349ProThr: 1.349 ± 0.201
1.322ProVal: 1.322 ± 0.215
0.248ProTrp: 0.248 ± 0.086
1.404ProTyr: 1.404 ± 0.191
0.0ProXaa: 0.0 ± 0.0
Gln
2.23GlnAla: 2.23 ± 0.395
0.33GlnCys: 0.33 ± 0.09
1.68GlnAsp: 1.68 ± 0.23
3.056GlnGlu: 3.056 ± 0.457
1.514GlnPhe: 1.514 ± 0.22
1.624GlnGly: 1.624 ± 0.216
0.606GlnHis: 0.606 ± 0.135
2.368GlnIle: 2.368 ± 0.28
3.249GlnLys: 3.249 ± 0.428
4.13GlnLeu: 4.13 ± 0.398
1.046GlnMet: 1.046 ± 0.163
2.258GlnAsn: 2.258 ± 0.296
0.716GlnPro: 0.716 ± 0.138
1.9GlnGln: 1.9 ± 0.511
1.432GlnArg: 1.432 ± 0.183
2.175GlnSer: 2.175 ± 0.262
1.597GlnThr: 1.597 ± 0.231
2.313GlnVal: 2.313 ± 0.242
0.441GlnTrp: 0.441 ± 0.114
1.349GlnTyr: 1.349 ± 0.185
0.0GlnXaa: 0.0 ± 0.0
Arg
1.652ArgAla: 1.652 ± 0.215
0.193ArgCys: 0.193 ± 0.081
2.01ArgAsp: 2.01 ± 0.231
2.588ArgGlu: 2.588 ± 0.241
1.955ArgPhe: 1.955 ± 0.292
2.01ArgGly: 2.01 ± 0.288
0.551ArgHis: 0.551 ± 0.153
3.056ArgIle: 3.056 ± 0.278
3.194ArgLys: 3.194 ± 0.256
3.662ArgLeu: 3.662 ± 0.362
1.239ArgMet: 1.239 ± 0.198
2.836ArgAsn: 2.836 ± 0.29
0.798ArgPro: 0.798 ± 0.162
1.542ArgGln: 1.542 ± 0.2
1.569ArgArg: 1.569 ± 0.252
2.203ArgSer: 2.203 ± 0.269
1.569ArgThr: 1.569 ± 0.263
2.616ArgVal: 2.616 ± 0.26
0.606ArgTrp: 0.606 ± 0.125
1.872ArgTyr: 1.872 ± 0.204
0.0ArgXaa: 0.0 ± 0.0
Ser
3.056SerAla: 3.056 ± 0.4
0.936SerCys: 0.936 ± 0.18
4.736SerAsp: 4.736 ± 0.409
4.846SerGlu: 4.846 ± 0.414
3.579SerPhe: 3.579 ± 0.293
3.387SerGly: 3.387 ± 0.319
1.019SerHis: 1.019 ± 0.177
5.176SerIle: 5.176 ± 0.479
6.25SerLys: 6.25 ± 0.436
5.782SerLeu: 5.782 ± 0.5
1.597SerMet: 1.597 ± 0.224
4.103SerAsn: 4.103 ± 0.329
2.01SerPro: 2.01 ± 0.241
2.258SerGln: 2.258 ± 0.278
2.093SerArg: 2.093 ± 0.237
5.342SerSer: 5.342 ± 0.598
3.882SerThr: 3.882 ± 0.443
4.13SerVal: 4.13 ± 0.311
0.578SerTrp: 0.578 ± 0.131
2.368SerTyr: 2.368 ± 0.215
0.0SerXaa: 0.0 ± 0.0
Thr
3.056ThrAla: 3.056 ± 0.377
0.248ThrCys: 0.248 ± 0.099
3.359ThrAsp: 3.359 ± 0.297
3.937ThrGlu: 3.937 ± 0.341
2.285ThrPhe: 2.285 ± 0.246
3.607ThrGly: 3.607 ± 0.349
0.798ThrHis: 0.798 ± 0.168
4.047ThrIle: 4.047 ± 0.391
5.342ThrLys: 5.342 ± 0.354
4.818ThrLeu: 4.818 ± 0.303
1.129ThrMet: 1.129 ± 0.154
2.808ThrAsn: 2.808 ± 0.283
1.735ThrPro: 1.735 ± 0.196
2.148ThrGln: 2.148 ± 0.258
1.707ThrArg: 1.707 ± 0.217
3.442ThrSer: 3.442 ± 0.403
3.056ThrThr: 3.056 ± 0.371
4.763ThrVal: 4.763 ± 0.371
0.551ThrTrp: 0.551 ± 0.11
3.166ThrTyr: 3.166 ± 0.283
0.0ThrXaa: 0.0 ± 0.0
Val
3.249ValAla: 3.249 ± 0.228
0.468ValCys: 0.468 ± 0.111
3.992ValAsp: 3.992 ± 0.334
4.571ValGlu: 4.571 ± 0.369
2.451ValPhe: 2.451 ± 0.29
3.332ValGly: 3.332 ± 0.354
1.184ValHis: 1.184 ± 0.184
3.965ValIle: 3.965 ± 0.401
5.975ValLys: 5.975 ± 0.45
4.763ValLeu: 4.763 ± 0.439
1.239ValMet: 1.239 ± 0.2
3.359ValAsn: 3.359 ± 0.329
1.735ValPro: 1.735 ± 0.236
2.478ValGln: 2.478 ± 0.293
2.258ValArg: 2.258 ± 0.249
4.846ValSer: 4.846 ± 0.401
3.579ValThr: 3.579 ± 0.342
3.277ValVal: 3.277 ± 0.29
0.551ValTrp: 0.551 ± 0.133
3.221ValTyr: 3.221 ± 0.376
0.0ValXaa: 0.0 ± 0.0
Trp
0.551TrpAla: 0.551 ± 0.112
0.165TrpCys: 0.165 ± 0.07
0.854TrpAsp: 0.854 ± 0.174
0.688TrpGlu: 0.688 ± 0.122
0.606TrpPhe: 0.606 ± 0.126
0.633TrpGly: 0.633 ± 0.126
0.193TrpHis: 0.193 ± 0.071
0.798TrpIle: 0.798 ± 0.141
0.771TrpLys: 0.771 ± 0.134
0.854TrpLeu: 0.854 ± 0.16
0.22TrpMet: 0.22 ± 0.115
0.661TrpAsn: 0.661 ± 0.144
0.0TrpPro: 0.0 ± 0.0
0.303TrpGln: 0.303 ± 0.109
0.578TrpArg: 0.578 ± 0.139
0.551TrpSer: 0.551 ± 0.101
0.606TrpThr: 0.606 ± 0.116
0.633TrpVal: 0.633 ± 0.142
0.11TrpTrp: 0.11 ± 0.056
0.716TrpTyr: 0.716 ± 0.172
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.68TyrAla: 1.68 ± 0.214
0.385TyrCys: 0.385 ± 0.107
3.332TyrAsp: 3.332 ± 0.257
3.827TyrGlu: 3.827 ± 0.361
2.506TyrPhe: 2.506 ± 0.347
2.395TyrGly: 2.395 ± 0.301
0.771TyrHis: 0.771 ± 0.155
3.8TyrIle: 3.8 ± 0.288
4.653TyrLys: 4.653 ± 0.37
4.24TyrLeu: 4.24 ± 0.308
1.046TyrMet: 1.046 ± 0.17
2.258TyrAsn: 2.258 ± 0.219
1.239TyrPro: 1.239 ± 0.165
1.68TyrGln: 1.68 ± 0.176
1.762TyrArg: 1.762 ± 0.241
2.808TyrSer: 2.808 ± 0.313
2.781TyrThr: 2.781 ± 0.266
2.533TyrVal: 2.533 ± 0.238
0.468TyrTrp: 0.468 ± 0.105
2.148TyrTyr: 2.148 ± 0.248
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 175 proteins (36320 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski