Cladophialophora carrionii
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11181 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C1CJS8|A0A1C1CJS8_9EURO Putative oxidoreductase OS=Cladophialophora carrionii OX=86049 GN=CLCR_05151 PE=4 SV=1
MM1 pKa = 7.98 PIPHH5 pKa = 7.4 EE6 pKa = 3.61 IHH8 pKa = 6.81 AAFDD12 pKa = 3.46 QFGDD16 pKa = 3.67 LDD18 pKa = 4.24 FSEE21 pKa = 4.48 LMYY24 pKa = 11.02 GIVLFVNLGRR34 pKa = 11.84 HH35 pKa = 5.17 FCLTRR40 pKa = 11.84 YY41 pKa = 9.53 YY42 pKa = 11.0 AFEE45 pKa = 4.24 NFVLDD50 pKa = 3.32 TCDD53 pKa = 3.42 EE54 pKa = 3.68 IRR56 pKa = 11.84 YY57 pKa = 9.38 RR58 pKa = 11.84 DD59 pKa = 3.88 PSCDD63 pKa = 3.01 RR64 pKa = 11.84 SGATLVGLIVLIVVLSATPGPVALMVVALLLCEE97 pKa = 4.08 MVKK100 pKa = 10.55 LLPEE104 pKa = 3.96 MVMGRR109 pKa = 11.84 EE110 pKa = 3.65 WDD112 pKa = 3.62 YY113 pKa = 11.99 DD114 pKa = 3.83 DD115 pKa = 6.38 DD116 pKa = 4.68 EE117 pKa = 5.71 EE118 pKa = 5.66 GVDD121 pKa = 3.71 YY122 pKa = 10.83 EE123 pKa = 4.26 YY124 pKa = 11.27 DD125 pKa = 3.62 EE126 pKa = 5.05 EE127 pKa = 5.7 GLDD130 pKa = 4.13 FEE132 pKa = 5.78 HH133 pKa = 8.01 DD134 pKa = 3.62 EE135 pKa = 4.48 EE136 pKa = 6.13 GLDD139 pKa = 5.64 FEE141 pKa = 6.4 DD142 pKa = 3.57 EE143 pKa = 4.58 TNVEE147 pKa = 4.12 KK148 pKa = 10.9 RR149 pKa = 3.4
Molecular weight: 17.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.923
IPC_protein 3.897
Toseland 3.706
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.783
Rodwell 3.732
Grimsley 3.617
Solomon 3.872
Lehninger 3.821
Nozaki 3.986
DTASelect 4.177
Thurlkill 3.745
EMBOSS 3.795
Sillero 4.012
Patrickios 1.138
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.905
Protein with the highest isoelectric point:
>tr|A0A1C1C6P1|A0A1C1C6P1_9EURO Putative transporter OS=Cladophialophora carrionii OX=86049 GN=CLCR_00621 PE=4 SV=1
MM1 pKa = 7.54 LRR3 pKa = 11.84 PPQVPAQAQAPASFEE18 pKa = 3.51 GAARR22 pKa = 11.84 VKK24 pKa = 10.56 KK25 pKa = 9.81 PAGARR30 pKa = 11.84 GGMRR34 pKa = 11.84 GGRR37 pKa = 11.84 GAPRR41 pKa = 11.84 GRR43 pKa = 11.84 GAPGRR48 pKa = 11.84 GAPRR52 pKa = 11.84 GRR54 pKa = 11.84 GSFGGGSFGGGRR66 pKa = 11.84 GAPRR70 pKa = 11.84 GGGRR74 pKa = 11.84 GGFSGGRR81 pKa = 11.84 GGGPRR86 pKa = 11.84 GGGFGSRR93 pKa = 11.84 GRR95 pKa = 11.84 GRR97 pKa = 11.84 GG98 pKa = 3.24
Molecular weight: 9.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.504
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.457
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.179
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.168
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11181
0
11181
5263867
57
6597
470.8
52.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.97 ± 0.017
1.154 ± 0.009
5.782 ± 0.016
6.097 ± 0.021
3.591 ± 0.015
6.923 ± 0.022
2.493 ± 0.011
4.607 ± 0.013
4.674 ± 0.018
8.821 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.137 ± 0.009
3.469 ± 0.012
6.238 ± 0.024
4.17 ± 0.017
6.361 ± 0.02
8.152 ± 0.024
6.068 ± 0.019
6.199 ± 0.015
1.431 ± 0.008
2.664 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here