Southern rice black-streaked dwarf virus
Average proteome isoelectric point is 5.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J9UCP0|J9UCP0_9REOV p2 OS=Southern rice black-streaked dwarf virus OX=519497 GN=SRBSDVs2_gp1 PE=4 SV=1
MM1 pKa = 7.72 NYY3 pKa = 10.32 NDD5 pKa = 5.51 ANDD8 pKa = 4.05 SYY10 pKa = 11.47 QLNHH14 pKa = 5.08 VTSLEE19 pKa = 3.9 FDD21 pKa = 3.87 PLDD24 pKa = 3.98 PEE26 pKa = 4.59 VNLISQDD33 pKa = 3.22 FDD35 pKa = 4.49 DD36 pKa = 5.24 YY37 pKa = 11.5 DD38 pKa = 3.9 YY39 pKa = 11.82 HH40 pKa = 8.49 DD41 pKa = 5.37 LEE43 pKa = 4.73 VNSLSEE49 pKa = 4.79 DD50 pKa = 3.57 LSDD53 pKa = 3.8 MNLMATRR60 pKa = 11.84 IKK62 pKa = 10.77 NFPDD66 pKa = 2.94 DD67 pKa = 3.5 TFEE70 pKa = 4.34 VFEE73 pKa = 4.95 SLDD76 pKa = 3.8 APPPLASLIYY86 pKa = 10.57 SEE88 pKa = 6.32 VSDD91 pKa = 4.08 EE92 pKa = 4.08 WCDD95 pKa = 2.99 IDD97 pKa = 4.36 NFLDD101 pKa = 3.27 IRR103 pKa = 11.84 VVNDD107 pKa = 3.08 EE108 pKa = 4.34 SQFEE112 pKa = 4.42 FVNSHH117 pKa = 5.45 INSRR121 pKa = 11.84 LLITLNSNPNILWTAVGLLTKK142 pKa = 10.47 ISLLQEE148 pKa = 4.08 FEE150 pKa = 3.93 NFEE153 pKa = 4.17 ILNYY157 pKa = 7.18 WQAMEE162 pKa = 4.83 RR163 pKa = 11.84 RR164 pKa = 11.84 WDD166 pKa = 4.27 LMNDD170 pKa = 3.34 DD171 pKa = 4.74 LKK173 pKa = 11.08 IGFVLRR179 pKa = 11.84 AFDD182 pKa = 4.37 LKK184 pKa = 11.0 QNQFEE189 pKa = 4.91 LLTKK193 pKa = 10.61 LLRR196 pKa = 11.84 DD197 pKa = 3.55 SLFFAGINLIGKK209 pKa = 9.7 SSMLPMLTVHH219 pKa = 6.98 SISDD223 pKa = 4.46 YY224 pKa = 9.45 IDD226 pKa = 2.61 HH227 pKa = 7.3 WFPTEE232 pKa = 4.78 CYY234 pKa = 10.47 SSDD237 pKa = 2.91 NFMSFIKK244 pKa = 10.34 CHH246 pKa = 6.66 TITVPKK252 pKa = 8.31 WKK254 pKa = 10.21 KK255 pKa = 10.09 IVVQFYY261 pKa = 10.22 LRR263 pKa = 11.84 QIFSRR268 pKa = 11.84 SRR270 pKa = 11.84 TQVLMAHH277 pKa = 6.41 VDD279 pKa = 2.99 IDD281 pKa = 2.87 HH282 pKa = 7.02 WYY284 pKa = 9.94 DD285 pKa = 3.23 VFMKK289 pKa = 9.96 TLVFKK294 pKa = 10.74 SMRR297 pKa = 11.84 KK298 pKa = 6.31 TKK300 pKa = 10.69 KK301 pKa = 8.69 MLKK304 pKa = 10.36 NILNLL309 pKa = 3.61
Molecular weight: 36.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.616
IPC2_protein 4.596
IPC_protein 4.571
Toseland 4.406
ProMoST 4.711
Dawson 4.546
Bjellqvist 4.698
Wikipedia 4.457
Rodwell 4.418
Grimsley 4.317
Solomon 4.546
Lehninger 4.507
Nozaki 4.66
DTASelect 4.889
Thurlkill 4.418
EMBOSS 4.482
Sillero 4.698
Patrickios 3.872
IPC_peptide 4.546
IPC2_peptide 4.685
IPC2.peptide.svr19 4.643
Protein with the highest isoelectric point:
>tr|J9UL77|J9UL77_9REOV p6 OS=Southern rice black-streaked dwarf virus OX=519497 GN=SRBSDVs6_gp1 PE=4 SV=1
MM1 pKa = 7.44 VEE3 pKa = 4.25 RR4 pKa = 11.84 KK5 pKa = 9.21 FSRR8 pKa = 11.84 SAEE11 pKa = 3.79 ICLKK15 pKa = 10.4 KK16 pKa = 10.67 FNSIQKK22 pKa = 9.36 FRR24 pKa = 11.84 QNKK27 pKa = 6.82 QQQKK31 pKa = 6.49 TNRR34 pKa = 11.84 DD35 pKa = 3.04 RR36 pKa = 11.84 TISQKK41 pKa = 8.86 EE42 pKa = 3.94 KK43 pKa = 10.23 KK44 pKa = 9.87 IKK46 pKa = 10.43 DD47 pKa = 3.56 RR48 pKa = 11.84 APPPSKK54 pKa = 10.72 KK55 pKa = 10.05 EE56 pKa = 4.22 DD57 pKa = 3.39 IPTVSTPISIEE68 pKa = 4.04 SQSNLDD74 pKa = 3.75 EE75 pKa = 4.06 QRR77 pKa = 11.84 TSYY80 pKa = 10.48 IHH82 pKa = 7.04 LLHH85 pKa = 6.41 QKK87 pKa = 10.85 DD88 pKa = 4.33 SILDD92 pKa = 4.16 AIIEE96 pKa = 4.16 DD97 pKa = 3.94 AEE99 pKa = 4.26 NIRR102 pKa = 11.84 NVFIGEE108 pKa = 4.09 VPLTKK113 pKa = 10.33 EE114 pKa = 3.79 IIKK117 pKa = 10.53 NRR119 pKa = 11.84 LDD121 pKa = 4.99 DD122 pKa = 4.1 ISTVLNNASDD132 pKa = 5.27 LILKK136 pKa = 9.99 LFEE139 pKa = 4.8 SDD141 pKa = 3.58 NGILNEE147 pKa = 4.23 CVIPNDD153 pKa = 3.83 VIQKK157 pKa = 9.9 DD158 pKa = 3.87 IYY160 pKa = 10.82 SILHH164 pKa = 5.84 PSEE167 pKa = 3.91 IFDD170 pKa = 3.89 YY171 pKa = 11.19 NGFGIDD177 pKa = 3.78 EE178 pKa = 4.45 VNNLAISPKK187 pKa = 9.73 IRR189 pKa = 11.84 YY190 pKa = 8.16 LPNWNIQKK198 pKa = 9.54 IRR200 pKa = 11.84 STIDD204 pKa = 3.03 LLKK207 pKa = 10.75 NDD209 pKa = 4.32 NSPLKK214 pKa = 9.71 PVKK217 pKa = 9.87 LAHH220 pKa = 7.19 DD221 pKa = 4.01 YY222 pKa = 11.7 GLISNNLKK230 pKa = 10.2 RR231 pKa = 11.84 KK232 pKa = 9.74 LLIGVDD238 pKa = 3.19 RR239 pKa = 11.84 MFNKK243 pKa = 10.54 LSIQHH248 pKa = 6.56 RR249 pKa = 11.84 FGTFEE254 pKa = 4.02 SQIFSCFLMKK264 pKa = 10.43 QLLTEE269 pKa = 4.12 EE270 pKa = 4.04 QCYY273 pKa = 10.95 VNLPNCVLKK282 pKa = 11.07 SKK284 pKa = 9.34 FQKK287 pKa = 10.46 RR288 pKa = 11.84 GIDD291 pKa = 3.71 TVCWQYY297 pKa = 11.39 LRR299 pKa = 11.84 QIILHH304 pKa = 5.39 YY305 pKa = 10.81 NGFPFYY311 pKa = 10.53 HH312 pKa = 6.35 HH313 pKa = 5.68 THH315 pKa = 5.83 SARR318 pKa = 11.84 FGVLDD323 pKa = 3.42 STKK326 pKa = 10.42 ISIHH330 pKa = 5.93 TDD332 pKa = 3.31 LPVSCVLPSVLHH344 pKa = 6.04 GLLNGIVSEE353 pKa = 4.47 LLCDD357 pKa = 4.42 LNSEE361 pKa = 4.27 TALIYY366 pKa = 10.49 AKK368 pKa = 10.48 HH369 pKa = 6.63 LMFCSQQSTYY379 pKa = 9.5 QRR381 pKa = 11.84 QLDD384 pKa = 3.99 FKK386 pKa = 11.06 DD387 pKa = 4.56 SFRR390 pKa = 11.84 SWPKK394 pKa = 10.12 LCYY397 pKa = 10.17 DD398 pKa = 3.87 SIGYY402 pKa = 8.77 VLAVLYY408 pKa = 10.85 SSDD411 pKa = 3.86 MKK413 pKa = 10.77 PLEE416 pKa = 3.78 QRR418 pKa = 11.84 IEE420 pKa = 4.51 HH421 pKa = 6.81 IDD423 pKa = 3.38 SEE425 pKa = 4.52 FWMDD429 pKa = 3.47 EE430 pKa = 3.92 KK431 pKa = 10.96 EE432 pKa = 4.32 KK433 pKa = 10.77 EE434 pKa = 3.98 ILEE437 pKa = 4.43 TSVSQLPDD445 pKa = 3.37 YY446 pKa = 10.75 VKK448 pKa = 10.83 KK449 pKa = 10.54 FVSSSIAHH457 pKa = 5.91 CTDD460 pKa = 3.27 LGNIVKK466 pKa = 10.36 SRR468 pKa = 11.84 MALNNNISLSRR479 pKa = 11.84 EE480 pKa = 4.01 LTNDD484 pKa = 2.58 EE485 pKa = 4.27 VTSVRR490 pKa = 11.84 LLASKK495 pKa = 8.86 NTEE498 pKa = 3.75 FLRR501 pKa = 11.84 WLTMTSTIISNFGIYY516 pKa = 10.61 NKK518 pKa = 9.99 TSLLLEE524 pKa = 4.27 YY525 pKa = 9.65 EE526 pKa = 4.21 KK527 pKa = 11.17 SVGTKK532 pKa = 9.64 QDD534 pKa = 3.29 VMVSKK539 pKa = 9.74 PEE541 pKa = 4.0 CKK543 pKa = 9.65 ISIVTEE549 pKa = 3.59 QGMNIPLINDD559 pKa = 3.46 WSKK562 pKa = 11.2 VPILNEE568 pKa = 3.56 MSEE571 pKa = 5.5 LFDD574 pKa = 4.53 QNWKK578 pKa = 10.36 SDD580 pKa = 3.96 LIKK583 pKa = 10.59 EE584 pKa = 4.3 FEE586 pKa = 5.34 HH587 pKa = 8.44 IDD589 pKa = 3.58 DD590 pKa = 3.89 FEE592 pKa = 5.02 KK593 pKa = 10.95 RR594 pKa = 11.84 FVTFLTNKK602 pKa = 10.33 SGGQKK607 pKa = 9.91 SEE609 pKa = 3.98 EE610 pKa = 3.99 PTLSKK615 pKa = 10.27 EE616 pKa = 4.29 LKK618 pKa = 9.58 GISNARR624 pKa = 11.84 VIAFALNRR632 pKa = 11.84 NDD634 pKa = 3.39 YY635 pKa = 10.71 HH636 pKa = 8.54 DD637 pKa = 3.89 EE638 pKa = 4.02 LKK640 pKa = 10.49 FIKK643 pKa = 9.93 MLMAYY648 pKa = 7.37 GKK650 pKa = 10.16 CAIRR654 pKa = 11.84 FQIDD658 pKa = 2.4 RR659 pKa = 11.84 RR660 pKa = 11.84 ARR662 pKa = 11.84 VIVIVPNAIQSSEE675 pKa = 4.12 LFLLLGFNVLKK686 pKa = 10.14 TNKK689 pKa = 9.3 KK690 pKa = 9.53 HH691 pKa = 5.81 NDD693 pKa = 3.23 KK694 pKa = 10.79 IAVGKK699 pKa = 10.45 QIGNLLDD706 pKa = 4.98 ARR708 pKa = 11.84 VQMCNTGDD716 pKa = 3.4 VSSVKK721 pKa = 10.56 NSGDD725 pKa = 3.54 MKK727 pKa = 11.29 GMDD730 pKa = 3.31 AHH732 pKa = 7.02 TIPNLTLFLRR742 pKa = 11.84 NKK744 pKa = 8.94 MIEE747 pKa = 4.08 VLFEE751 pKa = 4.91 LDD753 pKa = 3.49 PSKK756 pKa = 11.1 SCSNFFFSEE765 pKa = 4.11 DD766 pKa = 3.05 KK767 pKa = 10.84 EE768 pKa = 4.32 YY769 pKa = 10.25 TLKK772 pKa = 10.76 EE773 pKa = 3.75 KK774 pKa = 10.58 HH775 pKa = 6.49 PKK777 pKa = 9.94 FEE779 pKa = 4.81 EE780 pKa = 4.13 IYY782 pKa = 10.05 NRR784 pKa = 11.84 NLRR787 pKa = 11.84 GVVIHH792 pKa = 6.77 AAKK795 pKa = 10.41 CLFYY799 pKa = 9.79 MFSMNMYY806 pKa = 10.64 LEE808 pKa = 4.3 DD809 pKa = 4.05 HH810 pKa = 6.72 FFADD814 pKa = 3.72 SQTVSDD820 pKa = 3.68 QTFQTGFFATSAQHH834 pKa = 6.21 TLFLSLFLLNLEE846 pKa = 4.3 RR847 pKa = 11.84 KK848 pKa = 9.59 FFAKK852 pKa = 10.25 LDD854 pKa = 3.63 NKK856 pKa = 9.46 MISVMHH862 pKa = 6.11 SVMGDD867 pKa = 3.4 DD868 pKa = 3.43 VLEE871 pKa = 4.45 VIKK874 pKa = 11.13 NGVKK878 pKa = 10.48 FPDD881 pKa = 4.05 KK882 pKa = 10.52 VRR884 pKa = 11.84 SWLSLRR890 pKa = 11.84 KK891 pKa = 9.51 NDD893 pKa = 4.3 LAKK896 pKa = 10.83 LNYY899 pKa = 9.79 EE900 pKa = 4.27 EE901 pKa = 4.67 EE902 pKa = 4.52 LSLSRR907 pKa = 11.84 MYY909 pKa = 11.18 GVFLQQAALLGVYY922 pKa = 9.48 VPYY925 pKa = 9.82 PSRR928 pKa = 11.84 MSLFCDD934 pKa = 3.27 EE935 pKa = 5.69 RR936 pKa = 11.84 SDD938 pKa = 3.42 TTKK941 pKa = 10.84 RR942 pKa = 11.84 HH943 pKa = 5.04 TLDD946 pKa = 3.21 MIKK949 pKa = 10.13 IVMDD953 pKa = 4.35 VISAKK958 pKa = 9.84 AQRR961 pKa = 11.84 SYY963 pKa = 11.62 GIDD966 pKa = 3.09 NGLGIGYY973 pKa = 8.44 SIWSCHH979 pKa = 5.23 RR980 pKa = 11.84 SSRR983 pKa = 11.84 YY984 pKa = 9.32 IYY986 pKa = 10.97 SDD988 pKa = 3.23 FDD990 pKa = 3.73 KK991 pKa = 11.46 SKK993 pKa = 10.7 VSQLLEE999 pKa = 3.67 MDD1001 pKa = 3.37 KK1002 pKa = 10.82 TFDD1005 pKa = 3.46 FNFIVSHH1012 pKa = 7.29 DD1013 pKa = 3.88 EE1014 pKa = 4.09 TEE1016 pKa = 3.82 KK1017 pKa = 10.73 SIRR1020 pKa = 11.84 FIYY1023 pKa = 9.99 PFVTVMCSPISWPMLTFVMNDD1044 pKa = 2.4 ISDD1047 pKa = 3.84 PNKK1050 pKa = 10.27 FITYY1054 pKa = 9.27 KK1055 pKa = 10.64 SKK1057 pKa = 11.19 AFTSLNGDD1065 pKa = 3.66 GAYY1068 pKa = 9.87 MLINQMFFTSNEE1080 pKa = 3.36 QHH1082 pKa = 5.79 MFQFVDD1088 pKa = 3.91 YY1089 pKa = 10.22 VKK1091 pKa = 9.77 HH1092 pKa = 4.8 QSKK1095 pKa = 10.24 IVEE1098 pKa = 4.33 LKK1100 pKa = 10.91 LNSNFLNWEE1109 pKa = 4.07 ARR1111 pKa = 11.84 HH1112 pKa = 5.48 HH1113 pKa = 5.89 WGFTFGEE1120 pKa = 3.93 HH1121 pKa = 6.87 LLRR1124 pKa = 11.84 FKK1126 pKa = 10.84 RR1127 pKa = 11.84 LRR1129 pKa = 11.84 HH1130 pKa = 4.77 LTEE1133 pKa = 3.64 SRR1135 pKa = 11.84 KK1136 pKa = 10.1 NEE1138 pKa = 4.1 LGLGDD1143 pKa = 4.04 IEE1145 pKa = 5.34 VMVHH1149 pKa = 6.29 NLNKK1153 pKa = 10.4 YY1154 pKa = 9.73 LDD1156 pKa = 3.98 GNRR1159 pKa = 11.84 VLLSFNSISVLKK1171 pKa = 9.74 AHH1173 pKa = 7.08 KK1174 pKa = 10.3 IEE1176 pKa = 4.08 VPYY1179 pKa = 11.24 GLMYY1183 pKa = 10.97 ANHH1186 pKa = 6.26 NRR1188 pKa = 11.84 SKK1190 pKa = 10.27 IDD1192 pKa = 3.0 QSLTVRR1198 pKa = 11.84 TEE1200 pKa = 3.93 SLEE1203 pKa = 3.73 EE1204 pKa = 3.64 RR1205 pKa = 11.84 VYY1207 pKa = 11.27 LDD1209 pKa = 4.57 SIFLKK1214 pKa = 10.93 HH1215 pKa = 5.9 MLDD1218 pKa = 3.82 YY1219 pKa = 10.03 KK1220 pKa = 9.81 TVPKK1224 pKa = 10.48 DD1225 pKa = 3.48 LEE1227 pKa = 4.25 VLKK1230 pKa = 9.34 THH1232 pKa = 7.02 ALCSVRR1238 pKa = 11.84 LRR1240 pKa = 11.84 IYY1242 pKa = 11.02 GDD1244 pKa = 3.34 FDD1246 pKa = 3.98 YY1247 pKa = 11.26 ACEE1250 pKa = 3.98 KK1251 pKa = 10.97 DD1252 pKa = 4.49 GVIEE1256 pKa = 4.32 PSSDD1260 pKa = 2.99 AQFNIILPFLPGYY1273 pKa = 9.6 HH1274 pKa = 6.68 LNSAYY1279 pKa = 10.4 GKK1281 pKa = 9.88 LFLYY1285 pKa = 9.0 STLPTVHH1292 pKa = 7.12 DD1293 pKa = 4.13 RR1294 pKa = 11.84 DD1295 pKa = 3.41 ISGTLGEE1302 pKa = 4.42 ITGSLGASFDD1312 pKa = 3.11 VDD1314 pKa = 3.3 AAIEE1318 pKa = 4.19 FGAHH1322 pKa = 5.06 VFNINPSLVDD1332 pKa = 3.39 AAGTAIGIPQGLLNHH1347 pKa = 6.22 YY1348 pKa = 9.49 KK1349 pKa = 10.41 NLVNAFVTNNFNLKK1363 pKa = 9.11 YY1364 pKa = 10.43 HH1365 pKa = 6.84 SIFLNVKK1372 pKa = 8.86 YY1373 pKa = 10.56 FGLNGSLKK1381 pKa = 10.29 HH1382 pKa = 6.07 FSTFGDD1388 pKa = 3.68 YY1389 pKa = 10.71 SSRR1392 pKa = 11.84 LVRR1395 pKa = 11.84 FDD1397 pKa = 5.79 LIHH1400 pKa = 7.23 KK1401 pKa = 9.66 LDD1403 pKa = 4.03 RR1404 pKa = 11.84 YY1405 pKa = 10.3 HH1406 pKa = 6.84 NVFVRR1411 pKa = 11.84 DD1412 pKa = 4.6 FIFAYY1417 pKa = 9.42 IHH1419 pKa = 5.98 EE1420 pKa = 4.78 LNGRR1424 pKa = 11.84 RR1425 pKa = 11.84 VYY1427 pKa = 11.07 LDD1429 pKa = 3.58 YY1430 pKa = 11.25 SLHH1433 pKa = 6.15 SLLLVMSRR1441 pKa = 11.84 GSIKK1445 pKa = 10.67 YY1446 pKa = 7.16 FTSHH1450 pKa = 7.3 LSQLFNPMLTLEE1462 pKa = 4.52 FDD1464 pKa = 3.42
Molecular weight: 168.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.873
IPC2_protein 7.249
IPC_protein 7.102
Toseland 7.044
ProMoST 7.673
Dawson 7.79
Bjellqvist 8.112
Wikipedia 7.732
Rodwell 7.819
Grimsley 7.176
Solomon 7.819
Lehninger 7.834
Nozaki 8.287
DTASelect 7.966
Thurlkill 8.024
EMBOSS 8.039
Sillero 8.273
Patrickios 4.355
IPC_peptide 7.819
IPC2_peptide 7.22
IPC2.peptide.svr19 7.219
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
9107
209
1464
758.9
87.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.964 ± 0.269
1.482 ± 0.136
6.248 ± 0.195
5.776 ± 0.331
6.358 ± 0.319
3.997 ± 0.255
2.569 ± 0.177
7.05 ± 0.243
6.665 ± 0.243
10.355 ± 0.392
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.262 ± 0.136
6.995 ± 0.293
3.25 ± 0.162
3.118 ± 0.108
4.085 ± 0.161
8.773 ± 0.514
5.897 ± 0.463
6.127 ± 0.161
0.747 ± 0.086
4.282 ± 0.346
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here