Lake Sarah-associated circular molecule 3

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126G8F9|A0A126G8F9_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular molecule 3 OX=1685728 PE=3 SV=1
MM1 pKa = 6.48YY2 pKa = 7.91TNPNSYY8 pKa = 10.8FDD10 pKa = 3.42LTLPQRR16 pKa = 11.84RR17 pKa = 11.84FMFTSYY23 pKa = 9.73TLEE26 pKa = 4.56GEE28 pKa = 4.55GFTVPDD34 pKa = 3.05QDD36 pKa = 3.14PRR38 pKa = 11.84FRR40 pKa = 11.84YY41 pKa = 9.24CVYY44 pKa = 10.28QAEE47 pKa = 4.74KK48 pKa = 10.57CPQTGNIHH56 pKa = 5.34LQGYY60 pKa = 9.55IEE62 pKa = 5.08FKK64 pKa = 10.85DD65 pKa = 3.6QIRR68 pKa = 11.84GNTVQKK74 pKa = 10.82LMNVPGLHH82 pKa = 7.05LSRR85 pKa = 11.84VDD87 pKa = 3.63YY88 pKa = 10.49PEE90 pKa = 4.2KK91 pKa = 10.77ARR93 pKa = 11.84AYY95 pKa = 10.46CMKK98 pKa = 10.35LDD100 pKa = 3.52TRR102 pKa = 11.84YY103 pKa = 10.54AEE105 pKa = 3.98PQEE108 pKa = 3.75YY109 pKa = 10.47GKK111 pKa = 10.72YY112 pKa = 9.98KK113 pKa = 10.5KK114 pKa = 10.2EE115 pKa = 3.52QGARR119 pKa = 11.84NDD121 pKa = 3.77LKK123 pKa = 11.69AMGDD127 pKa = 3.59MVLAGATHH135 pKa = 5.77IQIYY139 pKa = 9.81DD140 pKa = 4.18AYY142 pKa = 8.14PGQYY146 pKa = 8.81IRR148 pKa = 11.84YY149 pKa = 8.44YY150 pKa = 10.51KK151 pKa = 10.71AIDD154 pKa = 3.64RR155 pKa = 11.84LRR157 pKa = 11.84QIYY160 pKa = 10.61ALDD163 pKa = 3.47KK164 pKa = 10.68PNRR167 pKa = 11.84LKK169 pKa = 10.41IGRR172 pKa = 11.84MEE174 pKa = 5.23CILFYY179 pKa = 10.97GRR181 pKa = 11.84PGTGKK186 pKa = 6.92TTQAKK191 pKa = 9.66EE192 pKa = 3.72YY193 pKa = 10.07AAHH196 pKa = 7.11IKK198 pKa = 8.5QAYY201 pKa = 7.45WEE203 pKa = 4.18IPIANSGTLWFDD215 pKa = 3.78GYY217 pKa = 10.98NAEE220 pKa = 4.25PCVIIDD226 pKa = 4.52DD227 pKa = 4.36FSGKK231 pKa = 10.02INLDD235 pKa = 2.99AALRR239 pKa = 11.84IFDD242 pKa = 3.59QGEE245 pKa = 3.94VQVPVKK251 pKa = 10.43GGHH254 pKa = 5.41VWWCPKK260 pKa = 10.03TIIITSNVHH269 pKa = 5.33PSNWYY274 pKa = 9.93DD275 pKa = 3.42YY276 pKa = 10.68QNRR279 pKa = 11.84QDD281 pKa = 4.75SMIAMYY287 pKa = 10.29RR288 pKa = 11.84RR289 pKa = 11.84LHH291 pKa = 7.05RR292 pKa = 11.84IVCFNRR298 pKa = 11.84ATPAEE303 pKa = 3.91VDD305 pKa = 3.14RR306 pKa = 11.84FKK308 pKa = 11.3FLDD311 pKa = 3.46IPEE314 pKa = 4.33NAFDD318 pKa = 5.2VIMEE322 pKa = 4.51TDD324 pKa = 2.97QQ325 pKa = 4.18

Molecular weight: 37.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126G8F9|A0A126G8F9_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular molecule 3 OX=1685728 PE=3 SV=1
MM1 pKa = 6.48YY2 pKa = 7.91TNPNSYY8 pKa = 10.8FDD10 pKa = 3.42LTLPQRR16 pKa = 11.84RR17 pKa = 11.84FMFTSYY23 pKa = 9.73TLEE26 pKa = 4.56GEE28 pKa = 4.55GFTVPDD34 pKa = 3.05QDD36 pKa = 3.14PRR38 pKa = 11.84FRR40 pKa = 11.84YY41 pKa = 9.24CVYY44 pKa = 10.28QAEE47 pKa = 4.74KK48 pKa = 10.57CPQTGNIHH56 pKa = 5.34LQGYY60 pKa = 9.55IEE62 pKa = 5.08FKK64 pKa = 10.85DD65 pKa = 3.6QIRR68 pKa = 11.84GNTVQKK74 pKa = 10.82LMNVPGLHH82 pKa = 7.05LSRR85 pKa = 11.84VDD87 pKa = 3.63YY88 pKa = 10.49PEE90 pKa = 4.2KK91 pKa = 10.77ARR93 pKa = 11.84AYY95 pKa = 10.46CMKK98 pKa = 10.35LDD100 pKa = 3.52TRR102 pKa = 11.84YY103 pKa = 10.54AEE105 pKa = 3.98PQEE108 pKa = 3.75YY109 pKa = 10.47GKK111 pKa = 10.72YY112 pKa = 9.98KK113 pKa = 10.5KK114 pKa = 10.2EE115 pKa = 3.52QGARR119 pKa = 11.84NDD121 pKa = 3.77LKK123 pKa = 11.69AMGDD127 pKa = 3.59MVLAGATHH135 pKa = 5.77IQIYY139 pKa = 9.81DD140 pKa = 4.18AYY142 pKa = 8.14PGQYY146 pKa = 8.81IRR148 pKa = 11.84YY149 pKa = 8.44YY150 pKa = 10.51KK151 pKa = 10.71AIDD154 pKa = 3.64RR155 pKa = 11.84LRR157 pKa = 11.84QIYY160 pKa = 10.61ALDD163 pKa = 3.47KK164 pKa = 10.68PNRR167 pKa = 11.84LKK169 pKa = 10.41IGRR172 pKa = 11.84MEE174 pKa = 5.23CILFYY179 pKa = 10.97GRR181 pKa = 11.84PGTGKK186 pKa = 6.92TTQAKK191 pKa = 9.66EE192 pKa = 3.72YY193 pKa = 10.07AAHH196 pKa = 7.11IKK198 pKa = 8.5QAYY201 pKa = 7.45WEE203 pKa = 4.18IPIANSGTLWFDD215 pKa = 3.78GYY217 pKa = 10.98NAEE220 pKa = 4.25PCVIIDD226 pKa = 4.52DD227 pKa = 4.36FSGKK231 pKa = 10.02INLDD235 pKa = 2.99AALRR239 pKa = 11.84IFDD242 pKa = 3.59QGEE245 pKa = 3.94VQVPVKK251 pKa = 10.43GGHH254 pKa = 5.41VWWCPKK260 pKa = 10.03TIIITSNVHH269 pKa = 5.33PSNWYY274 pKa = 9.93DD275 pKa = 3.42YY276 pKa = 10.68QNRR279 pKa = 11.84QDD281 pKa = 4.75SMIAMYY287 pKa = 10.29RR288 pKa = 11.84RR289 pKa = 11.84LHH291 pKa = 7.05RR292 pKa = 11.84IVCFNRR298 pKa = 11.84ATPAEE303 pKa = 3.91VDD305 pKa = 3.14RR306 pKa = 11.84FKK308 pKa = 11.3FLDD311 pKa = 3.46IPEE314 pKa = 4.33NAFDD318 pKa = 5.2VIMEE322 pKa = 4.51TDD324 pKa = 2.97QQ325 pKa = 4.18

Molecular weight: 37.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

325

325

325

325.0

37.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.077 ± 0.0

2.154 ± 0.0

6.769 ± 0.0

4.923 ± 0.0

4.308 ± 0.0

6.462 ± 0.0

2.154 ± 0.0

7.385 ± 0.0

5.846 ± 0.0

5.846 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.077 ± 0.0

4.615 ± 0.0

5.538 ± 0.0

5.846 ± 0.0

6.769 ± 0.0

2.462 ± 0.0

5.231 ± 0.0

4.615 ± 0.0

1.538 ± 0.0

7.385 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski