Escherichia phage LL5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tempevirinae; Tlsvirus; Escherichia virus LL5

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4Q2Q4|A0A2Z4Q2Q4_9CAUD Uncharacterized protein OS=Escherichia phage LL5 OX=2233992 GN=CPT_LL5_28 PE=4 SV=1
MM1 pKa = 7.18QFEE4 pKa = 4.72LTRR7 pKa = 11.84VTVEE11 pKa = 3.63ILNDD15 pKa = 3.58GTYY18 pKa = 11.12DD19 pKa = 3.68GMKK22 pKa = 10.21HH23 pKa = 4.7LTFPIRR29 pKa = 11.84IEE31 pKa = 3.86NCLDD35 pKa = 3.27YY36 pKa = 10.95GLEE39 pKa = 3.97VGMVDD44 pKa = 3.69VPLDD48 pKa = 3.57ALVAAGYY55 pKa = 10.53DD56 pKa = 3.58KK57 pKa = 11.49VKK59 pKa = 11.09GGMNPEE65 pKa = 4.74SPDD68 pKa = 3.82CMDD71 pKa = 4.52SDD73 pKa = 4.12TFLPFSYY80 pKa = 10.65GEE82 pKa = 4.09CRR84 pKa = 11.84IVDD87 pKa = 3.48

Molecular weight: 9.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q396|A0A2Z4Q396_9CAUD Uncharacterized protein OS=Escherichia phage LL5 OX=2233992 GN=CPT_LL5_37 PE=4 SV=1
MM1 pKa = 7.77TYY3 pKa = 10.56FKK5 pKa = 10.66NIKK8 pKa = 8.02TGRR11 pKa = 11.84IYY13 pKa = 10.69RR14 pKa = 11.84VNLRR18 pKa = 11.84HH19 pKa = 6.27KK20 pKa = 10.3NALIMSKK27 pKa = 9.84SGCWVRR33 pKa = 11.84SARR36 pKa = 11.84YY37 pKa = 9.47RR38 pKa = 11.84NADD41 pKa = 3.14LTGYY45 pKa = 9.67PFIAVNSTNSS55 pKa = 3.03

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

15312

35

1258

174.0

19.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.137 ± 0.384

1.365 ± 0.17

6.328 ± 0.238

6.955 ± 0.276

3.997 ± 0.179

7.021 ± 0.26

1.822 ± 0.211

6.772 ± 0.209

7.51 ± 0.372

6.831 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.213 ± 0.188

5.003 ± 0.246

3.226 ± 0.213

3.788 ± 0.327

4.807 ± 0.199

5.682 ± 0.215

5.368 ± 0.284

6.877 ± 0.236

1.417 ± 0.108

3.879 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski