Streptococcus satellite phage Javan760
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZX61|A0A4D5ZX61_9VIRU PriCT_1 domain-containing protein OS=Streptococcus satellite phage Javan760 OX=2558856 GN=JavanS760_0014 PE=4 SV=1
MM1 pKa = 7.87 EE2 pKa = 4.51 EE3 pKa = 3.56 TTYY6 pKa = 11.03 DD7 pKa = 3.82 IIAKK11 pKa = 9.74 SLDD14 pKa = 4.0 RR15 pKa = 11.84 ISMEE19 pKa = 3.74 LHH21 pKa = 5.91 QADD24 pKa = 4.33 EE25 pKa = 4.59 NNDD28 pKa = 3.14 FLEE31 pKa = 4.3 YY32 pKa = 10.95 GFYY35 pKa = 10.31 QDD37 pKa = 3.87 NN38 pKa = 3.58
Molecular weight: 4.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.91
IPC_protein 3.757
Toseland 3.592
ProMoST 3.948
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A4D5ZX55|A0A4D5ZX55_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan760 OX=2558856 GN=JavanS760_0004 PE=4 SV=1
MM1 pKa = 7.46 EE2 pKa = 4.55 KK3 pKa = 10.02 WEE5 pKa = 4.17 NQDD8 pKa = 5.67 KK9 pKa = 10.99 ILLDD13 pKa = 3.44 KK14 pKa = 11.03 NKK16 pKa = 10.2 RR17 pKa = 11.84 GKK19 pKa = 9.84 DD20 pKa = 3.38 RR21 pKa = 11.84 NWRR24 pKa = 11.84 GRR26 pKa = 11.84 KK27 pKa = 8.96 LLSLKK32 pKa = 10.48 LADD35 pKa = 3.45 IFKK38 pKa = 9.97 EE39 pKa = 3.64 LGYY42 pKa = 10.4 RR43 pKa = 11.84 EE44 pKa = 4.04 TLIEE48 pKa = 4.0 RR49 pKa = 11.84 VEE51 pKa = 4.38 TCGDD55 pKa = 3.29 TLRR58 pKa = 11.84 FIRR61 pKa = 11.84 RR62 pKa = 11.84 EE63 pKa = 4.07 DD64 pKa = 3.35 GSLRR68 pKa = 11.84 LYY70 pKa = 9.72 QAYY73 pKa = 9.5 FCKK76 pKa = 10.73 NKK78 pKa = 9.96 LCPMCNWRR86 pKa = 11.84 RR87 pKa = 11.84 SMKK90 pKa = 10.24 YY91 pKa = 9.92 SYY93 pKa = 8.93 QTSQIVDD100 pKa = 3.57 EE101 pKa = 5.37 AIKK104 pKa = 9.44 EE105 pKa = 4.12 QPKK108 pKa = 10.58 GRR110 pKa = 11.84 FLFLCC115 pKa = 4.63
Molecular weight: 13.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.13
IPC2_protein 8.873
IPC_protein 8.829
Toseland 9.604
ProMoST 9.326
Dawson 9.838
Bjellqvist 9.589
Wikipedia 9.984
Rodwell 10.189
Grimsley 9.882
Solomon 9.882
Lehninger 9.853
Nozaki 9.794
DTASelect 9.516
Thurlkill 9.706
EMBOSS 10.028
Sillero 9.809
Patrickios 7.6
IPC_peptide 9.882
IPC2_peptide 8.39
IPC2.peptide.svr19 7.904
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
27
0
27
3476
38
484
128.7
14.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.006 ± 0.322
0.633 ± 0.169
6.214 ± 0.446
8.832 ± 0.667
4.114 ± 0.389
4.603 ± 0.333
1.266 ± 0.276
7.221 ± 0.456
9.609 ± 0.536
10.587 ± 0.419
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.848 ± 0.239
5.092 ± 0.366
2.934 ± 0.355
3.625 ± 0.241
5.639 ± 0.285
5.667 ± 0.331
5.524 ± 0.549
5.437 ± 0.33
0.978 ± 0.224
4.171 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here