Amino acid dipepetide frequency for Escherichia phage Henu8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.653AlaAla: 6.653 ± 0.977
0.731AlaCys: 0.731 ± 0.257
4.094AlaAsp: 4.094 ± 0.68
4.46AlaGlu: 4.46 ± 0.615
2.924AlaPhe: 2.924 ± 0.496
4.533AlaGly: 4.533 ± 0.581
1.097AlaHis: 1.097 ± 0.241
5.629AlaIle: 5.629 ± 0.791
7.53AlaLys: 7.53 ± 1.043
6.58AlaLeu: 6.58 ± 0.661
2.924AlaMet: 2.924 ± 0.377
3.875AlaAsn: 3.875 ± 0.71
1.682AlaPro: 1.682 ± 0.295
2.998AlaGln: 2.998 ± 0.565
4.46AlaArg: 4.46 ± 0.624
5.703AlaSer: 5.703 ± 0.871
4.094AlaThr: 4.094 ± 0.856
5.703AlaVal: 5.703 ± 0.793
1.097AlaTrp: 1.097 ± 0.263
2.851AlaTyr: 2.851 ± 0.37
0.0AlaXaa: 0.0 ± 0.0
Cys
1.17CysAla: 1.17 ± 0.297
0.073CysCys: 0.073 ± 0.067
1.682CysAsp: 1.682 ± 0.345
0.95CysGlu: 0.95 ± 0.263
0.292CysPhe: 0.292 ± 0.157
1.608CysGly: 1.608 ± 0.454
0.219CysHis: 0.219 ± 0.132
0.512CysIle: 0.512 ± 0.189
1.097CysLys: 1.097 ± 0.399
0.804CysLeu: 0.804 ± 0.227
0.512CysMet: 0.512 ± 0.165
0.658CysAsn: 0.658 ± 0.228
0.366CysPro: 0.366 ± 0.166
0.219CysGln: 0.219 ± 0.12
0.95CysArg: 0.95 ± 0.337
0.439CysSer: 0.439 ± 0.178
0.804CysThr: 0.804 ± 0.25
1.024CysVal: 1.024 ± 0.228
0.439CysTrp: 0.439 ± 0.192
0.366CysTyr: 0.366 ± 0.147
0.0CysXaa: 0.0 ± 0.0
Asp
5.337AspAla: 5.337 ± 0.646
0.439AspCys: 0.439 ± 0.186
3.948AspAsp: 3.948 ± 0.523
4.313AspGlu: 4.313 ± 0.522
2.924AspPhe: 2.924 ± 0.368
6.214AspGly: 6.214 ± 0.807
0.877AspHis: 0.877 ± 0.265
3.509AspIle: 3.509 ± 0.511
3.656AspLys: 3.656 ± 0.456
4.387AspLeu: 4.387 ± 0.507
1.682AspMet: 1.682 ± 0.348
2.778AspAsn: 2.778 ± 0.466
2.047AspPro: 2.047 ± 0.358
1.828AspGln: 1.828 ± 0.381
2.998AspArg: 2.998 ± 0.462
3.509AspSer: 3.509 ± 0.476
3.29AspThr: 3.29 ± 0.429
3.582AspVal: 3.582 ± 0.469
1.097AspTrp: 1.097 ± 0.31
2.34AspTyr: 2.34 ± 0.37
0.0AspXaa: 0.0 ± 0.0
Glu
5.849GluAla: 5.849 ± 0.704
1.17GluCys: 1.17 ± 0.342
3.217GluAsp: 3.217 ± 0.389
4.24GluGlu: 4.24 ± 0.614
3.436GluPhe: 3.436 ± 0.576
3.436GluGly: 3.436 ± 0.505
1.17GluHis: 1.17 ± 0.275
6.141GluIle: 6.141 ± 0.585
4.094GluLys: 4.094 ± 0.678
5.045GluLeu: 5.045 ± 0.686
2.778GluMet: 2.778 ± 0.441
3.729GluAsn: 3.729 ± 0.549
1.682GluPro: 1.682 ± 0.384
2.924GluGln: 2.924 ± 0.552
3.071GluArg: 3.071 ± 0.541
4.606GluSer: 4.606 ± 0.532
3.656GluThr: 3.656 ± 0.516
4.46GluVal: 4.46 ± 0.543
0.731GluTrp: 0.731 ± 0.262
2.998GluTyr: 2.998 ± 0.404
0.0GluXaa: 0.0 ± 0.0
Phe
2.193PheAla: 2.193 ± 0.296
0.512PheCys: 0.512 ± 0.188
3.071PheAsp: 3.071 ± 0.458
2.559PheGlu: 2.559 ± 0.475
1.024PhePhe: 1.024 ± 0.325
2.998PheGly: 2.998 ± 0.47
0.512PheHis: 0.512 ± 0.204
2.047PheIle: 2.047 ± 0.322
2.924PheLys: 2.924 ± 0.387
2.193PheLeu: 2.193 ± 0.524
1.243PheMet: 1.243 ± 0.337
2.34PheAsn: 2.34 ± 0.424
1.389PhePro: 1.389 ± 0.351
1.389PheGln: 1.389 ± 0.304
2.413PheArg: 2.413 ± 0.439
1.901PheSer: 1.901 ± 0.367
3.071PheThr: 3.071 ± 0.457
2.851PheVal: 2.851 ± 0.424
0.658PheTrp: 0.658 ± 0.224
1.097PheTyr: 1.097 ± 0.359
0.0PheXaa: 0.0 ± 0.0
Gly
4.46GlyAla: 4.46 ± 0.699
1.389GlyCys: 1.389 ± 0.322
5.045GlyAsp: 5.045 ± 0.735
5.41GlyGlu: 5.41 ± 0.647
3.363GlyPhe: 3.363 ± 0.466
5.776GlyGly: 5.776 ± 1.037
1.462GlyHis: 1.462 ± 0.326
3.509GlyIle: 3.509 ± 0.497
6.58GlyLys: 6.58 ± 0.51
4.606GlyLeu: 4.606 ± 0.511
2.705GlyMet: 2.705 ± 0.391
3.729GlyAsn: 3.729 ± 0.497
0.512GlyPro: 0.512 ± 0.231
2.047GlyGln: 2.047 ± 0.341
2.705GlyArg: 2.705 ± 0.38
5.191GlySer: 5.191 ± 0.514
3.656GlyThr: 3.656 ± 0.641
6.434GlyVal: 6.434 ± 0.675
1.389GlyTrp: 1.389 ± 0.287
3.144GlyTyr: 3.144 ± 0.43
0.0GlyXaa: 0.0 ± 0.0
His
0.512HisAla: 0.512 ± 0.234
0.366HisCys: 0.366 ± 0.215
0.95HisAsp: 0.95 ± 0.272
0.877HisGlu: 0.877 ± 0.308
0.804HisPhe: 0.804 ± 0.23
1.389HisGly: 1.389 ± 0.313
0.439HisHis: 0.439 ± 0.206
1.389HisIle: 1.389 ± 0.349
1.535HisLys: 1.535 ± 0.309
1.097HisLeu: 1.097 ± 0.276
0.439HisMet: 0.439 ± 0.202
0.804HisAsn: 0.804 ± 0.315
0.658HisPro: 0.658 ± 0.239
0.658HisGln: 0.658 ± 0.277
1.17HisArg: 1.17 ± 0.349
0.731HisSer: 0.731 ± 0.218
0.877HisThr: 0.877 ± 0.255
1.535HisVal: 1.535 ± 0.303
0.292HisTrp: 0.292 ± 0.132
1.389HisTyr: 1.389 ± 0.351
0.0HisXaa: 0.0 ± 0.0
Ile
6.287IleAla: 6.287 ± 0.702
1.097IleCys: 1.097 ± 0.328
4.752IleAsp: 4.752 ± 0.511
4.971IleGlu: 4.971 ± 0.438
2.047IlePhe: 2.047 ± 0.376
4.46IleGly: 4.46 ± 0.522
1.462IleHis: 1.462 ± 0.388
3.875IleIle: 3.875 ± 0.518
5.264IleLys: 5.264 ± 0.635
2.632IleLeu: 2.632 ± 0.35
1.828IleMet: 1.828 ± 0.472
2.924IleAsn: 2.924 ± 0.528
2.193IlePro: 2.193 ± 0.358
2.705IleGln: 2.705 ± 0.559
2.998IleArg: 2.998 ± 0.46
4.094IleSer: 4.094 ± 0.541
4.46IleThr: 4.46 ± 0.496
3.656IleVal: 3.656 ± 0.453
1.097IleTrp: 1.097 ± 0.338
2.266IleTyr: 2.266 ± 0.335
0.0IleXaa: 0.0 ± 0.0
Lys
7.019LysAla: 7.019 ± 1.244
0.95LysCys: 0.95 ± 0.292
4.606LysAsp: 4.606 ± 0.563
5.776LysGlu: 5.776 ± 0.661
2.998LysPhe: 2.998 ± 0.439
4.021LysGly: 4.021 ± 0.423
1.389LysHis: 1.389 ± 0.408
3.948LysIle: 3.948 ± 0.528
4.46LysLys: 4.46 ± 0.704
5.41LysLeu: 5.41 ± 0.574
3.656LysMet: 3.656 ± 0.482
3.363LysAsn: 3.363 ± 0.826
3.656LysPro: 3.656 ± 0.561
3.582LysGln: 3.582 ± 0.579
3.802LysArg: 3.802 ± 0.558
3.802LysSer: 3.802 ± 0.503
4.752LysThr: 4.752 ± 0.555
5.045LysVal: 5.045 ± 0.596
1.024LysTrp: 1.024 ± 0.276
1.974LysTyr: 1.974 ± 0.401
0.0LysXaa: 0.0 ± 0.0
Leu
5.556LeuAla: 5.556 ± 0.676
0.658LeuCys: 0.658 ± 0.214
3.217LeuAsp: 3.217 ± 0.514
4.387LeuGlu: 4.387 ± 0.702
1.682LeuPhe: 1.682 ± 0.335
3.509LeuGly: 3.509 ± 0.701
1.389LeuHis: 1.389 ± 0.302
4.825LeuIle: 4.825 ± 0.644
5.045LeuLys: 5.045 ± 0.664
3.875LeuLeu: 3.875 ± 0.458
1.608LeuMet: 1.608 ± 0.311
3.875LeuAsn: 3.875 ± 0.524
3.217LeuPro: 3.217 ± 0.492
1.828LeuGln: 1.828 ± 0.399
3.802LeuArg: 3.802 ± 0.495
4.387LeuSer: 4.387 ± 0.461
4.167LeuThr: 4.167 ± 0.523
3.802LeuVal: 3.802 ± 0.457
1.17LeuTrp: 1.17 ± 0.301
2.193LeuTyr: 2.193 ± 0.396
0.0LeuXaa: 0.0 ± 0.0
Met
3.509MetAla: 3.509 ± 0.461
0.366MetCys: 0.366 ± 0.141
1.243MetAsp: 1.243 ± 0.278
1.828MetGlu: 1.828 ± 0.443
0.877MetPhe: 0.877 ± 0.305
0.877MetGly: 0.877 ± 0.219
0.804MetHis: 0.804 ± 0.217
2.632MetIle: 2.632 ± 0.453
2.632MetLys: 2.632 ± 0.442
2.266MetLeu: 2.266 ± 0.418
1.024MetMet: 1.024 ± 0.316
1.389MetAsn: 1.389 ± 0.345
1.17MetPro: 1.17 ± 0.289
1.462MetGln: 1.462 ± 0.303
1.682MetArg: 1.682 ± 0.31
2.486MetSer: 2.486 ± 0.432
1.682MetThr: 1.682 ± 0.341
1.828MetVal: 1.828 ± 0.381
0.292MetTrp: 0.292 ± 0.151
1.097MetTyr: 1.097 ± 0.274
0.0MetXaa: 0.0 ± 0.0
Asn
4.606AsnAla: 4.606 ± 0.799
0.658AsnCys: 0.658 ± 0.241
3.071AsnAsp: 3.071 ± 0.473
3.436AsnGlu: 3.436 ± 0.436
1.828AsnPhe: 1.828 ± 0.303
6.141AsnGly: 6.141 ± 0.867
0.877AsnHis: 0.877 ± 0.256
3.071AsnIle: 3.071 ± 0.435
3.436AsnLys: 3.436 ± 0.534
2.193AsnLeu: 2.193 ± 0.328
1.097AsnMet: 1.097 ± 0.325
2.851AsnAsn: 2.851 ± 0.484
1.974AsnPro: 1.974 ± 0.304
2.047AsnGln: 2.047 ± 0.469
1.901AsnArg: 1.901 ± 0.309
2.851AsnSer: 2.851 ± 0.73
1.316AsnThr: 1.316 ± 0.26
3.144AsnVal: 3.144 ± 0.417
0.585AsnTrp: 0.585 ± 0.165
1.682AsnTyr: 1.682 ± 0.38
0.0AsnXaa: 0.0 ± 0.0
Pro
2.266ProAla: 2.266 ± 0.463
0.658ProCys: 0.658 ± 0.263
2.266ProAsp: 2.266 ± 0.452
3.217ProGlu: 3.217 ± 0.695
1.462ProPhe: 1.462 ± 0.306
3.071ProGly: 3.071 ± 0.56
0.439ProHis: 0.439 ± 0.176
1.974ProIle: 1.974 ± 0.423
1.608ProLys: 1.608 ± 0.333
1.608ProLeu: 1.608 ± 0.288
1.024ProMet: 1.024 ± 0.285
1.608ProAsn: 1.608 ± 0.331
1.243ProPro: 1.243 ± 0.408
0.95ProGln: 0.95 ± 0.301
1.828ProArg: 1.828 ± 0.385
1.901ProSer: 1.901 ± 0.335
1.316ProThr: 1.316 ± 0.257
2.851ProVal: 2.851 ± 0.541
0.512ProTrp: 0.512 ± 0.189
1.682ProTyr: 1.682 ± 0.315
0.0ProXaa: 0.0 ± 0.0
Gln
3.217GlnAla: 3.217 ± 0.516
0.731GlnCys: 0.731 ± 0.203
1.608GlnAsp: 1.608 ± 0.31
2.266GlnGlu: 2.266 ± 0.338
1.316GlnPhe: 1.316 ± 0.284
2.266GlnGly: 2.266 ± 0.31
0.95GlnHis: 0.95 ± 0.286
2.632GlnIle: 2.632 ± 0.489
2.193GlnLys: 2.193 ± 0.457
2.705GlnLeu: 2.705 ± 0.568
0.95GlnMet: 0.95 ± 0.356
1.389GlnAsn: 1.389 ± 0.311
1.682GlnPro: 1.682 ± 0.495
2.559GlnGln: 2.559 ± 0.822
2.047GlnArg: 2.047 ± 0.413
2.413GlnSer: 2.413 ± 0.318
1.682GlnThr: 1.682 ± 0.402
3.363GlnVal: 3.363 ± 0.465
0.512GlnTrp: 0.512 ± 0.195
1.974GlnTyr: 1.974 ± 0.339
0.0GlnXaa: 0.0 ± 0.0
Arg
3.509ArgAla: 3.509 ± 0.477
0.658ArgCys: 0.658 ± 0.271
2.851ArgAsp: 2.851 ± 0.449
3.217ArgGlu: 3.217 ± 0.398
1.682ArgPhe: 1.682 ± 0.345
3.144ArgGly: 3.144 ± 0.385
0.585ArgHis: 0.585 ± 0.244
4.167ArgIle: 4.167 ± 0.407
4.167ArgLys: 4.167 ± 0.547
2.998ArgLeu: 2.998 ± 0.501
1.828ArgMet: 1.828 ± 0.38
2.12ArgAsn: 2.12 ± 0.476
1.828ArgPro: 1.828 ± 0.397
2.193ArgGln: 2.193 ± 0.531
3.656ArgArg: 3.656 ± 0.595
3.071ArgSer: 3.071 ± 0.473
1.755ArgThr: 1.755 ± 0.339
4.971ArgVal: 4.971 ± 0.707
0.658ArgTrp: 0.658 ± 0.201
2.266ArgTyr: 2.266 ± 0.414
0.0ArgXaa: 0.0 ± 0.0
Ser
5.118SerAla: 5.118 ± 0.76
0.731SerCys: 0.731 ± 0.212
3.802SerAsp: 3.802 ± 0.419
5.337SerGlu: 5.337 ± 0.612
2.924SerPhe: 2.924 ± 0.454
5.629SerGly: 5.629 ± 0.629
1.024SerHis: 1.024 ± 0.248
4.752SerIle: 4.752 ± 0.543
4.971SerLys: 4.971 ± 0.684
4.24SerLeu: 4.24 ± 0.428
1.097SerMet: 1.097 ± 0.238
2.34SerAsn: 2.34 ± 0.487
1.682SerPro: 1.682 ± 0.374
2.12SerGln: 2.12 ± 0.404
3.217SerArg: 3.217 ± 0.501
4.021SerSer: 4.021 ± 0.776
3.144SerThr: 3.144 ± 0.504
4.606SerVal: 4.606 ± 0.594
1.097SerTrp: 1.097 ± 0.321
1.901SerTyr: 1.901 ± 0.365
0.0SerXaa: 0.0 ± 0.0
Thr
4.167ThrAla: 4.167 ± 0.786
0.804ThrCys: 0.804 ± 0.345
2.851ThrAsp: 2.851 ± 0.388
3.217ThrGlu: 3.217 ± 0.576
2.266ThrPhe: 2.266 ± 0.37
5.922ThrGly: 5.922 ± 0.745
0.292ThrHis: 0.292 ± 0.138
2.998ThrIle: 2.998 ± 0.478
3.802ThrLys: 3.802 ± 0.756
3.875ThrLeu: 3.875 ± 0.429
1.316ThrMet: 1.316 ± 0.319
3.582ThrAsn: 3.582 ± 0.485
2.486ThrPro: 2.486 ± 0.414
2.34ThrGln: 2.34 ± 0.315
1.901ThrArg: 1.901 ± 0.306
4.24ThrSer: 4.24 ± 0.537
2.486ThrThr: 2.486 ± 0.437
3.802ThrVal: 3.802 ± 0.455
0.585ThrTrp: 0.585 ± 0.173
1.828ThrTyr: 1.828 ± 0.376
0.0ThrXaa: 0.0 ± 0.0
Val
4.971ValAla: 4.971 ± 0.734
1.243ValCys: 1.243 ± 0.298
5.118ValAsp: 5.118 ± 0.463
4.825ValGlu: 4.825 ± 0.585
2.705ValPhe: 2.705 ± 0.504
4.021ValGly: 4.021 ± 0.548
1.462ValHis: 1.462 ± 0.382
4.825ValIle: 4.825 ± 0.542
6.799ValLys: 6.799 ± 0.734
4.679ValLeu: 4.679 ± 0.468
2.047ValMet: 2.047 ± 0.309
3.071ValAsn: 3.071 ± 0.451
2.12ValPro: 2.12 ± 0.558
2.34ValGln: 2.34 ± 0.502
2.705ValArg: 2.705 ± 0.439
4.898ValSer: 4.898 ± 0.686
4.825ValThr: 4.825 ± 0.547
5.118ValVal: 5.118 ± 0.501
0.804ValTrp: 0.804 ± 0.189
1.755ValTyr: 1.755 ± 0.272
0.0ValXaa: 0.0 ± 0.0
Trp
0.658TrpAla: 0.658 ± 0.202
0.366TrpCys: 0.366 ± 0.207
1.024TrpAsp: 1.024 ± 0.208
0.658TrpGlu: 0.658 ± 0.214
0.804TrpPhe: 0.804 ± 0.28
1.389TrpGly: 1.389 ± 0.311
0.658TrpHis: 0.658 ± 0.209
0.804TrpIle: 0.804 ± 0.232
0.877TrpLys: 0.877 ± 0.233
0.877TrpLeu: 0.877 ± 0.234
0.366TrpMet: 0.366 ± 0.153
0.658TrpAsn: 0.658 ± 0.246
0.366TrpPro: 0.366 ± 0.193
0.366TrpGln: 0.366 ± 0.178
1.389TrpArg: 1.389 ± 0.294
0.95TrpSer: 0.95 ± 0.23
0.95TrpThr: 0.95 ± 0.248
0.585TrpVal: 0.585 ± 0.196
0.146TrpTrp: 0.146 ± 0.113
0.585TrpTyr: 0.585 ± 0.182
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.559TyrAla: 2.559 ± 0.526
0.585TyrCys: 0.585 ± 0.19
2.34TyrAsp: 2.34 ± 0.384
2.486TyrGlu: 2.486 ± 0.433
1.024TyrPhe: 1.024 ± 0.239
2.778TyrGly: 2.778 ± 0.414
0.804TyrHis: 0.804 ± 0.229
1.828TyrIle: 1.828 ± 0.291
2.559TyrLys: 2.559 ± 0.415
2.12TyrLeu: 2.12 ± 0.406
0.95TyrMet: 0.95 ± 0.274
1.682TyrAsn: 1.682 ± 0.283
1.535TyrPro: 1.535 ± 0.324
1.901TyrGln: 1.901 ± 0.269
2.632TyrArg: 2.632 ± 0.462
2.559TyrSer: 2.559 ± 0.505
2.778TyrThr: 2.778 ± 0.342
1.974TyrVal: 1.974 ± 0.319
0.292TyrTrp: 0.292 ± 0.133
1.243TyrTyr: 1.243 ± 0.286
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (13679 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski