Amino acid dipepetide frequency for Novosphingobium lindaniclasticum LE124

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.62AlaAla: 18.62 ± 0.202	1.155AlaCys: 1.155 ± 0.032	7.115AlaAsp: 7.115 ± 0.079	7.986AlaGlu: 7.986 ± 0.094	4.344AlaPhe: 4.344 ± 0.057	11.131AlaGly: 11.131 ± 0.117	2.317AlaHis: 2.317 ± 0.045	6.348AlaIle: 6.348 ± 0.073	4.014AlaLys: 4.014 ± 0.071	13.932AlaLeu: 13.932 ± 0.129	3.908AlaMet: 3.908 ± 0.059	3.009AlaAsn: 3.009 ± 0.051	6.337AlaPro: 6.337 ± 0.081	4.674AlaGln: 4.674 ± 0.061	9.827AlaArg: 9.827 ± 0.104	6.902AlaSer: 6.902 ± 0.081	5.924AlaThr: 5.924 ± 0.067	8.578AlaVal: 8.578 ± 0.09	1.745AlaTrp: 1.745 ± 0.041	2.613AlaTyr: 2.613 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	1.02CysAla: 1.02 ± 0.031	0.089CysCys: 0.089 ± 0.008	0.526CysAsp: 0.526 ± 0.018	0.471CysGlu: 0.471 ± 0.019	0.283CysPhe: 0.283 ± 0.014	0.894CysGly: 0.894 ± 0.029	0.227CysHis: 0.227 ± 0.015	0.347CysIle: 0.347 ± 0.015	0.191CysLys: 0.191 ± 0.012	0.764CysLeu: 0.764 ± 0.024	0.17CysMet: 0.17 ± 0.012	0.222CysAsn: 0.222 ± 0.013	0.45CysPro: 0.45 ± 0.02	0.214CysGln: 0.214 ± 0.014	0.61CysArg: 0.61 ± 0.023	0.474CysSer: 0.474 ± 0.018	0.392CysThr: 0.392 ± 0.019	0.54CysVal: 0.54 ± 0.023	0.128CysTrp: 0.128 ± 0.009	0.164CysTyr: 0.164 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.108AspAla: 7.108 ± 0.074	0.48AspCys: 0.48 ± 0.019	3.07AspAsp: 3.07 ± 0.056	3.379AspGlu: 3.379 ± 0.049	2.172AspPhe: 2.172 ± 0.039	5.344AspGly: 5.344 ± 0.079	1.31AspHis: 1.31 ± 0.033	2.772AspIle: 2.772 ± 0.046	1.753AspLys: 1.753 ± 0.041	6.006AspLeu: 6.006 ± 0.073	1.398AspMet: 1.398 ± 0.033	1.363AspAsn: 1.363 ± 0.032	3.641AspPro: 3.641 ± 0.058	1.743AspGln: 1.743 ± 0.031	4.749AspArg: 4.749 ± 0.068	2.357AspSer: 2.357 ± 0.039	2.504AspThr: 2.504 ± 0.048	4.043AspVal: 4.043 ± 0.059	1.133AspTrp: 1.133 ± 0.032	1.602AspTyr: 1.602 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	8.199GluAla: 8.199 ± 0.091	0.369GluCys: 0.369 ± 0.018	3.089GluAsp: 3.089 ± 0.047	3.233GluGlu: 3.233 ± 0.056	1.59GluPhe: 1.59 ± 0.035	4.836GluGly: 4.836 ± 0.061	1.228GluHis: 1.228 ± 0.031	3.035GluIle: 3.035 ± 0.047	2.066GluLys: 2.066 ± 0.046	5.184GluLeu: 5.184 ± 0.075	1.489GluMet: 1.489 ± 0.036	1.551GluAsn: 1.551 ± 0.035	2.599GluPro: 2.599 ± 0.047	2.27GluGln: 2.27 ± 0.049	5.071GluArg: 5.071 ± 0.069	2.438GluSer: 2.438 ± 0.046	3.273GluThr: 3.273 ± 0.052	3.889GluVal: 3.889 ± 0.059	0.841GluTrp: 0.841 ± 0.024	1.002GluTyr: 1.002 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	4.831PheAla: 4.831 ± 0.063	0.337PheCys: 0.337 ± 0.017	2.599PheAsp: 2.599 ± 0.044	2.03PheGlu: 2.03 ± 0.035	1.283PhePhe: 1.283 ± 0.035	3.59PheGly: 3.59 ± 0.055	0.722PheHis: 0.722 ± 0.024	1.336PheIle: 1.336 ± 0.029	0.911PheLys: 0.911 ± 0.025	3.164PheLeu: 3.164 ± 0.049	0.757PheMet: 0.757 ± 0.022	1.001PheAsn: 1.001 ± 0.031	1.477PhePro: 1.477 ± 0.03	0.904PheGln: 0.904 ± 0.026	2.216PheArg: 2.216 ± 0.05	2.182PheSer: 2.182 ± 0.048	2.048PheThr: 2.048 ± 0.042	2.646PheVal: 2.646 ± 0.05	0.521PheTrp: 0.521 ± 0.022	0.867PheTyr: 0.867 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	9.91GlyAla: 9.91 ± 0.104	0.822GlyCys: 0.822 ± 0.023	4.708GlyAsp: 4.708 ± 0.069	5.134GlyGlu: 5.134 ± 0.063	3.501GlyPhe: 3.501 ± 0.044	7.895GlyGly: 7.895 ± 0.105	1.848GlyHis: 1.848 ± 0.04	4.329GlyIle: 4.329 ± 0.053	3.57GlyLys: 3.57 ± 0.048	8.932GlyLeu: 8.932 ± 0.094	2.365GlyMet: 2.365 ± 0.036	2.429GlyAsn: 2.429 ± 0.058	3.579GlyPro: 3.579 ± 0.051	3.041GlyGln: 3.041 ± 0.049	6.469GlyArg: 6.469 ± 0.074	5.142GlySer: 5.142 ± 0.076	4.941GlyThr: 4.941 ± 0.095	6.006GlyVal: 6.006 ± 0.071	1.524GlyTrp: 1.524 ± 0.037	2.392GlyTyr: 2.392 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.472HisAla: 2.472 ± 0.047	0.205HisCys: 0.205 ± 0.013	1.234HisAsp: 1.234 ± 0.036	1.11HisGlu: 1.11 ± 0.029	0.85HisPhe: 0.85 ± 0.027	2.008HisGly: 2.008 ± 0.04	0.569HisHis: 0.569 ± 0.022	0.858HisIle: 0.858 ± 0.026	0.489HisLys: 0.489 ± 0.021	1.95HisLeu: 1.95 ± 0.041	0.469HisMet: 0.469 ± 0.018	0.419HisAsn: 0.419 ± 0.016	1.281HisPro: 1.281 ± 0.032	0.545HisGln: 0.545 ± 0.018	1.507HisArg: 1.507 ± 0.035	0.93HisSer: 0.93 ± 0.027	0.744HisThr: 0.744 ± 0.024	1.581HisVal: 1.581 ± 0.035	0.336HisTrp: 0.336 ± 0.014	0.567HisTyr: 0.567 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.442IleAla: 7.442 ± 0.076	0.436IleCys: 0.436 ± 0.017	3.657IleAsp: 3.657 ± 0.057	3.457IleGlu: 3.457 ± 0.052	1.388IlePhe: 1.388 ± 0.033	4.87IleGly: 4.87 ± 0.059	0.883IleHis: 0.883 ± 0.027	1.593IleIle: 1.593 ± 0.038	1.19IleLys: 1.19 ± 0.031	3.777IleLeu: 3.777 ± 0.058	0.892IleMet: 0.892 ± 0.028	1.257IleAsn: 1.257 ± 0.029	2.227IlePro: 2.227 ± 0.038	1.124IleGln: 1.124 ± 0.032	3.108IleArg: 3.108 ± 0.051	2.687IleSer: 2.687 ± 0.052	2.339IleThr: 2.339 ± 0.043	3.936IleVal: 3.936 ± 0.054	0.587IleTrp: 0.587 ± 0.02	1.017IleTyr: 1.017 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.36LysAla: 4.36 ± 0.071	0.155LysCys: 0.155 ± 0.011	1.613LysAsp: 1.613 ± 0.044	1.316LysGlu: 1.316 ± 0.034	0.874LysPhe: 0.874 ± 0.026	2.777LysGly: 2.777 ± 0.051	0.536LysHis: 0.536 ± 0.021	1.449LysIle: 1.449 ± 0.032	1.068LysLys: 1.068 ± 0.035	3.18LysLeu: 3.18 ± 0.049	0.765LysMet: 0.765 ± 0.026	0.733LysAsn: 0.733 ± 0.026	2.037LysPro: 2.037 ± 0.045	0.963LysGln: 0.963 ± 0.03	2.216LysArg: 2.216 ± 0.05	1.628LysSer: 1.628 ± 0.038	1.746LysThr: 1.746 ± 0.038	2.384LysVal: 2.384 ± 0.043	0.391LysTrp: 0.391 ± 0.015	0.582LysTyr: 0.582 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	14.329LeuAla: 14.329 ± 0.142	0.842LeuCys: 0.842 ± 0.026	6.261LeuAsp: 6.261 ± 0.076	5.285LeuGlu: 5.285 ± 0.076	3.539LeuPhe: 3.539 ± 0.048	8.716LeuGly: 8.716 ± 0.084	1.912LeuHis: 1.912 ± 0.041	4.367LeuIle: 4.367 ± 0.066	3.049LeuLys: 3.049 ± 0.045	9.563LeuLeu: 9.563 ± 0.113	2.077LeuMet: 2.077 ± 0.041	2.296LeuAsn: 2.296 ± 0.043	5.719LeuPro: 5.719 ± 0.06	2.686LeuGln: 2.686 ± 0.045	7.212LeuArg: 7.212 ± 0.089	6.294LeuSer: 6.294 ± 0.071	5.521LeuThr: 5.521 ± 0.06	7.509LeuVal: 7.509 ± 0.076	1.183LeuTrp: 1.183 ± 0.034	1.961LeuTyr: 1.961 ± 0.038	0.0LeuXaa: 0.0 ± 0.0
Met	3.309MetAla: 3.309 ± 0.055	0.145MetCys: 0.145 ± 0.011	1.169MetAsp: 1.169 ± 0.032	1.154MetGlu: 1.154 ± 0.028	0.685MetPhe: 0.685 ± 0.023	1.889MetGly: 1.889 ± 0.041	0.423MetHis: 0.423 ± 0.019	1.348MetIle: 1.348 ± 0.032	0.94MetLys: 0.94 ± 0.027	2.605MetLeu: 2.605 ± 0.045	0.664MetMet: 0.664 ± 0.02	0.715MetAsn: 0.715 ± 0.023	1.467MetPro: 1.467 ± 0.029	0.723MetGln: 0.723 ± 0.024	1.775MetArg: 1.775 ± 0.038	1.487MetSer: 1.487 ± 0.033	1.776MetThr: 1.776 ± 0.037	1.71MetVal: 1.71 ± 0.033	0.185MetTrp: 0.185 ± 0.011	0.248MetTyr: 0.248 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.205AsnAla: 3.205 ± 0.057	0.233AsnCys: 0.233 ± 0.013	1.458AsnAsp: 1.458 ± 0.035	1.116AsnGlu: 1.116 ± 0.033	0.933AsnPhe: 0.933 ± 0.028	2.451AsnGly: 2.451 ± 0.053	0.467AsnHis: 0.467 ± 0.019	1.149AsnIle: 1.149 ± 0.032	0.651AsnLys: 0.651 ± 0.024	2.646AsnLeu: 2.646 ± 0.046	0.551AsnMet: 0.551 ± 0.018	0.656AsnAsn: 0.656 ± 0.026	1.751AsnPro: 1.751 ± 0.035	0.744AsnGln: 0.744 ± 0.026	1.869AsnArg: 1.869 ± 0.038	1.269AsnSer: 1.269 ± 0.036	1.184AsnThr: 1.184 ± 0.029	1.885AsnVal: 1.885 ± 0.041	0.427AsnTrp: 0.427 ± 0.019	0.682AsnTyr: 0.682 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	6.976ProAla: 6.976 ± 0.082	0.377ProCys: 0.377 ± 0.017	3.507ProAsp: 3.507 ± 0.053	3.815ProGlu: 3.815 ± 0.051	1.907ProPhe: 1.907 ± 0.04	4.812ProGly: 4.812 ± 0.062	1.004ProHis: 1.004 ± 0.03	2.316ProIle: 2.316 ± 0.039	1.5ProLys: 1.5 ± 0.033	5.067ProLeu: 5.067 ± 0.056	1.245ProMet: 1.245 ± 0.031	1.187ProAsn: 1.187 ± 0.029	2.495ProPro: 2.495 ± 0.06	1.803ProGln: 1.803 ± 0.042	3.124ProArg: 3.124 ± 0.057	2.935ProSer: 2.935 ± 0.048	2.375ProThr: 2.375 ± 0.049	4.24ProVal: 4.24 ± 0.055	0.722ProTrp: 0.722 ± 0.024	1.037ProTyr: 1.037 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	4.411GlnAla: 4.411 ± 0.074	0.237GlnCys: 0.237 ± 0.015	1.665GlnAsp: 1.665 ± 0.036	1.478GlnGlu: 1.478 ± 0.034	1.127GlnPhe: 1.127 ± 0.03	2.707GlnGly: 2.707 ± 0.043	0.62GlnHis: 0.62 ± 0.02	1.746GlnIle: 1.746 ± 0.04	0.958GlnLys: 0.958 ± 0.027	3.059GlnLeu: 3.059 ± 0.048	0.851GlnMet: 0.851 ± 0.026	0.825GlnAsn: 0.825 ± 0.026	1.821GlnPro: 1.821 ± 0.038	1.228GlnGln: 1.228 ± 0.031	2.452GlnArg: 2.452 ± 0.046	1.697GlnSer: 1.697 ± 0.036	1.672GlnThr: 1.672 ± 0.038	2.474GlnVal: 2.474 ± 0.038	0.476GlnTrp: 0.476 ± 0.018	0.614GlnTyr: 0.614 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	8.305ArgAla: 8.305 ± 0.109	0.5ArgCys: 0.5 ± 0.021	4.171ArgAsp: 4.171 ± 0.057	4.466ArgGlu: 4.466 ± 0.077	3.181ArgPhe: 3.181 ± 0.046	5.13ArgGly: 5.13 ± 0.066	1.753ArgHis: 1.753 ± 0.037	4.201ArgIle: 4.201 ± 0.056	2.447ArgLys: 2.447 ± 0.043	8.185ArgLeu: 8.185 ± 0.098	1.999ArgMet: 1.999 ± 0.04	1.895ArgAsn: 1.895 ± 0.037	3.604ArgPro: 3.604 ± 0.056	2.663ArgGln: 2.663 ± 0.045	6.107ArgArg: 6.107 ± 0.095	4.036ArgSer: 4.036 ± 0.06	3.501ArgThr: 3.501 ± 0.058	4.798ArgVal: 4.798 ± 0.064	1.207ArgTrp: 1.207 ± 0.034	1.935ArgTyr: 1.935 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	6.777SerAla: 6.777 ± 0.084	0.453SerCys: 0.453 ± 0.02	3.101SerAsp: 3.101 ± 0.049	2.97SerGlu: 2.97 ± 0.052	2.118SerPhe: 2.118 ± 0.039	5.746SerGly: 5.746 ± 0.086	1.074SerHis: 1.074 ± 0.032	2.61SerIle: 2.61 ± 0.044	1.521SerLys: 1.521 ± 0.037	5.517SerLeu: 5.517 ± 0.069	1.246SerMet: 1.246 ± 0.029	1.393SerAsn: 1.393 ± 0.035	2.908SerPro: 2.908 ± 0.047	1.762SerGln: 1.762 ± 0.036	3.925SerArg: 3.925 ± 0.057	3.005SerSer: 3.005 ± 0.058	2.682SerThr: 2.682 ± 0.054	3.733SerVal: 3.733 ± 0.05	0.755SerTrp: 0.755 ± 0.024	1.402SerTyr: 1.402 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	6.168ThrAla: 6.168 ± 0.08	0.4ThrCys: 0.4 ± 0.018	2.675ThrAsp: 2.675 ± 0.05	2.47ThrGlu: 2.47 ± 0.042	1.917ThrPhe: 1.917 ± 0.036	5.247ThrGly: 5.247 ± 0.064	0.937ThrHis: 0.937 ± 0.03	2.795ThrIle: 2.795 ± 0.052	1.236ThrLys: 1.236 ± 0.034	5.615ThrLeu: 5.615 ± 0.062	1.208ThrMet: 1.208 ± 0.028	1.245ThrAsn: 1.245 ± 0.033	3.217ThrPro: 3.217 ± 0.052	1.503ThrGln: 1.503 ± 0.034	3.536ThrArg: 3.536 ± 0.05	2.779ThrSer: 2.779 ± 0.058	2.6ThrThr: 2.6 ± 0.054	4.126ThrVal: 4.126 ± 0.076	0.677ThrTrp: 0.677 ± 0.024	1.253ThrTyr: 1.253 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	9.055ValAla: 9.055 ± 0.08	0.59ValCys: 0.59 ± 0.023	4.085ValAsp: 4.085 ± 0.062	4.547ValGlu: 4.547 ± 0.056	2.297ValPhe: 2.297 ± 0.047	5.344ValGly: 5.344 ± 0.064	1.418ValHis: 1.418 ± 0.033	3.746ValIle: 3.746 ± 0.057	2.107ValLys: 2.107 ± 0.045	7.235ValLeu: 7.235 ± 0.085	1.621ValMet: 1.621 ± 0.036	2.02ValAsn: 2.02 ± 0.044	4.052ValPro: 4.052 ± 0.053	2.127ValGln: 2.127 ± 0.04	5.046ValArg: 5.046 ± 0.062	4.352ValSer: 4.352 ± 0.054	4.487ValThr: 4.487 ± 0.065	5.402ValVal: 5.402 ± 0.08	0.879ValTrp: 0.879 ± 0.024	1.411ValTyr: 1.411 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.339TrpAla: 1.339 ± 0.03	0.129TrpCys: 0.129 ± 0.009	0.702TrpAsp: 0.702 ± 0.022	0.634TrpGlu: 0.634 ± 0.02	0.584TrpPhe: 0.584 ± 0.021	0.96TrpGly: 0.96 ± 0.03	0.351TrpHis: 0.351 ± 0.016	0.717TrpIle: 0.717 ± 0.021	0.524TrpLys: 0.524 ± 0.023	1.769TrpLeu: 1.769 ± 0.04	0.359TrpMet: 0.359 ± 0.016	0.496TrpAsn: 0.496 ± 0.018	0.695TrpPro: 0.695 ± 0.025	0.685TrpGln: 0.685 ± 0.022	1.314TrpArg: 1.314 ± 0.035	0.912TrpSer: 0.912 ± 0.025	0.812TrpThr: 0.812 ± 0.025	0.789TrpVal: 0.789 ± 0.027	0.242TrpTrp: 0.242 ± 0.014	0.306TrpTyr: 0.306 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.639TyrAla: 2.639 ± 0.048	0.222TyrCys: 0.222 ± 0.011	1.52TyrAsp: 1.52 ± 0.036	1.204TyrGlu: 1.204 ± 0.031	0.845TyrPhe: 0.845 ± 0.026	2.191TyrGly: 2.191 ± 0.044	0.526TyrHis: 0.526 ± 0.019	0.805TyrIle: 0.805 ± 0.026	0.59TyrLys: 0.59 ± 0.022	2.168TyrLeu: 2.168 ± 0.04	0.383TyrMet: 0.383 ± 0.019	0.613TyrAsn: 0.613 ± 0.027	1.086TyrPro: 1.086 ± 0.029	0.718TyrGln: 0.718 ± 0.024	1.953TyrArg: 1.953 ± 0.04	1.193TyrSer: 1.193 ± 0.031	1.083TyrThr: 1.083 ± 0.033	1.554TyrVal: 1.554 ± 0.036	0.361TyrTrp: 0.361 ± 0.017	0.629TyrTyr: 0.629 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4481 proteins (1390953 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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