Amino acid dipepetide frequency for Bacillus sp. SJS

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.276AlaAla: 8.276 ± 0.134
0.568AlaCys: 0.568 ± 0.025
4.066AlaAsp: 4.066 ± 0.068
5.744AlaGlu: 5.744 ± 0.081
3.487AlaPhe: 3.487 ± 0.063
6.796AlaGly: 6.796 ± 0.101
1.246AlaHis: 1.246 ± 0.032
5.748AlaIle: 5.748 ± 0.072
4.916AlaLys: 4.916 ± 0.077
7.467AlaLeu: 7.467 ± 0.1
2.156AlaMet: 2.156 ± 0.047
2.667AlaAsn: 2.667 ± 0.048
2.254AlaPro: 2.254 ± 0.046
2.251AlaGln: 2.251 ± 0.049
2.819AlaArg: 2.819 ± 0.055
4.815AlaSer: 4.815 ± 0.079
2.708AlaThr: 2.708 ± 0.067
6.1AlaVal: 6.1 ± 0.08
0.633AlaTrp: 0.633 ± 0.025
2.413AlaTyr: 2.413 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.431CysAla: 0.431 ± 0.02
0.098CysCys: 0.098 ± 0.01
0.334CysAsp: 0.334 ± 0.017
0.423CysGlu: 0.423 ± 0.019
0.32CysPhe: 0.32 ± 0.017
0.632CysGly: 0.632 ± 0.026
0.18CysHis: 0.18 ± 0.014
0.472CysIle: 0.472 ± 0.018
0.348CysLys: 0.348 ± 0.017
0.695CysLeu: 0.695 ± 0.029
0.219CysMet: 0.219 ± 0.012
0.217CysAsn: 0.217 ± 0.016
0.351CysPro: 0.351 ± 0.019
0.242CysGln: 0.242 ± 0.017
0.36CysArg: 0.36 ± 0.017
0.533CysSer: 0.533 ± 0.023
0.412CysThr: 0.412 ± 0.018
0.375CysVal: 0.375 ± 0.018
0.064CysTrp: 0.064 ± 0.007
0.225CysTyr: 0.225 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.511AspAla: 3.511 ± 0.057
0.384AspCys: 0.384 ± 0.018
2.212AspAsp: 2.212 ± 0.047
4.183AspGlu: 4.183 ± 0.072
2.388AspPhe: 2.388 ± 0.047
3.547AspGly: 3.547 ± 0.066
1.211AspHis: 1.211 ± 0.034
3.437AspIle: 3.437 ± 0.061
2.819AspLys: 2.819 ± 0.051
5.353AspLeu: 5.353 ± 0.072
1.309AspMet: 1.309 ± 0.037
1.433AspAsn: 1.433 ± 0.035
2.107AspPro: 2.107 ± 0.04
2.291AspGln: 2.291 ± 0.044
2.542AspArg: 2.542 ± 0.049
2.9AspSer: 2.9 ± 0.055
2.383AspThr: 2.383 ± 0.05
3.313AspVal: 3.313 ± 0.059
0.663AspTrp: 0.663 ± 0.023
2.12AspTyr: 2.12 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
5.937GluAla: 5.937 ± 0.081
0.421GluCys: 0.421 ± 0.02
3.901GluAsp: 3.901 ± 0.059
7.184GluGlu: 7.184 ± 0.107
2.683GluPhe: 2.683 ± 0.046
4.589GluGly: 4.589 ± 0.078
1.508GluHis: 1.508 ± 0.034
5.298GluIle: 5.298 ± 0.08
6.962GluLys: 6.962 ± 0.086
7.274GluLeu: 7.274 ± 0.094
2.337GluMet: 2.337 ± 0.044
3.545GluAsn: 3.545 ± 0.058
2.118GluPro: 2.118 ± 0.052
3.232GluGln: 3.232 ± 0.052
3.637GluArg: 3.637 ± 0.065
3.818GluSer: 3.818 ± 0.058
3.984GluThr: 3.984 ± 0.06
4.552GluVal: 4.552 ± 0.073
0.909GluTrp: 0.909 ± 0.031
2.242GluTyr: 2.242 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
3.194PheAla: 3.194 ± 0.062
0.335PheCys: 0.335 ± 0.018
2.287PheAsp: 2.287 ± 0.051
2.852PheGlu: 2.852 ± 0.048
2.372PhePhe: 2.372 ± 0.053
3.397PheGly: 3.397 ± 0.061
0.992PheHis: 0.992 ± 0.032
3.386PheIle: 3.386 ± 0.067
2.527PheLys: 2.527 ± 0.041
4.566PheLeu: 4.566 ± 0.082
1.232PheMet: 1.232 ± 0.031
1.793PheAsn: 1.793 ± 0.049
1.744PhePro: 1.744 ± 0.045
1.603PheGln: 1.603 ± 0.038
1.646PheArg: 1.646 ± 0.035
3.558PheSer: 3.558 ± 0.063
2.593PheThr: 2.593 ± 0.051
2.807PheVal: 2.807 ± 0.054
0.515PheTrp: 0.515 ± 0.023
1.72PheTyr: 1.72 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
5.132GlyAla: 5.132 ± 0.09
0.615GlyCys: 0.615 ± 0.021
3.319GlyAsp: 3.319 ± 0.057
4.674GlyGlu: 4.674 ± 0.07
3.527GlyPhe: 3.527 ± 0.056
5.073GlyGly: 5.073 ± 0.085
1.397GlyHis: 1.397 ± 0.037
5.995GlyIle: 5.995 ± 0.081
5.493GlyLys: 5.493 ± 0.077
6.887GlyLeu: 6.887 ± 0.094
2.359GlyMet: 2.359 ± 0.05
2.679GlyAsn: 2.679 ± 0.053
1.809GlyPro: 1.809 ± 0.044
2.369GlyGln: 2.369 ± 0.051
2.98GlyArg: 2.98 ± 0.056
4.495GlySer: 4.495 ± 0.066
4.241GlyThr: 4.241 ± 0.062
4.704GlyVal: 4.704 ± 0.068
0.858GlyTrp: 0.858 ± 0.031
2.767GlyTyr: 2.767 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
1.448HisAla: 1.448 ± 0.036
0.172HisCys: 0.172 ± 0.012
0.98HisAsp: 0.98 ± 0.031
1.383HisGlu: 1.383 ± 0.035
1.055HisPhe: 1.055 ± 0.031
1.45HisGly: 1.45 ± 0.035
0.678HisHis: 0.678 ± 0.026
1.374HisIle: 1.374 ± 0.037
0.996HisLys: 0.996 ± 0.03
2.139HisLeu: 2.139 ± 0.048
0.558HisMet: 0.558 ± 0.023
0.69HisAsn: 0.69 ± 0.026
1.202HisPro: 1.202 ± 0.036
0.828HisGln: 0.828 ± 0.028
0.892HisArg: 0.892 ± 0.033
1.318HisSer: 1.318 ± 0.037
1.042HisThr: 1.042 ± 0.03
1.318HisVal: 1.318 ± 0.034
0.225HisTrp: 0.225 ± 0.013
0.888HisTyr: 0.888 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.844IleAla: 5.844 ± 0.079
0.624IleCys: 0.624 ± 0.024
3.717IleAsp: 3.717 ± 0.056
5.291IleGlu: 5.291 ± 0.075
3.036IlePhe: 3.036 ± 0.062
5.792IleGly: 5.792 ± 0.084
1.67IleHis: 1.67 ± 0.034
4.96IleIle: 4.96 ± 0.084
4.241IleLys: 4.241 ± 0.061
7.126IleLeu: 7.126 ± 0.09
1.844IleMet: 1.844 ± 0.039
2.664IleAsn: 2.664 ± 0.05
3.298IlePro: 3.298 ± 0.056
2.952IleGln: 2.952 ± 0.051
3.321IleArg: 3.321 ± 0.059
5.153IleSer: 5.153 ± 0.069
3.971IleThr: 3.971 ± 0.062
4.761IleVal: 4.761 ± 0.072
0.658IleTrp: 0.658 ± 0.024
2.341IleTyr: 2.341 ± 0.044
0.0IleXaa: 0.0 ± 0.0
Lys
5.211LysAla: 5.211 ± 0.076
0.304LysCys: 0.304 ± 0.017
3.983LysAsp: 3.983 ± 0.063
7.003LysGlu: 7.003 ± 0.096
1.89LysPhe: 1.89 ± 0.041
4.812LysGly: 4.812 ± 0.062
1.29LysHis: 1.29 ± 0.035
4.44LysIle: 4.44 ± 0.059
5.944LysLys: 5.944 ± 0.081
5.841LysLeu: 5.841 ± 0.067
2.333LysMet: 2.333 ± 0.044
3.349LysAsn: 3.349 ± 0.055
2.568LysPro: 2.568 ± 0.045
2.975LysGln: 2.975 ± 0.055
3.382LysArg: 3.382 ± 0.054
3.696LysSer: 3.696 ± 0.062
3.857LysThr: 3.857 ± 0.058
4.255LysVal: 4.255 ± 0.072
0.863LysTrp: 0.863 ± 0.028
1.961LysTyr: 1.961 ± 0.042
0.0LysXaa: 0.0 ± 0.0
Leu
7.861LeuAla: 7.861 ± 0.101
0.647LeuCys: 0.647 ± 0.025
4.809LeuAsp: 4.809 ± 0.067
6.843LeuGlu: 6.843 ± 0.086
4.886LeuPhe: 4.886 ± 0.094
6.235LeuGly: 6.235 ± 0.077
1.991LeuHis: 1.991 ± 0.043
7.153LeuIle: 7.153 ± 0.11
7.037LeuLys: 7.037 ± 0.086
10.106LeuLeu: 10.106 ± 0.136
2.672LeuMet: 2.672 ± 0.05
4.306LeuAsn: 4.306 ± 0.062
4.066LeuPro: 4.066 ± 0.065
3.507LeuGln: 3.507 ± 0.051
3.491LeuArg: 3.491 ± 0.064
7.135LeuSer: 7.135 ± 0.081
5.669LeuThr: 5.669 ± 0.075
5.6LeuVal: 5.6 ± 0.072
0.844LeuTrp: 0.844 ± 0.025
3.176LeuTyr: 3.176 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
2.279MetAla: 2.279 ± 0.048
0.153MetCys: 0.153 ± 0.012
1.655MetAsp: 1.655 ± 0.041
2.318MetGlu: 2.318 ± 0.049
1.055MetPhe: 1.055 ± 0.032
1.914MetGly: 1.914 ± 0.044
0.448MetHis: 0.448 ± 0.019
2.234MetIle: 2.234 ± 0.041
2.661MetLys: 2.661 ± 0.049
2.68MetLeu: 2.68 ± 0.057
0.917MetMet: 0.917 ± 0.031
1.671MetAsn: 1.671 ± 0.036
1.11MetPro: 1.11 ± 0.039
0.884MetGln: 0.884 ± 0.03
1.108MetArg: 1.108 ± 0.028
1.631MetSer: 1.631 ± 0.04
1.761MetThr: 1.761 ± 0.039
1.642MetVal: 1.642 ± 0.04
0.166MetTrp: 0.166 ± 0.012
0.743MetTyr: 0.743 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
2.803AsnAla: 2.803 ± 0.051
0.258AsnCys: 0.258 ± 0.014
1.901AsnAsp: 1.901 ± 0.044
3.358AsnGlu: 3.358 ± 0.053
1.476AsnPhe: 1.476 ± 0.04
3.394AsnGly: 3.394 ± 0.063
0.938AsnHis: 0.938 ± 0.027
2.682AsnIle: 2.682 ± 0.051
2.628AsnLys: 2.628 ± 0.062
3.705AsnLeu: 3.705 ± 0.053
1.153AsnMet: 1.153 ± 0.033
1.465AsnAsn: 1.465 ± 0.037
2.212AsnPro: 2.212 ± 0.045
1.759AsnGln: 1.759 ± 0.047
2.093AsnArg: 2.093 ± 0.042
2.258AsnSer: 2.258 ± 0.049
1.935AsnThr: 1.935 ± 0.046
2.637AsnVal: 2.637 ± 0.052
0.501AsnTrp: 0.501 ± 0.02
1.32AsnTyr: 1.32 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
3.098ProAla: 3.098 ± 0.057
0.224ProCys: 0.224 ± 0.013
2.264ProAsp: 2.264 ± 0.048
3.437ProGlu: 3.437 ± 0.06
2.082ProPhe: 2.082 ± 0.044
2.699ProGly: 2.699 ± 0.056
0.765ProHis: 0.765 ± 0.027
2.658ProIle: 2.658 ± 0.045
2.151ProLys: 2.151 ± 0.045
3.59ProLeu: 3.59 ± 0.049
0.825ProMet: 0.825 ± 0.026
1.515ProAsn: 1.515 ± 0.037
1.098ProPro: 1.098 ± 0.035
1.105ProGln: 1.105 ± 0.036
1.13ProArg: 1.13 ± 0.03
2.525ProSer: 2.525 ± 0.053
1.411ProThr: 1.411 ± 0.035
3.066ProVal: 3.066 ± 0.056
0.331ProTrp: 0.331 ± 0.017
1.475ProTyr: 1.475 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.953GlnAla: 2.953 ± 0.05
0.2GlnCys: 0.2 ± 0.014
1.66GlnAsp: 1.66 ± 0.041
2.753GlnGlu: 2.753 ± 0.054
1.567GlnPhe: 1.567 ± 0.036
2.122GlnGly: 2.122 ± 0.053
0.762GlnHis: 0.762 ± 0.025
2.485GlnIle: 2.485 ± 0.047
2.741GlnLys: 2.741 ± 0.048
3.751GlnLeu: 3.751 ± 0.063
1.196GlnMet: 1.196 ± 0.032
1.646GlnAsn: 1.646 ± 0.044
1.305GlnPro: 1.305 ± 0.034
1.608GlnGln: 1.608 ± 0.043
1.445GlnArg: 1.445 ± 0.039
2.362GlnSer: 2.362 ± 0.053
2.145GlnThr: 2.145 ± 0.048
2.194GlnVal: 2.194 ± 0.051
0.413GlnTrp: 0.413 ± 0.019
1.312GlnTyr: 1.312 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
2.604ArgAla: 2.604 ± 0.049
0.248ArgCys: 0.248 ± 0.014
2.083ArgAsp: 2.083 ± 0.043
3.431ArgGlu: 3.431 ± 0.062
1.978ArgPhe: 1.978 ± 0.048
2.435ArgGly: 2.435 ± 0.051
0.849ArgHis: 0.849 ± 0.026
3.302ArgIle: 3.302 ± 0.059
3.331ArgLys: 3.331 ± 0.056
4.2ArgLeu: 4.2 ± 0.071
1.411ArgMet: 1.411 ± 0.035
1.902ArgAsn: 1.902 ± 0.042
1.392ArgPro: 1.392 ± 0.033
1.501ArgGln: 1.501 ± 0.042
1.883ArgArg: 1.883 ± 0.047
2.556ArgSer: 2.556 ± 0.048
2.252ArgThr: 2.252 ± 0.043
2.63ArgVal: 2.63 ± 0.045
0.417ArgTrp: 0.417 ± 0.017
1.575ArgTyr: 1.575 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
4.687SerAla: 4.687 ± 0.074
0.443SerCys: 0.443 ± 0.022
2.894SerAsp: 2.894 ± 0.049
4.446SerGlu: 4.446 ± 0.067
3.471SerPhe: 3.471 ± 0.06
5.03SerGly: 5.03 ± 0.072
1.295SerHis: 1.295 ± 0.032
5.212SerIle: 5.212 ± 0.078
4.283SerLys: 4.283 ± 0.057
6.481SerLeu: 6.481 ± 0.079
1.894SerMet: 1.894 ± 0.044
2.353SerAsn: 2.353 ± 0.049
2.259SerPro: 2.259 ± 0.047
2.1SerGln: 2.1 ± 0.044
2.688SerArg: 2.688 ± 0.056
4.65SerSer: 4.65 ± 0.073
2.84SerThr: 2.84 ± 0.051
4.243SerVal: 4.243 ± 0.066
0.633SerTrp: 0.633 ± 0.028
2.322SerTyr: 2.322 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
4.753ThrAla: 4.753 ± 0.076
0.312ThrCys: 0.312 ± 0.018
2.774ThrAsp: 2.774 ± 0.053
3.57ThrGlu: 3.57 ± 0.055
2.359ThrPhe: 2.359 ± 0.05
4.716ThrGly: 4.716 ± 0.077
0.955ThrHis: 0.955 ± 0.03
4.037ThrIle: 4.037 ± 0.054
3.09ThrLys: 3.09 ± 0.058
4.777ThrLeu: 4.777 ± 0.066
1.345ThrMet: 1.345 ± 0.037
2.022ThrAsn: 2.022 ± 0.047
2.165ThrPro: 2.165 ± 0.043
1.333ThrGln: 1.333 ± 0.035
1.818ThrArg: 1.818 ± 0.042
3.225ThrSer: 3.225 ± 0.061
2.21ThrThr: 2.21 ± 0.051
4.092ThrVal: 4.092 ± 0.068
0.493ThrTrp: 0.493 ± 0.021
1.763ThrTyr: 1.763 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
4.265ValAla: 4.265 ± 0.081
0.561ValCys: 0.561 ± 0.026
2.866ValAsp: 2.866 ± 0.047
4.01ValGlu: 4.01 ± 0.065
3.144ValPhe: 3.144 ± 0.053
3.54ValGly: 3.54 ± 0.06
1.447ValHis: 1.447 ± 0.035
5.337ValIle: 5.337 ± 0.077
4.843ValLys: 4.843 ± 0.066
6.86ValLeu: 6.86 ± 0.093
1.929ValMet: 1.929 ± 0.048
2.762ValAsn: 2.762 ± 0.05
2.728ValPro: 2.728 ± 0.05
2.41ValGln: 2.41 ± 0.044
2.667ValArg: 2.667 ± 0.052
4.651ValSer: 4.651 ± 0.059
3.944ValThr: 3.944 ± 0.064
4.064ValVal: 4.064 ± 0.071
0.638ValTrp: 0.638 ± 0.027
2.324ValTyr: 2.324 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.627TrpAla: 0.627 ± 0.026
0.083TrpCys: 0.083 ± 0.008
0.534TrpAsp: 0.534 ± 0.022
0.661TrpGlu: 0.661 ± 0.027
0.537TrpPhe: 0.537 ± 0.023
0.664TrpGly: 0.664 ± 0.027
0.216TrpHis: 0.216 ± 0.014
0.875TrpIle: 0.875 ± 0.027
0.803TrpLys: 0.803 ± 0.026
1.149TrpLeu: 1.149 ± 0.037
0.407TrpMet: 0.407 ± 0.019
0.548TrpAsn: 0.548 ± 0.022
0.274TrpPro: 0.274 ± 0.016
0.322TrpGln: 0.322 ± 0.017
0.4TrpArg: 0.4 ± 0.018
0.63TrpSer: 0.63 ± 0.026
0.56TrpThr: 0.56 ± 0.022
0.579TrpVal: 0.579 ± 0.021
0.134TrpTrp: 0.134 ± 0.011
0.344TrpTyr: 0.344 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.215TyrAla: 2.215 ± 0.046
0.278TyrCys: 0.278 ± 0.017
1.869TyrAsp: 1.869 ± 0.05
2.566TyrGlu: 2.566 ± 0.05
1.808TyrPhe: 1.808 ± 0.038
2.536TyrGly: 2.536 ± 0.057
0.799TyrHis: 0.799 ± 0.026
2.181TyrIle: 2.181 ± 0.045
2.144TyrLys: 2.144 ± 0.043
3.386TyrLeu: 3.386 ± 0.056
0.935TyrMet: 0.935 ± 0.029
1.248TyrAsn: 1.248 ± 0.039
1.392TyrPro: 1.392 ± 0.037
1.349TyrGln: 1.349 ± 0.033
1.679TyrArg: 1.679 ± 0.039
2.313TyrSer: 2.313 ± 0.05
1.891TyrThr: 1.891 ± 0.046
2.057TyrVal: 2.057 ± 0.044
0.389TyrTrp: 0.389 ± 0.019
1.36TyrTyr: 1.36 ± 0.041
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4050 proteins (1163448 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski