Wuhan cricket virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0QKT8|A0A0P0QKT8_9VIRU Putative glycoprotein OS=Wuhan cricket virus OX=1746070 GN=VP1 PE=4 SV=1
MM1 pKa = 7.58GSYY4 pKa = 8.83WCSNFALLWVQKK16 pKa = 9.93RR17 pKa = 11.84SSHH20 pKa = 6.49SYY22 pKa = 10.6DD23 pKa = 2.85IRR25 pKa = 11.84CCLFLCLSLWTEE37 pKa = 4.31FSQAGDD43 pKa = 3.56LEE45 pKa = 4.34YY46 pKa = 10.87LSRR49 pKa = 11.84GLKK52 pKa = 9.93DD53 pKa = 3.05VRR55 pKa = 11.84GFMTTDD61 pKa = 2.65NVYY64 pKa = 10.46INTTTRR70 pKa = 11.84VQDD73 pKa = 4.14FSEE76 pKa = 3.95SRR78 pKa = 11.84YY79 pKa = 9.81EE80 pKa = 4.0VLVHH84 pKa = 7.19NITEE88 pKa = 4.22KK89 pKa = 10.52MLTPTEE95 pKa = 3.94FMVYY99 pKa = 8.96DD100 pKa = 4.47TDD102 pKa = 5.39AGDD105 pKa = 4.1PFCTDD110 pKa = 2.96YY111 pKa = 10.74LTYY114 pKa = 10.85DD115 pKa = 3.44VGWFSKK121 pKa = 10.93NNPTKK126 pKa = 10.57EE127 pKa = 4.41CVNSLARR134 pKa = 11.84SNCFYY139 pKa = 10.78LWHH142 pKa = 6.32NHH144 pKa = 6.17DD145 pKa = 5.18DD146 pKa = 4.35YY147 pKa = 11.44EE148 pKa = 4.66ISWFEE153 pKa = 3.85NVHH156 pKa = 5.36TLVCVGIRR164 pKa = 11.84YY165 pKa = 9.53SKK167 pKa = 10.75SQITSRR173 pKa = 11.84KK174 pKa = 7.43NSHH177 pKa = 4.67WSRR180 pKa = 11.84WYY182 pKa = 10.2SLTRR186 pKa = 11.84AEE188 pKa = 4.37PTSILQYY195 pKa = 11.61GMIFKK200 pKa = 10.57NGEE203 pKa = 3.99YY204 pKa = 10.53HH205 pKa = 7.27LMSPMALEE213 pKa = 4.34FGLVYY218 pKa = 10.65DD219 pKa = 4.54LSRR222 pKa = 11.84NDD224 pKa = 3.64FYY226 pKa = 11.72GYY228 pKa = 8.94ATNITHH234 pKa = 7.56ALVLEE239 pKa = 4.04HH240 pKa = 7.19RR241 pKa = 11.84NLIIGSSPTLPAEE254 pKa = 4.72FKK256 pKa = 10.62HH257 pKa = 5.43ITYY260 pKa = 7.62TTLGKK265 pKa = 8.91KK266 pKa = 7.68TVFCNDD272 pKa = 4.05RR273 pKa = 11.84IVGNPIDD280 pKa = 5.26DD281 pKa = 4.22NEE283 pKa = 4.37SDD285 pKa = 3.86EE286 pKa = 5.62VIATDD291 pKa = 4.58FNCDD295 pKa = 2.99PHH297 pKa = 7.56QVLGVIQRR305 pKa = 11.84PNGDD309 pKa = 4.17LLPVYY314 pKa = 9.6WCLQWNCVNTKK325 pKa = 10.4SFGDD329 pKa = 3.83LFTTEE334 pKa = 4.44LNRR337 pKa = 11.84KK338 pKa = 9.44FEE340 pKa = 4.84DD341 pKa = 4.04DD342 pKa = 3.22EE343 pKa = 5.02HH344 pKa = 7.56EE345 pKa = 4.28NFAVRR350 pKa = 11.84ILLIIRR356 pKa = 11.84SLIVKK361 pKa = 8.08ATLYY365 pKa = 8.82ITQSINLRR373 pKa = 11.84VFSLSVWGIFTTIMVIRR390 pKa = 11.84LTGSWIAGTIMGLYY404 pKa = 10.01VVVLLSDD411 pKa = 3.79

Molecular weight: 47.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0QKL9|A0A0P0QKL9_9VIRU Uncharacterized protein OS=Wuhan cricket virus OX=1746070 GN=VP3 PE=4 SV=1
MM1 pKa = 7.66NITKK5 pKa = 9.9VLLVALVGIHH15 pKa = 6.66FGGTAYY21 pKa = 10.56AATNTGASVAKK32 pKa = 9.29LTDD35 pKa = 2.65IWSIGSVSKK44 pKa = 10.48SWNPSDD50 pKa = 3.52WSDD53 pKa = 3.46DD54 pKa = 3.5FKK56 pKa = 11.66LRR58 pKa = 11.84VYY60 pKa = 10.73QSLTLVIAIAFLGGTMAHH78 pKa = 6.53MIVGFVVGIGLVHH91 pKa = 5.73NTWVLTGALILLYY104 pKa = 10.11YY105 pKa = 9.85GYY107 pKa = 11.0KK108 pKa = 9.65RR109 pKa = 11.84GHH111 pKa = 7.37PIAMILGAVCSYY123 pKa = 11.13VLAFGQSLAKK133 pKa = 10.61LGII136 pKa = 3.94

Molecular weight: 14.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3094

136

943

515.7

58.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.527 ± 0.698

1.584 ± 0.321

4.622 ± 0.425

4.848 ± 0.62

3.943 ± 0.347

7.531 ± 0.736

2.618 ± 0.435

6.981 ± 0.438

4.654 ± 0.671

10.116 ± 0.318

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.846 ± 0.315

4.396 ± 0.363

4.525 ± 0.558

2.521 ± 0.199

5.139 ± 0.717

7.789 ± 1.051

6.27 ± 0.427

6.755 ± 0.458

1.875 ± 0.322

4.46 ± 0.367
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski