Amino acid dipepetide frequency for Staphylococcus phage SA780ruMSSAST101

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.334AlaAla: 1.334 ± 0.621
0.392AlaCys: 0.392 ± 0.157
2.432AlaAsp: 2.432 ± 0.519
3.295AlaGlu: 3.295 ± 0.665
2.275AlaPhe: 2.275 ± 0.506
3.766AlaGly: 3.766 ± 0.778
0.235AlaHis: 0.235 ± 0.141
4.786AlaIle: 4.786 ± 0.694
4.864AlaLys: 4.864 ± 0.51
5.1AlaLeu: 5.1 ± 0.718
1.726AlaMet: 1.726 ± 0.3
3.923AlaAsn: 3.923 ± 0.499
1.648AlaPro: 1.648 ± 0.29
2.903AlaGln: 2.903 ± 0.508
3.06AlaArg: 3.06 ± 0.504
4.237AlaSer: 4.237 ± 0.582
3.295AlaThr: 3.295 ± 0.572
2.903AlaVal: 2.903 ± 0.52
0.471AlaTrp: 0.471 ± 0.22
2.432AlaTyr: 2.432 ± 0.362
0.0AlaXaa: 0.0 ± 0.0
Cys
0.235CysAla: 0.235 ± 0.132
0.078CysCys: 0.078 ± 0.07
0.235CysAsp: 0.235 ± 0.161
0.157CysGlu: 0.157 ± 0.122
0.235CysPhe: 0.235 ± 0.151
0.235CysGly: 0.235 ± 0.156
0.157CysHis: 0.157 ± 0.102
0.471CysIle: 0.471 ± 0.169
0.314CysLys: 0.314 ± 0.168
0.392CysLeu: 0.392 ± 0.131
0.0CysMet: 0.0 ± 0.0
0.392CysAsn: 0.392 ± 0.174
0.0CysPro: 0.0 ± 0.0
0.0CysGln: 0.0 ± 0.0
0.392CysArg: 0.392 ± 0.228
0.392CysSer: 0.392 ± 0.195
0.471CysThr: 0.471 ± 0.188
0.314CysVal: 0.314 ± 0.173
0.0CysTrp: 0.0 ± 0.0
0.549CysTyr: 0.549 ± 0.212
0.0CysXaa: 0.0 ± 0.0
Asp
2.981AspAla: 2.981 ± 0.375
0.314AspCys: 0.314 ± 0.166
4.237AspAsp: 4.237 ± 0.754
5.413AspGlu: 5.413 ± 0.841
2.824AspPhe: 2.824 ± 0.512
4.55AspGly: 4.55 ± 0.576
1.098AspHis: 1.098 ± 0.265
4.629AspIle: 4.629 ± 0.591
5.57AspLys: 5.57 ± 0.672
4.943AspLeu: 4.943 ± 0.699
1.334AspMet: 1.334 ± 0.309
4.629AspAsn: 4.629 ± 0.583
1.412AspPro: 1.412 ± 0.378
1.02AspGln: 1.02 ± 0.324
2.354AspArg: 2.354 ± 0.457
3.609AspSer: 3.609 ± 0.494
3.844AspThr: 3.844 ± 0.649
3.923AspVal: 3.923 ± 0.509
0.471AspTrp: 0.471 ± 0.207
3.374AspTyr: 3.374 ± 0.62
0.0AspXaa: 0.0 ± 0.0
Glu
4.786GluAla: 4.786 ± 0.838
0.157GluCys: 0.157 ± 0.1
3.138GluAsp: 3.138 ± 0.444
6.983GluGlu: 6.983 ± 0.955
3.452GluPhe: 3.452 ± 0.547
2.354GluGly: 2.354 ± 0.319
1.255GluHis: 1.255 ± 0.333
7.532GluIle: 7.532 ± 0.959
6.512GluLys: 6.512 ± 0.986
8.159GluLeu: 8.159 ± 0.958
2.511GluMet: 2.511 ± 0.454
5.413GluAsn: 5.413 ± 0.632
1.961GluPro: 1.961 ± 0.399
3.844GluGln: 3.844 ± 0.624
3.844GluArg: 3.844 ± 0.603
4.472GluSer: 4.472 ± 0.488
3.609GluThr: 3.609 ± 0.479
4.08GluVal: 4.08 ± 0.622
0.785GluTrp: 0.785 ± 0.288
3.687GluTyr: 3.687 ± 0.624
0.0GluXaa: 0.0 ± 0.0
Phe
2.04PheAla: 2.04 ± 0.382
0.157PheCys: 0.157 ± 0.112
3.217PheAsp: 3.217 ± 0.531
3.06PheGlu: 3.06 ± 0.439
1.098PhePhe: 1.098 ± 0.321
2.668PheGly: 2.668 ± 0.576
0.706PheHis: 0.706 ± 0.248
3.531PheIle: 3.531 ± 0.599
4.472PheLys: 4.472 ± 0.467
2.824PheLeu: 2.824 ± 0.616
1.334PheMet: 1.334 ± 0.396
4.786PheAsn: 4.786 ± 0.528
0.628PhePro: 0.628 ± 0.264
0.941PheGln: 0.941 ± 0.251
1.334PheArg: 1.334 ± 0.309
1.883PheSer: 1.883 ± 0.44
2.118PheThr: 2.118 ± 0.411
2.275PheVal: 2.275 ± 0.421
0.157PheTrp: 0.157 ± 0.106
1.569PheTyr: 1.569 ± 0.334
0.0PheXaa: 0.0 ± 0.0
Gly
2.903GlyAla: 2.903 ± 0.619
0.314GlyCys: 0.314 ± 0.176
2.746GlyAsp: 2.746 ± 0.376
3.06GlyGlu: 3.06 ± 0.483
2.511GlyPhe: 2.511 ± 0.469
2.903GlyGly: 2.903 ± 0.836
1.334GlyHis: 1.334 ± 0.33
4.08GlyIle: 4.08 ± 0.795
5.492GlyLys: 5.492 ± 0.715
5.727GlyLeu: 5.727 ± 0.854
1.177GlyMet: 1.177 ± 0.315
3.687GlyAsn: 3.687 ± 0.55
0.628GlyPro: 0.628 ± 0.266
2.432GlyGln: 2.432 ± 0.472
2.668GlyArg: 2.668 ± 0.43
3.531GlySer: 3.531 ± 0.495
2.511GlyThr: 2.511 ± 0.531
2.981GlyVal: 2.981 ± 0.686
0.863GlyTrp: 0.863 ± 0.255
2.903GlyTyr: 2.903 ± 0.567
0.0GlyXaa: 0.0 ± 0.0
His
0.706HisAla: 0.706 ± 0.23
0.157HisCys: 0.157 ± 0.099
0.941HisAsp: 0.941 ± 0.297
1.804HisGlu: 1.804 ± 0.433
1.255HisPhe: 1.255 ± 0.253
0.785HisGly: 0.785 ± 0.278
0.235HisHis: 0.235 ± 0.134
1.648HisIle: 1.648 ± 0.418
1.412HisLys: 1.412 ± 0.308
1.255HisLeu: 1.255 ± 0.3
0.471HisMet: 0.471 ± 0.201
1.098HisAsn: 1.098 ± 0.307
0.471HisPro: 0.471 ± 0.203
0.863HisGln: 0.863 ± 0.283
0.628HisArg: 0.628 ± 0.218
0.863HisSer: 0.863 ± 0.223
1.098HisThr: 1.098 ± 0.257
1.177HisVal: 1.177 ± 0.311
0.157HisTrp: 0.157 ± 0.112
0.706HisTyr: 0.706 ± 0.259
0.0HisXaa: 0.0 ± 0.0
Ile
5.021IleAla: 5.021 ± 0.577
0.314IleCys: 0.314 ± 0.155
5.492IleAsp: 5.492 ± 0.723
7.453IleGlu: 7.453 ± 0.828
3.217IlePhe: 3.217 ± 0.657
3.844IleGly: 3.844 ± 0.548
1.412IleHis: 1.412 ± 0.371
5.335IleIle: 5.335 ± 0.608
7.453IleLys: 7.453 ± 0.776
5.021IleLeu: 5.021 ± 0.733
1.883IleMet: 1.883 ± 0.321
6.12IleAsn: 6.12 ± 1.022
2.354IlePro: 2.354 ± 0.486
3.374IleGln: 3.374 ± 0.549
2.981IleArg: 2.981 ± 0.502
4.864IleSer: 4.864 ± 0.699
5.335IleThr: 5.335 ± 0.518
4.864IleVal: 4.864 ± 0.448
1.098IleTrp: 1.098 ± 0.405
2.668IleTyr: 2.668 ± 0.537
0.0IleXaa: 0.0 ± 0.0
Lys
5.492LysAla: 5.492 ± 0.774
0.078LysCys: 0.078 ± 0.084
5.178LysAsp: 5.178 ± 0.46
8.003LysGlu: 8.003 ± 0.966
3.923LysPhe: 3.923 ± 0.545
5.649LysGly: 5.649 ± 0.706
1.569LysHis: 1.569 ± 0.327
6.983LysIle: 6.983 ± 0.738
7.846LysLys: 7.846 ± 0.996
6.904LysLeu: 6.904 ± 0.817
1.961LysMet: 1.961 ± 0.34
4.394LysAsn: 4.394 ± 0.411
3.138LysPro: 3.138 ± 0.506
4.864LysGln: 4.864 ± 0.78
4.394LysArg: 4.394 ± 0.487
5.963LysSer: 5.963 ± 0.69
6.12LysThr: 6.12 ± 0.785
5.335LysVal: 5.335 ± 0.55
1.02LysTrp: 1.02 ± 0.294
3.687LysTyr: 3.687 ± 0.533
0.0LysXaa: 0.0 ± 0.0
Leu
4.629LeuAla: 4.629 ± 0.593
0.392LeuCys: 0.392 ± 0.164
6.041LeuAsp: 6.041 ± 0.836
7.061LeuGlu: 7.061 ± 0.877
3.687LeuPhe: 3.687 ± 0.503
3.687LeuGly: 3.687 ± 0.747
1.02LeuHis: 1.02 ± 0.249
5.335LeuIle: 5.335 ± 0.599
8.552LeuLys: 8.552 ± 0.687
7.218LeuLeu: 7.218 ± 1.034
1.883LeuMet: 1.883 ± 0.373
5.649LeuAsn: 5.649 ± 0.576
2.354LeuPro: 2.354 ± 0.369
3.609LeuGln: 3.609 ± 0.511
3.923LeuArg: 3.923 ± 0.518
5.178LeuSer: 5.178 ± 0.756
4.394LeuThr: 4.394 ± 0.602
4.237LeuVal: 4.237 ± 0.485
0.706LeuTrp: 0.706 ± 0.229
2.354LeuTyr: 2.354 ± 0.442
0.0LeuXaa: 0.0 ± 0.0
Met
1.255MetAla: 1.255 ± 0.315
0.078MetCys: 0.078 ± 0.079
0.941MetAsp: 0.941 ± 0.232
1.804MetGlu: 1.804 ± 0.34
0.941MetPhe: 0.941 ± 0.222
1.02MetGly: 1.02 ± 0.381
0.314MetHis: 0.314 ± 0.131
2.354MetIle: 2.354 ± 0.366
2.04MetLys: 2.04 ± 0.416
1.961MetLeu: 1.961 ± 0.398
0.863MetMet: 0.863 ± 0.245
1.569MetAsn: 1.569 ± 0.383
1.255MetPro: 1.255 ± 0.465
1.255MetGln: 1.255 ± 0.345
0.785MetArg: 0.785 ± 0.286
1.883MetSer: 1.883 ± 0.336
1.883MetThr: 1.883 ± 0.348
1.412MetVal: 1.412 ± 0.296
0.314MetTrp: 0.314 ± 0.15
0.941MetTyr: 0.941 ± 0.307
0.0MetXaa: 0.0 ± 0.0
Asn
3.923AsnAla: 3.923 ± 0.563
0.078AsnCys: 0.078 ± 0.061
4.55AsnAsp: 4.55 ± 0.736
6.12AsnGlu: 6.12 ± 0.819
2.118AsnPhe: 2.118 ± 0.398
4.943AsnGly: 4.943 ± 0.627
1.177AsnHis: 1.177 ± 0.266
4.786AsnIle: 4.786 ± 0.588
6.041AsnLys: 6.041 ± 0.686
5.492AsnLeu: 5.492 ± 0.615
1.255AsnMet: 1.255 ± 0.246
4.629AsnAsn: 4.629 ± 0.728
2.511AsnPro: 2.511 ± 0.321
3.374AsnGln: 3.374 ± 0.501
2.746AsnArg: 2.746 ± 0.363
2.981AsnSer: 2.981 ± 0.514
3.452AsnThr: 3.452 ± 0.423
4.158AsnVal: 4.158 ± 0.671
1.02AsnTrp: 1.02 ± 0.382
2.903AsnTyr: 2.903 ± 0.489
0.0AsnXaa: 0.0 ± 0.0
Pro
1.255ProAla: 1.255 ± 0.295
0.0ProCys: 0.0 ± 0.0
1.726ProAsp: 1.726 ± 0.456
1.255ProGlu: 1.255 ± 0.319
0.785ProPhe: 0.785 ± 0.28
1.177ProGly: 1.177 ± 0.303
0.863ProHis: 0.863 ± 0.285
2.903ProIle: 2.903 ± 0.364
2.04ProLys: 2.04 ± 0.583
2.118ProLeu: 2.118 ± 0.5
0.941ProMet: 0.941 ± 0.295
1.804ProAsn: 1.804 ± 0.449
1.02ProPro: 1.02 ± 0.218
0.785ProGln: 0.785 ± 0.225
1.02ProArg: 1.02 ± 0.319
1.177ProSer: 1.177 ± 0.317
1.648ProThr: 1.648 ± 0.345
1.412ProVal: 1.412 ± 0.302
0.157ProTrp: 0.157 ± 0.088
1.255ProTyr: 1.255 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
3.531GlnAla: 3.531 ± 0.399
0.314GlnCys: 0.314 ± 0.152
2.746GlnAsp: 2.746 ± 0.561
3.295GlnGlu: 3.295 ± 0.768
0.941GlnPhe: 0.941 ± 0.249
1.726GlnGly: 1.726 ± 0.346
0.863GlnHis: 0.863 ± 0.281
2.824GlnIle: 2.824 ± 0.389
3.687GlnLys: 3.687 ± 0.579
3.217GlnLeu: 3.217 ± 0.588
0.941GlnMet: 0.941 ± 0.306
3.06GlnAsn: 3.06 ± 0.483
0.706GlnPro: 0.706 ± 0.173
1.726GlnGln: 1.726 ± 0.505
1.961GlnArg: 1.961 ± 0.373
2.118GlnSer: 2.118 ± 0.559
2.668GlnThr: 2.668 ± 0.478
1.961GlnVal: 1.961 ± 0.309
0.392GlnTrp: 0.392 ± 0.17
2.354GlnTyr: 2.354 ± 0.399
0.0GlnXaa: 0.0 ± 0.0
Arg
2.511ArgAla: 2.511 ± 0.388
0.471ArgCys: 0.471 ± 0.249
2.824ArgAsp: 2.824 ± 0.468
3.609ArgGlu: 3.609 ± 0.566
1.726ArgPhe: 1.726 ± 0.409
1.726ArgGly: 1.726 ± 0.451
1.177ArgHis: 1.177 ± 0.274
3.687ArgIle: 3.687 ± 0.534
3.766ArgLys: 3.766 ± 0.696
3.766ArgLeu: 3.766 ± 0.649
0.941ArgMet: 0.941 ± 0.289
2.589ArgAsn: 2.589 ± 0.386
0.628ArgPro: 0.628 ± 0.186
1.412ArgGln: 1.412 ± 0.397
2.197ArgArg: 2.197 ± 0.446
2.197ArgSer: 2.197 ± 0.389
3.138ArgThr: 3.138 ± 0.513
2.432ArgVal: 2.432 ± 0.454
0.628ArgTrp: 0.628 ± 0.268
1.961ArgTyr: 1.961 ± 0.488
0.0ArgXaa: 0.0 ± 0.0
Ser
3.374SerAla: 3.374 ± 0.635
0.471SerCys: 0.471 ± 0.258
5.021SerAsp: 5.021 ± 0.76
5.335SerGlu: 5.335 ± 0.706
2.354SerPhe: 2.354 ± 0.515
3.217SerGly: 3.217 ± 0.515
1.569SerHis: 1.569 ± 0.343
6.12SerIle: 6.12 ± 0.585
5.649SerLys: 5.649 ± 0.783
3.844SerLeu: 3.844 ± 0.444
1.491SerMet: 1.491 ± 0.355
4.629SerAsn: 4.629 ± 0.595
0.785SerPro: 0.785 ± 0.195
2.118SerGln: 2.118 ± 0.444
2.275SerArg: 2.275 ± 0.335
3.295SerSer: 3.295 ± 0.721
3.06SerThr: 3.06 ± 0.442
2.511SerVal: 2.511 ± 0.602
0.628SerTrp: 0.628 ± 0.25
2.354SerTyr: 2.354 ± 0.489
0.0SerXaa: 0.0 ± 0.0
Thr
3.844ThrAla: 3.844 ± 0.459
0.392ThrCys: 0.392 ± 0.153
4.158ThrAsp: 4.158 ± 0.649
3.452ThrGlu: 3.452 ± 0.516
2.197ThrPhe: 2.197 ± 0.379
4.394ThrGly: 4.394 ± 0.736
1.491ThrHis: 1.491 ± 0.3
4.864ThrIle: 4.864 ± 0.475
5.57ThrLys: 5.57 ± 0.52
4.394ThrLeu: 4.394 ± 0.406
1.02ThrMet: 1.02 ± 0.297
2.589ThrAsn: 2.589 ± 0.531
1.648ThrPro: 1.648 ± 0.342
2.04ThrGln: 2.04 ± 0.397
2.746ThrArg: 2.746 ± 0.615
4.472ThrSer: 4.472 ± 0.849
3.295ThrThr: 3.295 ± 0.451
3.687ThrVal: 3.687 ± 0.446
1.098ThrTrp: 1.098 ± 0.293
1.961ThrTyr: 1.961 ± 0.402
0.0ThrXaa: 0.0 ± 0.0
Val
2.746ValAla: 2.746 ± 0.44
0.392ValCys: 0.392 ± 0.228
3.452ValAsp: 3.452 ± 0.452
3.374ValGlu: 3.374 ± 0.567
2.511ValPhe: 2.511 ± 0.538
3.687ValGly: 3.687 ± 0.643
0.549ValHis: 0.549 ± 0.16
3.766ValIle: 3.766 ± 0.365
5.727ValLys: 5.727 ± 0.793
5.021ValLeu: 5.021 ± 0.703
1.412ValMet: 1.412 ± 0.395
4.237ValAsn: 4.237 ± 0.472
1.255ValPro: 1.255 ± 0.294
2.04ValGln: 2.04 ± 0.398
2.118ValArg: 2.118 ± 0.42
3.609ValSer: 3.609 ± 0.499
4.864ValThr: 4.864 ± 0.583
3.923ValVal: 3.923 ± 0.677
0.392ValTrp: 0.392 ± 0.149
1.726ValTyr: 1.726 ± 0.381
0.0ValXaa: 0.0 ± 0.0
Trp
0.314TrpAla: 0.314 ± 0.181
0.078TrpCys: 0.078 ± 0.08
0.628TrpAsp: 0.628 ± 0.294
0.628TrpGlu: 0.628 ± 0.19
0.706TrpPhe: 0.706 ± 0.23
0.314TrpGly: 0.314 ± 0.147
0.078TrpHis: 0.078 ± 0.08
1.02TrpIle: 1.02 ± 0.256
0.785TrpLys: 0.785 ± 0.229
1.098TrpLeu: 1.098 ± 0.303
0.549TrpMet: 0.549 ± 0.179
0.941TrpAsn: 0.941 ± 0.338
0.157TrpPro: 0.157 ± 0.088
0.471TrpGln: 0.471 ± 0.166
0.471TrpArg: 0.471 ± 0.144
0.628TrpSer: 0.628 ± 0.248
0.471TrpThr: 0.471 ± 0.136
0.863TrpVal: 0.863 ± 0.233
0.157TrpTrp: 0.157 ± 0.099
0.471TrpTyr: 0.471 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.197TyrAla: 2.197 ± 0.296
0.392TyrCys: 0.392 ± 0.183
2.824TyrAsp: 2.824 ± 0.407
3.374TyrGlu: 3.374 ± 0.578
2.04TyrPhe: 2.04 ± 0.458
1.804TyrGly: 1.804 ± 0.424
0.628TyrHis: 0.628 ± 0.219
3.452TyrIle: 3.452 ± 0.528
4.786TyrLys: 4.786 ± 0.567
3.452TyrLeu: 3.452 ± 0.52
1.098TyrMet: 1.098 ± 0.261
2.118TyrAsn: 2.118 ± 0.407
0.785TyrPro: 0.785 ± 0.186
1.961TyrGln: 1.961 ± 0.339
1.491TyrArg: 1.491 ± 0.434
2.746TyrSer: 2.746 ± 0.46
2.04TyrThr: 2.04 ± 0.417
2.354TyrVal: 2.354 ± 0.451
0.314TyrTrp: 0.314 ± 0.171
1.569TyrTyr: 1.569 ± 0.493
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (12747 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski