Capybara microvirus Cap1_SP_109
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W453|A0A4P8W453_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_109 OX=2584807 PE=4 SV=1
MM1 pKa = 7.68 RR2 pKa = 11.84 FYY4 pKa = 11.54 VEE6 pKa = 4.44 GSRR9 pKa = 11.84 LFNRR13 pKa = 11.84 STNEE17 pKa = 3.4 DD18 pKa = 3.71 SIKK21 pKa = 10.31 VKK23 pKa = 10.67 SYY25 pKa = 10.35 VDD27 pKa = 3.74 VIEE30 pKa = 4.31 KK31 pKa = 10.09 VVNEE35 pKa = 3.87 KK36 pKa = 10.08 TGLIEE41 pKa = 4.01 EE42 pKa = 4.38 KK43 pKa = 10.51 VVCKK47 pKa = 10.37 EE48 pKa = 4.07 LTDD51 pKa = 3.71 DD52 pKa = 4.05 RR53 pKa = 11.84 YY54 pKa = 10.95 EE55 pKa = 4.34 GLSSYY60 pKa = 11.35 DD61 pKa = 3.14 FDD63 pKa = 5.93 LDD65 pKa = 4.27 HH66 pKa = 7.07 IVLAGATSLLQPCPKK81 pKa = 10.22 VSGNVFDD88 pKa = 5.39 ISDD91 pKa = 3.88 SLASNVDD98 pKa = 3.42 YY99 pKa = 11.4 LGDD102 pKa = 3.61 YY103 pKa = 11.35 YY104 pKa = 10.58 EE105 pKa = 4.23 TNKK108 pKa = 10.47 NIKK111 pKa = 9.6 EE112 pKa = 4.1 DD113 pKa = 3.63 EE114 pKa = 4.13
Molecular weight: 12.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.409
IPC2_protein 4.418
IPC_protein 4.342
Toseland 4.164
ProMoST 4.406
Dawson 4.291
Bjellqvist 4.495
Wikipedia 4.19
Rodwell 4.164
Grimsley 4.075
Solomon 4.291
Lehninger 4.253
Nozaki 4.406
DTASelect 4.596
Thurlkill 4.177
EMBOSS 4.202
Sillero 4.444
Patrickios 3.617
IPC_peptide 4.291
IPC2_peptide 4.431
IPC2.peptide.svr19 4.387
Protein with the highest isoelectric point:
>tr|A0A4P8W4Y4|A0A4P8W4Y4_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_109 OX=2584807 PE=4 SV=1
MM1 pKa = 7.57 CLSPIKK7 pKa = 10.15 IYY9 pKa = 10.65 NRR11 pKa = 11.84 SEE13 pKa = 4.1 SLVTNGPLYY22 pKa = 10.59 IEE24 pKa = 4.63 VPCGKK29 pKa = 10.13 CSEE32 pKa = 4.31 CQKK35 pKa = 10.71 QKK37 pKa = 9.31 HH38 pKa = 4.92 TDD40 pKa = 3.18 YY41 pKa = 10.83 CIRR44 pKa = 11.84 AFWQYY49 pKa = 11.33 QEE51 pKa = 4.13 TLIKK55 pKa = 10.55 GGYY58 pKa = 9.69 CYY60 pKa = 10.59 FDD62 pKa = 3.35 TLTYY66 pKa = 10.71 DD67 pKa = 3.45 SSHH70 pKa = 6.4 LPKK73 pKa = 10.19 FDD75 pKa = 5.58 GISCFDD81 pKa = 3.19 KK82 pKa = 10.56 THH84 pKa = 5.38 IQKK87 pKa = 10.74 FIRR90 pKa = 11.84 DD91 pKa = 3.64 LRR93 pKa = 11.84 RR94 pKa = 11.84 QLDD97 pKa = 3.45 YY98 pKa = 11.29 HH99 pKa = 6.7 FYY101 pKa = 9.51 EE102 pKa = 4.36 SKK104 pKa = 10.76 GRR106 pKa = 11.84 SGSIKK111 pKa = 10.57 EE112 pKa = 3.85 NFKK115 pKa = 11.19 YY116 pKa = 10.51 FLTSEE121 pKa = 4.44 YY122 pKa = 10.15 GGKK125 pKa = 8.68 RR126 pKa = 11.84 HH127 pKa = 6.13 RR128 pKa = 11.84 PHH130 pKa = 6.21 YY131 pKa = 10.12 HH132 pKa = 4.96 VLFFVTDD139 pKa = 3.75 PSISVSQLKK148 pKa = 10.53 YY149 pKa = 10.75 YY150 pKa = 10.19 INKK153 pKa = 8.55 CWIYY157 pKa = 10.56 GITDD161 pKa = 3.68 SVSTTNDD168 pKa = 2.61 RR169 pKa = 11.84 VVRR172 pKa = 11.84 GISAISYY179 pKa = 5.31 VTKK182 pKa = 10.82 YY183 pKa = 7.22 VTKK186 pKa = 10.33 DD187 pKa = 3.09 QEE189 pKa = 4.05 FSLLIEE195 pKa = 4.58 KK196 pKa = 10.12 RR197 pKa = 11.84 LKK199 pKa = 10.66 YY200 pKa = 10.79 YY201 pKa = 10.71 SILYY205 pKa = 10.43 GSDD208 pKa = 2.77 SLEE211 pKa = 3.88 YY212 pKa = 10.56 LEE214 pKa = 5.54 KK215 pKa = 11.03 VKK217 pKa = 10.44 DD218 pKa = 3.81 YY219 pKa = 11.4 KK220 pKa = 10.63 RR221 pKa = 11.84 FRR223 pKa = 11.84 PFFNSSTNFGSSALKK238 pKa = 10.13 MVDD241 pKa = 3.65 QDD243 pKa = 4.16 LLLDD247 pKa = 3.65 GRR249 pKa = 11.84 IWISDD254 pKa = 3.36 SKK256 pKa = 9.52 TSKK259 pKa = 10.69 KK260 pKa = 10.13 FVSLPLYY267 pKa = 9.7 YY268 pKa = 9.77 RR269 pKa = 11.84 RR270 pKa = 11.84 KK271 pKa = 10.25 LFYY274 pKa = 10.74 DD275 pKa = 3.93 VIKK278 pKa = 10.3 TEE280 pKa = 4.34 DD281 pKa = 3.65 GRR283 pKa = 11.84 IKK285 pKa = 10.27 WVMNDD290 pKa = 2.75 RR291 pKa = 11.84 GFDD294 pKa = 3.68 YY295 pKa = 10.68 KK296 pKa = 10.92 LKK298 pKa = 10.81 RR299 pKa = 11.84 LDD301 pKa = 3.66 TNISLLEE308 pKa = 4.08 DD309 pKa = 4.28 RR310 pKa = 11.84 YY311 pKa = 9.49 NTYY314 pKa = 11.01 YY315 pKa = 11.33 LNLDD319 pKa = 3.76 NYY321 pKa = 10.44 CSEE324 pKa = 5.03 DD325 pKa = 4.25 YY326 pKa = 10.74 FPKK329 pKa = 10.42 DD330 pKa = 3.3 YY331 pKa = 10.92 VSRR334 pKa = 11.84 LILSYY339 pKa = 11.34 LDD341 pKa = 3.16 GRR343 pKa = 11.84 TFKK346 pKa = 11.09 DD347 pKa = 3.4 FATYY351 pKa = 10.25 LYY353 pKa = 10.3 VYY355 pKa = 8.95 RR356 pKa = 11.84 GKK358 pKa = 9.53 MFSGGVLPDD367 pKa = 3.06 FHH369 pKa = 8.48 KK370 pKa = 10.57 FYY372 pKa = 11.39 SMSLRR377 pKa = 11.84 GSTIFSKK384 pKa = 10.68 LYY386 pKa = 10.43 SSNLSIRR393 pKa = 11.84 SRR395 pKa = 11.84 MRR397 pKa = 11.84 SRR399 pKa = 11.84 LDD401 pKa = 3.4 KK402 pKa = 10.79 ISIRR406 pKa = 11.84 EE407 pKa = 3.83 TSFSSFRR414 pKa = 11.84 NFDD417 pKa = 3.12 KK418 pKa = 10.91 LYY420 pKa = 10.84 NLFNVLLRR428 pKa = 11.84 EE429 pKa = 4.29 SHH431 pKa = 6.69 TSNQYY436 pKa = 8.9 TFDD439 pKa = 3.85 YY440 pKa = 11.05 KK441 pKa = 11.02 EE442 pKa = 4.17 DD443 pKa = 4.52 LIGKK447 pKa = 9.14 YY448 pKa = 10.35 KK449 pKa = 10.97 LILL452 pKa = 3.89
Molecular weight: 53.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.715
IPC2_protein 8.814
IPC_protein 8.682
Toseland 9.092
ProMoST 9.063
Dawson 9.472
Bjellqvist 9.311
Wikipedia 9.692
Rodwell 9.604
Grimsley 9.575
Solomon 9.502
Lehninger 9.443
Nozaki 9.282
DTASelect 9.253
Thurlkill 9.297
EMBOSS 9.575
Sillero 9.458
Patrickios 4.05
IPC_peptide 9.487
IPC2_peptide 8.039
IPC2.peptide.svr19 7.784
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1743
114
570
348.6
39.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.475 ± 1.436
0.975 ± 0.361
6.942 ± 0.562
5.049 ± 0.717
4.877 ± 0.625
6.368 ± 0.855
1.205 ± 0.237
5.393 ± 0.389
6.942 ± 0.914
9.122 ± 1.145
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.721 ± 0.335
5.967 ± 1.06
2.582 ± 0.614
3.442 ± 0.954
5.278 ± 0.531
9.237 ± 0.588
6.311 ± 0.435
6.713 ± 0.916
0.688 ± 0.128
6.713 ± 1.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here