Amino acid dipepetide frequency for Mycoplasma pulmonis (strain UAB CTIP)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.43AlaAla: 2.43 ± 0.15
0.166AlaCys: 0.166 ± 0.022
1.661AlaAsp: 1.661 ± 0.071
2.018AlaGlu: 2.018 ± 0.104
2.835AlaPhe: 2.835 ± 0.112
2.167AlaGly: 2.167 ± 0.113
0.793AlaHis: 0.793 ± 0.051
4.911AlaIle: 4.911 ± 0.147
5.178AlaLys: 5.178 ± 0.171
5.223AlaLeu: 5.223 ± 0.16
0.865AlaMet: 0.865 ± 0.062
3.555AlaAsn: 3.555 ± 0.168
1.429AlaPro: 1.429 ± 0.171
1.983AlaGln: 1.983 ± 0.103
1.682AlaArg: 1.682 ± 0.081
3.776AlaSer: 3.776 ± 0.138
2.295AlaThr: 2.295 ± 0.113
1.845AlaVal: 1.845 ± 0.099
0.357AlaTrp: 0.357 ± 0.039
1.769AlaTyr: 1.769 ± 0.084
0.0AlaXaa: 0.0 ± 0.0
Cys
0.145CysAla: 0.145 ± 0.027
0.014CysCys: 0.014 ± 0.007
0.194CysAsp: 0.194 ± 0.025
0.156CysGlu: 0.156 ± 0.025
0.287CysPhe: 0.287 ± 0.033
0.166CysGly: 0.166 ± 0.026
0.093CysHis: 0.093 ± 0.018
0.256CysIle: 0.256 ± 0.033
0.256CysLys: 0.256 ± 0.033
0.26CysLeu: 0.26 ± 0.031
0.017CysMet: 0.017 ± 0.007
0.149CysAsn: 0.149 ± 0.025
0.142CysPro: 0.142 ± 0.023
0.149CysGln: 0.149 ± 0.022
0.059CysArg: 0.059 ± 0.014
0.336CysSer: 0.336 ± 0.038
0.107CysThr: 0.107 ± 0.02
0.121CysVal: 0.121 ± 0.02
0.017CysTrp: 0.017 ± 0.008
0.093CysTyr: 0.093 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
2.167AspAla: 2.167 ± 0.118
0.128AspCys: 0.128 ± 0.023
2.675AspAsp: 2.675 ± 0.101
4.264AspGlu: 4.264 ± 0.152
4.545AspPhe: 4.545 ± 0.129
2.426AspGly: 2.426 ± 0.148
0.862AspHis: 0.862 ± 0.053
4.32AspIle: 4.32 ± 0.128
5.756AspLys: 5.756 ± 0.156
5.971AspLeu: 5.971 ± 0.169
0.55AspMet: 0.55 ± 0.047
3.146AspAsn: 3.146 ± 0.157
1.637AspPro: 1.637 ± 0.083
2.696AspGln: 2.696 ± 0.136
1.166AspArg: 1.166 ± 0.066
3.572AspSer: 3.572 ± 0.112
2.028AspThr: 2.028 ± 0.208
2.655AspVal: 2.655 ± 0.087
0.474AspTrp: 0.474 ± 0.041
2.485AspTyr: 2.485 ± 0.091
0.0AspXaa: 0.0 ± 0.0
Glu
3.063GluAla: 3.063 ± 0.149
0.159GluCys: 0.159 ± 0.022
3.281GluAsp: 3.281 ± 0.095
4.846GluGlu: 4.846 ± 0.187
3.707GluPhe: 3.707 ± 0.124
2.395GluGly: 2.395 ± 0.118
0.931GluHis: 0.931 ± 0.06
7.746GluIle: 7.746 ± 0.184
9.913GluLys: 9.913 ± 0.217
6.047GluLeu: 6.047 ± 0.143
1.076GluMet: 1.076 ± 0.074
7.005GluAsn: 7.005 ± 0.185
1.315GluPro: 1.315 ± 0.089
2.762GluGln: 2.762 ± 0.11
1.72GluArg: 1.72 ± 0.101
3.939GluSer: 3.939 ± 0.131
2.534GluThr: 2.534 ± 0.113
3.613GluVal: 3.613 ± 0.117
0.405GluTrp: 0.405 ± 0.04
2.492GluTyr: 2.492 ± 0.091
0.0GluXaa: 0.0 ± 0.0
Phe
2.703PheAla: 2.703 ± 0.116
0.305PheCys: 0.305 ± 0.029
3.96PheAsp: 3.96 ± 0.11
4.783PheGlu: 4.783 ± 0.157
4.126PhePhe: 4.126 ± 0.167
2.579PheGly: 2.579 ± 0.094
0.696PheHis: 0.696 ± 0.046
5.043PheIle: 5.043 ± 0.184
5.849PheLys: 5.849 ± 0.176
6.964PheLeu: 6.964 ± 0.2
0.879PheMet: 0.879 ± 0.062
3.928PheAsn: 3.928 ± 0.121
1.194PhePro: 1.194 ± 0.064
1.814PheGln: 1.814 ± 0.091
1.603PheArg: 1.603 ± 0.075
5.275PheSer: 5.275 ± 0.159
2.361PheThr: 2.361 ± 0.091
3.742PheVal: 3.742 ± 0.121
0.758PheTrp: 0.758 ± 0.053
2.485PheTyr: 2.485 ± 0.118
0.0PheXaa: 0.0 ± 0.0
Gly
2.246GlyAla: 2.246 ± 0.094
0.125GlyCys: 0.125 ± 0.025
2.416GlyAsp: 2.416 ± 0.238
2.409GlyGlu: 2.409 ± 0.11
3.049GlyPhe: 3.049 ± 0.114
2.655GlyGly: 2.655 ± 0.22
0.772GlyHis: 0.772 ± 0.062
4.572GlyIle: 4.572 ± 0.154
4.558GlyLys: 4.558 ± 0.14
3.793GlyLeu: 3.793 ± 0.139
0.689GlyMet: 0.689 ± 0.049
2.534GlyAsn: 2.534 ± 0.3
0.962GlyPro: 0.962 ± 0.067
1.952GlyGln: 1.952 ± 0.134
1.402GlyArg: 1.402 ± 0.084
3.617GlySer: 3.617 ± 0.206
2.309GlyThr: 2.309 ± 0.109
2.537GlyVal: 2.537 ± 0.125
0.474GlyTrp: 0.474 ± 0.043
1.917GlyTyr: 1.917 ± 0.076
0.0GlyXaa: 0.0 ± 0.0
His
0.588HisAla: 0.588 ± 0.049
0.059HisCys: 0.059 ± 0.013
0.744HisAsp: 0.744 ± 0.055
0.966HisGlu: 0.966 ± 0.055
1.056HisPhe: 1.056 ± 0.069
0.786HisGly: 0.786 ± 0.054
0.273HisHis: 0.273 ± 0.03
1.201HisIle: 1.201 ± 0.067
1.277HisLys: 1.277 ± 0.077
1.554HisLeu: 1.554 ± 0.077
0.215HisMet: 0.215 ± 0.025
0.865HisAsn: 0.865 ± 0.05
0.502HisPro: 0.502 ± 0.049
0.457HisGln: 0.457 ± 0.042
0.467HisArg: 0.467 ± 0.046
1.097HisSer: 1.097 ± 0.064
0.571HisThr: 0.571 ± 0.053
0.755HisVal: 0.755 ± 0.048
0.125HisTrp: 0.125 ± 0.021
0.564HisTyr: 0.564 ± 0.045
0.0HisXaa: 0.0 ± 0.0
Ile
5.057IleAla: 5.057 ± 0.133
0.346IleCys: 0.346 ± 0.036
5.794IleAsp: 5.794 ± 0.131
6.635IleGlu: 6.635 ± 0.189
6.334IlePhe: 6.334 ± 0.205
4.032IleGly: 4.032 ± 0.145
1.263IleHis: 1.263 ± 0.064
8.269IleIle: 8.269 ± 0.225
9.48IleLys: 9.48 ± 0.193
8.961IleLeu: 8.961 ± 0.237
1.519IleMet: 1.519 ± 0.078
6.936IleAsn: 6.936 ± 0.183
2.617IlePro: 2.617 ± 0.094
2.952IleGln: 2.952 ± 0.097
2.433IleArg: 2.433 ± 0.088
7.85IleSer: 7.85 ± 0.195
4.365IleThr: 4.365 ± 0.137
5.534IleVal: 5.534 ± 0.162
0.848IleTrp: 0.848 ± 0.06
3.589IleTyr: 3.589 ± 0.125
0.0IleXaa: 0.0 ± 0.0
Lys
4.977LysAla: 4.977 ± 0.141
0.215LysCys: 0.215 ± 0.028
6.656LysAsp: 6.656 ± 0.199
8.494LysGlu: 8.494 ± 0.2
4.925LysPhe: 4.925 ± 0.144
3.773LysGly: 3.773 ± 0.122
1.468LysHis: 1.468 ± 0.074
12.249LysIle: 12.249 ± 0.27
12.01LysLys: 12.01 ± 0.294
8.739LysLeu: 8.739 ± 0.196
2.329LysMet: 2.329 ± 0.096
11.007LysAsn: 11.007 ± 0.275
2.464LysPro: 2.464 ± 0.147
3.568LysGln: 3.568 ± 0.107
3.17LysArg: 3.17 ± 0.109
6.559LysSer: 6.559 ± 0.16
6.144LysThr: 6.144 ± 0.148
5.995LysVal: 5.995 ± 0.17
1.038LysTrp: 1.038 ± 0.06
4.24LysTyr: 4.24 ± 0.141
0.0LysXaa: 0.0 ± 0.0
Leu
5.036LeuAla: 5.036 ± 0.147
0.249LeuCys: 0.249 ± 0.032
5.6LeuAsp: 5.6 ± 0.151
7.428LeuGlu: 7.428 ± 0.201
5.074LeuPhe: 5.074 ± 0.164
4.34LeuGly: 4.34 ± 0.138
1.07LeuHis: 1.07 ± 0.067
9.314LeuIle: 9.314 ± 0.196
11.349LeuLys: 11.349 ± 0.239
8.251LeuLeu: 8.251 ± 0.21
1.429LeuMet: 1.429 ± 0.065
7.019LeuAsn: 7.019 ± 0.183
2.589LeuPro: 2.589 ± 0.109
2.554LeuGln: 2.554 ± 0.099
2.738LeuArg: 2.738 ± 0.093
7.549LeuSer: 7.549 ± 0.186
4.645LeuThr: 4.645 ± 0.156
5.784LeuVal: 5.784 ± 0.172
0.865LeuTrp: 0.865 ± 0.057
2.831LeuTyr: 2.831 ± 0.114
0.0LeuXaa: 0.0 ± 0.0
Met
1.076MetAla: 1.076 ± 0.079
0.042MetCys: 0.042 ± 0.013
0.893MetAsp: 0.893 ± 0.106
0.917MetGlu: 0.917 ± 0.064
0.9MetPhe: 0.9 ± 0.058
0.758MetGly: 0.758 ± 0.099
0.222MetHis: 0.222 ± 0.028
1.412MetIle: 1.412 ± 0.085
1.838MetLys: 1.838 ± 0.086
1.502MetLeu: 1.502 ± 0.077
0.301MetMet: 0.301 ± 0.03
1.142MetAsn: 1.142 ± 0.059
0.578MetPro: 0.578 ± 0.043
0.488MetGln: 0.488 ± 0.045
0.419MetArg: 0.419 ± 0.041
1.132MetSer: 1.132 ± 0.062
0.903MetThr: 0.903 ± 0.151
0.921MetVal: 0.921 ± 0.088
0.114MetTrp: 0.114 ± 0.018
0.401MetTyr: 0.401 ± 0.039
0.0MetXaa: 0.0 ± 0.0
Asn
2.883AsnAla: 2.883 ± 0.136
0.177AsnCys: 0.177 ± 0.028
4.261AsnAsp: 4.261 ± 0.162
5.216AsnGlu: 5.216 ± 0.146
5.251AsnPhe: 5.251 ± 0.18
3.184AsnGly: 3.184 ± 0.151
1.149AsnHis: 1.149 ± 0.066
6.652AsnIle: 6.652 ± 0.172
8.123AsnLys: 8.123 ± 0.227
8.148AsnLeu: 8.148 ± 0.206
1.059AsnMet: 1.059 ± 0.054
5.559AsnAsn: 5.559 ± 0.19
3.056AsnPro: 3.056 ± 0.231
3.298AsnGln: 3.298 ± 0.153
1.651AsnArg: 1.651 ± 0.067
5.77AsnSer: 5.77 ± 0.175
3.295AsnThr: 3.295 ± 0.234
3.631AsnVal: 3.631 ± 0.102
0.81AsnTrp: 0.81 ± 0.054
3.022AsnTyr: 3.022 ± 0.12
0.0AsnXaa: 0.0 ± 0.0
Pro
1.301ProAla: 1.301 ± 0.208
0.087ProCys: 0.087 ± 0.019
1.073ProAsp: 1.073 ± 0.072
2.097ProGlu: 2.097 ± 0.125
1.474ProPhe: 1.474 ± 0.084
1.409ProGly: 1.409 ± 0.176
0.363ProHis: 0.363 ± 0.039
2.444ProIle: 2.444 ± 0.101
2.762ProLys: 2.762 ± 0.148
2.367ProLeu: 2.367 ± 0.098
0.405ProMet: 0.405 ± 0.041
2.039ProAsn: 2.039 ± 0.093
0.834ProPro: 0.834 ± 0.208
1.018ProGln: 1.018 ± 0.141
0.779ProArg: 0.779 ± 0.06
2.672ProSer: 2.672 ± 0.212
1.443ProThr: 1.443 ± 0.093
1.232ProVal: 1.232 ± 0.07
0.308ProTrp: 0.308 ± 0.037
0.962ProTyr: 0.962 ± 0.063
0.0ProXaa: 0.0 ± 0.0
Gln
1.845GlnAla: 1.845 ± 0.089
0.073GlnCys: 0.073 ± 0.015
1.893GlnAsp: 1.893 ± 0.085
2.99GlnGlu: 2.99 ± 0.128
1.267GlnPhe: 1.267 ± 0.069
1.834GlnGly: 1.834 ± 0.199
0.388GlnHis: 0.388 ± 0.039
3.742GlnIle: 3.742 ± 0.137
4.922GlnLys: 4.922 ± 0.149
2.561GlnLeu: 2.561 ± 0.096
0.824GlnMet: 0.824 ± 0.189
3.593GlnAsn: 3.593 ± 0.146
0.658GlnPro: 0.658 ± 0.064
1.132GlnGln: 1.132 ± 0.087
1.478GlnArg: 1.478 ± 0.068
2.637GlnSer: 2.637 ± 0.107
1.796GlnThr: 1.796 ± 0.208
1.734GlnVal: 1.734 ± 0.085
0.28GlnTrp: 0.28 ± 0.033
1.118GlnTyr: 1.118 ± 0.059
0.0GlnXaa: 0.0 ± 0.0
Arg
1.533ArgAla: 1.533 ± 0.088
0.069ArgCys: 0.069 ± 0.017
1.474ArgAsp: 1.474 ± 0.072
1.959ArgGlu: 1.959 ± 0.098
1.623ArgPhe: 1.623 ± 0.081
1.426ArgGly: 1.426 ± 0.078
0.408ArgHis: 0.408 ± 0.042
2.606ArgIle: 2.606 ± 0.098
3.288ArgLys: 3.288 ± 0.106
2.451ArgLeu: 2.451 ± 0.096
0.536ArgMet: 0.536 ± 0.042
1.942ArgAsn: 1.942 ± 0.081
0.727ArgPro: 0.727 ± 0.057
1.201ArgGln: 1.201 ± 0.07
1.121ArgArg: 1.121 ± 0.068
1.8ArgSer: 1.8 ± 0.085
1.184ArgThr: 1.184 ± 0.078
1.599ArgVal: 1.599 ± 0.08
0.294ArgTrp: 0.294 ± 0.036
1.038ArgTyr: 1.038 ± 0.062
0.0ArgXaa: 0.0 ± 0.0
Ser
3.243SerAla: 3.243 ± 0.113
0.401SerCys: 0.401 ± 0.04
3.513SerAsp: 3.513 ± 0.135
4.486SerGlu: 4.486 ± 0.144
4.925SerPhe: 4.925 ± 0.147
3.596SerGly: 3.596 ± 0.161
1.139SerHis: 1.139 ± 0.065
6.881SerIle: 6.881 ± 0.188
8.009SerLys: 8.009 ± 0.165
8.227SerLeu: 8.227 ± 0.184
1.135SerMet: 1.135 ± 0.064
5.129SerAsn: 5.129 ± 0.148
1.879SerPro: 1.879 ± 0.077
3.444SerGln: 3.444 ± 0.235
2.205SerArg: 2.205 ± 0.087
6.531SerSer: 6.531 ± 0.196
3.811SerThr: 3.811 ± 0.23
3.717SerVal: 3.717 ± 0.127
0.91SerTrp: 0.91 ± 0.057
2.835SerTyr: 2.835 ± 0.121
0.0SerXaa: 0.0 ± 0.0
Thr
1.499ThrAla: 1.499 ± 0.092
0.125ThrCys: 0.125 ± 0.027
1.551ThrAsp: 1.551 ± 0.084
1.762ThrGlu: 1.762 ± 0.093
2.9ThrPhe: 2.9 ± 0.103
2.513ThrGly: 2.513 ± 0.204
0.841ThrHis: 0.841 ± 0.059
4.562ThrIle: 4.562 ± 0.138
5.576ThrLys: 5.576 ± 0.165
4.891ThrLeu: 4.891 ± 0.135
0.845ThrMet: 0.845 ± 0.193
4.056ThrAsn: 4.056 ± 0.239
1.755ThrPro: 1.755 ± 0.235
1.727ThrGln: 1.727 ± 0.093
1.499ThrArg: 1.499 ± 0.08
4.05ThrSer: 4.05 ± 0.216
2.932ThrThr: 2.932 ± 0.213
1.551ThrVal: 1.551 ± 0.079
0.422ThrTrp: 0.422 ± 0.043
1.613ThrTyr: 1.613 ± 0.087
0.0ThrXaa: 0.0 ± 0.0
Val
2.99ValAla: 2.99 ± 0.11
0.142ValCys: 0.142 ± 0.025
3.395ValAsp: 3.395 ± 0.106
4.136ValGlu: 4.136 ± 0.142
3.582ValPhe: 3.582 ± 0.136
2.793ValGly: 2.793 ± 0.13
0.796ValHis: 0.796 ± 0.059
4.676ValIle: 4.676 ± 0.125
5.053ValLys: 5.053 ± 0.165
5.022ValLeu: 5.022 ± 0.163
0.696ValMet: 0.696 ± 0.052
3.34ValAsn: 3.34 ± 0.117
1.464ValPro: 1.464 ± 0.077
1.523ValGln: 1.523 ± 0.072
1.26ValArg: 1.26 ± 0.069
4.233ValSer: 4.233 ± 0.121
2.004ValThr: 2.004 ± 0.116
3.496ValVal: 3.496 ± 0.119
0.353ValTrp: 0.353 ± 0.036
2.052ValTyr: 2.052 ± 0.09
0.0ValXaa: 0.0 ± 0.0
Trp
0.412TrpAla: 0.412 ± 0.04
0.028TrpCys: 0.028 ± 0.01
0.44TrpAsp: 0.44 ± 0.038
0.602TrpGlu: 0.602 ± 0.042
0.585TrpPhe: 0.585 ± 0.053
0.346TrpGly: 0.346 ± 0.036
0.135TrpHis: 0.135 ± 0.019
0.976TrpIle: 0.976 ± 0.066
1.101TrpLys: 1.101 ± 0.065
0.845TrpLeu: 0.845 ± 0.064
0.156TrpMet: 0.156 ± 0.024
0.758TrpAsn: 0.758 ± 0.056
0.27TrpPro: 0.27 ± 0.029
0.35TrpGln: 0.35 ± 0.044
0.305TrpArg: 0.305 ± 0.034
0.678TrpSer: 0.678 ± 0.048
0.481TrpThr: 0.481 ± 0.044
0.498TrpVal: 0.498 ± 0.042
0.118TrpTrp: 0.118 ± 0.021
0.391TrpTyr: 0.391 ± 0.038
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.741TyrAla: 1.741 ± 0.086
0.111TyrCys: 0.111 ± 0.02
2.049TyrAsp: 2.049 ± 0.078
2.786TyrGlu: 2.786 ± 0.108
2.599TyrPhe: 2.599 ± 0.094
1.81TyrGly: 1.81 ± 0.098
0.46TyrHis: 0.46 ± 0.036
2.984TyrIle: 2.984 ± 0.104
3.724TyrLys: 3.724 ± 0.133
3.939TyrLeu: 3.939 ± 0.121
0.429TyrMet: 0.429 ± 0.039
2.492TyrAsn: 2.492 ± 0.107
0.997TyrPro: 0.997 ± 0.052
1.696TyrGln: 1.696 ± 0.075
1.125TyrArg: 1.125 ± 0.069
2.945TyrSer: 2.945 ± 0.089
1.461TyrThr: 1.461 ± 0.086
2.049TyrVal: 2.049 ± 0.083
0.502TyrTrp: 0.502 ± 0.044
1.533TyrTyr: 1.533 ± 0.081
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 778 proteins (288921 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski