Amino acid dipepetide frequency for Halorubrum phage CGphi46

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.623AlaAla: 9.623 ± 1.162
0.576AlaCys: 0.576 ± 0.202
9.459AlaAsp: 9.459 ± 0.905
9.706AlaGlu: 9.706 ± 0.993
2.632AlaPhe: 2.632 ± 0.44
9.541AlaGly: 9.541 ± 0.874
1.81AlaHis: 1.81 ± 0.457
4.771AlaIle: 4.771 ± 0.768
2.55AlaLys: 2.55 ± 0.595
7.403AlaLeu: 7.403 ± 1.023
2.221AlaMet: 2.221 ± 0.516
2.221AlaAsn: 2.221 ± 0.401
4.771AlaPro: 4.771 ± 0.589
2.221AlaGln: 2.221 ± 0.39
6.827AlaArg: 6.827 ± 0.809
5.1AlaSer: 5.1 ± 0.708
7.156AlaThr: 7.156 ± 0.838
8.39AlaVal: 8.39 ± 1.144
1.316AlaTrp: 1.316 ± 0.31
2.139AlaTyr: 2.139 ± 0.406
0.0AlaXaa: 0.0 ± 0.0
Cys
0.576CysAla: 0.576 ± 0.249
0.082CysCys: 0.082 ± 0.103
0.658CysAsp: 0.658 ± 0.244
0.494CysGlu: 0.494 ± 0.235
0.082CysPhe: 0.082 ± 0.077
0.74CysGly: 0.74 ± 0.335
0.082CysHis: 0.082 ± 0.082
0.247CysIle: 0.247 ± 0.15
0.247CysLys: 0.247 ± 0.148
0.329CysLeu: 0.329 ± 0.164
0.0CysMet: 0.0 ± 0.0
0.165CysAsn: 0.165 ± 0.123
0.165CysPro: 0.165 ± 0.159
0.411CysGln: 0.411 ± 0.199
0.411CysArg: 0.411 ± 0.214
0.494CysSer: 0.494 ± 0.201
0.165CysThr: 0.165 ± 0.115
0.247CysVal: 0.247 ± 0.166
0.082CysTrp: 0.082 ± 0.103
0.247CysTyr: 0.247 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
11.844AspAla: 11.844 ± 0.88
0.247AspCys: 0.247 ± 0.148
9.294AspAsp: 9.294 ± 0.981
9.212AspGlu: 9.212 ± 0.948
1.481AspPhe: 1.481 ± 0.322
9.623AspGly: 9.623 ± 0.779
2.056AspHis: 2.056 ± 0.452
2.55AspIle: 2.55 ± 0.474
1.316AspLys: 1.316 ± 0.348
7.814AspLeu: 7.814 ± 0.846
1.398AspMet: 1.398 ± 0.33
1.81AspAsn: 1.81 ± 0.518
4.277AspPro: 4.277 ± 0.599
1.727AspGln: 1.727 ± 0.381
5.593AspArg: 5.593 ± 0.819
4.688AspSer: 4.688 ± 0.817
7.567AspThr: 7.567 ± 0.987
8.636AspVal: 8.636 ± 0.705
1.316AspTrp: 1.316 ± 0.292
2.385AspTyr: 2.385 ± 0.386
0.0AspXaa: 0.0 ± 0.0
Glu
9.706GluAla: 9.706 ± 0.887
0.576GluCys: 0.576 ± 0.243
5.758GluAsp: 5.758 ± 0.789
5.264GluGlu: 5.264 ± 0.951
2.303GluPhe: 2.303 ± 0.513
6.58GluGly: 6.58 ± 0.621
2.468GluHis: 2.468 ± 0.486
4.524GluIle: 4.524 ± 0.462
1.892GluLys: 1.892 ± 0.509
7.074GluLeu: 7.074 ± 0.666
2.468GluMet: 2.468 ± 0.536
2.385GluAsn: 2.385 ± 0.365
4.277GluPro: 4.277 ± 0.733
4.03GluGln: 4.03 ± 0.566
7.978GluArg: 7.978 ± 0.985
4.771GluSer: 4.771 ± 0.582
5.922GluThr: 5.922 ± 0.683
5.922GluVal: 5.922 ± 0.707
2.797GluTrp: 2.797 ± 0.485
2.714GluTyr: 2.714 ± 0.437
0.0GluXaa: 0.0 ± 0.0
Phe
2.714PheAla: 2.714 ± 0.458
0.082PheCys: 0.082 ± 0.091
2.797PheAsp: 2.797 ± 0.411
4.03PheGlu: 4.03 ± 0.492
0.658PhePhe: 0.658 ± 0.248
2.468PheGly: 2.468 ± 0.474
0.247PheHis: 0.247 ± 0.171
0.987PheIle: 0.987 ± 0.384
0.74PheLys: 0.74 ± 0.266
1.81PheLeu: 1.81 ± 0.397
0.247PheMet: 0.247 ± 0.148
0.74PheAsn: 0.74 ± 0.215
0.411PhePro: 0.411 ± 0.2
0.658PheGln: 0.658 ± 0.204
1.727PheArg: 1.727 ± 0.309
1.563PheSer: 1.563 ± 0.3
1.069PheThr: 1.069 ± 0.232
2.056PheVal: 2.056 ± 0.379
0.494PheTrp: 0.494 ± 0.215
0.494PheTyr: 0.494 ± 0.233
0.0PheXaa: 0.0 ± 0.0
Gly
8.061GlyAla: 8.061 ± 0.963
0.823GlyCys: 0.823 ± 0.258
8.883GlyAsp: 8.883 ± 0.934
7.814GlyGlu: 7.814 ± 0.764
2.714GlyPhe: 2.714 ± 0.593
10.693GlyGly: 10.693 ± 2.725
1.645GlyHis: 1.645 ± 0.368
2.55GlyIle: 2.55 ± 0.506
0.987GlyLys: 0.987 ± 0.238
5.182GlyLeu: 5.182 ± 0.596
1.563GlyMet: 1.563 ± 0.413
1.481GlyAsn: 1.481 ± 0.359
2.714GlyPro: 2.714 ± 0.514
2.797GlyGln: 2.797 ± 0.44
4.771GlyArg: 4.771 ± 0.748
6.745GlySer: 6.745 ± 0.841
4.935GlyThr: 4.935 ± 0.572
7.485GlyVal: 7.485 ± 0.757
1.563GlyTrp: 1.563 ± 0.298
2.797GlyTyr: 2.797 ± 0.475
0.0GlyXaa: 0.0 ± 0.0
His
2.385HisAla: 2.385 ± 0.484
0.165HisCys: 0.165 ± 0.135
1.563HisAsp: 1.563 ± 0.372
1.481HisGlu: 1.481 ± 0.37
0.576HisPhe: 0.576 ± 0.193
1.645HisGly: 1.645 ± 0.46
0.494HisHis: 0.494 ± 0.226
0.658HisIle: 0.658 ± 0.257
0.411HisLys: 0.411 ± 0.155
1.974HisLeu: 1.974 ± 0.467
0.247HisMet: 0.247 ± 0.142
1.316HisAsn: 1.316 ± 0.412
1.234HisPro: 1.234 ± 0.284
0.74HisGln: 0.74 ± 0.265
1.152HisArg: 1.152 ± 0.377
0.823HisSer: 0.823 ± 0.325
1.234HisThr: 1.234 ± 0.288
1.563HisVal: 1.563 ± 0.3
0.247HisTrp: 0.247 ± 0.144
0.905HisTyr: 0.905 ± 0.283
0.0HisXaa: 0.0 ± 0.0
Ile
3.784IleAla: 3.784 ± 0.548
0.0IleCys: 0.0 ± 0.0
3.537IleAsp: 3.537 ± 0.555
5.264IleGlu: 5.264 ± 0.668
0.411IlePhe: 0.411 ± 0.19
2.879IleGly: 2.879 ± 0.48
0.987IleHis: 0.987 ± 0.352
1.234IleIle: 1.234 ± 0.377
0.494IleLys: 0.494 ± 0.168
2.139IleLeu: 2.139 ± 0.388
0.576IleMet: 0.576 ± 0.206
1.152IleAsn: 1.152 ± 0.368
2.632IlePro: 2.632 ± 0.45
2.139IleGln: 2.139 ± 0.583
3.208IleArg: 3.208 ± 0.42
1.316IleSer: 1.316 ± 0.377
2.303IleThr: 2.303 ± 0.406
2.632IleVal: 2.632 ± 0.446
0.411IleTrp: 0.411 ± 0.178
0.658IleTyr: 0.658 ± 0.273
0.0IleXaa: 0.0 ± 0.0
Lys
2.632LysAla: 2.632 ± 0.453
0.0LysCys: 0.0 ± 0.0
1.645LysAsp: 1.645 ± 0.311
1.81LysGlu: 1.81 ± 0.448
0.74LysPhe: 0.74 ± 0.248
1.316LysGly: 1.316 ± 0.341
0.658LysHis: 0.658 ± 0.206
0.987LysIle: 0.987 ± 0.33
0.74LysLys: 0.74 ± 0.266
1.069LysLeu: 1.069 ± 0.354
0.329LysMet: 0.329 ± 0.146
0.658LysAsn: 0.658 ± 0.209
0.905LysPro: 0.905 ± 0.253
0.823LysGln: 0.823 ± 0.273
1.974LysArg: 1.974 ± 0.488
1.316LysSer: 1.316 ± 0.383
1.234LysThr: 1.234 ± 0.283
0.987LysVal: 0.987 ± 0.262
0.247LysTrp: 0.247 ± 0.142
0.658LysTyr: 0.658 ± 0.226
0.0LysXaa: 0.0 ± 0.0
Leu
7.567LeuAla: 7.567 ± 0.919
0.411LeuCys: 0.411 ± 0.204
7.814LeuAsp: 7.814 ± 0.764
6.498LeuGlu: 6.498 ± 0.886
1.81LeuPhe: 1.81 ± 0.468
5.429LeuGly: 5.429 ± 0.765
1.152LeuHis: 1.152 ± 0.263
1.892LeuIle: 1.892 ± 0.329
1.481LeuLys: 1.481 ± 0.322
4.442LeuLeu: 4.442 ± 0.723
1.234LeuMet: 1.234 ± 0.334
1.974LeuAsn: 1.974 ± 0.443
4.277LeuPro: 4.277 ± 0.617
2.139LeuGln: 2.139 ± 0.382
4.195LeuArg: 4.195 ± 0.554
5.1LeuSer: 5.1 ± 0.686
5.758LeuThr: 5.758 ± 0.814
4.442LeuVal: 4.442 ± 0.623
0.905LeuTrp: 0.905 ± 0.233
2.221LeuTyr: 2.221 ± 0.528
0.0LeuXaa: 0.0 ± 0.0
Met
1.398MetAla: 1.398 ± 0.389
0.165MetCys: 0.165 ± 0.115
0.823MetAsp: 0.823 ± 0.249
0.74MetGlu: 0.74 ± 0.242
0.329MetPhe: 0.329 ± 0.144
1.316MetGly: 1.316 ± 0.337
0.329MetHis: 0.329 ± 0.178
0.329MetIle: 0.329 ± 0.154
0.329MetLys: 0.329 ± 0.195
0.987MetLeu: 0.987 ± 0.284
0.329MetMet: 0.329 ± 0.174
0.494MetAsn: 0.494 ± 0.199
1.398MetPro: 1.398 ± 0.339
0.165MetGln: 0.165 ± 0.117
1.645MetArg: 1.645 ± 0.293
2.714MetSer: 2.714 ± 0.502
1.316MetThr: 1.316 ± 0.32
0.74MetVal: 0.74 ± 0.239
0.411MetTrp: 0.411 ± 0.153
0.165MetTyr: 0.165 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
3.043AsnAla: 3.043 ± 0.528
0.329AsnCys: 0.329 ± 0.182
2.139AsnAsp: 2.139 ± 0.517
2.221AsnGlu: 2.221 ± 0.47
0.74AsnPhe: 0.74 ± 0.259
2.056AsnGly: 2.056 ± 0.401
0.494AsnHis: 0.494 ± 0.23
0.823AsnIle: 0.823 ± 0.325
0.905AsnLys: 0.905 ± 0.277
1.069AsnLeu: 1.069 ± 0.363
0.411AsnMet: 0.411 ± 0.174
0.823AsnAsn: 0.823 ± 0.208
1.727AsnPro: 1.727 ± 0.45
1.398AsnGln: 1.398 ± 0.391
1.481AsnArg: 1.481 ± 0.405
1.974AsnSer: 1.974 ± 0.386
2.139AsnThr: 2.139 ± 0.354
2.632AsnVal: 2.632 ± 0.567
0.411AsnTrp: 0.411 ± 0.172
0.987AsnTyr: 0.987 ± 0.225
0.0AsnXaa: 0.0 ± 0.0
Pro
4.606ProAla: 4.606 ± 0.589
0.165ProCys: 0.165 ± 0.154
5.511ProAsp: 5.511 ± 0.64
4.113ProGlu: 4.113 ± 0.647
0.987ProPhe: 0.987 ± 0.318
2.55ProGly: 2.55 ± 0.413
0.905ProHis: 0.905 ± 0.228
2.139ProIle: 2.139 ± 0.515
1.481ProLys: 1.481 ± 0.421
3.126ProLeu: 3.126 ± 0.418
0.74ProMet: 0.74 ± 0.264
1.234ProAsn: 1.234 ± 0.342
2.139ProPro: 2.139 ± 0.533
1.645ProGln: 1.645 ± 0.426
2.303ProArg: 2.303 ± 0.493
4.606ProSer: 4.606 ± 0.571
3.948ProThr: 3.948 ± 0.592
3.29ProVal: 3.29 ± 0.546
0.74ProTrp: 0.74 ± 0.219
0.987ProTyr: 0.987 ± 0.264
0.0ProXaa: 0.0 ± 0.0
Gln
3.29GlnAla: 3.29 ± 0.409
0.082GlnCys: 0.082 ± 0.092
1.727GlnAsp: 1.727 ± 0.393
1.974GlnGlu: 1.974 ± 0.508
1.645GlnPhe: 1.645 ± 0.391
1.727GlnGly: 1.727 ± 0.283
1.069GlnHis: 1.069 ± 0.351
1.069GlnIle: 1.069 ± 0.344
0.658GlnLys: 0.658 ± 0.244
2.961GlnLeu: 2.961 ± 0.494
0.247GlnMet: 0.247 ± 0.151
1.481GlnAsn: 1.481 ± 0.343
2.056GlnPro: 2.056 ± 0.388
1.234GlnGln: 1.234 ± 0.382
2.468GlnArg: 2.468 ± 0.507
1.152GlnSer: 1.152 ± 0.258
3.208GlnThr: 3.208 ± 0.542
2.468GlnVal: 2.468 ± 0.459
1.069GlnTrp: 1.069 ± 0.411
1.481GlnTyr: 1.481 ± 0.348
0.0GlnXaa: 0.0 ± 0.0
Arg
5.511ArgAla: 5.511 ± 0.629
0.576ArgCys: 0.576 ± 0.254
6.087ArgAsp: 6.087 ± 0.815
6.745ArgGlu: 6.745 ± 0.929
1.974ArgPhe: 1.974 ± 0.482
4.524ArgGly: 4.524 ± 0.531
1.645ArgHis: 1.645 ± 0.374
2.961ArgIle: 2.961 ± 0.566
1.81ArgLys: 1.81 ± 0.374
5.922ArgLeu: 5.922 ± 0.798
1.069ArgMet: 1.069 ± 0.352
2.056ArgAsn: 2.056 ± 0.417
2.468ArgPro: 2.468 ± 0.408
2.632ArgGln: 2.632 ± 0.533
5.264ArgArg: 5.264 ± 0.749
4.606ArgSer: 4.606 ± 0.675
3.126ArgThr: 3.126 ± 0.499
4.935ArgVal: 4.935 ± 0.638
1.645ArgTrp: 1.645 ± 0.411
2.221ArgTyr: 2.221 ± 0.533
0.0ArgXaa: 0.0 ± 0.0
Ser
5.593SerAla: 5.593 ± 0.624
0.165SerCys: 0.165 ± 0.13
6.333SerAsp: 6.333 ± 0.739
5.758SerGlu: 5.758 ± 0.704
1.892SerPhe: 1.892 ± 0.415
6.498SerGly: 6.498 ± 1.149
1.152SerHis: 1.152 ± 0.251
3.372SerIle: 3.372 ± 0.621
1.645SerLys: 1.645 ± 0.417
4.03SerLeu: 4.03 ± 0.567
0.494SerMet: 0.494 ± 0.217
1.974SerAsn: 1.974 ± 0.48
2.55SerPro: 2.55 ± 0.468
1.481SerGln: 1.481 ± 0.405
2.961SerArg: 2.961 ± 0.443
3.208SerSer: 3.208 ± 0.539
4.771SerThr: 4.771 ± 0.576
4.442SerVal: 4.442 ± 0.575
1.152SerTrp: 1.152 ± 0.362
2.139SerTyr: 2.139 ± 0.466
0.0SerXaa: 0.0 ± 0.0
Thr
5.84ThrAla: 5.84 ± 0.619
0.494ThrCys: 0.494 ± 0.24
5.84ThrAsp: 5.84 ± 0.86
4.606ThrGlu: 4.606 ± 0.639
1.234ThrPhe: 1.234 ± 0.329
6.169ThrGly: 6.169 ± 0.971
1.398ThrHis: 1.398 ± 0.259
2.879ThrIle: 2.879 ± 0.431
1.069ThrLys: 1.069 ± 0.276
6.169ThrLeu: 6.169 ± 0.719
0.823ThrMet: 0.823 ± 0.267
2.714ThrAsn: 2.714 ± 0.425
4.853ThrPro: 4.853 ± 0.642
2.385ThrGln: 2.385 ± 0.573
4.688ThrArg: 4.688 ± 0.574
3.29ThrSer: 3.29 ± 0.453
5.511ThrThr: 5.511 ± 0.869
6.909ThrVal: 6.909 ± 0.956
0.987ThrTrp: 0.987 ± 0.293
2.056ThrTyr: 2.056 ± 0.437
0.0ThrXaa: 0.0 ± 0.0
Val
8.719ValAla: 8.719 ± 0.988
0.576ValCys: 0.576 ± 0.24
9.952ValAsp: 9.952 ± 0.913
7.567ValGlu: 7.567 ± 0.625
2.385ValPhe: 2.385 ± 0.433
6.498ValGly: 6.498 ± 0.801
1.152ValHis: 1.152 ± 0.353
2.385ValIle: 2.385 ± 0.368
1.316ValLys: 1.316 ± 0.233
4.442ValLeu: 4.442 ± 0.627
0.987ValMet: 0.987 ± 0.303
1.81ValAsn: 1.81 ± 0.339
2.961ValPro: 2.961 ± 0.463
2.056ValGln: 2.056 ± 0.365
5.346ValArg: 5.346 ± 0.606
5.429ValSer: 5.429 ± 0.816
5.182ValThr: 5.182 ± 0.59
6.087ValVal: 6.087 ± 0.855
0.494ValTrp: 0.494 ± 0.14
2.303ValTyr: 2.303 ± 0.468
0.0ValXaa: 0.0 ± 0.0
Trp
0.987TrpAla: 0.987 ± 0.336
0.0TrpCys: 0.0 ± 0.0
1.398TrpAsp: 1.398 ± 0.298
1.234TrpGlu: 1.234 ± 0.328
0.247TrpPhe: 0.247 ± 0.178
0.576TrpGly: 0.576 ± 0.211
0.247TrpHis: 0.247 ± 0.166
0.576TrpIle: 0.576 ± 0.252
0.0TrpLys: 0.0 ± 0.0
1.234TrpLeu: 1.234 ± 0.449
0.329TrpMet: 0.329 ± 0.178
0.411TrpAsn: 0.411 ± 0.128
0.658TrpPro: 0.658 ± 0.23
1.234TrpGln: 1.234 ± 0.298
1.892TrpArg: 1.892 ± 0.502
1.563TrpSer: 1.563 ± 0.369
1.316TrpThr: 1.316 ± 0.349
1.645TrpVal: 1.645 ± 0.301
0.576TrpTrp: 0.576 ± 0.203
0.905TrpTyr: 0.905 ± 0.328
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.55TyrAla: 2.55 ± 0.382
0.411TyrCys: 0.411 ± 0.221
3.619TyrAsp: 3.619 ± 0.605
2.879TyrGlu: 2.879 ± 0.637
1.152TyrPhe: 1.152 ± 0.366
3.372TyrGly: 3.372 ± 0.48
0.823TyrHis: 0.823 ± 0.245
0.823TyrIle: 0.823 ± 0.355
0.658TyrLys: 0.658 ± 0.23
1.563TyrLeu: 1.563 ± 0.336
0.329TyrMet: 0.329 ± 0.156
0.905TyrAsn: 0.905 ± 0.252
0.74TyrPro: 0.74 ± 0.276
1.152TyrGln: 1.152 ± 0.255
1.892TyrArg: 1.892 ± 0.398
1.152TyrSer: 1.152 ± 0.305
2.139TyrThr: 2.139 ± 0.6
2.139TyrVal: 2.139 ± 0.473
0.165TyrTrp: 0.165 ± 0.103
0.576TyrTyr: 0.576 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12159 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski